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| 1 | SEQUENCE VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL144 OPEN READING FRAME IN LOW-PASSAGE CLINICAL ISOLATES显示文摘Objective To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease. Methods HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 lowpassage isolates [65 con-genitally infective children and 7 healthy children who were HCMV-DNA positive by quantitative PCR (qPCR)]. All positive PCR products were analyzed by heteroduplex mobility assay and single-stranded conformation polymorphism (HMA-SSCP) and 32 of them were sequenced. Results Fifty-five patient isolates and five healthy children isolates were HCMV-UL144 positive by PCR. Sequencing and HMA-SSCP analysis showed that significant strain-specific variability was present in the UL144 ORF. Phylogenetic analysis indicated that the nucleotide sequences could be separated into 3 major genotypes. Comparing between UL144 se-quences and the corresponding symptoms showed that genotype 2 did not exist in megacolon isolates. And genotype 1 and 3 were the major types among microcephaly and jaundice isolates respectively. Conclusions HCMV-UL144 existed in most of low passage isolates and sequences were hypervariable. The UL144 ORF and its predicted product with the high level of sequence variability in different kinds of isolates suggest that UL144 ORF might play a role in HCMV infectivity and subsequent diseases. | RongHe QiangRuan ChangXia Lan-qingLiu Sheng-minLǔ YingLu YingQi Yan-pingMa QingLiu Yao-huaJi | 2004 | Chinese Medical Sciences Journal2004，19，4： | 6 |
| 2 | Three New Complexes Based on the Flexible Zwitterionic Dicarboxylate Ligand: Synthesis, Structures, and Properties显示文摘三新建筑群基于转变金属和两性离子的 dicarboxylate ligand 被准备了 1,1 ′-(1,4-phenylene-bis (甲又))-bis(pyridine-3-carboxylic 酸)(L) ,其公式是 {[Cd 4(L)(SO4)]·(Cl )6} n (1 ) ,[Zn (L) Cl 2] n (2 ) ,并且[公司(L) Cl 2] n (3 ) 分别地。建筑群 1-3 被元素的分析,红外光谱学, XRD,热分析和单身者描绘了水晶 X 光检查(粉末) 衍射。1 在单斜晶的空间组 C2/m 结晶,显示有三个唯一的 Cd 2+ 中心的 2-D 层结构。2 和 3 是与斜方晶的 Cmca 空间组一起的同形的结构。他们展出 1-D 之字形链,它能被连接经由氢结合相互作用(CH···O ) 产生 2-D 层。而且,电气化学的行为表明 3 用户终端设备的氧化还原作用反应是控制表面的反应。并且标题建筑群的光性质在房间温度被调查了。 | Qing Wang Bao Mu Dandan Yang Lei Lv Changxia Li Rudan Huang | 2016 | Chinese Journal of Chemistry2016，34，2： | 1 |
| 3 | Fluorimetric determination of ascorbic acid with o-phenylenediamine显示文摘 | Xia Wu Yuxia Diao Changxia Sun | 2003 | Talanta2003，59，1： | 1 |
| 4 | Addition of Al-Ti-C master alloys and ZrO2 to improve the performance of alumina matrix ceramic materials 显示文摘 | LIU Changxia ZHANG Jianhua ZHANG Xihua | 2006 | Mater Sci Eng A2006，426，12： | 1 |
| 5 | Addition of Al- TiC master alloys and ZrO2 to improve the performance of alumina matrix ceramic materials显示文摘 | Liu ChangXia Zhang Jianhua Zhang Xihua | 2006 | Materials Science and Engineering2006，426，： | 1 |
| 6 | The Fluorescence Enhancement of the Protein Adsorbed on the Surface of Ag Nanopartiele显示文摘 | Sun Changxia Wu Xia Ding Honghong | 2009 | Fluorese2009，，19： | 1 |
| 7 | Pressureless sintering of large-scale fine structural alumina matrix ceramic guideway materials 显示文摘 | LIU Changxia ZHANG Jianhua SUN Junlong | 2007 | Mater Sci EngA2007，444，12： | 1 |
| 8 | Ag island film-enhanced rare earth co-luminescence effect of Tb-Gd-pro-tein-sodium dodecyl benzene sulfonate system and sensitive detection of protein 显示文摘 | Sun Changxia Wu Xia Zhou Haiping? | 2008 | Talanta2008，76，5： | 1 |
| 9 | Patients with arteriovenous fistula in hemodialysis nursing显示文摘 | Zhang Changxia | 2014 | Chinese convalescent medicine2014，08，15： | 1 |
| 10 | The fluorescence enhancement effect of Tb–Gd–adenosine triphosphate–phen system and its analytical application显示文摘 | Lei Li Jinghe Yang Xia Wu Changxia Sun Yang Liu Shufang Liu Benyu Su | 2004 | Talanta2004，，1： | 1 |
| 11 | Flow inj ection-chemil determination of amoxicillin using potassium permanganate and formaldehyde system显示文摘 | Cao Wei Yang Jinghe Sun Changxia | 2005 | Luminescence2005，20，1： | 1 |
| 12 | 显示文摘 | Liu Changxia Zhang Jianhua Zhang Xihua | 2007 | Materials Science and Engineering A2007，465，： | 1 |
| 13 | Fabrication of AI2O3/TiB2/AIN/TiN and AI2O3/TiC/AIN composites显示文摘 | Liu Changxia Zhang Jianhua Zhang Xihua | 2007 | Materials Science and Engineering:A2007，465，12： | 1 |
| 14 | miR-21 and miR-101 regulate PLAP-1 expression in periodontal ligamentcells显示文摘 | Changxia Li Chunlei Li Jing Yue Xin Huang Meimei Chen Jie Gao Buling Wu | 2012 | Molecular Medicine Reports2012，，5： | 1 |
| 15 | Toughening Mechanisms of Alumina Matrix Ceramic Composite Toughened by Rare Earth Additive显示文摘 | Zhang Xihua Liu Changxia Li Musen Zhang Jianhua | 2006 | Journal of Rare Earths2006，24，4： | 0 |
| 16 | EVIDENCE OF PLUMB LINE VARIATIONS IN EUROPE FROM LATITUDE OBSERVATIONS显示文摘In response to the resolutions of the IUGG-IAG and IAU in 1991, a study of thepossibility of deriving long-term variations of bldnib line from Optical astrometric latitudedata has been carried out. Evidence of the existence of plumb line variations in Europe,ofwhich the periods are about 4 to 6 years, will be sh0wn in the | Li Zhengxin Qiah Changxia(Shanghai Observatory, Chinese Academy of Sciences) | 1995 | 中国科学院上海天文台年刊1995，，16： | 0 |
| 17 | Short Group Signatures with Efficient Concurrent Join显示文摘Group signature schemes are fundamental cryptographic tools.A group signature scheme allows members of a group to anonymously sign messages.To counter misuse,the anonymity can be revoked by the group manager.The group joining operation is a critical component of group signature scheme,the framing attack can be prevented by group joining processes.This paper presents an efficient group signature scheme with a simple joining protocol that is based on a 'single message and signature response' interaction between the prospective user and the group manager.The security of our group signature is based on the Discrete Logarithm assumption and Decisional Linear DiffieHellman assumption.The formal security proof of our scheme is given in the random oracle model.Our scheme is also a very efficient short group signature scheme with efficient concurrent join. | CHEN Hefeng MA Wenping ZHANG Cheng SUN Changxia | 2014 | China Communications2014，11，11： | 0 |
| 18 | Five Steps to Cultivate Volvariella volvacea显示文摘Volvariella volvacea is a popular variety because of its distinct flavor, pleasant taste, higher protein content and shorter cropping duration compared with other cultivated mushrooms. It is also the most expensive mushroom in artificial mushrooms.With the development of economy and the industrialization of edible fungi, more and more straw mushroom has been cultivated in northern China, and southern mushroom is moving to northern areas. In this study, based on many years of cultivation experience, the high yield cultivating techniques of V. volvacea was summarized. V. volvacea cultivation consists of five steps, i.e.,composting, pasteurization, spawning, pining and harvesting. | Zhengpeng LI Mingjie CHEN Changxia YU Qiaozhen LI Feng ZHOU Yu LI | 2017 | Agricultural Science & Technology2017，18，9： | 0 |
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