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| 1 | Increasing the frequency of CIK cells adoptive immunotherapy may decrease risk of death in gastric cancer patients显示文摘AIM: To analyze the correlation between cytokineinduced killer (CIK) cells adoptive immunotherapy and cancer-related death in gastric cancer patients. METHODS: One hundred and fifty-six gastric cancer patients after operation at the Third Affiliated Hospital of Soochow University were enrolled in this study. Their clinical data including demographic characteristics, operation time, tumor size, pathological type and staging, tumor metastasis, outcome of chemotherapy or CIK cells adoptive immunotherapy, survival time or time of death were collected with a standard structured questionnaire. Kaplan-Meier method was used to estimate the median survival time, and the 2- and 5- year survival rates. Hazard risk (HR) and 95% confidence interval (95% CI) of CIK cells adoptive immunotherapy for gastric cancer were calculated using the two-stage time-dependent covariates Cox model. RESULTS: The survival time of gastric cancer patients was longer after CIK cells adoptive immunotherapy than after chemotherapy (χ 2 = 10.907, P = 0.001). The median survival time of gastric cancer patients was also longer after CIK cells adoptive immunotherapy than after chemotherapy (49 mo vs 27 mo, P < 0.05). The 2- and 5-year survival rates of gastric cancer patients were significantly higher after CIK cells adoptive immunotherapy than after chemotherapy (73.5% vs 52.6%, 40.4% vs 23.9%, P < 0.05). A significant difference was observed in the survival curve for patients who received CIK cells adoptive immunotherapy (0, 1-10, 11-25, and over 25 frequencies) (χ 2 = 14.534, P = 0.002). The frequencies of CIK cells adoptive immunotherapy were significantly related with the decreasing risk of death in gastric cancer patients after adjustment for sex and age of the patients, tumor stage and relapse (HR = 0.54, 95% CI: 0.36-0.80) when the first stage Cox model was used to define the subjects who remained alive beyond 36 mo as survivors. However, no correlation was observed between the frequencies of death in CIK cells adoptive immunotherapy and the risk of gastric cancer patients (HR = 1.09, 95% CI: 0.63-0.89) when the second stage Cox model was used to define the subjects who survived for more than 36 mo as survivors. CONCLUSION: The survival time of the gastric cancer patients treated with chemotherapy combined with CIK cells adoptive immunotherapy is significantly longer than that of the patients treated with chemotherapy alone and increasing the frequency of CIK cells adoptive immunotherapy seems to benefit patients more. | Jing-Ting Jiang, Chang-Ping Wu, Lu-Jun Chen, Xiao Zheng, Department of Tumor Biological Treatment, Third Affiliated Hospital of Soochow University, Changzhou 213003, Jiangsu Province, China Yi-Bei Zhu, Jing Sun, Xue-Guang Zhang, Key Laboratory of Stem Cell of Jiangsu Province, Institute of Biotechnology, Key Laboratory of Clinical Immunology of Jiangsu Province, Soochow University, Suzhou 215123, Jiangsu Province, China Yue-Ping Shen, Wen-Xiang Wei, Department of Medicine, Soochow University, Suzhou 215123, Jiangsu Province, China Bin-Feng Lu, Department of Immunology, University of Pitts- burgh School of Medicine, Pittsburgh, PA 15261, United States | 2010 | World Journal of Gastroenterology2010，16，48： | 82 |
| 2 | MAPK signal pathways in the regulation of cell proliferation in mammalian cells显示文摘MAPK families play an important role in complex cellular programs like proliferation, differentiation, development, transformation, and apoptosis. At least three MAPI(families have been characterized: extracellular signal-regulated kinase (ERK), Jun kinase (JNK/SAPK) and p38 MAPK. The above effects are fulfilled by regulation of cell cycle engine and other cell proliferation related proteins. In this paper we discussed their functions and cooperation with other signal pathways in regulation of cell proliferation. | WEI ZHANG, Hui Tu LIU The Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Sciences, Beijing Normal University, Beijing 100875, China | 2002 | Cell Research2002，12，1： | 107 |
| 3 | Growth inhibition and apoptosis induction Sulindac on Human gastric cancer cells显示文摘AIM: To evaluate the effects of sulindac in inducing growth inhibition and apoptosis of human gastric cancer cells in comparison with human hepatocellular carcinoma (HCC)cells.METHODS: The human gastric cancer cell lines MKN45 and MKN28 and human hepatocellular carcinoma cell lines HepG2and SMMC7721 were used for the study. Anti-proliferative effect was measured by MTT assay, and apoptosis was determined by Hoechst-33258 staining, electronography and DNA fragmentation. The protein of cyclooxygenase-2 (COX(2) and Bcl-2 were detected by Westem dot blotting.RESULTS: Sulindac could initiate growth inhibition and apoptosis of MKN45, MKN28, HepG2 and SMMC7721 cells in a dose-and time-dependent manner. Growth inhibitory activity and apoptosis were more sensitive in HepG2 cells than in SMMC7721 cells, MKN45 and MKN28 cells. After 24hours incubation with sulindac at 2mmol. L-1 and 4mmol.L-1, the level of COX-2 and Bcl-2 protein were lowered in MKN45, SMMC7721 and HepG2 cells but not in MKN28 cells.CONCLUSION: Sulindac could inhibit the growth of gastric cancer cells and HCC cells effectively in vitro by apoptosis induction, which was associated with regression of COX-2and Bcl-2 expression. The growth inhibition and apoptosis of HCC cells were greater then that of human gastric cancer cells. The different effects of apoptosis in gastric cancer cells may be related to the differentiation of the cells. | Yun-Lin Wu~1 Bo Sun~1 Xue-Jun Zhang~2 Sheng-Nian Wang~2 Heng-Yi He~2 Min-Min Qiao~1 Jie Zhong~1 Jia-Yu Xu~1 1 Department of Gastroenterology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China2 Institude of Biochemistry and Cell Biology,Shanghai Institues for Biological Sciences,Chinese Academy of Sciences.Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，6： | 64 |
| 4 | Get effective polyclonal antisera in one month显示文摘According to the traditional immunization procedure, after the first injection of the sample A (emulsion of aimed antigen and Freund's complete adjuvant) to immunize rabbit, successive injections of the sample B (emulsion of aimed antigen and Freund's incomplete adjuvant) were followed every 2-4 weeks. In general,high titer of the corresponding polyclonal antisera will be observed after 4-5 injections of sample B in 3-4months. This report presents a simply modified procedure that was able to stimulate the antisera formation in one month and achieve enough avidity to satisfy either Western blot or immunohistochemistry analysis.It just applied an additional injection of the sample A to the rabbit at the 3rd day after the primary immunization injection. You could gain the high titer of the antisera right after the first sample B injection in one month. This method has produced the desired results in three different recombinant antigens with different molecular weight (5.9 KD-55 KD) expressed from prokaryotic or eukaryotic cells. | YUAN XIN HU, Ju YUAN QUO, Lu SHEN, YAN CHEN, Zu CHUAN ZHANG, YONG LIAN ZHANGState Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Yue Yang Road, | 2002 | Cell Research2002，12，2： | 45 |
| 5 | Studies on the integration of hepatitis B virus DNA sequence in human sperm chromosomes显示文摘Aim:To study the integration of hepatitis Bvirus(HBV)DNAinto sperm chromosomes in hepatitsBpatients and the features of its integration.Methods:Sperm chromosomes of 14subjects(5healthy controls and9HBpatients,including1acute hepatitis B,2chronic active hepatitisB,4chronic persistent hepatitsB,2HBsAg chronic carriers with no clinical symptoms)were prepared using imterspecific in vitro fertilization between zona-free hamster oocytes and human spermatozoa.Fluosescence in situ hybridization(FISH)to sperm chromosome spreads was carried out with biotin-labeled full length HBVDNAprobe to detect the specificHBVDNA sequences in the sperm chromosomes.Results:Specific fluorescent signal spots for HBVDNAwere seen iv sperm chromosomes of one patient with chronic persistent hepatitisB.In9(9/42)sperm chromosome complements containing fluorescent signal spots,one presented5obvious FISHspots and the others2to4signals.The fluorescence intensity showed significant difference among the signal spots.The distribution of signal sites among chromosomes seems to be random.Con clusion:HBV could integrate into human sperm chromosomes.Results suggest that the possibility of vertical transmission of HBVvia the germ line tothe next generation is present. | Jian-Min HUANG~(1,*), Tian-Hua HUANG~1, Huan-Ying QIU~2, Xiao-Wu FANG~1, Tian-Gang ZHUANG~1, Jie-Wen QIU~3 ~1Department of Cell Biology and Medical Genetics, Shantou University Medical College ~2Shantou University Hospital ~3Department of Infectious Disease of the Second Affiliated Hospital, Shantou University Medical College, Shantou 515031, China | 2002 | Asian Journal of Andrology2002，4，3： | 49 |
| 6 | Targeting Gene-Virotherapy of Cancer and its prosperity显示文摘为癌症的基因和病毒的治疗显示出一些治疗学的效果,但是有真实突破的缺乏。为了达到肿瘤的完全的消除的目标,在动物的异种皮移植当模特儿,我们开发了叫的新策略指向癌症的 Gene-Virotherapy,它试图联合基因治疗和 virotherapy 的优点。这新策略独自比基因或病毒的治疗生产了更强壮的反肿瘤效果。肿瘤特定的 replicative 侵入人体气管粘膜的病菌向量,指定了为 ZD55,被侵入人体气管粘膜的病菌的 55kDa E1B 区域的删除构造。结果病毒的构造不仅由明确地指向 p53 保留类似的功能到 ONYX-015 否定肿瘤,而且允许各种各样的治疗学的基因到的插入由于最新介绍的克隆的地点形成适当 ZD55 衍生物,与原来的 ONYX-015 病毒不可行的一项任务。我们证明反肿瘤一如此的衍生物完成, ZD55-IL-24,比 ZD55 virotherapy 或 Ad-IL-24 基因治疗的是乘更多的有势力的至少 100。不过,由 ZD55-IL-24 的使用的肿瘤质量的完全的消除是仅仅在一些然而并非所有老鼠观察了,显示那一治疗学的基因不对“痊愈”足够这些老鼠。有互补或合作的效果的基因是什么时候,独立克隆进 ZD55 向量并且在联合使用了(指定了为双基因治疗策略) ,好一些的结果被获得;并且如果二合适的基因被选择,在所有老鼠完成所有异种皮移植肿瘤群众的完全的消除是可能的。更全面的研究将多半为临床的试用基于这新策略导致一个协议。最后,为癌症治疗的两倍控制指向的病毒双的基因治疗的概念,和在癌症干细胞的最近的进步的含意也被讨论。 | Xin Yuan Liu~(1,2) ~1Institute of Biochemistry and Cell Biology,Shanghai Institutes for Biological Sciences,Chinese Academy of Sciences,320 Yue Yang Road,Shanghai 200031,China ~2Xinyuan Institute of Medicine and Biotechnology,School of Life Science,Zhejiang Sci-Tech University,Hangzhou 310018,China | 2006 | Cell Research2006，16，11： | 32 |
| 7 | Specific CEA-producing colorectal carcinoma cell killing with recombinant adenoviral vector containing cytosine deaminase gene显示文摘瞄准:到杀死 CEA 积极颜色明确地使用 E 关口 i cytosine 脱氨基酶(CD ) 的表面的癌房间自杀基因,新复制缺乏的 recombinant adenoviral 向量在在哪个 CD,基因在 CEA 倡导者下面被控制被构造并且它的在里面 vitro 细胞毒素的效果被评估。方法:梭原生质标志包含 CD 基因和 CEA 基因的规章的顺序在 293 房间紧张与侵入人体气管粘膜的病菌染色体 DNA 的右手臂被构造并且重新结合。点弄污, PCR 被用来识别积极的匾。侵入人体气管粘膜的病菌的纯化被执行与在 CsCl 步坡度和滴定极端集中与匾形成试金被测量。细胞毒素的效果是有 MTT 方法的 assayed, 5-FC 的百分之五十抑制集中(IC (50 )) 用一个曲线试穿参数被计算。表面的癌房间线,是生产 CEA,和 CEA-nonproducing Hela 房间衬里的人的颜色在 cytological 测试被使用。确定的 recombinant 侵入人体气管粘膜的病菌向量 AdCMVCD, CD 基因在 CMV 倡导者下面在被控制,被用作病毒控制。量的结果被表示为平均数的吝啬的 +/- SD。统计分析用 ANOVA 测试被执行。结果:需要的 recombinant 侵入人体气管粘膜的病菌向量被称为 AdCEACD。点弄污和 PCR 的结果证明 recombinant 侵入人体气管粘膜的病菌包含了 CEA 倡导者和 CD 基因。病毒 titer 是大约 5.0 X 10 (14 ) pfu/L (在纯化以后的 -1) 。 生产CEA Lovo 房间对 5-FC 敏感并且与 AdCEACD 和 AdCMVCD 在感染以后有一样的细胞毒素的效果(在父母 Lovo 房间,感染 100 M.O.I AdCEACD 的 Lovo 房间和感染 10 M.O.I AdCMVCD 的 Lovo 房间的 5-FC 的 IC ( 50 )价值是 >15000 , 216.5+/-38.1 和 128.8+/-25.4 micromol.L (-1), P<0.001 ,分别地),并且当侵入人体气管粘膜的病菌的 m.o.i 被提高时, 5-FC 的 cytotoxicity 因此增加了( 5-FC 的 IC ( 50 )的价值被归结为 27.9+/-4.2 micromol.L (在 1000 M.O.I AdCEACD 的-1)感染了 Lovo 房间和 24.8+/-7.1 micromol.L (在 100 M.O.I AdCMVCD 的-1)感染了 Lovo 房间, P<0.05 , P<0.01 ,分别地)。CEA-nonproducing Hela 房间没与 AdCEACD 在感染以后有效果,但是 Hela 房间与 AdCMVCD 在感染以后有细胞毒素的敏感到 5-FC (在在 10 M.O.I 感染 AdCMVCD 的父母 Hele 房间和 Hela 房间的 5-FC 的 IC (50 ) 是 >15000 和 214.5+/-31.3 micromol.L (-1), P<0.001 ) 。AdCEACD/5-FC 系统也有旁观者效果,并且当 transfected 房间的比例仅仅是 10% 时,生存能力是大约 30% 。结论:recombinant 侵入人体气管粘膜的病菌向量 AdCEACD 有房间的特性类型特定的基因交货。AdCEACD/5-FC 系统可以变得一新,为 CEA 积极的瘤的基因治疗的有势力和特定的途径,特别结肠癌。 | Li-Zong Shen Wen-Xi Wu Qiang Ding Yi-Bing Hua,Department of General Surgery,The First Affiliated Hospital of Nanjing Medical University,Nanjing,210029,Jiangsu Province,China De-Hua Xu Zhong-Cheng Zheng Xin-Yuan Liu,Shanghai Institute of Biochemistry and Cell Biology,The Chinese Academy of Sciences,Shanghai,200031,China Kun Yao,Department of Microbiology and Immunology,Nanjing Medical University,Nanjing,210029,Jiangsu Province,China | 2002 | World Journal of Gastroenterology2002，8，2： | 29 |
| 8 | Intergrin gene expression profiles of human hepatocellular carcinoma显示文摘AIM: To investigate gene expression profiles of intergringenes in hepatocellular carcinoma (HCC) through theusage of Atlas Human Cancer Array membranes, semi-quantitative reverse transcription polymerase chainreaction (RT-PCR) and Northern blot.METHODS: Hybridization of cDNA array membrane wasperformed with α 32P-labeled cDNA probes synthesizedfrom RNA isolated from hepatocellular carcinoma andadjacent non-cirrhotic liver. AtlasImage, which is asoftware specific to array, was used to analyze theresult. RT-PCR of 24 pairs specimen and Northern blotof 4 pairs specimen were used to confirm the expressionpattern of some intergrin genes identified by Atlasarrays hybridization.RESULTS: Among 588 genes spotted in membrane, 17genes were related to intergrin. Four genes were up-regulated, such as intergrin alpha8, beta1, beta7 andbeta8 in HCC. Whereas there were no genes down-regulated in HCC. RT-PCR and Northern blot analysisof intergrin beta1 gene gave results consistent withcDNA array findings.CONCLUSION: Investigation of these intergrin genesshould help to disclose the molecular mechanism of thecell adhesion, invasive and metastasis of HCC. A fewgenes are reported to have changed in HCC for the firsttime. The quick and high-throughout method of profilinggene expression by cDNA array provides us overviewof key factors that may involved in HCC, and may findthe clue of the study of HCC metastasis and moleculartargets of anti-metastasis therapy. The preciserelationship between the altered genes and HCC is amatter of further investigation. | Lian-Xin Liu Hong-Chi Jiang Wei-Hui Zhang An-Long Zhu Department of Surgery,the First Clinical College,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Lian-Xin Liu Zhi-Hua Liu Jing Zhou Xiu-Qin Wang Min Wu National Laboratory of Molecular Oncology,Department of Cell Biology,Cancer Institute,Chinese Academy of Medical Science & Peking Union Medical College,Beijing 100021,China | 2002 | World Journal of Gastroenterology2002，8，4： | 29 |
| 9 | Discovery of laryngeal carcinoma by serum proteomic pattern analysis显示文摘Laryngeal carcinoma is the most common malignancy among head and neck tu-mors. The purpose of this study is to find biomarkers for laryngeal carcinoma in patient blood serum using the Surface Enhanced Laser Desorption/Ionization (SELDI) technique. Serum samples from 33 laryngeal carcinoma (12 cases of glottis, 18 of supraglottis and 3 of subglottis) patients and 31 age- and sex-matched healthy people were analyzed by SELDI-TOF on a Pro-teinChip reader, PBSII-C. Protein profiles were generated using WCX2 protein chips. Protein peak clustering and classification analyses were performed utilizing the Biomarker Wizard and Biomarker Pattern software packages, respectively. The results showed that sixteen peaks had significant difference between laryngeal cancer patients and healthy group, eight of which were up-regulated in the patient samples, and the others were down-regulated. Two protein peaks 8153 Da and 2035 Da were automatically chosen for the system training and development of a classification tree. The analysis yielded a correct percentage of 96.9% for patients and 96.7% for control. The results suggest that serum is a useful resource for the detection of specific bio-markers for laryngeal carcinoma. Proteinchip Array System was a useful tool for a high throughput screening of large-sized serum samples to discover potential biomarkers for carci-noma. | XIAO Xueyuan1, ZHAO Xiaodong2, LIU Jiankai3, GUO Fuzheng1, LIU Danhui1 & HE Dacheng1 1. Key Laboratory for Cell Proliferation and Regulation Biology, Ministry of Education, Beijing Normal University, Universities?Confeder-ated Institute of Proteomics, Beijing 100875, China 2. Department of Otolaryngology-Head and Neck Surgery, Third Affiliated Hospital, Jilin University, Changchun 130031, China 3. Department of Biochemistry, Jilin University, Changchun 130021, China | 2004 | Science China(Life Sciences)2004，47，3： | 22 |
| 10 | Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro显示文摘AIM: To study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma (HCC) cells in vitro.METHODS: Antisense RNA of HAb18G/CD147 vector PCIasHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immunohistochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in vitro.RESULTS: The construction of antisense RNA vector PCIasHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC,respectively.CONCLUSION: Invasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC. | Yu Li Peng Shang Ai-Rong Qian Li Wang Yong Yang Zhi-Nan Chen, Department of Cell Biology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China | 2003 | World Journal of Gastroenterology2003，9，10： | 22 |
| 11 | p16 gene methylation in colorectal cancers associated with Duke's staging显示文摘AIM To explore the association of methylation of the CpG island in the promotor of the p16 tumor suppressor gene with the clinicopathological characteristics of the colorectal cancers.``METHODS Methylation-specific PCR (MSP) was used to detect p16 methylation of 62 sporadic colorectal cancer specimens.``RESULTS pi6 methylation was detected in 42% of the tumors. Dukes staging was associated with p16methylation status, p16 methylation occurred more frequently in Dukes C and D patients (75.9%) than in Dukes A and B patients (12.1%).``CONCLUSION p16 rnethylation plays a role in the carcinogenesis of a subset of colorectal cancer, and it might be linked to poor prognosis. | Jing Yi~1 Zhi-Wei Wang~1 Hui Cang~1 Yu-Ying Chen~1 Ren Zhao~2 Bao-Ming Yu~2 Xue-Ming Tang~1 1 Department of Cell Biology,2 Department of Surgery,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China | 2001 | World Journal of Gastroenterology2001，7，5： | 21 |
| 12 | A Genome-wide Functional Characterization of Arabidopsis Regulatory Calcium Sensors in Pollen Tubes显示文摘Calcium, an ubiquitous second messenger, plays an essential and versatile role in cellular signaling. The diverse function of calcium signals is achieved by an excess of calcium sensors. Plants possess large numbers of calcium sensors, most of which have not been functionally characterized. To identify physiologically relevant calcium sensors in a specific cell type, we conducted a genome-wide functional survey in pollen tubes, for which spatiotemporal calcium signals are well-characterized and required for polarized tip growth. Pollen-specific members of calmodulin (CaM), CaM-like (CML), calcium-dependent protein kinase (CDPK) and calcineurin B-like protein (CBL) families were tagged with green fluorescence protein (GFP) and their localization patterns and overexpression phenotypes were characterized in tobacco pollen tubes. We found that several fusion proteins showed distinct overexpression phenotypes and subcellular localization patterns. CDPK24-GFP was localized to the vegetative nucleus and the generative cell/sperms. CDPK32-GFP caused severe growth depolarization. CBL2-GFP and CBL3-GFP exhibited dynamic patterns of subcellular localization, including several endomembrane compartments, the apical plasma membrane (PM), and cytoskeleton-like structures in pollen tubes. Their overexpression also inhibited pollen tube elongation and induced growth depolarization. These putative calcium sensors are excellent candidates for the calcium sensors responsible for the regulation of calcium homeostasis and calciumdependent tip growth and growth oscillation in pollen tubes. | Liming Zhou1,2, Ying Fu1,2 and Zhenbiao Yang2,3 (1State Key Laboratory of Plant Physiology and Biochemistry, Department of Plant Sciences, College of Biological Sciences, China Agricultural University, Beijing 100193, China 2China Agricultural University (CAU)-University of California, Riverside (UCR) Joint Center for Biological Sciences and Biotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China 3Center for Plant Cell Biology and Department of Botany and Plant Sciences, University of California, Riverside, CA 92521, USA) | 2009 | Journal of Integrative Plant Biology2009，51，8： | 21 |
| 13 | Antihepatoma effect of alpha-fetoprotein antisense phosphorothioate oligodeoxyribonucleotides in vitro and in mice显示文摘AIM To evaluate antihepatoma effect ofantisense phosphorothioate oligodeo-xyribonucleotides (S-ODNs) targeted to alpha-fetoprotein (AFP) genes in vitro and in nudemice.METHODS AFP gene expression was examinedby immunocytochemical method or enzyme-linked immunosorbent assay. Effect of S-ODNson SMMC-7721 human hepatoma cell growth invitro was determined using microculturetetrazolium assay. In vivo antitumor activitiesof S-ODNs were monitored by measuring tumorweight differences in treated and control micebearing SMMC-7721 xenografts. Induction of cellapoptosis was evaluated by fluorescence-activated cell sorter (FACS) analysis.RESULTS Antisense S-ODN treatment led toreduced AFP gene expression. Specificantisense S-ODNs, but not control S-ODNs,inhibited the growth of heaptoma cells in vitro.In vivo. only antisense S-ODNs exhibitedobvious antitumor activities. FACS analysisrevealed that the growth inhibition by antisenseS. ODNs was associated with their cell apoptosisinduction.CONCLUSION Antisense S-ODNs targeted toAFP genes inhibit the growth of human hepatomacells and solid hepatoma, which is related totheir cell apoptosis induction. | Xing Wang Wang~1 Jin Hui Yuan~1 Ru Gang Zhang~1 Li Xia Guo~1 Yong Xie~2 Hong Xie~1 ~1Department of Biotherapy,Shanghai Institute of Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China ~2Department of Biology,Hong Kong University of Science and Technology,ChinaDr.Xing Wang Wang earned Ph.D.from Shanghai Institute of Materia Medical,Chinese Academy of Sciences in 1997.Now a professor at Shanghai Institute of Cell Biology,Chinese Academy of Sciences. | 2001 | World Journal of Gastroenterology2001，7，3： | 21 |
| 14 | Serum thymosin β4 levels in patients with hepatitis B virus-related liver failure显示文摘AIM:To investigate whether serum thymosinβ4 can provide diagnostic or prognostic information in liver failure patients caused by chronic hepatitis B virus(HBV) infection. METHODS:Serum thymosinβ4 levels were measured in 30 patients with acute-on-chronic liver failure(ACLF), 31 patients with chronic liver failure(CLF),30 patients with compensated liver cirrhosis(CR)and 32 patients with chronic hepatitis B and 30 healthy controls.Serum thymosinβ4 levels were measured by enzyme-linked immunosorbent assay and Child-Pugh and model for end-stage liver disease(MELD)scores were calculated for each patient on admission.RESULTS:Compared with healthy controls,serum thymosinβ4 levels in ACLF,CLF,CR and chronic hepatitis B patients were significantly lower,6.5047 (4.7879-10.5314)μg/mL vs 0.4632(0.2759-0.8768) μg/mL,0.6981(0.5209-1.2008)μg/mL,1.8053 (0.8110-2.3397)μg/mL,3.7803(1.8570-6.4722)μg/mL, respectively(P<0.001).The levels of thymosinβ4 in liver failure(ACLF or CLF)patients were markedly lower than that in CR(P<0.001),and a difference was also found between CLF and ACLF patients(P=0.038).In patients with chronic liver disease,there was a positive relationship between thymosinβ4 levels and albumin, choline esterase,and platelet(P<0.001),and negative relationship with alanine aminotransferase(P=0.020), aspartate aminotransferase,total bilirubin,international normalized ratio of prothrombin time,and Child-Pugh and MELD scores(P<0.001).Of the 61 liver failure patients,the thymosinβ4 levels of non-survivors were significantly lower than that of survivors(P=0.007). Receiver operating characteristics analysis identified a thymosinβ4 cutoff level of 0.5708μg/mL for predicting poor prognosis in all liver failure patients.The serial thymosinβ4 values were observed in 13 liver failure inpatients.Lower initial values were observed in the death.While greater improvement in thymosinβ4 value was found in those who recovered from the disease. CONCLUSION:Serum thymosinβ4 can be used as an important potential predictor for liver failure caused by chronic HBV infection. | Tao Han,Ying Liu,Huan Liu,Zheng-Yan Zhu,Yan Li,Shi-Xiang Xiao,Zhen Guo,Zhen-Gang Zhao,Department of Hepatology,Tianjin Institute of Hepatobiliary Disease,Tianjin Key Laboratory of Artificial Cells,Tianjin Third Central Hospital,Tianjin Medical University,83 Jintang Road,Tianjin 300170, China | 2010 | World Journal of Gastroenterology2010，16，5： | 20 |
| 15 | Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats显示文摘AIM: To investigate the effect of N-desulfated heparin onhepatic/renal ischemia and reperfusion injury in rats.METHODS: Using rat models of 60 minutes hepatic or renalischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animalswere randomly divided into following groups,the shamoperated controls,ischemic group receiving only normalsaline,and treated group receiving N-desulfated heparin ata dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in bepatic/renal tissueswith immunohistochemistry methodRESULTS: P-selectin expression, serum ALT, AST, BUN andCr levels were significantly increased during 60 minuteischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while theincrement was significantly inhibited,and hepatic/renalpathology observed by light microscopy was remarkablyimproved by treatment with the N-desulfated heparin.Furthermore,the heparin was found no effects on PT and KPTT.CONCLUSION: P-selectin might mediate neutrophilinfiltration and contribute to hepatic/renal ischemia andreperfusion. The N-desulfated heparin might preventhepatic/renal damage induced by ischemia and reperfusioninjury without significant anticoagulant activity. | Tong Zhou Wei Song Feng Wang Department of Nephrology,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Jin-Lian Chen Department of Gastroenterology,Shanghai Sixth People’s Hospital,Shanghai 200233,China Ming-Jun Zhang Animal Laboratory,Ruijin Hospital,Shanghai Second Medical University,Shanghai 200025,China Pei-Hua Ni,Department of Biochemistry,Shanghai Second Medical University,Shanghai 200025 Jian-Guo Geng Institute of Biochemistry and Cell Biology,Shanghai 200030,China | 2002 | World Journal of Gastroenterology2002，8，5： | 20 |
| 16 | The IAP family: endogenous caspase inhibitors with multiple biological activities显示文摘IAPs (inhibitors of apoptosis) are a family of proteins containing one or more characteristic BIR domains. These proteins have multiple biological activities that include binding and inhibiting caspases, regulating cell cycle progression, and modulating receptor-mediated signal transduction. Our recent studies found the IAP family members XIAP and c-IAP1 are ubiquitinated and degraded in proteasomes in response to apoptotic stimuli in T cells, and their degradation appears to be important for T cells to commit to death. In addition to three BIR domains, each of these IAPs also contains a RING finger domain.We found this region confers ubiquitin protease ligase (E3) activity to IAPs, and is responsible for the auto-ubiquitination and degradation of IAPs after an apoptotic stimulus. Given the factthat IAPs can bind a variety of proteins, such as caspases and TRAFs, it will be of interest to characterize potential substrates of the E3 activity of IAPs and the effects of ubiquitination byIAPs on signal transduction, cell cycle, and apoptosis. | YANG YI LI, XIAO MING LI(Laboratory of Immune Cell Biology, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA) | 2000 | Cell Research2000，10，3： | 19 |
| 17 | The cloning of 3'-truncated preS/S gene from HBV genomic DNA and its expression in transgenic mice显示文摘INTRODUCTIONHepatitis B virus (HBV) is regarded as one of themain etiologic factors involved in the developmentof human hepatocellular carcinoma (HCC).The open reading frame (orf)of X gene of HBVencoded a transactivating factor is the evidence thatstrongly supported the notion that the X gene ofHBV DNA integrated in HCC genomic DNA couldcontribute to the carcinogenesis of liver cells byactivation of some related cellular genes | Yi Ping Hu~1 Yu Cheng Yao~1 Jian Xiu Li~1 Xin Min Wang~1 Hong Li~2 Zhong Hua Wang~1 Zhang Heng Lei~3 1 Department of Cell Biology,Second Military Medical University,Shanghai 200433,China 2 Department of Biology,Department of Basic Medicine,West-China University of Medical Sciences,Chengdu 610041,China 3 Department of Biology,North Sichuan Medical College,Nanchong 637007,China | 2000 | World Journal of Gastroenterology2000，6，5： | 18 |
| 18 | Activation of JNK by TPA promotes apoptosis via PKC pathway in gastric cancer cells显示文摘AIM: JNK cascade plays an important role in cell proliferation, differentiation and apoptosis. However, the exact function of JNK cascade for apoptosis induction remains largely unknown. In this study, the role of JNK activation stimulated by TPA in the process of apoptosis induction and its signaling transduction pathway in gastric cancer cells were investigated and determined.METHODS: Expressions of mRNA and protein were detected by Northern blot and Western blot. Transcription activity was measured by transient transfection and CAT assay. Apoptotic cells were displayed through staining the nucleus with DAPI and were observed under fluorescence microscope. The apoptotic index was determined by counting 1000 cells randomly.RESULTS: JNK protein was stimulated rapidly by TPA, and reached its highest peak within 3 hr, then decreased in a time-dependent manner, but the expression level of JNK protein induced by TPA was always keeping higher than that in untreated cells. Similar pattern was seen in c-jun mRNA level induced by TPA. TPA significantly activated the transcriptional activity of activator protein-1 with a TPA-closedependent manner. Furthermore, activation of JNK was mediated through PKC pathway. Treatment of cells with PKC specific inhibitor, Wortmannin, led to repression of JNK even in the presence of TPA. More importantly, all these effects were associated with induction of apoptosis in gastric cancer cells. TPA inducted apoptosis obviously in gastric cancer cells. The apoptotic cells became smaller and rounded, and their nuclei became condensation and fragmentation with brightly stained chromatin. However, suppression of JNK by PKC specific inhibitor, Wortmannin, resulted in the decrease of apoptosis induced by TPA in a time-dependent manner, apoptotic index dramatically decreased from 32.56 % to 8.71%.CONCLUSION: TPA stimulates JNK cascade, including upregulation of JNK protein expression level and c-jun mRNA expression level, and activation of activator protein-1 transcriptional activity. Activation of JNK is mediated through PKC pathway, which has an association with induction of apoptosis by TPA. Thus, activation of JNK via PKC pathway may represent one of important mechanisms for TPA to induce apoptosis in gastric cancer cells. | Yan Chen Qiao Wu Si-Yang Song Wen-Jin Su,Key Laboratory of the Ministry of Education for Cell Biology and Tumor Cell Engineering,The School of Life Sciences,Xiamen University,Xiamen,Fujian Province,361005,China | 2002 | World Journal of Gastroenterology2002，8，6： | 18 |
| 19 | The sensitivity of digestive tract tumor cells to As_2O_3 is associated with the inherent cellular level of reactive oxygen species显示文摘AIM:To explore the correlation of the inherent cellular ROSlevel with the susceptibility of the digestive tract tumor cellsto apoptosis inducted by As_2O_3.METHODS:Two gastric cercinoma cell lines,SGC7901 andMKN45,and two esophageal carcinoma cell lines,EC/CUHKI(alternatively named EC1.71)and EC1867 with lowconcentration(2μmol·L^(-1))of As_2O_3 were cultured respectly,which confirmed the difference in apoptosis susceptibilitybetween SGC7901 and MKN45,and between EC/CUHK1 andEC1867.The cells were incubated withdihydrogenrhodamine123(DHR123),used as a ROScapture,in absence of As_2O_3.The fluorescent intensity ofrhodamine123,which was the product of cellular oxidationof DHR123,was detected by flow cytometry,and ROS wasmeasured.RESULTS:Apoptosis induced by a low concentration ofAs203 was more readily to occur in SGC7901(22.4%±2.4%)and EC/CUHK1(27.0%±2.9%)than in MKN45(2.1%±0.5%)and EC1867(0.8%±0.5%).In other words,SGC7901was more sensitive than MKN45 to As_2O_3,meanwhile EC/CUHK1 was more sensitive than EC1867 to As_2O_3.The levelof inherent cellular ROS in SGC7901(650±37)was higherthan that in MKN45(507±22)(P<0.01),and the level ofinherent cellular ROS in EC/CUHK1(462±17)was higherthan that in EC1867(187±12)(P<0.01).CONCLUSIONS.The cellular sensitivity to apoptosis inducedby As_2O_3 is associated with the difference in cellular ROSlevel.The inherent ROS level might determinate theapoptotic sensitivity of tumor cells to As_2O_3. | Fei Gao Jing Yi Gui-Ying Shi Hui Li Xue-Geng Shi Xue-Ming Tang Department of Cell Biology,Shanghai Second Medical University,Shanghai 200025,China | 2002 | World Journal of Gastroenterology2002，8，1： | 18 |
| 20 | Effect of retinoic acid on the changes of nuclear matrix-intermediate filament system in gastric carcinoma cells显示文摘AIM To explore the relationship between the configuration changes of the nuclear matrix-intermediate filament system in cancer cell induced by retinoic acid and the malignantphenotypic reversion of cancer cells.METHODS The human gastric adenocarcinomacell line MGc80-3 cells were induced with 10-6mol/ L retinoic acid and subouItured at cover slipstrip and gold grids. The cells were treated byselective extraction methOd and prepared for whole mount electron microscopy observation.The samples were examined respectively withscanning and transmission electron microscope.RESULTS The nuclear matrix filaments andintermediate filaments in MGC80-3 cells wererelatively few and scattered, not welldistributed and arranged irregularly. The nuclear lamina was ununiformly thick and compact,connected to the nuclear matrix filaments and intermediate filaments relaxedly. However, thetwo kinds of filaments were abundant and welldistributed, different in slender and thick form and interweaved into a regular network in the cells induced by 10-6 mol/ L RA. The nuclear matrix filaments and intermediate filaments were connected closely by the thin and compact fiber-Iike lamina, and interlaced into a regularnetwork throughout the whole cell region.CONCLUSION The NM-IF system in MGc80-3cells had undergone a restorational changesimilar to those of normaI cells after RAinducement. This alternation is an importantmorpholOgical and functional expression to themalignant phenotypic reversion of cancer cells. | Ll Qi-Fu(Laboratory of Cell Biology, XiamenUniversity, Xiamen 361O05, Fuian Province, China) | 1999 | World Journal of Gastroenterology1999，5，5： | 17 |
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