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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Management of mani{esta tions of epidermolysis bullosa显示文摘 | Elluru RG Contreras JM Albert DM | 2013 | Curr Opin Otolaryngol Head Neck Surg2013，21，6： | 1 |
| 3 | Sodium/potasium ATPase (Na^+ , K + -ATPase) and ouabain/related cardiac glyco- sides: a new paradigm for developnent of anti breast cancer drugs 显示文摘 | Chen JQ Contreras RG Wang R | 2006 | Breast Cancer lKes Treat2006，96，1： | 1 |
| 4 | The polarized expression of Na+,K+-ATPase in epithelia depends on the association between β-subunits located in neighboring cells显示文摘 | Shoshani L Contreras RG Roldan ML | 2005 | Mol Biol Cell2005，16，3： | 1 |
| 5 | Tight junction and polarity interaction in the transporting epithelial phenotype显示文摘 | Cereijido M Contreras RG Shoshani L | 2008 | Biochim Biophys Acta2008，1778，3： | 1 |
| 6 | Interaction of calcium with plasma membrane of epithelial (MDCK) cells during junction formation 显示文摘 | Contreras RG Miller JH Zamora M | 1992 | AmJPhysiol1992，263，21： | 1 |
| 7 | Tight junction and polarity interaction in the transporting epithelial phenotype显示文摘 | Cereijido M Contreras RG Shoshani L | 2008 | Biochim Biophys Acta2008，1778，3： | 1 |
| 8 | Type of cell death induced by various metal cations in cultured human gingival fibroblasts显示文摘 | Contreras RG Sakagami H Nakajima H | 2010 | In Vivo2010，24，4： | 1 |
| 9 | Epithelial tight junctions显示文摘 | Cereijido M Gonzalez-Mariscal L Contreras RG | 1988 | Am Rev Respir Dis1988，138，62： | 1 |
| 10 | Sodium/potassium ATPase (Na + ,K+-ATPase)and ouabain/related cardiac glycosides:A new paradigm for development of anti- breast cancer drugs? 显示文摘 | Chen JQ Contreras RG Wang R | 2006 | Breast Cancer Res Treat2006，96，1： | 1 |
| 11 | Cell adhesion, polarity, and epithelia in the dawn of metazoans显示文摘 | Cereijido M Contreras RG Shoshani L | 2004 | Physiol Rev2004，84，4： | 1 |
| 12 | The polarized expression of Na^+,K^+ -ATPase in epithelia depends on the association between beta-subunits located in neighboring cells 显示文摘 | Shoshani L Contreras RG Roldan ML | 2005 | Mol Biol Cell2005，16，3： | 1 |
| 13 | Sodium/potasium ATPase (Na(^+),K (^+)-ATPase) and ouabain/related cardiac glycosides:a new paradigm for development of anti-breast cancer drugs?显示文摘 | Chen JQ Contreras RG Wang R | 2006 | Breast Cancer Res Treat2006，96，1： | 1 |
| 14 | The polarized expression of Na^+,K^+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells显示文摘 | Shoshani L Contreras RG Roldan ML | 2005 | Mol Biol Cell2005，16，3： | 1 |
| 15 | The polarized expression of Na+, K +-ATPase in epithelia depends on the association between beta-subunits located in neighboring ceils 显示文摘 | Shoshani L Contreras RG Roldan ML | 2005 | Mol Biol Cell2005，16，3： | 1 |
| 16 | The polarized expression of Na+,K+-ATPase in epithelia depends on the association between beta-subunits located in neighboring cells显示文摘 | Shoshani L Contreras RG Roldan ML | 2005 | Mol Biol Cell2005，16，3： | 1 |
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