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| 1 | Effect of fiber supplementation on the microbiota in critically ill patients显示文摘AIM:To determine tolerance to fiber supplementation of semi-elemental tube feeds in critically ill patients and measure its effect on colonic microbiota and fermentation.METHODS:Thirteen intensive care unit patients receiving jejunal feeding with a semi-elemental diet for predominantly necrotizing pancreatitis were studied.The study was divided into 2 parts:first,short-term (3-9 d)clinical tolerance and colonic fermentation as assessed by fecal short chain fatty acid(SCFA)concentrations and breath hydrogen and methane was measured in response to progressive fiber supplementation increasing from 4 g tid up to normal requirement levels of 8 g tid;second,4 patients with diarrhea were studied for 2-5 wk with maximal supplementation to additionally assess its influence on fecal microbiota quantitated by quantitative polymerase chain reaction (qPCR)of microbial 16S rRNA genes and Human Intestinal Tract Chip(HITChip)microarray analysis.Nearly all patients were receiving antibiotics(10/13)and acid suppressants(11/13)at some stage during the studies.RESULTS:In group 1,tolerance to progressive fiber supplementation was good with breath hydrogen and methane evidence(P=0.008 and P<0.0001,respectively)of increased fermentation with no exacerbation of abdominal symptoms and resolution of diarrhea in 2 of 4 patients.In group 2 before supplementation,fecal microbiota mass and their metabolites,SCFA,were dramatically lower in patients compared to healthy volunteers.From qPCR and HITChip analyses we calculated that there was a 97%reduction in the predominant potential butyrate producers and starch degraders.Following 2-5 wk of fiber supplementation there was a significant increase in fecal SCFA(acetate 28.4±4.1μmol/g to 42.5±3.1μmol/g dry weight,P=0.01;propionate 1.6±0.5 vs 6.22±1.1,P=0.006 and butyrate 2.5±0.6 vs 5.9±1.1,P=0.04)and microbial counts of specific butyrate producers,with resolution of diarrhea in 3 of 4 patients.CONCLUSION:Conventional management of critically ill patients,which includes the use of elemental diets and broad-spectrum antibiotics,was associated with gross suppression of the colonic microbiota and their production of essential colonic fuels,i.e.,SCFA.Our investigations show that fiber supplementation of the feeds has the potential to improve microbiota mass and function,thereby reducing the risks of diarrhea due to dysbiosis. | Stephen JD O'Keefe Junhai Ou James P DeLany Scott Curry Erwin Zoetendal H Rex Gaskins Scott Gunn | 2011 | World Journal of Gastrointestinal Pathophysiology2011，2，6： | 3 |
| 2 | Calculated optimal cooling rates for ram and human sperm cryopreservation fail to conform with empircal observations显示文摘 | Curry MR Millar JD Watson PF | 1994 | Biol Reprod1994，51，： | 1 |
| 3 | Nitrogen saturation in temperate forest ecosystems: Hypotheses revisited显示文摘 | Aber JD McDowell W Nadelhoffer K Magill A Bemtson G Kamakea M McNulty S Currie W Rustad L Fernandez I | 1998 | Bioscience1998，48，11： | 1 |
| 4 | Ecosystem response to 15 years of chronic nitrogen additions at the Harvard Forest LTER, Massachusetts, USA 显示文摘 | Magill AH Aber JD Currie WS NadelhoVer K J Martin ME McDo- well WH Melillo JM Steudler PA | 2004 | For Ecol Manage2004，196，： | 1 |
| 5 | Genetic susceptibilityto lymphoma显示文摘 | Skibola CF Curry JD Niceters A | 2007 | Haematologica2007，92，7： | 1 |
| 6 | Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1显示文摘 | Masson NM Currie IS Terrace JD | 2006 | Am J Physiol Gastrointest Liver Physiol2006，291，： | 1 |
| 7 | Obstacles to the implemen- tation of pharmaceutical care in the community setting 显示文摘 | Donough RP Rovers JP Currie JD | 1998 | J Am Pharm Assoc1998，38，1： | 1 |
| 8 | Effects of chronic nitrogen amendments on production of dissolved organic carbon and nitrogen in forest soils 显示文摘 | MCDOWELL WH CURRIE WS ABER JD YANO Y | 1998 | Water Air Soil Pollution1998，105，： | 1 |
| 9 | Modeling leaching as a decomposition pro- cess in humid montane forests显示文摘 | CURRIE WS ABER JD | 2007 | Ecology2007，78，： | 1 |
| 10 | Obstacles to the implementation of pharmaceutical care in the community setting 显示文摘 | McDonough RP Rovers JP Currie JD | 1998 | J Am Pharm Assoc1998，38，1： | 1 |
| 11 | Mechanical properties of mother of pearl in tension 显示文摘 | Curry JD | 1977 | Proc R Soc Lond1977，196，1125： | 1 |
| 12 | Genetic susceptibility to lymphoma显示文摘 | Skibola CF Curry JD Nieters A | 2007 | Haematologica2007，92，7： | 1 |
| 13 | Genetic susceptibility to lymphoma 显示文摘 | Skibola CF Curry JD Nieters A | 2007 | Haematologica2007，92，7： | 1 |
| 14 | Hepatic progenitor cells in human fetal liver express the oval cell marker Thy-1 显示文摘 | Masson NM Currie IS Terrace JD | 2006 | Am J Physiol Gastrointest Liver Physiol2006，291，1： | 1 |
| 15 | Vulvar cancer显示文摘 | NASH JD CURRY S | 1998 | Surg Oncol Clin N Am1998，7，： | 1 |
| 16 | Vertical transport of dissolved organic C and N under long-term N amendments in pine and hardwood forests显示文摘 | CURRIE WS ABER JD MCDOWELLWH | 1996 | Biogeochemistry1996，35，： | 1 |
| 17 | Vertical transport of dissolved organic C and N under long-term N amendments in pine and hardwood forests显示文摘 | Currie WS Aber JD McDowell WH | | 0，，： | 1 |
| 18 | Obstacles to the implementation of pharmaceutical care in the community setting显示文摘 | McDonough RP Rovers JP Currie JD | 1998 | J Am Pharm Assoc1998，38，1： | 1 |
| 19 | Hepatic progenitor cells in human fetal liver express the oval cellmarker Thy-1显示文摘 | Masson NM Currie IS Terrace JD | | 0，，01： | 1 |
| 20 | Vulvar cancer显示文摘 | Nash JD Curry S | 1998 | Surg Oncol Clin N Am1998，7，2： | 1 |
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