| 2 | Gene-like Precise Construction of Functional DNA Materials显示文摘CONSPECTUS:Developing a new system of material chemistry is an important molecular foundation for the design and preparation of functional materials,which resembles the preparation of raw materials such as bricks for constructing a building that determines the quality of the building.However,precisely controlling the synthesis processes and structures of functional materials always remains a grand challenge.It is expected to propose a paradigm of“gene-like”precise construction to realize the rational synthesis of functional materials,i.e.,a“structure-function-application”principle for the precise construction.As the core genetic material of life,the DNA molecule is a bioactive macromolecule that can accurately encode genetic information and also can act as the generic molecular building block for the precise construction of functional materials.In this Account,we describe our work on the design and construction of functional DNA materials,to illustrate the principle of“gene-like”construction of functional DNA materials.The DNA molecule shows the unique advantages in the“gene-like”construction of functional materials,mainly including the precise arrangement of bases(monomers),the controllable design and assembly of structure,the precise transmission of sequence information,and customization of function.First,the number and sequence of four deoxynucleotide monomers that constitute the DNA strand can be rationally designed and accurately synthesized.Second,the sequence information on DNA endows the precise and efficient assembly of DNA molecules and ensures the precise regulation on the specific biological functions of functional DNA materials.Third,the functions of DNA materials can couple with the biological environments,so as to achieve the predetermined applications.Based on the scale of the constructed DNA materials,we categorize DNA materials into three classes:molecular scale,nanoscale,and macroscale.At the molecular scale,the representative material is branched DNA-based materials;the construction strategies mainly include targettriggered polymerization,enzymatic extension,and hybrid coupling;the applications mainly include the nonenzymatic detection of base mutation,the construction of artificial cells for the study of compartmentalization and the confinement effect,and the regulation of optical and antibacterial properties of supernanoclusters.At the nanoscale,the representative material is the DNA nanocomplex;the construction strategies mainly include hybridization with polymer,small molecule,and metal ions;the applications mainly include gene drug delivery and luminescence bioimaging.At the macroscale,the representative material is the DNA hydrogel;the construction strategies mainly include double-rolling circle amplification(double-RCA),multistage-RCA,and chemical cross-linking;the applications mainly include cell isolation,cell delivery,antibacterial agents,and self-healing electric circuits.Based on the“gene-like”paradigm,we expect to develop a wider variety of functional DNA materials by the precise regulation of DNA assembly behaviors and topological structures.We further envision that our work on the design,synthesis,and applications of functional DNA materials is a typical paradigm for the“gene-like”precise construction of functional materials and hopefully will promote the development of materials genome. | Chi Yao Yuwei Xu Pin Hu Junhan Ou Dayong Yang | 2022 | Accounts of Materials Research2022，3，1： | 0 |
| 3 | The Application of a Safe Neutralization Assay for Ebola Virus Using Lentivirus-Based Pseudotyped Virus显示文摘Dear Editor,Zaire Ebola virus(EBOV)is a negative-sense singlestranded RNA virus that is capable of causing acute hemorrhagic fever with a frightening fatality rate that can reach up to 90%.Due to its high lethality and frequent recurrence,EBOV is a substantial threat to public health.Research about live EBOV is restricted to BSL-4 laboratories;therefore,conventional serological detection methods do not provide a safe way to evaluate neutralizing activity,resulting in a bottleneck in antibody-based detection of viruses such as EBOV.Here,we aim to develop and apply a neutralization assay for EBOV using a generated lentivirus-based pseudotyped EBOV bearing GP on its surface. | Zengguo Cao Hongli Jin Gary Wong Ying Zhang Cuicui Jiao Na Feng Fangfang Wu Shengnan Xu Hang Chi Yongkun Zhao Tiecheng Wang Weiyang Sun Yuwei Gao Songtao Yang Xianzhu Xia Hualei Wang | 2021 | Virologica Sinica2021，36，6： | 0 |
| 4 | Construction and applications of DNA-based nanomaterials in cancer therapy显示文摘As a biologically active macromolecule, deoxyribonucleic acid(DNA) has the advantages of sequence programmability and structure controllability and can accurately transmit sequence information to specific biological functions. Facing the complex internal microenvironment and heterogeneity in tumor treatment, the construction and applications of DNA-based nanomaterials have become a focus point of research. In particular, the hybridization of DNA molecules with other materials endows DNA-based nanomaterials with multiple functions such as targeting, stimulus responsiveness and regulations of biological activities, making DNA nanostructures great potential in the treatment of major human diseases.In this review, the construction and characteristics of DNA-based nanomaterials are introduced. Then,the functions and applications of DNA-based nanomaterials in the delivery of chemotherapy drugs and gene drugs, stimulus-responsive release and regulation of cell homeostasis are reviewed. Finally, the future development and challenges of DNA-based nanomaterials are prospected. We envision that DNAbased nanomaterials can enrich the nanomaterial system by rational design and synthesis and address the growing demands on biological and biomedical applications in the real world. | Hedong Qi Yuwei Xu Pin Hu Chi Yao Dayong Yang | 2022 | Chinese Chemical Letters2022，33，3： | 0 |
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