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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Measuring the symptom experi- ence of Chine~ cancer patients:a validation of the Chinese version of the memorial symptom assessment scale显示文摘 | Cheng KK Wong EM Ling WM | 2009 | J Pain Symptom Manage2009，37，1： | 1 |
| 3 | Life long endogenous es- trogen exposure and later adulthood cognitive function in a population of naturally postmenopausal women from Southern China:the Guangzhou Biobank Cohort Study 显示文摘 | Heys M Jiang C Cheng KK | 2011 | Psychoneu- roendocrinology2011，36，6： | 1 |
| 4 | Endocytic carboxylated nanodiamond for the labeling and tracking of cell division and differentiation in cancer and stem cells 显示文摘 | Liu KK Wang CC Cheng CL | 2009 | Biomaterials2009，30，26： | 1 |
| 5 | The role of heat shock protein 70 in the protective effect of YC- 1 on heat stroke rats 显示文摘 | Lam KK Cheng PY Lee YM | 2013 | Eur J Pharmacol2013，15，13： | 1 |
| 6 | Height,its compo- nents,and coagulability among older Chinese :The Guangzhou biobank cohort study 显示文摘 | Zhong Y Jiang CQ Cheng KK | 2014 | Am J Hum Biol2014，26，5： | 1 |
| 7 | Vascular effects of adiponectin: molecular mechanisms and potential therapeutic intervention 显示文摘 | Zhu W Cheng KK Vanhoutte PM | 2008 | Clin Sci (Lond)2008，114，5： | 1 |
| 8 | Cross-talk between adiposetissue and vasculature : role of adiponectin 显示文摘 | Li FY Cheng KK Lam KS | 2011 | Acta Physiol(Oxf)2011，203，1： | 1 |
| 9 | Does smoking status influence the prognosis of bladder cancer? A systematic review显示文摘 | Aveyard P Adab P Cheng KK | 2002 | BJU Int2002，90，： | 1 |
| 10 | Leg length is associat- ed with lower values of inflammatory markers in older Chinese:The Guangzhou Biobank Cohort Study 显示文摘 | Sun Y Jiang CQ Cheng KK | 2015 | Ann Hum Biol2015，42，2： | 1 |
| 11 | APPL1 eouuteraets obesity-induced vaseular insulin resistanee and endothelial dysfunetion by modulating the endothelial produetion of nitrie oxide and endothelin-1 in miee显示文摘 | Wang Y Cheng KK Lam KS | 2011 | Diabetes2011，60，11： | 1 |
| 12 | Adiponectin-induced endothelial nitric oxide synthase activation and nitric oxide production aremediated by APPL1 in endothelial cells显示文摘 | Cheng KK Lam KS Wang Y | 2007 | Diabetes2007，56，： | 1 |
| 13 | Association of plasma methotrexate, neutropenia, hepatic dysfunction, nausea/vomiting and oral mucositis in children with cancer 显示文摘 | Cheng KK | 2008 | Eur J Cancer Care (Engl)2008，17，3： | 1 |
| 14 | APPL1 counteracts obesityinduced vascular insulin resistance and endothelial dysfunction by modulating the endothelial production of nitric oxide and endothelin-1 in mice 显示文摘 | Wang Y Cheng KK Lam KS | 2011 | Diabetes2011，60，11： | 1 |
| 15 | APPL1 counteracts obesity-in-duced vascular insulin resistance and endothelial dysfunction bymodulating the endothelial production of nitric oxide and endothelin-1 in mice显示文摘 | Wang Y Cheng KK Lam KS | 2011 | Diabetes2011，60，11： | 1 |
| 16 | Vascular effects of adiponectin:molecular mechanisms and potential therapeutic intervention显示文摘 | Zhu W Cheng KK Vanhoutte PM | | 0，，： | 1 |
| 17 | Systemic hyperten- sion and glaucoma: mechanisms in common and co-occurrence 显示文摘 | Langman MJ Lancashire RJ Cheng KK | 2005 | Br J Ophthalmol2005，89，8： | 1 |
| 18 | Performance of laboratory di- agnostics for the detection of influenza A (HI NI ) virus as correlated with the time after symptom onset and viral load 显示文摘 | CHENG PK WONG KK MAK GC | 2010 | J Clin Virol2010，47，2： | 1 |
| 19 | Ginsenoside Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats显示文摘 | Cho WC Chung WS Lee SK Leung AW Cheng CH Yue KK | 2006 | Eur J Pharmacol2006，550，13： | 1 |
| 20 | Epidemiology and clinical fea- tures of Shewanella infection over an eight-year period显示文摘 | To KK Wong SS Cheng VC | 2010 | Scand J Infect Dis2010，42，： | 1 |
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