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2篇 您的检索式:作者名="Claudio Forte"
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1Dietary supplementation of Rosmarinus officinalis L.leaves in sheep affects the abundance of rumen methanogens and other microbial populations显示文摘Background: Rumen microbiome has a great influence on ruminant health and productivity. Different plant extracts have been tested for their ability to modulate the rumen microbiome to improve feed digestion and fermentation. Among the evaluated plant extracts, essential oils, tannins, and saponins appeared to have positive effects on rumen protein metabolism, volatile fatty acids production, and methane and ammonia production.Methods: The objective of this study was to evaluate the effect of rosemary(Rosmarinus officinalis L.) leaves and essential oils on rumen microbial populations. Four ruminally cannulated sheep were used in a 4×4 Latin square design fed(21 d/period): 1) a control diet composed of alfalfa hay and concentrate pellet(CTR), 2) CTR supplemented with 7 g/d/sheep of rosemary essential oil adsorbed on an inert support(EO), 3) CTR with 10 g/d/sheep of dried and ground rosemary leaves(RL), and 4) CTR with 10 g/d of dried and ground rosemary leaves pelleted into concentrate(RL pellet). Abundance of total bacteria, archaea, protozoa, and some select bacterial species or groups was quantified using q PCR, while the community of bacteria and archaea was profiled using denaturing gradient gel electrophoresis.Results: No difference in abundance was noted for total bacteria, protozoa, or Ruminococcus flavefaciens between the control and the treatments, but the rosemary leaves, either in loose form or in pellet, decreased the abundance of archaea and the genus Prevotella(P < 0.001). The rosemary leaves in loose form also decreased(P < 0.001) the abundance of Ruminococcus albus and Clostridium aminophilum, while the EO increased(P < 0.001) the abundance of Fibrobacter succinogenes. The community of bacteria and archaea was not affected by any of the supplements.Conclusions: Being able to affect the abundance of several groups of rumen microbes that are known to be involved in degradation of protein and fiber and production of methane and ammonia, rosemary leaves may be used to modulate rumen microbiome and its function.Gabriella Cobellis Zhongtang Yu Claudio Forte Gabriele Acuti Massimo Trabalza-Marinucci 2016Journal of Animal Science and Biotechnology2016,7,4:3
2Non-exhaustive double effort test is reliable and estimates the firs ventilatory threshold intensity in running exercise显示文摘Purpose: The present study aimed to investigate the reliability of the non-exhaustive double effort(NEDE) test in running exercise and its associations with the ventilatory thresholds(VT_1 and VT_2) and the maximal lactate steady state(MLSS).Methods: Ten healthy male adults(age: 23 ± 4 years, height: 176.6 ± 6.4 cm, body mass: 76.6 ± 10.7 kg) performed 4 procedures:(1) a ramp test for VT_1 and VT_2 determinations measured by ratio of expired ventilation to O_2 uptake(VE/VO_2) and expired ventilation to CO_2 output(VE/VCO_2) equivalents, respectively;(2) the NEDE test measured by blood lactate concentration(NEDE_(LAC)) and heart rate responses(NEDE_(HR));(3) a retest of NEDE for reliability analysis; and(4) continuous efforts to determine the MLSS intensity. The NEDE test consisted of4 sessions at different running intensities. Each session was characterized by double efforts at the same running velocity(E1 and E2, 180 s), separated by a passive recovery period(90 s rest). LAC and HR values after E1 and E2(in 4 sessions) were used to estimate the intensity equivalent to'null delta' by linear fit. This parameter represents, theoretically, the intensity equivalent to maximal aerobic capacity.Results: The intraclass correlation coefficient indicated significant reliability for NEDE_(LAC)(0.93) and NEDE_(HR)(0.79)(both p < 0.05). There were significant correlations, no differences, and strong agreement with the intensities predicted by NEDE_(LAC)(10.1 ± 1.9 km/h) and NEDE_(HR)(9.8 ± 2.0 km/h) to VT_1(10.2 ± 1.1 km/h). In addition, despite significantly lower MLSS intensity(12.2 ± 1.2 km/h), NEDE_(LAC) and NEDE_(HR) intensities were highly correlated with this parameter(0.90 and 0.88, respectively).Conclusion: The NEDE test applied to running exercise is reliable and estimates the VT_1 intensity. Additionally, NEDE intensities were lower but still correlated with VT_2 and MLSS.Lucas Dantas Maia Forte Fúlvia Barros Manchado-Gobatto Roberta Cunha Matheus Rodrigues Maria Ceclia Gallani Claudio Alexandre Gobatto 2018Journal of Sport and Health Science2018,7,2:0
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