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| 1 | Recent advances in nanorobotic manipulation inside scanning electron microscopes显示文摘A scanning electron microscope(SEM)provides real-time imaging with nanometer resolution and a large scanning area,which enables the development and integration of robotic nanomanipulation systems inside a vacuum chamber to realize simultaneous imaging and direct interactions with nanoscaled samples.Emerging techniques for nanorobotic manipulation during SEM imaging enable the characterization of nanomaterials and nanostructures and the prototyping/assembly of nanodevices.This paper presents a comprehensive survey of recent advances in nanorobotic manipulation,including the development of nanomanipulation platforms,tools,changeable toolboxes,sensing units,control strategies,electron beam-induced deposition approaches,automation techniques,and nanomanipulation-enabled applications and discoveries.The limitations of the existing technologies and prospects for new technologies are also discussed. | Chaoyang Shi Devin K Luu Qinmin Yang Jun Liu Jun Chen Changhai Ru Shaorong Xie Jun Luo Ji Ge Yu Sun | 2016 | Microsystems & Nanoengineering2016，2，1： | 4 |
| 2 | Automated four-point probe measurement of nanowires inside a scanning electron microscope 显示文摘 | Ru Changhai Zhang Yong Sun Yu | 2011 | IEEE Transactions on N anotechnolugy2011，10，4： | 1 |
| 3 | A new amplifier for improving piezoelectric actuator linearity based on current switching in precision positioning显示文摘 | RU Changhai CHEN Liguo SHAO Bing | 2008 | Meas Sci Technol2008，19，1： | 1 |
| 4 | A spiral microfluidic device for rapid sorting,trapping,and long-term live imaging of Caenorhabditis elegans embryos显示文摘Caenorhabditis elegans embryos have been widely used to study cellular processes and developmental regulation at early stages.However,most existing microfluidic devices focus on the studies of larval or adult worms rather than embryos.To accurately study the real-time dynamics of embryonic development under different conditions,many technical barriers must be overcome;these can include single-embryo sorting and immobilization,precise control of the experimental environment,and long-term live imaging of embryos.This paper reports a spiral microfluidic device for effective sorting,trapping,and long-term live imaging of single C.elegans embryos under precisely controlled experimental conditions.The device successfully sorts embryos from a mixed population of C.elegans at different developmental stages via Dean vortices generated inside a spiral microchannel and traps the sorted embryos at single-cell resolution through hydrodynamic traps on the sidewall of the spiral channel for long-term imaging.Through the well-controlled microenvironment inside the microfluidic device,the response of the trapped C.elegans embryos to mechanical and chemical stimulation can be quantitatively measured.The experimental results show that a gentle hydrodynamic force would induce faster growth of embryos,and embryos developmentally arrested in the high-salinity solution could be rescued by the M9 buffer.The microfluidic device provides new avenues for easy,rapid,high-content screening of C.elegans embryos. | Peng Pan Zhen Qin William Sun Yuxiao Zhou Shaojia Wang Pengfei Song Yong Wang Changhai Ru Xin Wang John Calarco Xinyu Liu | 2023 | Microsystems & Nanoengineering2023，9，1： | 0 |
| 5 | The m6A reader MhYTP2 regulates the stability of its target mRNAs contributing to low nitrogen tolerance in apple (Malus domestica)显示文摘Studies have shown that the m6A reader primarily affects genes expression by participating in the regulation of mRNA localization,splicing,degradation,translation,and other metabolic processes.Previously,we discovered that the apple(Malus domestica)m6A reader MhYTP2 bound with and destabilized m6A-modified MdMLO19 mRNA.In addition,it enhanced the translation efficiency of m6A-modified mRNA of MdGDH1L,encoding a glutamate dehydrogenase,which confers resistance to powdery mildew.In this study,we report the function of MhYTP2 in the regulation of resistance to low nitrogen(N).The overexpression of MhYTP2 enhances the resistance of apple to low N.We show that MhYTP2 binds with and stabilizes the mRNAs of MdALN,which participates in the allantoin catabolic process and cellular response to N starvation in apple;MdPIDL,which participates in root hair elongation;MdTTG1,which is involved in the differentiation process of trichomes;and MdATG8A,which is a core participant in the regulation of autophagy.In addition,MhYTP2 accelerates the degradation of MdRHD3 mRNA,which regulates root development.RNA immunoprecipitation-seq and electrophoretic mobility shift assays show that the mRNAs of MdALN,MdATG8A,MdPIDL,MdTTG1,and MdRHD3 are the direct targets of MhYTP2.Overexpressing or knocking down the above genes in MhYTP2 overexpressing plants dismisses the function of MhYTP2 under low N,suggesting the role of MhYTP2 is dependent on those genes.Together,these results demonstrate that MhYTP2 enhances the resistance of apple to N deficiency by affecting the stability of the bound mRNAs. | Tianli Guo Zehua Yang Ru Bao Xiaomin Fu Na Wang Changhai Liu Fengwang Ma | 2023 | Horticulture Research2023，10，6： | 0 |
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