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7篇 您的检索式:作者名="Chris Lowe"
    题名 作者 年代 出处 被引量
1A framework for the governance of social enterprise显示文摘Chris Low 2006International Journal of Social Economics2006,,5:2
2An XPS and ToF-SIMS investigation of the outermost nanometres of a poly (vinyiidene difluoride) coating 显示文摘Steven J Hinder Chris Lowe John F Watts 2007Progress in Organic Coatings2007,,60:1
3Confocal Raman microscopy study of the melamine distribution in polyester–melamine coil coating显示文摘Wanrui Zhang Ray Smith Chris Lowe 2009Journal of Coatings Technology and Research2009,,3:1
4Urine exosome mRNA-based test for monitoring kidney allograft rejection:Effects of sample transportation and storage,and interference substances显示文摘BACKGROUND Exosomes are 30-150 nm nanovesicles with sophisticated nucleic acids cargo,actively secreted by all cells within human body,and found in abundance in all body fluids,including urine.These extracellular vesicles have tremendous potential for next generation diagnostics,theoretically enabling noninvasive assessment of organ and tissue function via liquid biopsy analysis.AIM Recently,feasibility of an exosomal molecular test was demonstrated for postorgan transplant monitoring:Analysis of urine-derived exosomal mRNA cargo allowed early detection of kidney allograft rejection.Here,we further studied urine-derived exosomes and their mRNA content as a highly promising diagnostic modality.This included stability studies of urine samples and exosomal mRNA upon transportation from the point of collection to a centralized testing facility,short-term storage of urine at different conditions upon receipt till the point molecular assay is performed,and effects of various potentially interfering substances on the downstream quantitative polymerase chain reaction(qPCR)assay.METHODS The urine specimens were stored at various conditions and pre-processed in different ways.Next,samples were passed through the columns to capture all extracellular vesicles,the vesicles were lysed to release their content and the exosomal RNA was purified on the mini-columns,reverse transcription was performed,next pre-amplification,followed by a qPCR analysis for a panel of RESULTS To ensure exosomal RNA integrity,the harvested urine specimens should be shipped refrigerated,by overnight delivery.Urine can next be stored at the test site for up to 1 wk at 4°C,and long term should be frozen at-80°C.Urine specimens must be centrifuge at low G-force to deplete cells and debris,to ensure consistent top results in downstream molecular assays.All commonly used medications(tacrolimus,cyclosporin A,mycophenolic acid,everolimus,sirolimus,ascomycin,teriflunomide)were tested and confirmed that they do not cause assay interference.CONCLUSION mRNA from urine-derived exosomes was shown to be stable across a broad range of conditions and produced accurate results when analyzed via qPCR assay for detection of kidney allograft rejection.We identified the most optimal conditions for every step of the process,ensuring pre-analytical sample integrity and robust qPCR results.Matt McFaul Chris Ventura Sean Evans Halil Dundar Marc J Rumpler Christopher McCloskey Dave Lowe Alexandre V Vlassov 2023World Journal of Methodology2023,13,5:0
5被难倒的“大侦探”显示文摘丹尼尔非常擅长推理,他帮助别人解决了许多难题,因此被誉为“四年级最棒的侦探”。一次,珍妮告诉丹尼尔,他们的同学科比在周一一大早被接走后就再也没有来过学校。珍妮想知道科比去哪儿了。随着目击证人提供各种线索,丹尼尔也一步步接近事实真相。然而在这时,他突然决定停止这次调查任务。Chris Low Michelle Simpson 2022英语沙龙(原版阅读)2022,,5:0
6Estimation of premature forests in Georgia (USA) using U.S.Forest Service FIA data and Landsat imagery显示文摘We used geographic information system applications and statistical analyses to classify young, premature forest areas in southeastern Georgia using combined data from Landsat TM 5 satellite imagery and ground inventory data. We defined premature stands as forests with trees up to 15 years old. We estimated the premature forest areas using three methods: maximum likelihood classification(MLC), regression analysis, and k-nearest neighbor(k NN)modeling. Overall accuracy(OA) of classifying the premature forest using MLC was 82% and the Kappa coefficient of agreement was 0.63, which was the highest among the methods that we have tested. The k NN approach ranked second in accuracy with OA of 61% and a Kappa coefficient of agreement of 0.22. Regression analysis yielded an OA of 57% and a Kappa coefficient of 0.14. We conclude that Landsat imagery can be effectively used for estimating premature forest areas in combination with image processing classifiers such as MLC.Hojung Kim Chris J. Cieszewski Roger C. Lowe 2017Journal of Forestry Research2017,28,6:0
7照亮2008欧洲文化之都——英国利物浦天堂街开发区照明规划显示文摘在利物浦市中心.一片新区正在建设中:这就是天堂街开发区(Paradise Street Development Area)。此开发区位于一些具有历史文化价值的城市空间和构筑物的怀抱中.四周是主要的交通干线。开发方案包括一个住宅区,还有宾馆、商业和体闲区等。此种规模的城市规划项目需要城市规划师和建筑师通力合作,以取得最佳效果,同时,对于展示专业照明设计师如何向城市设计团队提供支持而言,这也是个绝佳的机会。BDP照明团队的任务就是从这个项目的总体规划着手。将新开发区和现有城市格局融为一体.并为建筑物.街道和步行区提供照明,以达到令人耳目一新的效果。Laura Bayliss Chris Lowe Kai Becker 李彩歌(译) 2007照明设计2007,,1:0
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