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| 1 | Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity显示文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts. | Maria B.Asparuhova Jordi Caballé-Serrano Daniel Buser Vivianne Chappuis | 2018 | International Journal of Oral Science2018，10，4： | 17 |
| 2 | Parental consent in paediatric clinical research显示文摘 | Chappuy H Doz F Blanche S | 2006 | Arch Dis Child2006，91，2： | 1 |
| 3 | A significant response to neoadjuvant chemotherapy in BRCA1/2 related breast cancer显示文摘 | Chappuis PO Goffin J Wong N | | 0，，： | 1 |
| 4 | Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer显示文摘 | Foulkes WD Stefansson IM Chappuis PO | 2003 | J Natl Cancer Res2003，95，19： | 1 |
| 5 | Is the appendix graft suitable for routine bil ary surgery in children ? 显示文摘 | Delarue A Chappuis JP Esposito C | 2000 | J Pediatri Surg2000，35，9： | 1 |
| 6 | Adenocarcinoma of the stomach:a review of 35 years and 1 710 cases显示文摘 | Breaux J R Bringaze W Chappuis C | 1990 | World J Surg1990，14，5： | 1 |
| 7 | Biomechanical evaluation of a novel fenestrated pediele screw augmented with bone cement in osteoporotic spines 显示文摘 | Pare P E Chappuis J L Rampersaud R | 2011 | Spine ( Phila Pa 1976 )2011，36，18： | 1 |
| 8 | Intestinal paracellular permeability during malnutrition in guinea pigs:effect of high dietary zinc 显示文摘 | Darmon N Chappuis P | 1996 | Gut1996，39，： | 1 |
| 9 | Replantation of 45 avulsed permanent teeth: a l-year follow-up study 显示文摘 | Chappuis V von Arx T | 2005 | Dent Traumatol2005，21，5： | 1 |
| 10 | Intestinal paracellular permeability during malnutrition in guinea pigs: effect of high dietary zinc 显示文摘 | Rodriguez P Darmon N Chappuis P | 1996 | Gut1996，39，： | 1 |
| 11 | Twenty-three novel BRCA1 and BRCA2 sequence variations identified in a cohort of Swiss breast and ovarian cancer families显示文摘 | Philippe Maillet Pierre Olivier Chappuis Mary KhoshbeenBoudal | 2006 | Cancer Genetics and Cytogenetics2006，169，1： | 1 |
| 12 | Ivermectin treatment of a traveler who returned from peru with cutaneous gnathostomiasis显示文摘 | Chappuis F Farinelli T Loutan L | 2001 | Clin Infect Dis2001，33，15： | 1 |
| 13 | Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer 显示文摘 | Foulkes W D Stefansson I M Chappuis P O | 2003 | J Natl Cancer Inst2003，95，： | 1 |
| 14 | Proteome analysis of Aeetobacter pasteurianus during acetic acid fermentation显示文摘 | Cristina Andr6s-Barrao Maged M Saad Marie-Louise Chappuis | 2012 | Joumal of proteomics2012，75，6： | 1 |
| 15 | Germline BRCA1 mutations and a basal epithelial phenotype in breast cancer显示文摘 | Foulkes WD Stefansson IM Chappuis PO | 2003 | J Natl Cancer Inst2003，95，： | 1 |
| 16 | Aortic dissection as complication of percutaneous transluminal coronary angioplasty显示文摘 | Moles VP Chappuis F Simonet F | 1992 | Cathet Cardiovasc Diagn1992，26，1： | 1 |
| 17 | Aortic dissection as complication of percutaneous transluminal coronary angioplasty显示文摘 | MOLES V P CHAPPUIS F SIMONET F | 1992 | Cathet Cardiovasc Diagn1992，26，1： | 1 |
| 18 | Germline BRCA1 mutations and a basal epit helial phenotype in breast cancer显示文摘 | Foulkes WD Stefansson IM Chappuis PO | 2003 | J Natl Cancer Inst2003，95，19： | 1 |
| 19 | The intestinal immune system and its relation to disease显示文摘 | LAISSUE J A CHAPPUIS B B MUKLLER C | 1993 | Dig Dis1993，11，： | 1 |
| 20 | Biomechanical evalua- tion of a novel fenestrated pedicle screw augmented with bone cement in osteoporotic spines显示文摘 | Pare PE Chappuis JL Rampersaud R | 2011 | Spine (Phila Pa 1976)2011，18，： | 1 |
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