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| 1 | Renin-angiotensin system in the pathogenesis of liver fibrosis显示文摘Hepatic fibrosis is considered a common response to many chronic hepatic injuries.It is a multifunctional process that involves several cell types,cytokines,chemokines and growth factors leading to a disruption of homeostatic mechanisms that maintain the liver ecosystem.In spite of many studies regarding the development of fibrosis,the understanding of the pathogenesis remains obscure.The hepatic tissue remodeling process is highly complex,resulting from the balance between collagen degradation and synthesis.Among the many mediators that take part in this process,the components of the Renin angiotensin system(RAS) have progressively assumed an important role.Angiotensin(Ang)□acts as a profibrotic mediator and Ang-(1-7),the newly recognized RAS component,appears to exert a counter-regulatory role in liver tissue.We briefly review the liver fibrosis process and current aspects of the RAS.This review also aims to discuss some experimental evidence regarding the participation of RAS mediators in the pathogenesis of liver fibrosis,focusing on the putative role of the ACE2-Ang-(1-7)-Mas receptor axis. | Regina Maria Pereira Robson Augusto Souza dos Santos Filipi Leles da Costa Dias Mauro Martins Teixeira Ana Cristina Simoes e Silva | 2009 | World Journal of Gastroenterology2009，15，21： | 37 |
| 2 | 从美国外科住院医师培训制度看如何改进我国临床研究生教育显示文摘临床研究生是我国继续医学教育的重要组成部分。借助美国住院医师培训制度的经验,对改进我国临床研究生教育,提高其临床工作能力提出一些建议。改变或摒弃目前临床硕士研究生的培养模式,适当降低临床博士研究生的科研要求,加强和完善博士生的临床考试和考核制度,对增加临床博士生毕业后的临床工作能力是有益的。将目前的临床研究生教育与专科医师培养制度衔接起来亦可以作为提高临床研究生工作能力的一种方法或作为全面推行专科医师培养制度的一个过渡阶段。 | 陈文生 张金洲 Dia Smiley | 2009 | 西北医学教育2009，17，2： | 34 |
| 3 | Long-term outcomes of hepatocellular carcinoma that underwent chemoembolization for bridging or downstaging显示文摘BACKGROUND Prospective study of 200 patients with hepatocellular carcinoma(HCC)that underwent liver transplant(LT)after drug-eluting beads transarterial chemoembolization(DEB-TACE)for downstaging versus bridging.Overall survival and tumor recurrence rates were calculated,eligibility for LT,time on the waiting list and radiological response were compared.After TACE,only patients within Milan Criteria(MC)were transplanted.More patients underwent LT in bridging group.Five-year post-transplant overall survival,recurrence-free survival has no difference between the groups.Complete response was observed more frequently in bridging group.Patients in DS group can achieve posttransplant survival and HCC recurrence-free probability,at five years,just like patients within MC in patients undergoing DEB-TACE.AIM To determine long-term outcomes of patients with HCC that underwent LT after DEB-TACE for downstaging vs bridging.METHODS Prospective cohort study of 200 patients included from April 2011 through June 2014.Bridging group included patients within MC.Downstaging group(out of MC)was divided in 5 subgroups(G1 to G5).Total tumor diameter was≤8 cm for G1,2,3,4(n=42)and was>8 cm for G5(n=22).Downstaging(n=64)and bridging(n=136)populations were not significantly different.Overall survival and tumor recurrence rates were calculated by the Kaplan-Meier method.Additionally,eligibility for LT,time on the waiting list until LT and radiological response were compared.RESULTS After TACE,only patients within MC were transplanted.More patients underwent LT in bridging group 65.9%(P=0.001).Downstaging population presented:higher number of nodules 2.81(P=0.001);larger total tumor diameter 8.09(P=0.001);multifocal HCC 78%(P=0.001);more post-transplantation recurrence 25%(P=0.02).Patients with maximal tumor diameter up to 7.05 cm were more likely to receive LT(P=0.005).Median time on the waiting list was significantly longer in downstaging group 10.6 mo(P=0.028).Five-year posttransplant overall survival was 73.5%in downstaging and 72.3%bridging groups(P=0.31),and recurrence-free survival was 62.1%in downstaging and 74.8%bridging groups(P=0.93).Radiological response:complete response was observed more frequently in bridging group(P=0.004).CONCLUSION Tumors initially exceeding the MC down-staged after DEB-TACE,can achieve post-transplant survival and HCC recurrence-free probability,at five years,just like patients within MC in patients undergoing DEB-TACE. | Breno Boueri Affonso Francisco Leonardo Galastri Joaquim Mauricio da Motta Leal Filho Felipe Nasser Priscila Mina Falsarella Rafael Noronha Cavalcante Marcio Dias de Almeida Guilherme Eduardo Goncalves Felga Leonardo Guedes Moreira Valle Nelson Wolosker | 2019 | World Journal of Gastroenterology2019，25，37： | 21 |
| 4 | Risk factors, surgical complications and graft survival in liver transplant recipients with early allograft dysfunction显示文摘Background: Early allograft dysfunction (EAD) is a severe complication after liver transplantation. The associated risk factors and complications have re-gained recent interest. This study investigated risk factors, survival and complications associated with EAD in a large liver transplant center in Latin America. Methods: Retrospective, unicenter, cohort, based on data from adult patients undergoing first deceaseddonor liver transplant from January 2009 to December 2013. EAD was defined by one or more of the following:(i) bilirubin ≥10 mg/dL on postoperative day 7;(ii) international normalized ratio ≥1.6 on postoperative day 7, and (iii) alanine aminotransferase or aspartate aminotransferase > 2000 IU/L within the first seven days after transplant. Results: A total of 602 patients were included;of these 34.2% developed EAD. Donor risk factors were male ( P = 0.007), age between 50 and 59 years ( P = 0.034), overweight ( P = 0.028) or grade I obesity ( P = 0.012), sodium > 157 mmol/L ( P = 0.002) and grade IV ischemia/reperfusion injury ( P = 0.002). Cold ischemia time ≥10 h ( P = 0.008) and warm ischemia time ≥40 min ( P = 0.013) were the surgical factors. Male ( P < 0.001) was the only recipient protective factor. Compared with the non-EAD group, patients with EAD were submitted to more reoperations (24.3% vs. 13.4%, P = 0.001) and had higher graft loss rates (37.9% vs. 21.2%, P < 0.001), with similar patient survival rates ( P = 0.238). Conclusions: EAD risk factors are related to donor, surgical procedure and recipient. Donor risk factors for EAD were male, age between 50 and 59 years, donor overweight or grade Ⅰ obesity, sodium > 157 mmol/L and grade Ⅳ ischemia/reperfusion injury. Cold ischemia time ≥10 h and warm ischemia time ≥40 min were the surgical risk factors. Male was the only recipient protective factor. Patients with EAD had higher reoperations and graft loss rates. | Douglas Bastos-Neves Paolo Rogerio de Oliveira Salvalaggio Marcio Dias de Almeida | 2019 | Hepatobiliary & Pancreatic Diseases International2019，18，5： | 15 |
| 5 | 非线性准则下三维加筋边坡稳定性的上限分析显示文摘为了研究非线性摩尔-库仑破坏准则下的三维加筋边坡在均匀加筋和三角形加筋2种加筋模式下的稳定性,采用极限分析上限理论,构建加筋边坡破坏的三维破坏机构,推导不同加筋模式下的筋材内能耗散方程,并根据上限定理将三维加筋边坡的稳定性问题转化为显式优化问题,得到三维边坡稳定性指标的计算公式。采用MATLAB软件计算,将结果与已有研究成果进行比较,验证了所提出计算方法的正确性,并进一步讨论了非线性参数m、宽高比B/H、不同加筋模式以及不同加筋强度k0对三维边坡稳定性的影响。结果表明:稳定性系数Ns随着m的增加而非线性减小,在坡角较小(不大于60°)的情况下,m对边坡稳定性系数Ns的影响较大,边坡的非线性特性比较明显;稳定性系数Ns随着B/H的增加而减小,在B/H<5尤其B/H<2时,稳定性系数下降速率较快,B/H越接近10时稳定性系数逐渐趋于稳定;三角形加筋模式的加固效果优于均匀加筋模式,稳定系数性Ns随着加筋强度k0的增加而线性增大,但变化规律并不显著。在实际工程中,建议B/H较小时,要对加筋边坡进行三维分析以符合工程实际;坡角较小时需要考虑土体的非线性破坏特征,选取合适的非线性参数可避免不安全的设计;在工程条件允许的情况下,优先选择三角形加筋模式进行边坡加固。 | 侯超群 邓欣 孙志彬 DIAS D 许敬叔 | 2018 | 中国公路学报2018，31，2： | 13 |
| 6 | Keratin-Based materials for biomedical applications显示文摘Keratin constitutes the major component of the feather,hair,hooves,horns,and wool represents a group of biological material having high cysteine content(7–13%)as compared to other structural proteins.Keratin-based biomaterials have been investigated extensively over the past few decades due to their intrinsic biological properties and excellent biocompatibility.Unlike other natural polymers such as starch,collagen,chitosan,the complex three-dimensional structure of keratin requires the use of harsh chemical conditions for their dissolution and extraction.The most commonly used methods for keratin extraction are oxidation,reduction,steam explosion,microbial method,microwave irradiation and use of ionic liquids.Keratin-based materials have been used extensively for various biomedical applications such as drug delivery,wound healing,tissue engineering.This review covers the structure,properties,history of keratin research,methods of extraction and some recent advancements related to the use of keratin derived biomaterials in the form of a 3-D scaffold,films,fibers,and hydrogels. | Sandleen Feroz Nawshad Muhammad Jithendra Ratnayake George Dias | 2020 | Bioactive Materials2020，5，3： | 13 |
| 7 | 软土地面中机械化双叠隧道的三维数值模拟(英文)显示文摘研究目的:通过三维数值模拟,研究隧道施工过程对双叠隧道的影响。创新要点:使用全三维数值模拟方法研究软土中双叠隧道施工对先挖隧道或地面的影响。研究方法:1.运用FLAC软件创建双叠隧道的三维数值模型(图1);2.分情况模拟机械化双叠隧道的挖掘过程;3.研究不同情况下的地面沉降,水平地面位移,以及隧道衬砌的法向位移、法向力、纵向力和弯曲力矩等。重要结论:1.新隧道施工对现有的隧道有很大的影响,最大影响出现在先挖上层隧道的情况下;2.一般来说,上层隧道的挖掘会比下层隧道产生更大的地面沉降;3.下层隧道产生的法向力总是比上层隧道大;4.在多数情况下,下层隧道产生的法向位移和弯曲力矩要比上层隧道小。 | Ngoc-Anh DO Daniel DIAS Pierpaolo ORESTE | 2014 | Journal of Zhejiang University-Science A(Applied Physics & Engineering)2014，15，11： | 12 |
| 8 | Arabidopsis AT-hook Protein TEK Positively Regulates the Expression of Arabinogalactan Proteins for Nexine Formation显示文摘Nexine 是花粉墙的保存的层。我们以前报导 nexine 层在经由在钩(TEK ) 基因的 Arabidopsis TRANSPOSABLE 元素 SILENCING 的猛烈异种是不在的。在这研究,我们在花粉开发调查了 TEK 的分子的规章的功能并且识别了作为 TEK 的直接目标编码 Arabinogalactan 蛋白质(AGP ) 的基因,它为 nexine 形成是必要的。tek 和 TEK-SRDX 的 Phenotypic 类似转基因的线建议 TEK 在花药开发在 transcriptional 激活起一个作用。Microarray 分析在 tek 识别了 661 基因 downregulated 的一个总数,包括编码 AGP, AGP6, AGP11, AGP23,和 AGP40 的四基因。Electrophoretic 活动性移动试金证明 TEK 能直接绑原子矩阵附件区域(3 月) 和 AGP6 的倡导者。PCR 分析跟随的染色质 immunoprecipitation 证明 TEK 在四 AGP 基因的倡导者被充实。TEK 倡导者在 tek 驾驶的 AGP6 的表示部分救了 nexine 形成和植物富饶。这些结果显示 TEK 直接在花药调整 AGP 表示控制 nexine 层形成。我们也建议 glycoproteins 可能是在花粉墙中的 nexine 层的必要部件。 | Qi-Shi dia Jun Zhu Xiao-Feng Xu Yue Lou Zhan-Lin Zhang Zhi-Ping Zhang Zhong-Nan Yang | 2015 | Molecular Plant2015，8，2： | 10 |
| 9 | Behavior of noncircular tunnels excavated in stratified rock masses-Case of underground coal mines显示文摘The amount of tunnels excavated along stratified/sedimentary rock masses in Quangninh coal mine area,Vietnam, is gradually increasing. Rock mass in Quangninh is characterized by beddings between rock layers. The behavior of stratified rock masses surrounding the tunnels depends on both the intact rock and the beddings between rock layers. The main characteristics of stratified rock masses that need to be considered are their heterogeneity and anisotropy. Depending on the dip angle of rock layers, movements and failure zones developed surrounding the tunnels can be asymmetrical over the vertical axis of tunnel. This asymmetry causes adverse behaviors of the tunnel structures. The objective of this study is to highlight convergences and yielded zones developed in rock masses surrounding noncircular tunnels in Quangninh coal mine area using a finite element method. The presence of bedding joints is explicitly simulated. The numerical results indicated that with the increase in dip angle of bedding joints, the stress asymmetry over the tunnel vertical axis increases. It gradually leads to an asymmetry of the failure zone surrounding the tunnel. An increase of rock mass quality means a decrease of rock mass sensitivity to the discontinuities. In addition,a dip angle of the bedding joints of approximately 45° could be considered as the critical angle at which the rock mass mechanism changes between sliding and bending. | Ngoc Anh Do Daniel Dias Van Diep Dinh Tien Tung Tran Van Canh Dao Viet Doan Dao Phuc Nhan Nguyen | 2019 | Journal of Rock Mechanics and Geotechnical Engineering2019，11，1： | 9 |
| 10 | Toll-like receptor 9 polymorphisms and Helicobacter pylori influence gene expression and risk of gastric carcinogenesis in the Brazilian population显示文摘BACKGROUND Toll-like receptors(TLRs)are the first line of host defense,and are involved in Helicobacter pylori(H.pylori)recognition and activation of both inflammatory and carcinogenic processes.The presence of single nucleotide polymorphisms(SNPs)in genes that activate the immune response may modulate the risk of precancerous lesions and gastric cancer(GC).Among them,Toll-like receptor 9(TLR9)polymorphisms have emerged with a risk factor of infectious diseases and cancer,however the studies are still inconclusive.AIM To evaluate whether TLR9 rs5743836 and rs187084 SNPs contribute to the risk of gastric carcinogenesis,and its influence on mRNA expression.METHODS A case-control study was conducted to evaluate two TLR9 SNPs(TLR9-1237 TCrs5743836 and TLR9-1486 CT-rs187084)in chronic gastritis(CG)and GC patients.A total of 609 DNA samples of peripheral blood[248 CG,161 GC,and 200 samples from healthy individuals(C)]were genotyped by polymerase chain reaction-restriction fragment length polymorphism.All samples were tested for the H.pylori infection using Hpx1 and Hpx2 primers.Quantitative polymerase chain reaction by TaqMan?assay was used to quantify TLR9 mRNA from fresh gastric tissues(48 GC,26 CG,and 14 C).RESULTS For TLR9-1237,the TC+CC or CC genotypes were associated with a higher risk of GC than C[recessive model odds ratio(OR)=5.01,95%confidence interval(CI):2.52-9.94,P<0.0001],and the CG(recessive model OR=4.63;95%CI:2.44-8.79,P<0.0001)groups.For TLR9-1486,an association between the CT+TT genotypes and increased risk of both GC(dominant model OR=2.72,95%CI:1.57-4.72,P<0.0001)and CG(dominant model OR=1.79,95%CI:1.15-2.79,P=0.0094)was observed when compared to the C group.Moreover,the presence of TLR9-1237 TC/CC+TLR9-1486 CC genotypes potentiate the risk for this neoplasm(OR=18.57;95%CI:5.06-68.15,P<0.0001).The TLR9 mRNA level was significantly higher in the GC group(RQ=9.24,P<0.0001)in relation to the CG group(RQ=1.55,P=0.0010)and normal mucosa(RQ=1.0).When the samples were grouped according to the polymorphic genotypes and the presence of H.pylori infection,an influence of TLR9-1237 TC+CC polymorphic genotypes(P=0.0083)and H.pylori infection(P<0.0001)was observed on the upregulation of mRNA expression.CONCLUSION Our findings show that TLR9 rs5743836 and rs187084 polymorphisms are associated with a higher risk of carcinogenesis gastric,and that TLR9 mRNA levels can be modulated by TLR9-1237 TC+CC variant genotypes and H.pylori infection. | Manoela Dias Susi de Matos Lourenco Caroline Lucas Trevizani Rasmussen Spencer Luis Marques Payao Ana Flavia Teixeira Rossi Ana Elizabete Silva Juliana Garcia de Oliveira-Cucolo | 2019 | World Journal of Gastrointestinal Oncology2019，11，11： | 9 |
| 11 | 人参皂苷Rg1对慢性束缚应激大鼠模型的抗抑郁作用(英文)显示文摘目的探讨人参皂苷Rg1对大鼠慢性束缚应激(CRS)所致抑郁样行为的改善作用及其潜在机制。方法将40只雄性Wistar大鼠根据糖水偏爱指数分为4组:对照组,模型组,Rg1低、高剂量组(5和10 mg/kg)。除对照组外,其他组所有实验大鼠均接受慢性束缚应激(CRS,6 h/d),持续28 d。束缚应激造模14 d后,开始腹腔注射给药。造模结束后,通过旷场实验、糖水偏爱实验、强迫游泳实验和Morris水迷宫实验检测其行为学改变。此外,使用ELISA试剂盒分析了血清皮质激素(CORT)的水平和抗氧化防御生物标记物(SOD,MDA和GSH-x)的活性。通过超高效液相色谱串联质谱法(UPLC-MS/MS)测量前额叶皮层(PFC)中神经递质(5-HT,5-HIAA,NE,Ach,GABA和Glu)的水平。用蛋白质印迹法检测前额皮层中BDNF,Trkb,Bax和Bcl-2的蛋白表达。结果人参皂苷Rg1的慢性治疗可显著减轻抑郁症样的行为(快感,行为绝望和学习记忆减退),表现为Rg1显著增加糖水偏爱实验中的蔗糖消耗,减少强迫游泳实验的不动时间和提高动物在Morris水迷宫中的表现。此外,慢性束缚应激显著改变了动物前额皮层中的神经递质(5-HT,5-HIAA,Ach,GABA和Glu)水平和抗氧化剂防御生物标志物(SOD,MDA和GSH-x)的活性以及血清中的皮质酮水平,而人参皂苷Rg1可以使这些改变恢复正常水平。同时,人参皂苷Rg1处理可显著逆转CRS大鼠PFC中BDNF,Trkb和Bcl-2的减少以及Bax表达的增加。结论人参皂苷Rg1可以减轻CRS诱导的抑郁样行为,部分通过调节神经递质水平和HPA功能,拮抗氧化应激和凋亡,恢复PFC中的BDNF-TrkB信号传导来介导。本研究为Rg1抗抑郁研究提供了更多的科学依据。 | 姜宁 吕静薇 王海霞 黄红 王琼 陈善广 曲丽娜 Alberto Carlos Pires Dias 刘新民 | 2019 | Digital Chinese Medicine2019，2，4： | 8 |
| 12 | Short-term complementary and alternative medicine on quality of life in women with fibromyalgia显示文摘BACKGROUND:Fibromyalgia(FMS)is a syndrome characterized by chronic widespread musculoskeletal pain,whose etiology is not completely understood.Different therapeutic approaches have been used with inconsistent results.This observation does not invalidate the continued search for alternative treatments aimed at improving quality of life(Qo L)in FMS.OBJECTIVE:This study compared three classical traditional Chinese medicine(TCM)therapies:acupuncture(AC),electroacupuncture(EAC)and moxibustion(MX)in the management of pain and promotion of Qo L in FMS patients.DESIGN,SETTING,PARTICIPANTS AND INTERVENTIONS:A preliminary,group-assigned,comparative study enrolled 30 women,mean age(46.90±9.24)years(range 20–60 years),who met the 1990 American College of Rheumatology criteria for FMS diagnosis and a pain-pressure threshold(PPT)< 4 kg/cm^2.The study was conducted in a teaching tertiary-care medical institution from May 2010 through April 2012.AC,EAC and MX were delivered for 30 min,once a week,for 8 weeks,bilaterally at Neiguan(PC6),Hegu(LI4),Yanglingquan(GB34),Sanyinjiao(SP6)and Taichong(LR3)acupoints.MAIN OUTCOME MEASURES:Each week,immediately before treatment and after treatment,subjects were tested for PPTs,Wong-Baker Faces Pain Scale(WBFPS;for pain intensity)and Medical Outcomes Study 36-item Short Form Health Survey(SF-36:for Qo L).RESULTS:There was no signifi cant improvement in pain or reduction of tender points in any of the groups studied,at the end of the 8th session.Signifi cant improvement of Qo L was perceived in vitality(after AC treatment)and in mental health(after EAC and MX treatments).CONCLUSION:TCM therapies(AC,EAC and MX)promoted an improvement in the Qo L in two areas(vitality and mental health)in FMS women.Further large-scale clinical trials are required to confi rm this effect. | Paulo Araujo Dias Andre Brito Bastos Guimaraes Andrea de Oliveira Albuquerque Karoline Lucas de Oliveira Maria Luzete Costa Cavalcante Sergio Botelho Guimaraes | 2016 | Journal of Integrative Medicine2016，14，1： | 7 |
| 13 | 河南省和山东省抗结核药品管理评价显示文摘目的:评价我国抗结核药品供应系统的绩效。方法:河南省和山东省各随机抽取4个地市和20个县,用13个量化指标通过现场问卷调查获得数据。结果:目前使用的6种抗结核药品均属于国家基本药物,有3种属于世界卫生组织基本药物;各个项目最近3次供货每个批号的药品都有质量检验报告,过去1年药品质量检验不合格率为0%,各项目药品招标采购的价格均低于国际价格;一些机构存在药品缺货、过期和账物相符率低的情况,药品库存控制不合理;一些机构未严格执行标准短程化疗方案;县级药品的信息报告准确率比较低。结论:我国建立了严格的药品质量控制体系,但复治涂阳患者药品的库存控制方法需要进一步研究,且应强化药品管理人员的能力,提高药品信息报告的准确性。 | 赖钰基 王林 王倪 徐敏 刘剑君 成诗明 马士文 李仁忠 Vim Dias Antonio Campopiano | 2007 | 中国药房2007，18，25： | 7 |
| 14 | Animal models of acute gastric mucosal injury: Macroscopic and microscopic evaluation显示文摘Acute gastric mucosal injury is a common disorder of the gastrointestinal tract and the search for new therapeutics is ongoing. The aim of this study is to update and expand the information related to the most widely used rat models of acute gastric ulcer, the ethanol‐induced ulcer and the indomethacin‐induced ulcer. These two models are compared in terms of macroscopic and microscopic features. Experimentally, ethanol was given orally in a single dose and indomethacin was subcutaneously injected into male Wistar rats. After ulcerative challenges, the stomachs were removed and visually inspected. Anti‐ulcerative drugs were used to validate the models. Histological analysis of the stomachs determined the microscopic score. The methodology used for model evaluation applied to macroscopic and microscopic gastric lesions. With these methods it was possible to induce lesions in the gastric mucosa. Microscopic evaluation permitted assessment of the inflammatory and apoptotic impact in the mucosa not observable by macroscopic evaluation. Groups of animals were treated with two standard drugs: sulcralfate suspension or lansoprazole solution. Both drugs reduced macroscopic and microscopic lesions, particularly the hemorrhagic ones. Both models induced acute gastric mucosal injury and no single evaluation method can address all the aspects of the pathology of gastric lesions. As a complement to macroscopic evaluation, microscopy appears to be a relevant tool to selectively identify specific aspects of the development of mucosal injury, quantify the extent of lesions, and contribute to an appropriate interpretation of results. The score systems established here offer a reliable method for testing antiulcer drugs. | Sandra Simoes Rui Lopes Maria Celeste Dias Campos Maria Joao Marruz Maria Eugenia Meirinhos da Cruz Luisa Corvo | 2019 | Animal Models and Experimental Medicine2019，2，2： | 7 |
| 15 | Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability显示文摘AIM:To verify the methylation status of CDH1, DAPK, COX2, hMLH1 and CDKN2A genes and to evaluate their association with Helicobacter pylori (H. pylori)-cagA+ and Epstein Barr virus (EBV) infections in gastric adenocarcinomas.METHODS: Methylation-specific PCR (MSP) assay was performed in 89 primary gastric carcinomas (intestinal and diffuse types). Microsatellite instability (MSI) analysis was performed using the BAT26 primer set and PCR products were analyzed with the ABI PRISM 3100 Genetic Analyzer using Genescan 3.7 software (Applied Biosystems). Detection of H. pylori and genotyping were performed by PCR, using specifi c primers for ureaseC and cagA genes. The presence of EBV was assessed by in situ hybridization. Statistical analyses were performed using the χ2 or Fisher's exact test.RESULTS: The most frequent hypermethylated gene was COX-2 (63.5%) followed by DAPK (55.7%), CDH1 (51%), CDKN2A (36%) and hMLH1 (30.3%). Intestinal and diffuse adenocarcinomas showed different methylation profiles and there was an association between methylation of E-CDH1 and H. pylori-cagA+ in the intestinal adenocarcinoma type. MSI was correlated with hMLH1 methylation. There was an inverse correlation between DAPK hypermethylation and MSI.CONCLUSION: We found a strong association between CDH1 methylation and H. pylori-cagA+ in intestinal-type gastric cancer, association of MSI and better prognosis and an heterogeneous COX-2 overexpression. | Adriana Camargo Ferrasi Nídia Alice Pinheiro Silvia Helena Barem Rabenhorst Otávia Luisa Caballero Maria Aparecida Marchesan Rodrigues Fabrício de Carvalho Celso Vieira de Souza Leite Marcia Valéria Pitombeira Ferreira Marcos Aurélio Pessoa Barros Maria Inês de Moura Campos Pardini | 2010 | World Journal of Gastroenterology2010，16，3： | 6 |
| 16 | Infliximab to treat severe ulcerative colitis显示文摘A 48-year-old female with severe ulcerative colitis refractory to conventional therapy was referred to our facility for management. The patient showed extensive ulcerative colitis since the age of 20 years and had failed therapy with 5-aminosalicylic acid agents and azathioprine. The disease remained active despite treatment with steroids and cyclosporine. The clinical and endoscopic parameters were consistent with severe disease. Infectious precipitants were ruled out. Given the severity of the disease and in order to avoid a colectomy, we started the patient on infliximab therapy. A dramatic clinical and endoscopic response was observed and she remained in remission at the end of a 1-year follow-up period. We discuss findings in the literature regarding the use ofinfliximab therapy in patients with ulcerative colitis who have failed steroids and cyclosporine. | Dídia Bisamra Cury Marcelo de Souza Cury Geraldo Vinicius Hemerly Elias Sender Jankiel Mizsputen | 2009 | World Journal of Gastroenterology2009，15，14： | 6 |
| 17 | Relationship between Th17 immune response and cancer显示文摘Cancer is the second leading cause of death worldwide and epidemiological projections predict growing cancer mortality rates in the next decades.Cancer has a close relationship with the immune system and,although Th17 cells are known to play roles in the immune response against microorganisms and in autoimmunity,studies have emphasized their roles in cancer pathogenesis.The Th17 immune response profile is involved in several types of cancer including urogenital,respiratory,gastrointestinal,and skin cancers.This type of immune response exerts pro and antitumor functions through several mechanisms,depending on the context of each tumor,including the protumor angiogenesis and exhaustion of T cells and the antitumor recruitment of T cells and neutrophils to the tumor microenvironment.Among other factors,the paradoxical behavior of Th17 cells in this setting has been attributed to its plasticity potential,which makes possible their conversion into other types of T cells such as Th17/Treg and Th17/Th1 cells.Interleukin(IL)-17 stands out among Th17-related cytokines since it modulates pathways and interacts with other cell profiles in the tumor microenvironment,which allow Th17 cells to prevail in tumors.Moreover,the IL-17 is able to mediate pro and antitumor processes that influence the development and progression of various cancers,being associated with variable clinical outcomes.The understanding of the relationship between the Th17 immune response and cancer as well as the singularities of carcinogenic processes in each type of tumor is crucial for the identification of new therapeutic targets. | Hanna Santos Marques Breno Bittencourt de Brito Filipe Antônio França da Silva Maria Luísa Cordeiro Santos Júlio César Braga de Souza Thiago Macêdo Lopes Correia Luana Weber Lopes Nayara Silva de Macêdo Neres Rafael Santos Dantas Miranda Dórea Anna Carolina Saúde Dantas Lorena Lôbo Brito Morbeck Iasmin Souza Lima Amanda Alves de Almeida Maiara Raulina de Jesus Dias Fabrício Freire de Melo | 2021 | World Journal of Clinical Oncology2021，12，10： | 5 |
| 18 | Effect of Using Magnetic Brackish Water on Irrigated Bell Pepper Crop (Capsicum annuum L.) Characteristics in Lower Jordan Valley/West Bank显示文摘 | Amer Marei Dia Rdaydeh Dia Karajeh Nawaf Abu-Khalaf | 2014 | Journal of Agricultural Science and Technology(A)2014，4，10： | 5 |
| 19 | Characteristics of wave amplitude and currents in South China Sea induced by a virtual extreme tsunami显示文摘This paper investigates the potential extreme tsunami hazards of the worst case scenario of the magnitude M_w=9.30 in South China Sea(SCS)as the Manila Trench is becoming one of the most hazardous tsunami source regions.Using nonlinear shallow water equations model,the time series of surface elevation,arrival time,spatial distributions of maximum wave amplitude and velocity distribution are presented.The characteristics of wave and currents are analyzed.The numerical results indicate that most of the energy of tsunami wave distributes in central and north part of SCS.The offshore regions around SCS will be influenced significantly by the tsunami currents generated by an earthquake in the Manila subduction zone.The maximum wave amplitude near Guangdong Province,Hainan Island,and Taiwan Island exceeds 4 m and velocities at the majority of measured locations near coast exceeds 2 m/s.Nested grid with high resolution is used to study the impacts of the tsunami on Hainan Island,Taiwan Island,and Lingding Bay.The regions with high hazard risk due to strong currents are identified.Finally,a fast tsunami warning method in SCS is developed and discussed,which can provide tsunami warning information in 5 min. | 任智源 赵曦 王本龙 Frédéric Dias 刘桦 | 2017 | Journal of Hydrodynamics2017，29，3： | 5 |
| 20 | Three-dimensional finite difference analysis of shallow sprayed concrete tunnels crossing a reverse fault or a normal fault: A parametric study显示文摘Urban tunnels crossing faults are always at the risk of severe damages.In this paper,the efects of a reverse and a normal fault movement on a transversely crossing shallow shotcreted tunnel are investigated by 3D finite difference analysis.After verifying the accuracy of the numerical simulation predictions with the centrifuge physical model results,a parametric study is then conducted.That is,theleffects of various parameters such as the sprayed concrete thickness,the geo-mechanical properties of soil,the tunnel depth,and the fault plane dip angle are studied on the displacements of the ground surface and the tunnel structure,and on the plastic strains of the soil mass around tunnel.The results of each case of reverse and normal faulting are independently discussed and then compared with each other.It is obtained that deeper tunnels show greater displacements for both types of faulting. | Masoud RANJBARNIA Milad ZAHERI Daniel DIAS | 2020 | Frontiers of Structural and Civil Engineering2020，14，4： | 4 |
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