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| 1 | The deubiquitinase OTUB1 augments NF-κB-dependent immune responses in dendritic cells in infection and inflammation by stabilizing UBC13显示文摘Dendritic cells(DCs)are indispensable for defense against pathogens but may also contribute to immunopathology.Activation of DCs upon the sensing of pathogens by Toll-like receptors(TLRs)is largely mediated by pattern recognition receptor/nuclear factor-κB(NF-κB)signaling and depends on the appropriate ubiquitination of the respective signaling molecules.However,the ubiquitinating and deubiquitinating enzymes involved and their interactions are only incompletely understood.Here,we reveal that the deubiquitinase OTU domain,ubiquitin aldehyde binding 1(OTUB1)is upregulated in DCs upon murine Toxoplasma gondii infection and lipopolysaccharide challenge.Stimulation of DCs with the TLR11/12 ligand T.gondii profilin and the TLR4 ligand lipopolysaccharide induced an increase in NF-κB activation in OTUB1-competent cells,resulting in elevated interleukin-6(IL-6),IL-12,and tumor necrosis factor(TNF)production,which was also observed upon the specific stimulation of TLR2,TLR3,TLR7,and TLR9.Mechanistically,OTUB1 promoted NF-κB activity in DCs by K48-linked deubiquitination and stabilization of the E2-conjugating enzyme UBC13,resulting in increased K63-linked ubiquitination of IRAK1(IL-1 receptor-associated kinase 1)and TRAF6(TNF receptor-associated factor 6).Consequently,DC-specific deletion of OTUB1 impaired the production of cytokines,in particular IL-12,by DCs over the first 2 days of T.gondii infection,resulting in the diminished production of protective interferon-γ(IFN-γ)by natural killer cells,impaired control of parasite replication,and,finally,death from chronic T.encephalitis,all of which could be prevented by low-dose IL-12 treatment in the first 3 days of infection.In contrast,impaired OTUB1-deficient DC activation and cytokine production by OTUB1-deficient DCs protected mice from lipopolysaccharide-induced immunopathology.Collectively,these findings identify OTUB1 as a potent novel regulator of DCs during infectious and inflammatory diseases. | Floriana Mulas Xu Wang Shanshan Song Gopala Nishanth Wenjing Yi Anna Brunn Pia-Katharina Larsen Berend Isermann Ulrich Kalinke Antonio Barragan Michael Naumann Martina Deckert Dirk Schlüter | 2021 | Cellular & Molecular Immunology2021，18，6： | 4 |
| 2 | Plasma phospholipid transfer protein (PLTP) modulates adaptive immune functions through alternation of T helper cell polarization显示文摘客观:血浆 phospholipid 转移蛋白质(PLTP ) 是脂蛋白新陈代谢的一个关键决定因素,并且动物和人的研究收敛显示 PLTP 支持 atherogenesis 和它的 thromboembolic 复杂并发症。而且, PLTP 调制发炎和有免疫力的回答,这最近被报导了。尽管从我们的组的更早的研究证明 PLTP 能修改巨噬细胞激活,在 T-cell-mediated 有免疫力的回答的调整的 PLTP 的含意从来没被调查过并且因此在现在的学习被探讨。途径和结果:在现在的学习,我们证明在老鼠的那 PLTP 缺乏在 CD4 + Th0 房间极化上有深刻效果,与向在正常、病理学的条件下面的反煽动性的 Th2 显型的移动。在接触超敏性的一个模型,对有 hapten-2,4-dinitrofluorobenzene (DNFB ) 的皮肤促进感受性的显著地损害的回答在 PLTP 缺乏的老鼠被观察与相比野类型(WT ) 老鼠。有趣地,在老鼠的 PLTP 缺乏没在外部血在全部的白血房间,淋巴细胞, granulocytes,或单核白血球的计数上施加效果。而且, PLTP 缺乏没修改 CD4 + 和 CD8 + T 淋巴细胞子集的数量。然而, PLTP 缺乏,与 Th2 显型的 upregulation 联系了,被重要减少在 pro-Th1 cytokine interleukin 的生产伴随 18 由附件房间。结论:第一次,这个工作向支持 inflammatory Th1 显型在 CD4 + T 房间的极化为 PLTP 报导一个生理的角色。 | Catherine Desrumau Stephanie Lemaire-Ewing Nicolas Ogier Akadiri Yessoufou Arlette Hammann Anabelle Sequeira-Le Grand Valerie Deckert Jean-Paul Pais de Barros Naig Le Guern Julien Guy Naim A Khan Laurent Lagrost | 2016 | Cellular & Molecular Immunology2016，13，6： | 3 |
| 3 | The glycosylphos-phatidylinositol-anchored CD59 protein stimmulates both T cell receptor ZAP-70 dependent and indepentent signaling pathways in T cells显示文摘 | Deckert M Ticchioni M Mari B | 1995 | Immunol1995，5，： | 1 |
| 4 | Primary CNS lymphoma : clinical presentation, pathological classification, molecular pathogenesis and treatment 显示文摘 | Schlegel U Schmidt-Wolf IG Deckert M | 2000 | J Neurol Sci2000，181，12： | 1 |
| 5 | CD59 molecule:a second ligand for CD2 in T cell adhesion显示文摘 | Deckert M Kubar J Zoccola D | 1992 | Eur J Immunol1992，22，11： | 1 |
| 6 | Regional ontogenetic profile of central and peripheral benzodiazepine receptors in the guinea pig brain显示文摘 | Daval JL Deckert J Nakajima T | 1988 | Neurosci Lett1988，92，1： | 1 |
| 7 | Current strategies in the diagnosis of diffuse large B-cell lymphoma of the central nervous system 显示文摘 | Baraniskin A Deckert M Schulte-Altedomeburg G | 2012 | Br J Haematol2012，156，： | 1 |
| 8 | Prognosis of diabetics with diabetes onset before the age of thirty one显示文摘 | Deckert T Poulsen JE | 1978 | Diabetologia1978，14，： | 1 |
| 9 | The glycosylphosphatidylinositol-anchored CD59 protein stimulates both T cell receptor zeta/ZAP-70-dependent and -independent signaling pathways in T cells显示文摘 | Deckert M Ticchioni M Mari B | 1995 | Eur J Immunol1995，25，7： | 1 |
| 10 | Tip-enhanced Raman spectroscopy of single RNA strands:towards a novel direct-sequencing method显示文摘 | Bailo E Deckert V | | 0，，09： | 1 |
| 11 | U2 snRNA-protein contactsin purified human 17S U2 snRNPs and in spliceosomal A and B comple-xes显示文摘 | DYBKOV O WILL C L DECKERT J | 2006 | Molecular and Cellular Biology2006，26，7： | 1 |
| 12 | Cell wall investigations utilizing tip-enhanced Raman scattering显示文摘 | Budich C Neugebauer U Popp J Deckert V | | 0，，03： | 1 |
| 13 | Mammalian actin binding protein 1 is essential for endocytosis but not lamellipodia formation; functional analysis by RNA interference显示文摘 | Mise-Omata S Montagne B Deckert M | 2003 | Biochem Biophys Res Commun2003，301，3： | 1 |
| 14 | Central role of TGF-β in the pathogenesis of diabetic nephropathy and macro vascular complications显示文摘 | Deckert T | 1996 | Diabet Med1996，13，： | 1 |
| 15 | Specific tumour localisation of a huA33 antibody - carboxypeptidase A conjugate and activation of methotrexate - phenylalanine 显示文摘 | Deckert PM Bommann WG Ritter G | 2004 | Int J Oncol2004，24，5： | 1 |
| 16 | Resveratrol,a polypheonlic phytoalexin present in red wine,enhances expression and activity of endothelial nitric oxide synthase显示文摘 | Wallerath T Deckert G Ternes T Anderson H Li H Witte K | 2002 | Circulation2002，106，13： | 1 |
| 17 | Direct molecular-level near-field plasmon and temperature assessment in a single plasmonic hotspot显示文摘Tip-enhanced Raman spectroscopy(TERS)is currently widely recognized as an essential but still emergent technique for exploring the nanoscale.However,our lack of comprehension of crucial parameters still limits its potential as a user-friendly analytical tool.The tip’s surface plasmon resonance,heating due to near-field temperature rise,and spatial resolution are undoubtedly three challenging experimental parameters to unravel.However,they are also the most fundamentally relevant parameters to explore,because they ultimately influence the state of the investigated molecule and consequently the probed signal.Here we propose a straightforward and purely experimental method to access quantitative information of the plasmon resonance and near-field temperature experienced exclusively by the molecules directly contributing to the TERS signal.The detailed near-field optical response,both at the molecular level and as a function of time,is evaluated using standard TERS experimental equipment by simultaneously probing the Stokes and anti-Stokes spectral intensities.Self-assembled 16-mercaptohexadodecanoic acid monolayers covalently bond to an ultra-flat gold surface were used as a demonstrator.Observation of blinking lines in the spectra also provides crucial information on the lateral resolution and indication of atomic-scale thermally induced morphological changes of the tip during the experiment.This study provides access to unprecedented molecular-level information on physical parameters that crucially affect experiments under TERS conditions.The study thereby improves the usability of TERS in day-to-day operation.The obtained information is of central importance for any experimental plasmonic investigation and for the application of TERS in the field of nanoscale thermometry. | Marie Richard-Lacroix Volker Deckert | 2020 | Light(Science & Applications)2020，9，1： | 1 |
| 18 | Resveratrol, a polyphenolic phytoalexin present in red wine, enhances expression and activity of endothelial nitric oxide synthase 显示文摘 | Wallerath T Deckert G Ternes T | 2002 | Circulation2002，106，13： | 1 |
| 19 | Diagnosis and staging of Rasmussen's encephalitis by serial MRI and histopathology显示文摘 | Bien CG Urbach H Deckert M | | 0，，： | 1 |
| 20 | Cerebral sinus and venous thrombosis in rats induces long term deficits in brain function and morphology evidence for a cytotoxic genesis显示文摘 | Frerichs KU Deckert M Kempski O | 1994 | J Cerb Blood Flow Metab1994，14，2： | 1 |
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