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| 1 | MicroRNA-143 suppresses gastric cancer cell growth and induces apoptosis by targeting COX-2显示文摘AIM:To investigate the function of microRNA-143(miR-143)in gastric cancer and explore the target genes of miR-143.METHODS:A quantitative real-time reverse transcription-polymerase chain reaction(RT-PCR)analysis was performed to evaluate miR-143 expression in gastric cancer cell lines.After transfecting gastric cancer cells with miR-143-5p and miR-143-3p precursors,Alamar blue and apoptosis assays were used to measure the respective proliferation and apoptosis rates.Cyclooxygenase-2(COX-2)expression was determined by realtime RT-PCR and Western blot assays after miR-143transfection.Reporter plasmids were constructed,and a luciferase reporter assay was used to identify the miR-143 binding site on COX-2.RESULTS:Both miR-143-5p and miR-143-3p were significantly downregulated in multiple gastric cancer cell lines.Forced miR-143-5p and miR-143-3p expression in gastric cancer cells produced a profound cytotoxic effect.MiR-145-5p transfection into gastric cancer cells resulted in a greater growth inhibitory effect(61.23%±3.16%vs 46.58%±4.28%,P<0.05 in the MKN-1cell line)and a higher apoptosis rate(28.74%±1.93%vs 22.13%±3.31%,P<0.05 in the MKN-1 cell line)than miR-143-3p transfection.Further analysis indicated that COX-2 expression was potently suppressed by miR-143-5p but not by miR-143-3p.The activity of a luciferase reporter construct that contained the 3’-untranslated region(UTR)of COX-2 was downregulated by miR-143-5p(43.6%±4.86%,P<0.01)but not by miR-143-3p.A mutation in the miR-145-5p binding site completely ablated the regulatory effect on luciferase activity,which suggests that there is a direct miR-145-5p binding site in the 3’-UTR of COX-2.CONCLUSION:Both miR-143-5p and miR-143-3p function as anti-oncomirs in gastric cancer.However,miR-143-5p alone directly targets COX-2,and it exhibits a stronger tumor suppressive effect than miR-143-3p. | Xiao-Li Wu Bin Cheng Pei-Yuan Li Huan-Jun Huang Qiu Zhao Zi-Li Dan Dean Tian Peng Zhang | 2013 | World Journal of Gastroenterology2013，19，43： | 15 |
| 2 | MicroRNA Expression Signature and Antisense-Mediated Depletion Reveal an Essential Role of MicroRNA in Vascular Neointimal Lesion Formation显示文摘 | Ruirui Ji Yunhui Cheng Junming Yue Jian Yang Xiaojun Liu He Chen David B. Dean Chunxiang Zhang | 2007 | Circulation Research2007，，11： | 4 |
| 3 | Regioselective oxidation of tetracycline by permanganate through alternating susceptible moiety and increasing electron donating ability显示文摘Permanganate has attracted much attention in wide range of chemistry and particularly in degradation of environmental pollutants.However,few studies have discussed the feature of regioselective reactivity of permanganate with specific moiety of the target compound.Herein,we studied the reaction between permanganate and tetracycline that is an emerging micropollutant with different species containing several electron-rich groups.The second-order rate constants increased from 6.0 to 9.0 and could be quantitatively modeled by considering the speciation of both reactants,yielding kTC0=11.7(mol/L)^-1 sec^-1,kTC-=35.7(mol/L)^-1 sec^-1,kTC2-=43.1(mol/L)^-1 sec^-1 for individual reaction channels.Degradation products were then identified as the hydroxylated and demethylated compounds.The result suggested a rate-limiting step of simple hydroxylation at the phenolic and/or alkene moieties,while the demethylation should be caused by the unavoidably formed manganese oxide via single electron oxidation.This is supported by the DFT calculation,indicating the primary oxidation of phenolic group of TC0 with activation barrier of 44.5 kcal/mol and of alkene group of TC-and TC2-with activation barriers of 44.0 and 43.4 kcal/mol,respectively.This is in agreement with the experimental results,implying the alternation of regioselectivity associated with the deprotonation process.The result was further supported by performing the Fukui function and electrostatic potential analysis,reflecting the more probable site and better electron donating tendency beneficial to the permanganate oxidation. | Xiaohua Jiang William AJefferson Dean Song Hanyang Cheng Fengmin Li Zhimin Qiang Aiqian Zhang Huijuan Liu Jiuhui Qu | 2020 | Journal of Environmental Sciences2020，32，1： | 1 |
| 4 | Hydrodynamic evaluation of hull forms with podded propulsors显示文摘 | Cheng B H Dean J S Cave W L | 1989 | Naval Engineers Journal1989，101，： | 1 |
| 5 | Serine/threonine protein phosphatase type 1γ1 is required for the completion of cytokinesis in human A549 lung carcinoma cells 显示文摘 | Cheng A Dean N M Honkanen R E | 2000 | J Biol Chem2000，275，3： | 1 |
| 6 | Low cost synthesis of LiFePO 4 /C cathode materials with Fe 2 O 3显示文摘 | Lifeng Cheng Guoxian Liang Soumia El Khakani Dean D. MacNeil | 2013 | Journal of Power Sources2013，，： | 1 |
| 7 | Phase characterization and tunable photoluminescence of Eu-doped strontium-substituted nanohalophosphate 显示文摘 | Yang Chih Cheng Chen San Yuan Liu Dean Mo | 2006 | Journal of Crystal Growth2006，293，： | 1 |
| 8 | PolySearch: a web- based text mining system extracting relationships between hu- man diseases,genes,mutations,drugs and metabolites显示文摘 | Cheng Dean Craig Knox Nelson Young | 2008 | Nucleic Acids Research2008，36，2： | 1 |
| 9 | Automatic recognition vision system guided for apple harvesting robot显示文摘 | Ji Wei Zhao Dean Cheng Fengyi | 2012 | Computers and Electrical Engineering2012，38，5： | 1 |
| 10 | Micropropagation of Populus trichocarpa ‘Nisqually-1’: the genotype deriving the Populus reference genome显示文摘 | Byung-guk Kang Lori Osburn Dean Kopsell Gerald A. Tuskan Zong-Ming Cheng | 2009 | Plant Cell Tissue and Organ Culture (PCTOC)2009，，3： | 1 |
| 11 | MicroRNAs Are Aberrantly Expressed in Hypertrophic Heart显示文摘 | Yunhui Cheng Ruirui Ji Junming Yue Jian Yang Xiaojun Liu He Chen David B. Dean Chunxiang Zhang | 2007 | The American Journal of Pathology2007，，6： | 1 |
| 12 | Automatic recogni- tion vision system guided for apple harvesting robot 显示文摘 | Wei Ji Dean Zhao Fengyi Cheng | 2012 | Computers & Electrical Engineering2012，38，5： | 1 |
| 13 | Pressure transients and crossflow caused by diffusivities in multilayer reservoirs 显示文摘 | Gao Cheng Tai Deans H A | 1988 | SPEFE1988，，6： | 1 |
| 14 | Evaluation of hydro-mechano-chemical behaviour of bentonite-sand mixtures显示文摘Bentonite-sand mixtures are widely used in engineering barrier of deep geological disposal of high-level radioactive nuclear waste and anti-seepage barrier of civil geotechnical engineering.Under the action of groundwater solution infiltration and external stress,the hydro-mechanical(HM)behaviour of bentonitesand mixtures,i.e.the swelling characteristics and permeability,will change.Once the anti-seepage and filtration effect is weakened or lost,the pollutants will spread to the biosphere.Therefore,it is necessary to study the swelling characteristics and permeability of bentonite-sand mixtures under coupled mechanochemical(MC)effect and to establish corresponding prediction model.For this reason,swelling tests under salt solution with different concentrations are conducted on pure bentonite and its mixtures with 30%,70%and 90%sand contents,the compression tests are carried out on saturated samples,and the saturated permeability coefficient k of the sample under each load is calculated by Terzaghi’s one-dimensional consolidation theory.The concepts of true effective stress pe,montmorillonite void ratio em and critical sand content as are introduced to determine the em-pe relationship and finally the k-em relationship of bentonite-sand mixtures.It is found that when the sand content aas,the em-pe relationship of the mixture is linear and independent of the salt solution concentration,and when a>as,the em-pe relationship of bentonite-sand mixture is bi-linear with the true effective deviatoric stress pesa as the intersection.In addition,the em-k relationship also shows the linear trend when aas,and the slope of the line increases with the increase of the salt solution concentration.When a>as,the k-em relationship will deviate from the linear relationship.Moreover,the larger the sand content is,the farther the deviation is.On the basis of summing the regularity,a model for predicting the HM behaviour of bentonite-sand mixture under the coupled MC effect is proposed.By comparing the swelling and permeability test results with model prediction results of different types of bentonite and its sand mixtures,the predictive model is verified.The study on the HM behaviour of bentonite-sand mixtures under salt solution infiltration and the model establishment can provide experimental and theoretical basis for the design and construction of anti-seepage engineering by bentonite-sand mixtures. | Wenjing Sun Cheng Liu Diansen Yang Dean Sun | 2022 | Journal of Rock Mechanics and Geotechnical Engineering2022，14，2： | 1 |
| 15 | Automatic recognition vision system guided for apple harvesting robot显示文摘 | Wei Ji Dean Zhao Fengyi Cheng Bo Xu Ying Zhang Jinjing Wang | 2011 | Computers and Electrical Engineering2011，，： | 1 |
| 16 | MicroRNA Expression Signature and Antisense-Mediated Depletion Reveal an Essential Role of MicroRNA in Vascular Neointimal Lesion Formation显示文摘 | Ruirui Ji Yunhui Cheng Junming Yue Jian Yang Xiaojun Liu He Chen David B. Dean Chunxiang Zhang | 2007 | Circulation Research2007，，11： | 1 |
| 17 | Serine/threonine protein phosphatase type 11 is required for the comple- tion of cytokinesis in human A549 lung carcinoma cells 显示文摘 | Cheng A Dean N M Honkanen R E | 2000 | J Biol Chem2000，275，： | 1 |
| 18 | 亭仔脚——生活记忆的延续显示文摘在台湾早期农村社会有一种人文特色,俗称“亭仔脚”。这是农村生活里人际关系的交流方式,在快速变化的社会环境中流逝,但却深植于人们的记忆里。本案位于台北市大屯山下北投温泉区,为一对老夫妻所设计,用来作为退休后的居所。于日治时期,这里便是著名的温泉泡汤休憩区。丰富的天然资源,辅以人文气息,奠定了作品的设计主轴为“为空间使用者寻找一个生活的回忆”。设计师透过材料的选择,如回收旧木、水泥、磨石子、榻榻米等,探讨如何透过材料之于空间的表情,呈现在地文化的传递。 | 方信原 Dean Cheng | 2018 | 现代装饰2018，0，10： | 0 |
| 19 | Control Effect of UAV Low Volume Spray Technology on Thrips and Tomato Spotted Wilt Virus in Tobacco Field显示文摘[Objectives]The paper was to explore the influence of unmanned aerial vehicle(UAV)low volume spray technology on the control effect of viruliferous thrips and tomato spotted wilt virus(TSWV)under different pesticide concentrations,and to explore the effective control nodes and methods of thrips and TSWV.[Methods]According to the occurrence characteristics of thrips and TSWV,the field control effects of 4 pesticides on thrips and TSWV were tested by UAV with the thrips number and disease index as indicators,following the principle of regional and periodic control trials.[Results]In the groups of UAV and artificial prevention and control,4 agents were used alternatively,including 1%emamectin benzoate ME,1.7%abamectin imidacloprid ME,5%emamectin benzoate WDG,and 30%pyriproxyfen dinotefuran SL.There was no significant difference in the control effect of thrips and TSWV among treatment 1(recommended dosage of UAV agent),treatment 2(reduction of recommended dosage of UAV agent by 25%)and treatment 4(recommended dosage of artificial control agent),and the control effects on thrips were more than 83.16%in the 3 treatments.The disease index of TSWV in the 3 treatments decreased from 8.64±1.37 in the blank control group to less than 3,which reached the prevention and control threshold.But treatment 3(reduction of recommended dosage of UAV agent by 50%)did not reach the prevention and control threshold.The disease index of TSWV in the blank control area increased with the increase of the number of live nymphs of thrips,and there was a significant positive correlation,with good fitness.[Conclusions]UAV has a significant impact on the control effect of thrips and TSWV under different pesticide concentrations.In the actual flight control application,it is recommended that the amount of the pesticides is about 3/4 and not less than 1/2 of that of the artificial application.The control area should be extended to 100 m of the ridge of the tobacco field,and UAV is used periodically to control thrips and TSWV for 5 times from pre-transplanting stage to pre-squaring stage of flue-cured tobacco.The control effect is significantly different from the traditional artificial application. | Zefan LIU Dean XIE Jiabao HAN Yanfen XIE Li MA Siyuan GAO Ruiqi PENG Yuting ZHAO Xiangze FU Wengang CHENG Yonghong FENG Yan ZHANG Huaping ZHANG Ying HUANG Leijie DAI Lanfeng WANG | 2023 | Plant Diseases and Pests2023，14，4： | 0 |
| 20 | 台北屋顶 中国台北显示文摘台北屋顶位于台北市购物区,是一座12层的集体住宅。台湾人设计出各式各样的居间区,以适应湿热的气候。你可以巧妙地利用贯穿整个城市的道路扩建面的简单环境,看到人们享受吃饭聊天。台湾人既拥有丰富的生活方式,又充满21世纪的新鲜感,有没有可能考虑建造适合他们的公寓楼?因此,我们并没有选择参考基于平面图上的塔式建筑,而是选择了倾斜的形状,同时在对角线上控制范围,这样所有的住宅单元都可以有一个大露台。露台上覆盖着屋顶和树木,在建筑表面创造了一个舒适的三维居间区。 | 无 PAUIYAN archiland(摄影) Dean Cheng(摄影) | 2021 | 世界建筑导报2021，36，3： | 0 |
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