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| 1 | Isolation and characterization of glacier VMY22, a novel lytic cold-active bacteriophage of Bacillus cereus显示文摘As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a 'living fossil' for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head(59.2 nm in length, 31.9 nm in width) and a tail(43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at p H 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study. | Xiuling Ji Chunjing Zhang Yuan Fang Qi Zhang Lianbing Lin Bing Tang Yunlin Wei | 2015 | Virologica Sinica2015，30，1： | 5 |
| 2 | MS1 is essential for male fertility by regulating the microsporocyte cell plate expansion in soybean显示文摘Male sterility is an essential trait in hybrid seed production,especially for monoclinous and autogamous food crops.Soybean male-sterile ms1 mutant has been known for more than 50 years and could be instrumental in making hybrid seeds.However,the gene responsible for the male-sterile phenotype has remained unknown.Here,we report the map-based cloning and characterization of the MS1 gene in soybean.MS1 encodes a kinesin protein and localizes to the nucleus,where it is required for the male meiotic cytokinesis after telophase Ⅱ.We further substantiated that MS1 colocalizes with microtubules and is essential for cell plate formation in soybean male gametogenesis through immunostaining.Both ms1 and CRISPR/Cas9 knockout mutants show complete male sterility but are otherwise phenotypically normal,making them perfect tools for producing hybrid seeds.The identification of MS1 has the practical potential for assembling the sterility system and speeding up hybrid soybean breeding. | Xiaolong Fang Xiaoyuan Sun Xiangdong Yang Qing Li Chunjing Lin Jie Xu Wenjun Gong Yifan Wang Lu Liu Limei Zhao Baohui Liu Jun Qin Mengchen Zhang Chunbao Zhang Fanjiang Kong Meina Li | 2021 | Science China(Life Sciences)2021，64，9： | 4 |
| 3 | Compositions and Pressure-Temperature Conditions of Metamorphic Fluids Overprinting the Talate VMS Pb-Zn Deposit,Southern Altay,China显示文摘Talate Pb-Zn 存款,在扩大泥盆纪 Kelan 的 NW-SE 的东方定位了南部的 Altaides 的暴烈沉积的盆,发生在更低的泥盆纪更低的 Kangbutiebao 形成的上面的秒 lithological 节的变形岩石系列(D 1 k 12) 。Pb-Zn orebodies 由迟了的硫化物石英静脉层状、套印。二个不同矿化作用时期被识别:一个海底的暴烈的沉积呼气时期和一个变形热水的矿化作用时期。变形套印时期能进一步被划分成二个阶段:透镜状的石英静脉在绿泥石片岩和忍受矿石的地平线的 leptite 开发了的床上用品平行描绘的一个早阶段,和一个迟了的阶段由横切绿泥石片岩和 leptite 或巨大的 Pb-Zn 矿石的 pyrite-chalcopyrite-quartz 静脉代表了。在早变形的石英静脉的液体包括主要是公司 2-H2 O-NaCl 并且碳(有次要的水的包括的公司 2±CH4±N2) 包括。公司 2-H2 O-NaCl 包括有 294-368 ° C 的 homogenization 温度, T m,到 -60.5°C, T > h, 7.7 ~ 29.6 °C 的 CO2 ( 一致进液体) ,并且 5.5-7.4 wt% NaCl eqv 的咸度。碳的包括有 T m,到 -58.5°C, 和 T > h,到 20.6 °C 的 -4.2 的 CO2 。在迟了的硫化物石英静脉的液体包括被公司 2-H2 O-NaCl 和公司 2±CH4 包括。公司 2-H2 O-NaCl 包括有 T h, 142 ~ 360 °C 的小孩 , T m,到 -56.6°C, T > h,到 29.4 °C 的 -6.0 的 CO2 ( 一致进液体) 并且 2.4-16.5 wt% NaCl eqv 的咸度。碳的包括有 T m,到 -57.3°C, 和 T > h,到 28.7 °C 的 -27.0 的 CO2 。水的包括(L-V ) 有 T m,到 -1.3°C 和 T > h, 205 ~ 412 °C 的小孩 。套住公司 2-rich 液体包括的条件的磅(100-370 MPa, 250-368 °C ) 是可比较的与迟了 -- 到 regional 以后变态条件。公司 2-rich 液体,可能源于地区性的变态,涉及重做和主要矿石的金属丰富。把结果基于这些, Talate Pb-Zn 存款作为变形液体修改的 VMS 存款被分类。有 banded 和角砾岩结构的巨大的 Pb-Zn 矿石在与 extensional 联系的海底的暴烈的沉积呼气的早时期被开发 subduction 相关的背弧盆,和忍受 polymetallic 硫化物的石英静脉在与二叠三叠纪的大陆人碰撞有关的变形热水的重迭的迟了的时期被形成。 | ZHANG Hui XU Jiuhua CHENG Xihui GUO Xuji LIN Longhua YANG Rui BIAN Chunjing | 2015 | Acta Geologica Sinica(English Edition)2015，89，3： | 2 |
| 4 | Hepatitis B e Antigen Seroconversion Is Related with the Function of Dendritic Cells in Chronic Hepatitis B Virus Infection显示文摘 | Chunjing Lin Hai Zou Shumin Wang Paolo Gionchetti | 2014 | Gastroenterology Research and Practice2014，，： | 1 |
| 5 | lncRNA-AC130710 targeting by miR-129-5p is upregulated in gastric cancer and associates with poor prognosis显示文摘 | Chunjing Xu Yongfu Shao Tian Xia Yunben Yang Jiawei Dai Lin Luo Xinjun Zhang Weiliang Sun Haojun Song Bingxiu Xiao Junming Guo | 2014 | Tumor Biology2014，，10： | 1 |
| 6 | MALE STERILITY 3 encodes a plant homeodomain-finger protein for male fertility in soybean显示文摘Male-sterile plants are used in hybrid breeding to improve yield in soybean(Glycine max(L.)Merr.).Developing the capability to alter fertility under different environmental conditions could broaden germplasm resources and simplify hybrid production.However,molecular mechanisms potentially underlying such a system in soybean were unclear.Here,using positional cloning,we identified a gene,MALE STERILITY 3(MS3),which encodes a nuclear-localized protein containing a plant homeodomain(PHD)-finger domain.A spontaneous mutation in ms3 causing premature termination of MS3 translation and partial loss of the PHD-finger.Transgenetic analysis indicated that MS3 knockout resulted in nonfunctional pollen and no selfpollinated pods,and RNA-seq analysis revealed that MS3 affects the expression of genes associated with carbohydrate metabolism.Strikingly,the fertility of mutant ms3 can restore under longd conditions.The mutant could thus be used to create a new,more stable photoperiod-sensitive genic male sterility line for two-line hybrid seed production,with significant impact on hybrid breeding and production. | Jingjing Hou Weiwei Fan Ruirui Ma Bing Li Zhihui Yuan Wenxuan Huang Yueying Wu Quan Hu Chunjing Lin Xingqi Zhao Bao Peng Limei Zhao Chunbao Zhang Lianjun Sun | 2022 | Journal of Integrative Plant Biology2022，64，5： | 1 |
| 7 | A Novel Secure Cosine Similarity Computation Scheme With Malicious Adversaries显示文摘 | De Xin Yang ChunJing Lin Bo Yang | 2013 | International Journal of Network Security & Its Applications(IJNSA)2013，5，02： | 1 |
| 8 | Customer-driven Product Configuration Optimization for Assemble-to-order Manufacturing Enterprises 显示文摘 | Chunjing Zhou Zhihang Lin Chuntao Liu | 2007 | The International Journal of Advanced Manufacturing Technology2007，38，12： | 1 |
| 9 | Experiment and simulation of a LiFePO4 battery pack with a passive thermal management system using composite phase change material and graphite sheets显示文摘 | LIN Chunjing XU Sichuan CHANG Guofeng | 2015 | Journal of Power Sources2015，275，： | 1 |
| 10 | Morphological diversity of cultured cold-active lytic bacteriophages isolated from the Napahai plateau wetland in China显示文摘Dear Editor,Viruses are the most abundant,diverse,and ubiquitous entities(approximately 1031)on Earth.They play major roles in horizontal gene transfer,the regulation of bacterial community structures,as well as nutrient and energy cycles of marine ecosystems(Danovaro et al.,2008).In particular,lytic bacteriophages(phages)can infect and kill bacteria without harming human or | Xiuling Ji Chunjing Zhang Anxiu Kuang Jiankai Li Yinshan Cui Kunhao Qin Lianbing Lin Benxu Cheng Qi Zhang Yunlin Wei | 2015 | Virologica Sinica2015，30，6： | 0 |
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