| 1 | A novel ubiquitin carboxyl terminal hydroase is involved in toad oocyte maturation显示文摘p28, a 28kD protein from toad (Bufo bufo gargarizans) oocytes, was identified by using p13suc1-agaroseaffinity chromatography. Sequence homology analysis of the full-length cDNA of p28 (Gene Bank accessionnumber: AF 314091) indicated that it encodes a protein containing 224 amino-acids with about 55% iden-tities and more than 70% positives to human, rat or mouse UCH-L1, and contains homological functionaldomains of UCH family. Anti-p28 monoclonal antibody, on injecting into the oocytes, could inhibit theprogesterone-induced resumption of meiotic division in a dose-dependent manner. The recombinant proteinp28 showed similar SDS/PAGE behaviors to the native one, and promoted ubiquitin ethyl ester hydrolysis,a classical catalytic reaction for ubiquitin carboxyl terminai hydrolases (UCHs). The results in this paperreveal that a novel protein, p28, exists in the toad oocytes, is a UCH L1 homolog, was engaged in theprocess of progesterone-induced oocyte maturation possibly through an involvement in protein turnover anddegradation. | ZHAO GUI SUN1,2,3,*, WEI HUA KONG2,*, YAN JUN ZHANG2, SHAN YAN1, JI NING LU1, ZHENG GU1, FENG LIN1, JIA KE TSO1,**1 National Laboratory of Contraceptives and Devices Research, Shanghai Institute of Planned ParenthoodResearch, Shanghai 200032, China2 Colle | 2002 | Cell Research2002，12，4： | 8 |
| 2 | Chromosome location of semidwarf gene sd-t and sd-n in indica rice显示文摘Great achievements have been madein breeding of semidwarf varieties(indica) since 1960e. Resultsshowed that the dwarf traits widelyused at present were controlled by thesame recessive major gene, sd-1,thus we faced the potential danger oflosing genetic diversity. The low yieldof semidwarf varieties newly-bred indomestic or abroad may be related tothe narrow use of dwarf resource. Soit was important to explore, appraise, | LI Xin~((1))) Gu Minghong~(1)) Xu Jin-feng~(2)) YAN Changjie~(1)) LIANG Guohua~(1)) 1)Dept of Agronomy,Agri Coll,Yangzhou Univ ,Yangzhou 225009 2)Office of Guannan County Goverment,Guannan 222500,China | 2002 | Chinese Rice Research Newsletter2002，10，1： | 1 |
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