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| 1 | Endostatin binds to blood vessels in situ independent of heparin sulfate and does not compete for tlbroblast growth factor-2 binding显示文摘 | Chang Z Choon A Friedl A | 1999 | Am J Pathol1999，155，1： | 1 |
| 2 | Endostatin binds to biood veaaels in situ independent of heparan sulfaet and does not compete for fibroblast growth factor-2 binding显示文摘 | Chang Z Choon A Friedl A | 1999 | Am J Pathol1999，155，： | 1 |
| 3 | Endostatin binds to blood vessels in situ independent of heparan sulfate and dose not compete for FGF-2 binding显示文摘 | Choon A Friedl A | 1999 | Am J Pathol1999，155，1： | 1 |
| 4 | The impact of XBRLadoption in PR China 显示文摘 | Chunhui Li u Xi n Luo Choon Li ng Si a Gra ceO'Farrell Hock Hai Teo | 2014 | Decision Support Systems2014，59，3： | 1 |
| 5 | Relational archetypes, organizational learning, and value creation: Extending the human resource architecture 显示文摘 | Sung- Choon Kang Morris Shad S Snell Scott A | 2007 | Academy of Management Review2007，32，1： | 1 |
| 6 | New Pool Boiling Data for Water With Copper-Foam Metal at Sub- Atmospheric Pressures: Experiments and Correlation 显示文摘 | Choon N K Chakraborty A Aye S M | 2006 | Applied Thermal Engineering2006，26，： | 1 |
| 7 | Interaction of cells andnanofiber scaffolds in tissue engineering 显示文摘 | Venugopal J Low S Choon A T | 2008 | Journal of BiomedicalMaterials Research Part B: Applied Biomaterials2008，84，： | 1 |
| 8 | Generation of a human embryonic stem cell line stably expressing high levels of the fluorescent protein mCherry显示文摘AIM:The generation and characterization of a human embryonic stem cell (hESC) line stably expressing red fluorescent mCherry protein. METHODS:Lentiviral transduction of a ubiquitously-expressed human EF-1α promoter driven mCherry transgene was performed in MEL2 hESC. Red fluore-scence was assessed by immunofluorescence and flow cytometry. Pluripotency of stably transduced hESC was determined by immunofluorescent pluripotency marker expression, flow cytometry, teratoma assays andembryoid body-based differentiation followed by reverse transcriptase-polymerase chain reaction. Quantification of cell motility and survival was performed with time lapse microscopy. RESULTS:Constitutively fluorescently-labeled hESCs are useful tools for facile in vitro and in vivo tracking of survival, motility and cell spreading on various surfaces before and after differentiation. Here we describe the generation and characterization of a hESC line (MEL2) stably expressing red fluorescent protein, mCherry. This line was generated by random integration of a fluorescent protein-expressing cassette, driven by the ubiquitously-expressed human EF-1α promoter. Stably transfected MEL2-mCherry hESC were shown to express pluripo-tency markers in the nucleus (POU5F1/OCT4, NANOG and SOX2) and on the cell surface (SSEA4, TRA1-60 and TG30/CD9) and were shown to maintain a normal karyotype in long-term (for at least 35 passages) culture. MEL2-mCherry hESC further readily differentiated into representative cell types of the three germ layers in embryoid body and teratoma based assays and, importantly, maintained robust mCherry expression throughout differentiation. The cell line was next adapted to singlecell passaging, rendering it compatible with numerous bioengineering applications such as measurement of cell motility and cell spreading on various protein modified surfaces, quantification of cell attachment to nanoparticles and rapid estimation of cell survival. CONCLUSION:The MEL2-mCherry hESC line conforms to the criteria of bona fide pluripotent stem cells and maintains red fluorescence throughout differentiation, making it a useful tool for bioengineering and in vivo tracking experiments. | Dmitry A Ovchinnikov Jennifer P Turner Drew M Titmarsh Nilay Y Thakar Dong Choon Sin Justin J Cooper-White Ernst J Wolvetang | 2012 | World Journal of Stem Cells2012，4，7： | 1 |
| 9 | Endostatin binds to blood veaaels in situ independent of heparan sulfaet and does not compete for fibroblast growth factor - 2 binding显示文摘 | Chang Z Choon A Ffiedl A | 1999 | Am J Pathol1999，155，： | 1 |
| 10 | New pool boiling data for water with copper-foam metal at sub-atmos- pheric pressure : experiment and correlation 显示文摘 | Choon N K Chakraborty A Aye S M | 2006 | Applied Thermal Engineering2006，26，1112： | 1 |
| 11 | Prostate-specific antigen,testosterone,sex-hormone binding globulin and androgen receptor CAG repeat polymorphisms in subfertile and normal men显示文摘 | Mifsud A Choon A T Fang D | 2001 | Mol Hum Reprod2001，7，11： | 1 |
| 12 | Endostatin binds to biood veaaels in situ independent of hoparan sulfael and does not compete for fibroblast growth factor-2 binding显示文摘 | Chang Z Choon A Friedl A | 1999 | Am J Pathol1999，155，： | 1 |
| 13 | Endostatin binds to blood vessels in situ independent of heparan sulfate and does not compete for fibroblast growth factor - 2 binding显示文摘 | Chang Z Choon A and Friedl A | 1999 | Am J Pathol1999，155，： | 1 |
| 14 | Solar- assisted dual-effect adsorption cycle for the production of cooling effect and potable water显示文摘 | Kim Choon Ng Kyaw Thu Chakraborty A | 2009 | International Journal of Low-Carbon Technologies Advance Access2009，3，： | 1 |
| 15 | How heat and mass recovery strategies impact the performance of adsorption desalination plant: theory and experiments 显示文摘 | Wang Xiaolin Chakraborty A Kim Choon Ng | 2007 | Heat Transfer Engineering2007，28，2： | 1 |
| 16 | Endostatin binds to blood vessels in situ independent of heparan sulfate and does not compete for fibro- blast growh factor-2 binding显示文摘 | Chang Z Choon A Friedl A | 1999 | Am J Pathol1999，155，1： | 1 |
| 17 | Passive learning and input-to-state stability of switched Hopfield neural networks with time-delay 显示文摘 | Choon K A | 2010 | Information Sciences2010，180，： | 1 |
| 18 | Min- eralization of osteoblasts with electrospun collagen/ hydroxyapatite nanofibers 显示文摘 | VENUGOPAL J LOW S CHOON A T | 2008 | Journal of Materials Sci- ence : Materials in Medicine2008，19，5： | 1 |
| 19 | A protocol for improving fabrication yield of thin SU-8 microcantilevers for use in an aptasensor显示文摘 | CHOON LIM Y KOUZANI A Z KAYNAK A | 2015 | Microsystem Technologies2015，21，2： | 1 |
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