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| 1 | The roles of surfactant protein D during Aspergillus fumigatus infection in human corneal epithelial cells显示文摘AIM: To investigate roles of surfactant protein D (SP-D) and relative cytokines in human corneal epithelial(HCE) cells exposed to aspergillus fumigatus (AF) antigens.METHODS: HCE cells cultured in vitro with AF antigens and sampled at 0, 0.5, 1 hour, 2, 4, 6 and 8 hours. The expression of SP-D mRNA was evaluated by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR).The expression of SP-D protein was shown by ELISA and immunocytochemistry SP methods. The expression of NF-κB and relative downstream cytokines such as TNF-α, IL-1β, IL-8 and IL-10 in supernatant fluid were measured by ELISA.RESULTS: SP-D mRNA and protein were detected in untreated HCE cells. The expression of SP-D and the relative downstream cytokines rose after being stimulated with AF antigens. SP-D mRNA began to rise at 0.5 hour and the most significantly peak was in 2 hours. The protein of SP-D in supernatant fluid had the same trend with mRNA. Immunocytochemistry of SP-D showed positive expression and gradually increased to 6 hours, and then the expression began to decline. NF-κB was activated after treated by AF antigens and the changes had correlation with SP-D. TNF-α, IL-1β, IL-8 and IL-10 began to rise after given AF antigens 1 hour and were 1.82, 1.43, 1.12 and 1.28 times higher than the untreated HCE cells separately. The expression of TNF-α and IL-1β reached the peak at 2 hours, separately 2.80 and 2.86 times than the untreated. The expression of IL-8 and IL-10 gradually increased with a time-dependent manner.CONCLUSION: HCE cells exists SP-D and it may play a significant role in pathogenesis of keratomycosis. AF may induce human corneal epithelial cells to express inflammatory cytokines via SP-D and NF-κB pathway. SP-D possibly mediates the recognition to AF mycelium. | Cheng-Ye Che Wen-Yan Jia Qiang Xu Na Li Li-Ting Hu Nan Jiang Jing Lin Qing Wang and Gui-Qiu Zhao | 2012 | International Journal of Ophthalmology(English edition)2012，5，1： | 12 |
| 2 | Effects of COX-2 inhibitor NS-398 on IL-10 expression in rat fungal keratitis显示文摘·AIM:To investigate the expression of interleukin-10 (IL-10) and the effect of NS-398(COX-2 inhibitor) on the expression of IL-10 in fungal keratitis in rats,and analyze its effects on anti-fungus immunity.·METHODS:Ninety Wister rats were randomly divided into 3 groups.Group A was blank control group (10 eyes).Group B was fungal keratitis group (40 eyes).Group C was fungal keratitis group treated with NS-398 (40 eyes).PAS staining,100g/L potassium hydroxide (KOH) smear and fungal culture confirmed the successful establishment of fungal keratitis model.After the central epithelium was scraped,Fusarium solani colonies were applied and contact lens was put on the right cornea of group B and C,and plane contact lens was put on the left cornea of control eyes.Phosphate buffered saline (PBS) eyedrops were given for group B and NS-398 eyedrops for group C.The expression of IL-10 on corneas of group B and C on the 1st day,3rd days,7th days,and 14th days were detected by immunohistochemistry and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).·RESULTS:Histopathologic examination showed neutrophil infiltration and severe tissue necrosis in ulcer cornea.PAS staining confirmed the existence of hyphae and spores in the superficial layer of stroma.In the blank and control groups almost no expression of IL-10 was detected at any observing points.In group B the expression of IL-10 increased at first and decreased thereafter.Its expression also showed significant difference at any observing points (P￡?0.01).Compared with group B,the expression of IL-10 in group C showed no difference on the 1stday,decrease on the 3rd day,but a significant increase on the 7th day and 14th day.·CONCLUSION:IL-10 takes part in the occurrence and development of fungal keratitis.NS-398 can upgrade the expression of IL-10 in fungal keratitis in the later period of the ulcer.Meanwhile,pathologic observation showed a slightly corneal opacity.IL-10 may play an important role in the process of cornea anti-damage repair.· | Na Li Cheng-Ye Che Li-Ting Hu Jing Lin Qing Wang and Gui-Qiu Zhao | 2011 | International Journal of Ophthalmology(English edition)2011，4，2： | 7 |
| 3 | Expression of dectin-1 during fungus infection in human corneal epithelial cells显示文摘AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1(dectin-1) in human corneal epithelial(HCE) cells infected by fungus. · METHODS: A total of 20 cases of healthy donor corneas were group A,and 20 patients(20 eyes) suffered from fungal keratitis(FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus(AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0,4,8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately. ·RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h,and dectin-1 protein expression increased after stimulation at 24h. · CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro. | Cui Li Gui-Qiu Zhao Cheng-Ye Che Na Li Jing Lin Qiang Xu Qian Wang Ying Liu Sheng Qiu | 2014 | International Journal of Ophthalmology(English edition)2014，7，1： | 6 |
| 4 | Effect of corneal graft diameter on therapeutic penetrating keratoplasty for fungal keratitis显示文摘AIM: To evaluate the effect of corneal graft diameter on therapeutic penetrating keratoplasty(PKP) for fungal keratitis. METHODS: A total of 116 patients (116 eyes) suffered from fungal keratitis underwent PKP at the Affiliated Hospital of Medical College Qingdao University from May 2006 to May 2010. They were divided into two groups according to the corneal graft diameter. 64 eyes' corneal graft diameter was 8.00mm or larger and 52 eyes' graft diameter was smaller than 8.00mm. The follow-up time was 2 years. The postoperative visual acuity and complications were documented and compared. RESULTS: Sixty-two (96.88%) eyes and fifty (96.15%) eyes preserved eyeballs respectively in two groups. There was no statistical difference in postoperative visual acuity (P =0.961), corneal graft clear rate (P =0.132) or the incidence of recurred fungal infection (P =0.770) between two groups. But there was a higher incidence of graft rejection (P =0.020) and secondary glaucoma (P =0.039) in group with corneal graft diameter 8.00mm or larger. CONCLUSION: PKP is an effective treatment approach for fungal keratitis. There is a higher incidence of complications in large-diameter PKP for fungal keratitis.Effective, preventive and therapeutic measures can improve the prognosis. | Cui Li Gui-Qiu Zhao Cheng-Ye Che Jing Lin Na Li Wen-Yan Jia Qiu-QiuZhang Nan Jiang Li-Ting Hu | 2012 | International Journal of Ophthalmology(English edition)2012，5，6： | 5 |
| 5 | Early expression of surfactant proteins D in Fusarium solani infected rat cornea显示文摘AIM:To investigate the early expression of surfactant proteins D(SP-D) in Fusarium solani infected rat cornea.· METHODS:Wistar rats were divided into group A,B and C randomly.The right eyes were chosen as the experiment one.Group A was control group.Group B was not inoculated with Fusarium solani.Group C was taken as fusarium solani keratitis model.Five rats in group B and C were executed randomly at 6,12,24,48 and 96 hours respectively after the experimental model being established.The expression of SP-D was assessed through immunohistochemistry and reverse transcription polymerase chain reaction(RT-PCR).· RESULTS:RT-PCR detected that the SP-D mRNA expression was low in the corneal of normal rats and group B.The expression of fungal infected cornea increased gradually and reached the peak at 24 hours in group C.The synchronous expression of group B and C were in significant difference(P <0.01).Immunohistochemisty discovered the protein of SP-D expression was increased gradually from 12 hours and reached the peak at 48 hours in group C.The synchronous expression of group B and C were also in significant difference(P <0.01).· CONCLUSION:There exists SP-D in rat corneal tissue and the expression is significantly increased at the early period of fusarium solani infected cornea.SP-D may play a role in the early innate immunity response of the corneal resistance to Fusarium solani infection. | Cheng-Ye Che, Gui-Qiu Zhao | 2012 | International Journal of Ophthalmology(English edition)2012，5，3： | 4 |
| 6 | Diagnostic Value of Cerebrospinal Fluid T-SPOT.TB for Tuberculousis Meningitis in China显示文摘The aim of this study was to evaluate the diagnostic value of the cerebrospinal fluid(CSF) T‐SPOT.TB test for the diagnosis of TB meningitis(TBM). A retrospective analysis of 96 patients with manifested meningitis was conducted; T‐SPOT.TB test was performed for diagnosing TBM to determine the diagnostic sensitivity, specificity, positive predictive value(PPV), and negative predictive value(NPV). A receiver operating characteristic(ROC) curve was also drawn to assess the diagnostic accuracy. The sensitivity, specificity, PPV, and NPV of CSF T‐SPOT.TB test were 97.8%, 78.0%, 80.3%, and 97.5%, respectively, for 52 patients(54.2%) of the 96 enrolled patients. The area under the curve(AUC) was 0.910, and the sensitivities of CSF T‐SPOT.TB for patients with stages I, II, and III of TBM were 96.7%, 97.2%, and 98.9%, respectively. CSF T‐SPOT.TB test is a rapid and accurate diagnostic method with higher sensitivity and specificity for diagnosing TBM. | LI Xue Lian XIE Na WANG Song Wang WU Qian Hong MA Yan SHU Wei CHEN Hong Mei ZHANG Li Qun WU Xiao Guang MA Li Ping CHE Nan Ying GAO Meng Qiu | 2017 | Biomedical and Environmental Sciences2017，30，9： | 3 |
| 7 | DEM investigation of rock/bolt mechanical behaviour in pull-out tests显示文摘Rock bolt anchorage performance is crucial for tunnel support safety.We investigate the mechanical behaviour of reinforced rock and the bolts that reinforce it from the micro-scale to the macro-scale.Bolt pull-out tests were performed on soft rock using the distinct element method,in which a new contact model that considers bond size,is employed to constrain the main rock mechanical behaviour.The minimum sample width and height values for which the boundary effect can be neglected are first proposed through numerous tests on the influence of sample size on peak load and bond breakage.The influence of sample width is substantially greater than that of sample height.We then select an appropriate sample size to study the influence of bolt embedment length and confining pressure on the mechanical behaviours of the rock and bolt.The results show that increased rock bolt embedment length and confining pressure can increase the peak load;however,the bolt length effect is limited when exceeding the critical anchorage length.In cases without confining pressure,bond breakage occurs in the rock around the grout-rock interface and the breakage zone is rectangular,whereas in cases under confining pressure,the breakage zone presents an inverted cone shape.We use our results to discuss the influence of bond strength at the bolt-grout interface on the peak load and failure mode.The failure mode changes gradually from complex failure to single failure along the bolt-grout interface with decreasing interfacial bond strength. | Na Che Huaning Wang Mingjing Jiang | 2020 | Particuology2020，18，5： | 2 |
| 8 | A cellular response protein induced during HSV-1 infection inhibits viral replication by interacting with ATF5显示文摘Studies of herpes simplex virus type 1(HSV-1)infection have shown that many known and unknown cellular molecules involved in viral proliferation are up-regulated following HSV-1 infection.In this study,using two-dimensional polyacrylamide gel electrophoresis,we found that the expression of the HSV-1 infection response repressive protein(HIRRP,GI 16552881)was up-regulated in human L02 cells infected with HSV-1.HIRRP,an unknown protein,was initially localized in the cytoplasm and then translocated into the nucleus of HSV-1-infected cells.Further analysis showed that HIRRP represses HSV-1proliferation by inhibiting transcription of the viral genome by interacting with the cellular transcription factor,ATF5,via its N-terminal domain.ATF5 represses the transcription of many host genes but can also act as an activator of genes containing a specific motif.We found that ATF5 promotes the proliferation of HSV-1 via a potential mechanism by which ATF5 enhances the transcription of viral genes during the course of an HSV-1 infection;HIRRP then induces feedback repression of this transcription by interacting with ATF5. | WU LianQiu ZHANG XueMei CHE YanChun ZHANG Ying TANG SongQing LIAO Yun NA RuiXiong XIONG XiangLin LIU LongDing LI QiHan | 2013 | Science China(Life Sciences)2013，56，12： | 2 |
| 9 | Extraction, partial characterization and bioactivity of polysaccharides from boat-fruited sterculia seeds显示文摘 | Lianzhong Ai Jinhong Wu Na Che Yan Wu Steve W. Cui | 2012 | International Journal of Biological Macromolecules2012，，5： | 1 |
| 10 | The effect of enterovirus 71 immunization on neuropathogenesis and protein expression profiles in the thalamus of infected rhesus neonates 显示文摘 | Chen H Zhang Y Yang E Liu L Che Y Wang J Zhao H Tang D Dong C Yang L Shen D Wang X Liao Y Wang L Na R Liang Y Li Q | 2012 | Virology2012，432，2： | 1 |
| 11 | Neonatal rhesus monkey is a potential animal model for studying pathogenesis of EV71 infection显示文摘 | Liu L ZH Zhang Y Wang J Che Y Dong C Zhang X Na R Shi H Jiang L Wang L Xie Z Cui P Li Q | 2011 | Virology2011，412，1： | 1 |
| 12 | Inflammatory microcry stals differenbiallly regnlabe the secrebion of maerophage inflammatory protein 1 and interlenkin 8 by human hewbrophils:a possible mechanism of neutrophil recruitment to sites of inflammation in synovibis显示文摘 | Na cca che PH McColl SR | 1995 | J Exp Med1995，182，： | 1 |
| 13 | Food proteins from different allergen families sensitize Balb/c mice to family-specific immune responses显示文摘 | Jing Wang Na Sun Cui Zhou Xin Zhou Jing Lu Cuiyan Wang Huilian Che | 2013 | Journal of Immunotoxicology2013，，2： | 1 |
| 14 | On improved robust stabilization of uncertain systems with unknown input delay显示文摘 | Wu Hua Che Na Zheng Wei-xing | 2006 | Automatica2006，42，： | 1 |
| 15 | Role of vasoactive intestinal peptide in Aspergillus fumigatus-infected cornea显示文摘AIM: To investigate the anti-inflammatory role of vasoactive intestinal peptide(VIP) in Aspergillus fumigatus(A. fumigatus) ketatitis.METHODS: Expression of VIP was tested by polymerase chain reaction(PCR) in C57BL/6 and BALB/c normal and A. fumigatus infected corneas. C57BL/6 mice were pretreated with recombinant(r) VIP, while BALB/c mice were pretreated with VIP antagonist, and then infected with A. fumigatus. Clinical score was recorded. Expression of pro-and anti-inflammatory cytokines, toll-like receptor 4(TLR4), lectin-like oxidized low-density lipoprotein receptor 1(LOX-1), and neutrophil infiltration were tested by PCR, enzyme-linked immunosorbent assay(ELISA), and myeloperoxidase(MPO) assay.RESULTS: VIP mR NA expression in BALB/c cornea was higher than C57BL/6 cornea at 1 and 3 d post infection(p.i.). rV IP treatment of C57BL/6 mice showed alleviated disease and down-regulated expression of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α), while IL-10 expression was up-regulated. Neutrophil infiltration and TLR4, IL-17 expression were decreased after rVIP treatment, while LOX-1 expression was up-regulated in C57BL/6. VIP antagonist pretreatment showed increased disease and higher IL-1β, TNF-α, TLR4, IL-17 and MPO levels, while IL-10 and LOX-1 levels were down-regulated in BALB/c mice.CONCLUSION: rVIP alleviate disease response of C57BL/6 mice. VIP antagonist resulted in worsened disease of BALB/c mice. VIP proposed anti-inflammatory role in A. fumigatus keratitis. | Cui Li Yuan-Yuan Liu Gui-Qiu Zhao Jing Lin Cheng-Ye Che Nan Jiang Na Li Jie Zhang Kun He Xu-Dong Peng | 2018 | International Journal of Ophthalmology(English edition)2018，11，2： | 1 |
| 16 | ISSR Analysis of Hybrid Descendants of Roses显示文摘Four different rose hybrid populations were analyzed by ISSR technique with nine polymorphic primers. All of the primers produced 64 bands, of which 59 were polymorphic. The average of polymorphic bands was 6.6 for each primer. The percentage of polymorphism ranged from 57.1% to 100%, with an average of 92.3%. An UPGMA dendrogram showed five groups. The primer numbered with PI9 and PI11 had relatively high resolution in identifying the F 1 offspring in this research. | Che Dai-di Su Cheng-yuan Zhang Jin-zhu Chen Xue Ma Xue Wang Na Li Wen-tian | 2013 | Journal of Northeast Agricultural University(English Edition)2013，20，1： | 1 |
| 17 | Coexpression of Bcl-2 with epithelial–mesenchymal transition regulators is a prognostic indicator in hepatocellular carcinoma显示文摘 | Nan Zhao Bao-cun Sun Xiu-lan Zhao Zhi-yong Liu Tao Sun Zhi-qiang Qiu Qiang Gu Na Che Xue-yi Dong | 2012 | Medical Oncology2012，，4： | 1 |
| 18 | Anthocyanin changes in the Korean purple-fleshed sweet potato, Shinzami, as affected by steaming and baking显示文摘 | Heon Woong Kim Jung Bong Kim Soo Muk Cho Mi Nam Chung Young Min Lee Sang Mi Chu Jeong Hwan Che Se Na Kim So Young Kim Young Sook Cho Jae Hyun Kim Hong Ju Park Dong Jin Lee | 2011 | Food Chemistry2011，，： | 1 |
| 19 | An inactivated enterovirus 71 vaccine in healthy children 显示文摘 | Li RC Liu LD Mo ZJ Wang XA Liang ZL Zhang Y Li YP Mao QY Wang JJ Jiang L Dong CH Che YC Huang T Jiang ZW Xie ZP Wang LC Liao Y Liang Y Nong Y Liu JA Na RX Guo L Pu J Yang EX Sun L Cui PF Shi HJ Wang JZ Li QH | 2014 | N Engl J Med2014，370，9： | 1 |
| 20 | Three dimensional BC/rGA aerogel:preparation,characterization,and adsorption of Cr(Ⅵ)显示文摘Graphene-based composite aerogel doped with other low-cost materials can reduce the cost and promote the use in water treatment.This work prepared ball-milled biochar/reduced graphene oxide aerogel(BC/rGA)using GO and low-cost ball-milled biochar(BC)in a certain proportion with the freeze-thawing technique and sol-gel method,and applied BC/rGA on the Cr(Ⅵ)removal from aquatic environments.The characterization results showed that aerogel had a honeycomb briquette three-dimension(3D)and mesoporous structure with interconnected pores,and proved the preparation progress of aerogel in principle.Compared with GO,rGA and BC/rGA had better adsorption performance with 3D structure and well-developed pores,and BC/rGA with the mixture ratio of BC and GO of 1:4 was more appropriate.The adsorption kinetics data of rGA and BC/rGA_((1:4))were fitting well with the pseudo-second-order model(R^(2)>0.951),and the isotherm adsorption results were fitting the Langmuir model well(R^(2)>0.974).The results demonstrated that the adsorption process was monolayer and endothermic adsorption involving chemisorp-tion.Additionally,the adsorption capacities of rGA and BC/rGA_((1:4))at solution pH 2 were 3.71 and 3.89 times greater than those at solution pH 8,respectively.High background ion strength and low temperature slightly inhibited the adsorption of Cr(Ⅵ)by both rGA and BC/rGA_((1:4)).The adsorption mechanisms of Cr(Ⅵ)on rGA and BC/rGA_((1:4))were electrostatic interaction,reduction and ion exchange.The use of BC/rGA could reduce the cost and promote the green reuse of agricultural waste.Overall,BC/rGA could be used as a promising green adsorbent alternative for the feasible treatment of heavy metal contaminated water. | Naiju Che Na Liu Yinhui Li Chao Li Yanli Liu Chengliang Li | 2022 | Biochar2022，4，1： | 0 |
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