2篇
您的检索式：作者名="Changlei Feng"
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| 1 | SARS-CoV-2 spike protein receptor-binding domain perturbates intracellular calcium homeostasis and impairs pulmonary vascular endothelial cells显示文摘Exposure to the spike protein or receptor-binding domain(S-RBD)of SARS-CoV-2 significantly influences endothelial cells and induces pulmonary vascular endotheliopathy.In this study,angiotensin-converting enzyme 2 humanized inbred(hACE2 Tg)mice and cultured pulmonary vascular endothelial cells were used to investigate how spike protein/S-RBD impacts pulmonary vascular endothelium.Results show that S-RBD leads to acute-to-prolonged induction of the intracellular free calcium concentration([Ca^(2+)]i)via acute activation of TRPV4,and prolonged upregulation of mechanosensitive channel Piezo1 and store-operated calcium channel(SOCC)key component Orai1 in cultured human pulmonary arterial endothelial cells(PAECs).In mechanism,S-RBD interacts with ACE2 to induce formation of clusters involving Orai1,Piezo1 and TRPC1,facilitate the channel activation of Piezo1 and SOCC,and lead to elevated apoptosis.These effects are blocked by Kobophenol A,which inhibits the binding between S-RBD and ACE2,or intracellular calcium chelator,BAPTA-AM.Blockade of Piezo1 and SOCC by GsMTx4 effectively protects the S-RBDinduced pulmonary microvascular endothelial damage in hACE2 Tg mice via normalizing the elevated[Ca^(2+)]i.Comparing to prototypic strain,Omicron variants(BA.5.2 and XBB)of S-RBD induces significantly less severe cell apoptosis.Transcriptomic analysis indicates that prototypic S-RBD confers more severe acute impacts than Delta or Lambda S-RBD.In summary,this study provides compelling evidence that S-RBD could induce persistent pulmonary vascular endothelial damage by binding to ACE2 and triggering[Ca^(2+)]i through upregulation of Piezo1 and Orai1.Targeted inhibition of ACE2-Piezo1/SOCC-[Ca^(2+)]i axis proves a powerful strategy to treat S-RBD-induced pulmonary vascular diseases. | Kai Yang Shiyun Liu Han Yan Wenju Lu Xiaoqian Shan Haixia Chen Changlei Bao Huazhuo Feng Jing Liao Shuxin Liang Lei Xu Haiyang Tang Jason X-J.Yuan Nanshan Zhong Jian Wang | 2023 | Signal Transduction and Targeted Therapy2023，8，8： | 2 |
| 2 | Flexible thermoelectric generator and energy management electronics powered by body heat显示文摘Uninterrupted,efficient power supplies have posed a significant hurdle to the ubiquitous adoption of wearable devices,despite their potential for revolutionizing human‒machine interactions.This challenge is further compounded by the requirement of these devices to supply dependable energy for data-intensive sensing and transmission.Traditional thermoelectric solutions fail to deliver satisfactory performance under conditions of extremely low voltages.Here,we present a novel solution of a wearable thermoelectric generator integrated with an energy management system,which is capable of powering sensors and Bluetooth by harnessing body heat.Distinct from previous works,our innovation lies in its ability to consistently operate even with a minimal temperature difference(i.e.,4 K)between the human skin and the ambient environment,ensuring reliable data transmission within a time as short as 1.6 s.Furthermore,our system can recharge utilizing body heat under ultralow voltage conditions(30 mV).Our developed system provides a novel pathway for the continuous,reliable monitoring of self-contained wearable devices without depending on batteries. | Shuai Yang Yumei Li Ling Deng Song Tian Ye Yao Fan Yang Changlei Feng Jun Dai Ping Wang Mingyuan Gao | 2023 | Microsystems & Nanoengineering2023，9，4： | 0 |
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