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您的检索式：作者名="DENG SuHui"
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| 1 | Nanoprobes for super-resolution fluorescence imaging at the nanoscale显示文摘Compared with other imaging techniques,fluorescence microscopy has become an essential tool to study cell biology due to its high compatibility with living cells.Owing to the resolution limit set by the diffraction of light,fluorescence microscopy could not resolve the nanostructures in the range of<200 nm.Recently,many techniques have been emerged to overcome the diffraction barrier,providing nanometer spatial resolution.In the course of development,the progress in fluorescent probes has helped to promote the development of the high-resolution fluorescence nanoscopy.Here,we describe the contributions of the fluorescent probes to far-field super resolution imaging,focusing on concepts of the existing super-resolution nanoscopy based on the photophysics of fluorescent nanoprobes,like photoswitching,bleaching and blinking.Fluorescent probe technology is crucial in the design and implementation of super-resolution imaging methods. | HOU ShangGuo LIANG Le DENG SuHui CHEN JianFang HUANG Qing CHENG Ya FAN ChunHai | 2014 | Science China Chemistry2014，57，1： | 4 |
| 2 | Superresolution imaging of telomeres with continuous wave stimulated emission depletion (STED) microscope显示文摘The significant role of telomeres in cells has attracted much attention since they were discovered.Fluorescence imaging is an effective method to study subcellular structures like telomeres.However,the diffraction limit of traditional optical microscope hampers further investigation on them.Recent progress on superresolution fluorescence microscopy has broken this limit.In this work,we used stimulated emission depletion(STED) microscope to observe fluorescence-labeled telomeres in interphase cell nuclei.The results showed that the size of fluorescent puncta representing telomeres under the STED microscope was much smaller than that under the confocal microscope.Two adjacent telomeres were clearly separated via STED imaging,which could hardly be discriminated by confocal microscopy due to the diffraction limit.We conclude that STED microscope is a more powerful tool that enable us to obtain detailed information about telomeres. | Shaopeng Wang Suhui Deng Xiaoqing Cai Shangguo Hou Jiajun Li Zhaoshuai Gao Jiang Li Lihua Wang Chunhai Fan | 2016 | Science China Chemistry2016，59，11： | 3 |
| 3 | Sub-diffraction-limit cell imaging using a super-resolution microscope with simplified pulse synchronization显示文摘Stimulated emission depletion(STED) microscope is one of the most prominent super-resolution bio-imaging instruments, which holds great promise for ultrahigh-resolution imaging of cells. To construct a STED microscope, it is challenging to realize temporal synchronization between the excitation pulses and the depletion pulses. In this study, we present a simple and low-cost method to achieve pulse synchronization by using a condensed fluorescent dye as a depletion indicator. By using this method, almost all the confocal microscopes can be upgraded to a STED system without losing its original functions. After the pulse synchronization,our STED system achieved sub-100-nm resolution for fluorescent nanospheres and single-cell imaging. | Zhaoshuai Gao Suhui Deng Jiang Li Kun Wang Jiajun Li Lihua Wang Chunhai Fan | 2017 | Science China Chemistry2017，60，10： | 2 |
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