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| 1 | Regional disparity in the changes of agricultural land use intensity in China during 1980-2002显示文摘Based on the cost-benefit data (1980–2002) of farm products and China Agriculture Yearbooks, this paper studies the regional disparity in the changes of the agricultural land use in China during the period 1980–2002 from three aspects such as the degree of intensity, the sown area and the abandoned farmland. The results show that: (1) The degree of intensity of land use in the western region during 1980–2000 has a strong uptrend, but in the eastern and central regions the degree of intensity descends obviously and has shown a continuous downtrend since 1997. (2) The total sown area shrinks notably in the eastern region, while it enlarges constantly in the western region. (3) The sown area in the eastern, central and western regions has gone through a similar cyclic process: down (1980–1985)–up (1985–1991)–down (1991–1994)–up (1994–1999)–down (1999–2002). However, there are obvious differences in amplitude variation and tendency among them. The sown area has shrunk in the eastern region and expanded in the central and western regions especially before 1999. (4) The most cases of abandoned farmland are reported in the central region, the second in the eastern region and the least in the western region. The abandonment phenomena chiefly occurred during 1992–1995 in the eastern region, and during 1998–2002 in the central region. | LIU Chengwu LI Xiubin | 2006 | Journal of Geographical Sciences2006，16，3： | 16 |
| 2 | An experimental study exploring the influencing factors for ultrasonic-assisted extraction of flavonoid compounds from leaves of Amorpha fruticosa L.显示文摘In this paper,the ultrasonic-assisted extraction process of flavonoid compounds from leaves of Amorpha fruticosa is optimized.In single factor experiments,solid/liquid ratios,ultrasonic power,ethanol concentrations and extraction cycles were experimental factors.Box–Behnken central composite design and RSM analyzed the effects of the four factors on the yield of total flavonoids.The optimal extraction parameters were solid/liquid ratio 1:50 g/mL,ultrasonic power 316 W,ethanol concentration 50%,4 extraction cycles.In the optimized condition,the estimated value of the regression model was 66.6456 mg/g while the measured value was 66.4329 mg/g. | Jingyao Tian Siddique Muhammad Ai Chen Peng Chen Jinghong Wang Chengwu Yang Hui Yuan Zhensheng Wang | 2019 | Journal of Forestry Research2019，30，5： | 14 |
| 3 | Coal and gas outburst mechanism of the “Three Soft” coal seam in western Henan显示文摘Based on the particularities of gas outbursts,i.e.,low gas bearing capacity and low gas pressure in the 'Three Soft' coal seam in western Henan,we applied the theories of plate tectonics and regional structural evolution to investigate the mechanism of this seam and its impact on the coal seam gas formation.Our investigation revealed that coal and gas outbursts are distributed in a strip in a NW direction,with a number of high-penetration mines scattered towards the south side and low-gas mines largely located on the north side.We analyzed the statistics of 38 gas explosions and the rock-coal sturdiness number coefficient f of 167 sampling sites in the region and found the gas outburst mechanism that features a 'low indicator outburst phenomenon'.The mechanism is characterized by structural coal as its core,a low gas bearing capacity,low gas pressure and sturdiness coefficient f mostly less than 0.3.Our research results provide a theoretical foundation for effective control of gas disasters. | LEI DongJi LI Chengwu ZHANG Zimin ZHANG Yugui | 2010 | Mining Science and Technology2010，20，5： | 10 |
| 4 | Achieving strength-ductility synergy in cold spray additively manufactured Al/B4C composites through a hybrid post-deposition treatment显示文摘Cold spray additive manufacturing(CSAM) provides a potential solid state manufacturing route to fabricate variety of aluminum matrix composites(AMCs) with reduced possibility of undesired chemical reactions and residual thermal stresses. This study presents a hybrid(i.e. hot compression + hot rolling)post-deposition treatment to reinvigorate the mechanical properties of cold spray additively manufactured Al/B4 C composites. The as-deposited samples were initially subjected to 30% thickness reduction via hot compression treatment at 500°C followed by a hot rolling treatment with 40% thickness reduction in 2 passes. Electron backscatter diffraction(EBSD) and high resolution transmission electron microscopy(HRTEM) results revealed that after hybrid post-deposition treatment(involving 70%accumulative thickness reduction), the aluminum grains in the matrix were extensively refined due to simultaneous operation of continuous dynamic recrystallization(CDRX) and geometric dynamic recrystallization(GDRX). Furthermore, interfacial defects were remarkably reduced while the nature of Al/Al and Al/B4C interfacial bonding was changed from sheer mechanical interlocking to metallurgical bonding which facilitated efficient transference of applied load to uniformly dispersed bimodal B4C particles. As a result, ultimate tensile strength(UTS) and elongation(EL) of the as-deposited sample were simultaneously improved from 37 to 185 MPa and 0.3% to 6.2%, respectively. | Naeem ul Haq Tariq Lawrence Gyansah Xiang Qiu Chunnijia Hasan Bin Awais Chengwu Zheng Hao Du Jiqiang Wang Tianying Xiong | 2019 | Journal of Materials Science & Technology2019，35，6： | 7 |
| 5 | Pathways related to PMA-differentiated THP1 human monocytic leukemia cells revealed by RNA-Seq显示文摘Previous analyses have reported that the human monocytic cell line THP1 can be differentiated into cells with macrophage-like characteristics by phorbol 12-myristate 13-acetate(PMA). However, little is known about the mechanism responsible for regulating this differentiation process. Here, we performed high-throughput RNA-Seq analysis to investigate the genes differently expressed in THP1 cells treated with and without PMA and examined those that may be responsible for the PMA-induced differentiation of monocytes into macrophages. We found 3,000 genes to be differentially expressed after PMA treatment. Gene ontology analysis revealed that genes related to cellular processes and regulation of biological processes were significantly enriched. KEGG analysis also demonstrated that the differentially expressed genes(DEGs) were significantly enriched in the PI3K/AKT signaling pathway and phagosome pathway. Importantly, we reveal an important role of the PI3K/AKT pathway in PMA-induced THP1 cell differentiation. The identified DEGs and pathways may facilitate further study of the detailed molecular mechanisms of THP1 differentiation. Thus, our results provide numerous potential therapeutic targets for modulation of the differentiation of this disease. | ZENG ChengWu WANG WenTao YU XiBao YANG LiJian CHEN ShaoHua LI YangQiu | 2015 | Science China(Life Sciences)2015，58，12： | 7 |
| 6 | Investigation on Multiple Cylindrical Holes Casing Treatment for Transonic Axial Compressor Stability Enhancement显示文摘Casing treatment is one possible way of regaining axial compressor operating range. However, most of casing treatments extend the operating range with the cost of efficiency penalty. A new form of multiple cylindrical holes casing treatment(MHCT) with pre-swirl blowing for the NASA Rotor-37 has been designed based on profound understanding of the stall inception. Unsteady numerical simulations have been performed for Rotor-37 with and without MHCT. Parametric studies of the total extraction holes area and their axial locations show that the compressor performance deteriorates as the area ratio increases but the stall margin is extended and there is an optimum extraction holes axial location for stall margin extending. The better configuration of MHCT could extend the stall margin by 6.2% with only 0.23% peak efficiency reduction. Detailed analysis of the physical mechanism behind the stall margin improvement shows that the casing treatment could eliminate the passage blockage by suppressing breakup of tip leakage vortex and decrease the blade load in tip region, which both contribute to improve stall margin of transonic axial compressors. | YANG Chengwu ZHAO Shengfeng LU Xingen HAN Ge ZHU Junqiang | 2014 | Journal of Thermal Science2014，23，4： | 6 |
| 7 | Co-Production of Lipids, Eicosapentaenoic Acid, Fucoxanthin, and Chrysolaminarin by Phaeodactylum tricornutum Cultured in a Flat-Plate Photobioreactor Under Varying Nitrogen Conditions显示文摘The marine diatom Phaeodactylum tricornutum is a polymorphological, ecologically significant, and well-studied model of unicellular microalga. This diatom can accumulate diverse important metabolites. Herein, we cultured P. tricornutum in an internally installed tie-piece flat-plate photobioreactor under 14.5 m mol L^(-1)(high nitrogen, HN) and 2.9 m mol L^(-1)(low nitrogen, LN) of KNO_3 and assessed its time-resolved changes in biochemical compositions. The results showed that HN was inductive to accumulate high biomass(4.1 g L^(-1)). However, the LN condition could accelerate lipid accumulation in P. tricornutum. The maximum total lipid(TL) content under LN was up to 42.5% of biomass on day 12. Finally, neutral lipids(NLs) were 63.8% and 75.7% of TLs under HN and LN, respectively. The content of EPA ranged from 2.3% to 1.5% of dry weight during the growth period under the two culture conditions. Peak volumetric lipid productivity of 128.4 mg L^(-1)d^(-1) was achieved in the HN group(on day 9). The highest volumetric productivity values of EPA, chrysolaminarin, and fucoxanthin were obtained in the exponential phase(on day 6) under HN, which were 9.6, 93.6, and 4.7 mg L^(-1)d^(-1), respectively. In conclusion, extractable amounts of lipids, EPA, fucoxanthin, and chrysolaminarin could be obtained from P. tricornutum by regulating the culture conditions. | GAO Baoyan CHEN Ailing ZHANG Wenyuan LI Aifen ZHANG Chengwu | 2017 | Journal of Ocean University of China2017，16，5： | 6 |
| 8 | mrle, a conotoxin from Conus marmoreus with a novel disulfide pattern显示文摘 | Yanfang Wang Xiaoxia Shao Min Li Sumin Wang Chengwu Chi Chunguang Wang | 2008 | Acta Biochimica et Biophysica Sinica2008，40，5： | 5 |
| 9 | Two Potent α3/5 Conotoxins from Piscivorous Conus achatinus显示文摘每个锥蜗牛生产不同 conotoxins 的混合物并且分泌他们使他们的猎物和食肉动物不能调动。α 3 /5 Conotoxins,从狩猎鱼的锥蜗牛孤立,目标肌肉烟碱的醋胆素受体。从食鱼的 Conus achatinus 的α 3 /5 conotoxin 的结构和功能没被学习。我们综合了二 pentadecamer 肽, Ac1.1a 和 Ac1.1b 与适当二硫化物结合,基于α 3 /5 conotoxinsfrom C 的 cDNA 序列。achatinus。Ac1.1a 和 Ac1.1b 由一仅仅氨基酸残余不同。他们分别地在堵住在 Xenopus laevisoocytes 表示的 recombinant 老鼠肌肉醋胆素受体上有类似的力量,与 36 nM 和 26 nM 的 IC_(50 ) 价值。为 Ac1.1b,开始的三 N 终端氨基酸的删除没改变它的活动,显示 N 终点不涉及和它的受体的相互作用。而且,我们的实验显示两毒素强烈在老鼠肌肉上比较喜欢α _1- δ子单元接口而不是α _1- γ结合部位烟碱的醋胆素受体。这些肽为结构的学习和烟碱的受体的功能提供另外的工具。 | Li LIU Geoffrey CHEW Edward HAWROT Chengwu CHI Chunguang WANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，6： | 5 |
| 10 | ANALYTICAL SOLUTIONS TO STRESS CONCENTRATION PROBLEM IN PLATES CONTAINING RECTANGULAR HOLE UNDER BIAXIAL TENSIONS显示文摘The stress concentration problem in structures with a circular or elliptic hole can be investigated by analytical methods. For the problem with a rectangular hole, only approximate results are derived. This paper deduces the analytical solutions to the stress concentration problem in plates with a rectangular hole under biaxial tensions. By using the U-transformation technique and the finite element method, the analytical displacement solutions of the finite element equations are derived in the series form. Therefore, the stress concentration can then be discussed easily and conveniently. For plate problem the bilinear rectangular element with four nodes is taken as an example to demonstrate the applicability of the proposed method. The stress concentration factors for various ratios of height to width of the hole are obtained. | Yi Yang Jike Liu Chengwu Cai | 2008 | Acta Mechanica Solida Sinica2008，21，5： | 4 |
| 11 | Expressed sequence tags analysis revealing the taxonomic position and fatty acid biosynthesis in an oleaginous green microalga,Myrmecia incisa Reisigl(Trebouxiophyceae,Chlorophyta)显示文摘cDNA library of Myrmecia incisa H4301 was constructed using λ phage vectors.The library consisted of 1.5×10 6 clones with a recombination rate of 90%,and 1942 clones were randomly sequenced.All 1854 readable expressed sequence tags(ESTs) were clustered into 596 non-redundant sequences(NRSs),among which 126 NRSs were from 1384 ESTs,showing a high redundancy.Among the 596 NRSs,30 were ribosomal RNA,and 152 significantly matched with those available in NCBI database and JGI Genome Portal,the latter were divided into nine subcategories.Overall,59 NRSs were involved in photosynthesis,the respiratory electron transport chain,ATP synthesis,oxidation reduction,fatty acid biosynthesis,glucose metabolism,protein metabolism,and small molecular metabolism,suggesting that these genes were abundantly transcribed during energy and substance metabolism.Acyl-carrier protein,ferrodoxin and fatty acid elongase genes obtained from this cDNA library enabled presumption of a possible biosynthesis pathway of ArA in M.incisa.Codon usage analysis of 142 NRSs with 17798 codons in the predicted coding regions showed that the average G+C content level of the total codons was 55.39%,and that of the third position in base trimers was 66.42%,indicating a strong bias toward cytosine and/or guanosine in this algal genome.Among all synonymous codons,NAG was most favored,while NUA was most avoided.Phylogenic trees inferred from ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit genes and the extra partial sequences of 18S rRNA obtained from this library demonstrated that M.incisa belonged to Trebouxiophyceae and was significantly clustered with M.incisa SAG 2007,Lobosphaera tirolensis,M.bisecta,and Parietochloris incisa,but was clearly distant from P.pseudoalveolaris and P.alveolar.Transmission electron microscopy revealed pyrenoids traversed by many parallel thylakoids membranes,while starch grains were only clearly observed when cells were grown under nitrogen stress.Based on combined investigation of the phylogeny and morphological characteristics,it is proposed that M.incisa be kept in the genus Myrmecia in which there might be two parallel groups,one living freely and another symbiotic species. | OUYANG LongLing DU DaoHai YU ShuiYan LI ChunYang ZHANG ChengWu GAO HongJian ZHOU ZhiGang | 2012 | Chinese Science Bulletin2012，57，25： | 4 |
| 12 | Research on the degradation mechanism of pyridine in drinking water by dielectric barrier discharge显示文摘Pyridine, an important chemical raw material, is widely used in industry, for example in textiles,leather, printing, dyeing, etc. In this research, a dielectric barrier discharge(DBD) system was developed to remove pyridine, as a representative type of nitrogen heterocyclic compound in drinking water. First, the influence of the active species inhibitors tertiary butanol alcohol(TBA),HCO_3^-, and CO_3^(2-)on the degradation rate of pyridine was investigated to verify the existence of active species produced by the strong ionization discharge in the system. The intermediate and final products generated in the degradation process of pyridine were confirmed and analyzed through a series of analytical techniques, including liquid chromatography–mass spectrometry(LC–MS), high performance liquid chromatography(HPLC), ion chromatography(IC), total organic carbon(TOC) analysis, ultraviolet(UV) spectroscopy, etc. The results showed that the degradation of pyridine was mainly due to the strong oxidizing power of ozone and hydroxyl radical produced by the DBD system. Several intermediate products including 3-hydroxyl pyridine, fumaric acid, 2, 3-dihydroxypyridine, and oxalic acid were detected. Nitrogen was removed from the pyridine molecule to form nitrate. Through analysis of the degradation mechanism of pyridine, the oxidation pathway was deduced. The study provided a theoretical and experimental basis for the application of DBD strong ionization discharge in treatment of nitrogen heterocyclic compounds in drinking water. | Yang Li Rongjie Yi Chengwu Yi Biyun Zhou Huijuan Wang | 2017 | Journal of Environmental Sciences2017，29，3： | 4 |
| 13 | Taxonomy and ultrastructure of five species of Tetraselmis (Prasinophyceae) isolated from China seas显示文摘Comparative ultrastructural investigations on twenty-three isolates of Tetraselmis from the South China Sea, East China Sea and Huanghai Sea, have revealed that these isolates belong to:(1)Tetraselmis chui Butcher; (2)T. cordiformis (H. J. Carter) Stein; (3)T. helgolandica Kylin; (4)T. suecica (Kylin) Butcher; (5)T. guangdongensis sp. nov. Except T. helgolandica, the others are new records in China. T. guangdongensis sp. nov. is a new species. Its external and anatomical features closely resemble those of T. impellucida McLachlan et Parke. As in that species the pyrenoid is penetrated from many directions by cytoplasmic channels delimited by a double membrane. The protoplast withdraws from the apical portion of the theca, that portion which overlaid the trough inverts and pops out in the form of a teat. But unlike that species a starch sheath is present; pyrenoid matrix is surrounded by thylakoids which intervenes between the matrix and the starch sheath surrounding pyrenoid; the theca is stratified. | Zhang Chengwu Hu Hongjun | 2002 | Acta Oceanologica Sinica2002，21，4： | 3 |
| 14 | Mechanical Properties and Temper Resistance of Deformation Induced Ferrite in a Low Carbon Steel显示文摘The microstructures and mechanical properties of deformation induced ferrite(DIF) in the low carbon steel Q235 under different deformation temperatures have been investigated systematically.Through deformation induced ferrite transformation(DIFT),ferrite grain can be refined to 3 μm and accounts for above 85% of the overall fraction.Yield strength of DIF(>500 MPa) is increased by up to 100% compared with the conventional low carbon steel.Comparison of microstructure and mechanical properties in the Q235 steel with DIF and tempered DIF microstructure illustrates that the strengthening mechanism of DIF microstructure is the combination of grain boundary strengthening and carbon supersaturated strengthening.Electron back-scattered diffraction(EBSD) analysis and high magnification scanning electron microscopy(SEM) observation denote that high-angle grain boundary among ultrafine ferrite grain and the transformation product of retain austenite membrane along ferrite boundaries are responsible for the stability of ferrite grain size during tempering process.Transmission electron microscopy(TEM) analysis demonstrates that the transformation product of retained austenite membrane between ferrite grain boundaries is cementite. | Luhan Hao,Namin Xiao,Chengwu Zheng and Dianzhong Li Shenyang National Laboratory for Materials Science,Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China | 2010 | Journal of Materials Science & Technology2010，26，12： | 3 |
| 15 | Geochemistry and Sr-Nd-Pb Isotopes of the Granites from the Hashitu Mo Deposit of Inner Mongolia, China: Constraints on Their Origin and Tectonic Setting显示文摘The Hashitu molybdenum deposit is located in the southern part of the Great Hinggan Range,NE China.Molybdenum mineralization is hosted by and genetically associated with monzogranite and porphyritic syenogranite.Sr-Nd-Pb isotopes of the intrusions show that the porphyritic syenogranite has initial^(87)Sr/^(86)Sr ratios of 0.70418-0.70952,ε_(Nd)(t)values of 1.3 to 2.1(t=143Ma),^(206)Pb/^(204)Pb ratios of 19.191-19.573,^(207)Pb/^(204)Pb ratios of 15.551-15.572,and^(208)Pb/^(204)Pb ratios of38.826-39.143.The monzogranite has initial^(87)Sr/^(86)Sr ratios of 0.70293-0.71305,ε_(Nd)(t)values of 1.1 to2.0(t=147 Ma),^(206)Pb/^(204)Pb ratios of 19.507-20.075,^(207)Pb/^(204)Pb ratios of 15.564-15.596,and^(208)Pb/^(204)Pb ratios of 39.012-39.599.The calculated Nd model ages(T_(DM))for monzogranite and porphyritic syenogranite range from 866 to 1121 Ma and 795 to 1020 Ma,respectively.The granitic rocks in the Hashitu area have the same isotope range as granites in the southern parts of the Great Hinggan Range.The isotope composition indicates that these granites are derived from the partial melting of a juvenile lower crust originating from a depleted mantle with minor contamination by ancient continental crust.The integrating our results with published data and the Late Mesozoic regional tectonic setting of the region suggest that the granites in the Hashitu area formed in an intra-continent extensional setting,and they are related to the thinning of the thickened lithosphere and upwelling of the asthenosphere. | DING Chengwu DAI Pan BAGAS Leon NIE Fengjun JIANG Sihong WEI Junhao DING Chengzhen ZUO Pengfei ZHANG Ke | 2016 | Acta Geologica Sinica(English Edition)2016，90，1： | 3 |
| 16 | Image decomposition using adaptive regularization and div(BMO)显示文摘In order to avoid staircasing effect and preserve small scale texture information for the classical total variation regularization, a new minimization energy functional model for image decomposition is proposed. Firstly, an adaptive regularization based on the local feature of images is introduced to substitute total variational regularization. The oscillatory component containing texture and/or noise is modeled in generalized function space div (BMO). And then, the existence and uniqueness of the minimizer for proposed model are proved. Finally, the gradient descent flow of the Euler-Lagrange equations for the new model is numerically implemented by using a finite difference method. Experiments show that the proposed model is very robust to noise, and the staircasing effect is avoided efficiently, while edges and textures are well remained. | Chengwu Lu Guoxiang Song | 2011 | Journal of Systems Engineering and Electronics2011，22，2： | 2 |
| 17 | Oxidative degradation of phenol by sulfidated zero valent iron under aerobic conditions:The effect of oxalate and tripolyphosphate ligands显示文摘After adding either organic or inorganic ligands,sulfidated nano-zero-valent iron(Sn ZVI)was used for aerobic degradation of phenol,and the effect of the ligand species on oxidation performance was investigated.We found that Sn ZVI hardly degraded phenol in the absence of ligand addition.Ligands initiated and promoted the degradation of pollutants by Sn ZVI.The data herein show that a characteristic inorganic ligand,tripolyphosphate(TPP),is more effective in enhancing oxidation than a characteristic organic ligand oxalate.In addition to the scavenging of reactive oxidants by the organic ligand,more ferrous ion(Fe(Ⅱ))dissolution from Sn ZVI in the TPP system is another cause for the superior enhancement by the inorganic ligand.In the oxalate system,as the sulfur content of Sn ZVI increased,the oxidation efficiency increased because Fe S shell promoted the transfer of electrons to produce more reactive oxygen species(ROS).In TPP system,the effect of sulfur content on oxidation performance is more complex.The Sn ZVI with low sulfur content showed poor oxidation performance compared with that of n ZVI.Further experiments proved that sulfidation might weaken the complexation of TPP with surface bound Fe,which would slow down the ionic Fe(II)dissolution rate.Therefore,sulfidation has the dual effects of enhancing electron transfer and inhibiting the complexation of inorganic ligands.In addition,the mechanisms of ROS generation in different ligand systems were investigated herein.Results showed that the critical ROS in both the oxalate and TPP systems are hydroxyl radicals,and that they are produced via one-electron activation of O_(2). | Xianglong Kong Chengwu Zhang Jingyi Zhang Lishuang Xuan Chuanyu Qin | 2021 | Journal of Environmental Sciences2021，33，2： | 2 |
| 18 | Effects of Temperature, Salinity, Light Intensity, and pH on the Eicosapentaenoic Acid Production of Pinguiococcus pyrenoidosus显示文摘The effects of temperature, light intensity, salinity, and initial pH on the growth and fatty acid composition of Pinguiococcus pyrenoidosus 2078 were studied for eicosapentaenoic acid (EPA) production potential. The fatty acid composition was assayed by gas chromatography-mass spectrometry, which indicated that the main fatty acids were C14:0, C16:0 and EPA. The highest EPA percentage 20.83% of total fatty acids was obtained at 20℃ with the temperature being set at 20, 24, and 28℃. Under different salinities and light intensities, the highest percentages of total polyunsaturated fatty acids (PUFAs) and EPA were 17.82% and 31.37% of total fatty acids, respectively, which were achieved at salinity 30 and 100μmol photon m-2s-1 illumination. The highest percentages of total PUFAs and EPA were 38.75% and 23.13% of total fatty acids, respectively, which were reached at an initial pH of 6 with the test range being from 5.0 to 9.0. | SANG Min WANG Ming LIU Jianhui ZHANG Chengwu LI Aifen | 2012 | Journal of Ocean University of China2012，11，2： | 2 |
| 19 | A Multiplex PCR Assay for the Detection of Pathogenic Genes of EPEC,ETEC and EIEC显示文摘A multiplex polymerase chain reaction (PCR) was developed to detect three pathogenic genes of en-teropathogenic, enterotocigenic and enteroinvasive Escherichia coli.. In this study three different sets of oligonu-cleotide primer were simultaneously used, and in this way, specific fragments of 880, 600, 150 bp for EPEC eaeA, EIEC ipaH and ETEC ST genes were amplified, respectively. The best condition of the multiplex PCR was: after an initial heat denaturation step at 95℃ for 5 min, followed by 30 cycles of denaturation at 94℃ for 40 s, primer annealing at 51.3 ℃ for 40 s and extension at 72 ℃ for 1 min, final extension at 72 ℃ for 10 min. The detection limit of the eaeA, ipaH and ST primers was 38.7423, 3.60519, 29.9448 ng·mL-1 (4.3×104, 1.5×103, 2.6×104 CFU·mL-1), respectively. It may be a good way for the detection and identification of Diarrhea-causing E. coli. . | ZHANG Tienan LI Jichang LU Chengwu HUO Guicheng | 2006 | Journal of Northeast Agricultural University(English Edition)2006，13，1： | 2 |
| 20 | Mitochondria targeting drugs for neurodegenerative diseases——Design, mechanism and application显示文摘Neurodegenerative diseases(NDDs) such as Alzheimer’s disease(AD) and Parkinson ’s disease(PD) are a heterogeneous group of disorders characterized by progressive degeneration of neurons.NDDs threaten the lives of millions of people worldwide and regretfully remain incurable.It is well accepted that dysfunction of mitochondria underlies the pathogenesis of NDDs.Dysfunction of mitochondria results in energy depletion,oxidative stress,calcium overloading,caspases activation,which dominates the neuronal death of NDDs.Therefore,mitochondria are the preferred target for intervention of NDDs.So far various mitochondria-targeting drugs have been developed and delightfully some of them demonstrate promising outcome,though there are still some obstacles such as targeting specificity,delivery capacity hindering the drugs development.In present review,we will elaborately address 1) the strategy to design mitochondria targeting drugs,2) the rescue mechanism of respective mitochondria targeting drugs,3) how to evaluate the therapeutic effect.Hopefully this review will provide comprehensive knowledge for understanding how to develop more effective drugs for the treatment of NDDs. | Jiajia Xu Wei Du Yunhe Zhao Kahleong Lim Li Lu Chengwu Zhang Lin Li | 2022 | Acta Pharmaceutica Sinica B2022，12，6： | 2 |
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