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| 1 | Sensitive markers used to identify compounds that trigger apoptosis in cultured hepatocytes显示文摘 | COMEZ-LECHON M J O CONNOR E CASTELL J V | 2002 | Toxicological Sciences2002，65，2： | 1 |
| 2 | Selective removal of fluoride ions by a two-way ion-exchange cyclic process显示文摘 | Castel C Schweizer M Simonot M O | 2000 | Chemical Engineering Science2000，55，17： | 1 |
| 3 | Operational Energy in the Life Cycle of Residential Dwellings: The Experience of Spain and Colombia 显示文摘 | Ortiz O Castells F Sonnemann G | 2010 | Applied Energy2010，，87： | 1 |
| 4 | Approach to the patient with unexplained chest pain显示文摘 | KATZ P O CASTELL D O | 2000 | Am J Gastroenterol2000，95，8： | 1 |
| 5 | Updated guidelines for the diagnosis and treatment of gastroesophageal reflux disease显示文摘 | De Vault R R Castell D O | 2005 | Am J Gastroenterol2005，100，1： | 1 |
| 6 | Sustainability in the construction industry:A review of recent developments based on LCA显示文摘 | O Ortiz F Castells G Sonnemann | | 0，，： | 1 |
| 7 | Changes in o-vernight fasting intragastric pH show poor correlationwith duodenogastric bile reflux in normal subjects显示文摘 | JUST R J LEITE L P CASTELL D O | 1996 | Am J Gastroenterol1996，91，15： | 1 |
| 8 | Updated guidelines for diagnosis and treatment of gastroesophageal reflux disease显示文摘 | Devauh K R Castell D O | 2005 | Am J Gastroenterol2005，100，1： | 1 |
| 9 | Nocturnal acid breakthrough -approach to management显示文摘 | TUTUIAN R CASTELL D O | 2004 | Med Gen Med2004，6，4： | 1 |
| 10 | Esomeprazole (40 mg) compared with lansoprazole (30 mg) in the treatment of erosive esophagitis显示文摘 | Castell D O Kahrilas P J Richter J E | 2002 | Am J Gastroenterol2002，97，： | 1 |
| 11 | A PPI is a PPI:lessons from prolonged intragastric pH monitoring显示文摘 | TUTUIAN R KATZ P O CASTELL D O | 2000 | Gastroenterology2000，118，： | 1 |
| 12 | Retrospective study of 145 supernumerary teeth显示文摘 | Femandez Montenegro P Valmaseda Castell o n E Berini Aytes L | 2006 | Med Oral Patol Oral Cir Bucal2006，11，4： | 1 |
| 13 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
| 14 | Development of a multiparametric cell-based protocol to screen and classify the hepatotoxicity potential of drugs 显示文摘 | Tolosa L Pinto S Donato Mr Ioz A Castell JV O'Connor JE | 2012 | Toxicol Sci2012，127，1： | 1 |
| 15 | Use of microencapsulated PCM in buildings and the effect of adding awnings显示文摘 | ARCE P CASTELL(O)N C CASTELL A | | 0，，07： | 1 |
| 16 | Selective removal of fluoride ions by a two-way ion-exchange cyclic process显示文摘 | CASTEL C SCHWEIZER M SIMONOT M O | 2000 | Chemical Engineering Science2000，55，17： | 1 |
| 17 | Acid secretion from an esophageal inlet patch demonstrated by ambulatory PH monitoring显示文摘 | Galan A R Katzka D A Castell D O | 1998 | Gastroenterology1998，115，： | 1 |
| 18 | Updated guidelines for thediagnosis and treatment of gastroesophageal refluxdisease显示文摘 | DEVAULT K R CASTELL D O | 2005 | Am J Gastroenterol2005，100，： | 1 |
| 19 | Pulmonary symp- toms associated with gastroesophageal reflux:use of am- bulatory pH monitoring to diagnose and to direct therapy 显示文摘 | Schnatz P F Castell J A Castell D O | 1996 | Am J gastroenterol1996，91，9： | 1 |
| 20 | Fetal microchimerism in patients with primary biliary cirrhosis显示文摘 | Selva O' Callaghan A Balada Prades E Castells FusteL | 2002 | Med Clin(Barc)2002，119，20： | 1 |
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