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| 1 | Preparation and Characteristic Identification of Monoclonal Antibody Against Sulfamethazine显示文摘Two artificial antigens were synthesized successfully by diazotizing method, sulfamethazine(SM2)-human serum albumin (HSA) was used for the immunogen, and SM2-ovalbumin(OVA) was used for the coating antigen. The coupled reaction was successful by confirmation of the ultraviolet scanning spectrometer, and the conjugation ratio of SM2 with HSA and OVA was 9:1 and 15:1, respectively. Using cell-fusion and limiting dilution method to reclone 5 times to get 3 hybridoma strains, which could stably secret monoclonal antibody (Mab), named CB7, BC4 and BB12. The subtype of BC4 Mab was IgG1 and chain, the molecular weight was 162 ku, the numbers of chromosomal were about 90, the affinity constant was 6.1×1012 M-1. No cross reactivity was seen between the Mab and the other 4 sulfonamides, as well as the 2 carries proteins. The Mab antibody had excellent stability. | DING Liangjun LI Jichang FU Rui ZHOU Yanjun HUO Guicheng | 2006 | Journal of Northeast Agricultural University(English Edition)2006，13，2： | 1 |
| 2 | TNF ‐α gene promoter polymorphisms contribute to periodontitis susceptibility: evidence from 46 studies显示文摘 | Cheng Ding Xiaowei Ji Xing Chen Yan Xu Liangjun Zhong | 2014 | J Clin Periodontol2014，，8： | 1 |
| 3 | Opportunistic Relaying Schemes for Amplify and Forward Bidirectional Relay Channels with Multi antenna Sources显示文摘We consider a two-way relay network where the Amplify-and-Forward (AF) protocol is adopted by all relays in this paper.The network consists of two multi-antenna source nodes and multiple distributed single-antenna relays.Two opportunistic relaying schemes are proposed to efficiently utilize the antennas of the source nodes and the relay nodes.In the first scheme,the best relay is selected out by a max-min-max criterion before transmitting.After that,at each source,only the antenna with the largest channel gain between itself and the best relay is activated to transmit and receive signals with full power.In the second scheme,assisted by the best relay which is selected by the typical max-min criterion,both source nodes use all their antennas to exchange data,and match filter beamforming techniques are employed at both source nodes.Further analyses show that all schemes can achieve the full diversity order,and the conclusions are not only mathematically demonstrated but numerically illustrated.System performance comparisons are carried out by numerical methods in terms of rate sum and outage probability,respectively.The beamforming assisted scheme can be found to be superior to the antenna selection scheme when accurate Channel State Information (CSI) is available at the transmitters.Otherwise,the latter is very suitable. | Wang Zibin Yuan Fu Ding Hong Xiang Liangjun Zheng Linhua | 2012 | China Communications2012，9，4： | 1 |
| 4 | Constructing ultrastable electrode/electrolyte interface for rapid potassium ion storage capability via salt chemistry and interfacial engineering显示文摘Conversion/alloying anode materials exhibiting high K storage capacities suffer from large volume variations and unstable electrode/electrolyte interfaces upon cycling.Herein,taking SnS/reduced graphene oxide(SnS/rGO)anodes as an example,the electrochemical performance of SnS/rGO could significantly be improved via employing potassium bis(fluorosulfonyl)imide(KFSI)salt in electrolytes and ultrathin TiO_(2) coating.KF-rich inorganic layer was demonstrated to help form robust SEI layer,which could suppress the side reactions to increase the Coulombic efficiency.The formed potassiated K_(x)TiO_(2) coating layer was constructed to boost charge transfer capability and K-ion diffusion kinetics.The as-prepared SnS/rGO@TiO_(2)-20 electrode in KFSI electrolyte delivers the high CE of 99.1%and 424 mAh·g^(−1) after 200 cycles with an ultrahigh capacity retention of 98.5%. | Sheng Wen Xin Gu Xiangwei Ding Li Zhang Pengcheng Dai Liangjun Li Dandan Liu Wenchao Zhang Xuebo Zhao Zaiping Guo | 2022 | Nano Research2022，15，3： | 0 |
| 5 | Study on ELISA for detection of sulfamethazine residues显示文摘In this study,various factors of ELISA for detection of sulfamethazine residues were explored,the coating antigen was diluted to 1:400,the best coating condition was at 4℃overnight,the working concentration of HRP-IgG enzyme conjugate was 1:7 000.The pre-incubation time and incubation time was 30 min and 120 min,respectively,the substrate solution working time was 20 min.Two moL·L^(-1) H_2SO_4 was used to stop the reaction and checked.A standard curve of direct competitive ELISA had been established to detect the sulfamethazine residues in milk.The detection limit of this method was 1.97 ng·mL^(-1).The mean concentration of sulfamethazine required to inhibit 30% antibody was 7.1 ng·mL^(-1).The linear range of the detection was 5-200 ng·mL^(-1). The recovery ratio was between 73.20% and 91.16%.The CV% of within array and between arrays was less than 10%. | LI Jichang HE Liang XU Shiwen HUO Guicheng DING Liangjun LIU Ning | 2007 | Journal of Northeast Agricultural University(English Edition)2007，14，3： | 0 |
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