| 1 | 有机磷杀虫剂对M2受体磷酸化的影响显示文摘目的研究有机磷类杀虫剂对G蛋白结合受体激酶2介导的毒蕈碱乙酰胆碱m2受体磷酸化的影响和可能存在的其他作用途径。方法将纯化的毒蕈碱乙酰胆碱m2受体,G蛋白结合受体激酶-2,[γ-p32]ATP与对氧磷(PO)、氧毒死蜱(CPO)共同保温,聚丙烯酰胺凝胶电泳分离蛋白,放射性自显影检测m2受体磷酸化结果。结果氧毒死蜱完全抑制m2受体的磷酸化,其半数抑制浓度为(IC50)70μmol/L;毒死蜱也抑制m2受体的磷酸化,而对氧磷与对硫磷不抑制m2受体的磷酸化。结论不同的有机磷杀虫剂对m2受体的磷酸化有完全不同的作用;毒死蜱阻断m2受体的磷酸化,提示有机磷杀虫剂的毒性作用存在其他途径。 | 邹黎明 Carry Pope | 2005 | 中国公共卫生2005，21，11： | 4 |
| 2 | 有机磷类杀虫剂对毒蕈碱乙酰胆碱M_2受体磷酸化的影响显示文摘目的:研究有机磷类杀虫剂(OPs)对G蛋白结合受体激酶-2(GRK2)介导的毒蕈碱乙酰胆碱M2受体磷酸化的影响,进一步揭示有机磷类杀虫剂可能存在的其他作用途径。方法:利用亲和层析方法从猪脑中纯化毒蕈碱乙酰胆碱M2受体;将纯化的毒蕈碱乙酰胆碱M2受体,G蛋白结合受体激酶-2,[γ-p32]标记的ATP与不同浓度的对氧磷(PO)、氧毒死蜱(CPO)共同保温,聚丙烯酰胺凝胶电泳,凝胶片干燥后放射性自显影检测M2受体磷酸化结果。之后将干燥凝胶片中放射性标记的磷酸化M2受体蛋白带剪下,同位素液体闪烁计数器计数。结果:氧毒死蜱(CPO)完全抑制毒蕈碱乙酰胆碱M2受体的磷酸化,其IC50为70μM;毒死蜱(CPF)也抑制M2受体的磷酸化,而对氧磷(PO)与对硫磷(parath ion)不抑制M2受体的磷酸化。结论:不同的OPs对GRK2介导的毒蕈碱乙酰胆碱M2受体的磷酸化有完全不同的作用;毒死蜱阻断M2受体的磷酸化,提示OPs的毒性作用存在其他的可能作用途径。 | 邹黎明 李舒音 Carry Pope | 2005 | 沈阳医学院学报2005，7，3： | 0 |
| 3 | Effect of organophosphorus insecticides on phosphorylation of the M_2 muscarinic acetylcholine receptor显示文摘BACKGROUND: Organophosphorus insecticides may promote the accumulation of acetylcholine at synapses and the neuromuscular junction by inhibiting acetylcholinesterase activity to cause disturbance of neural signal conduction and induce a toxic reaction. Organophosphorus insecticides may act on M2 muscarinic acetylcholine receptors, whose combination with G proteins is regulated by phosphorylation of G protein-coupled receptor kinase 2. OBJECTIVE: To investigate the effects of organophosphorus insecticides on the phosphorylation of G protein-coupled receptor kinase 2-mediated M2 muscarinic acetylcholine receptors and to reveal other possible actions of organophosphorus insecticides. DESIGN, TIME AND SETTING: An observational study, which was performed in the Central Laboratory of Shenyang Medical College, and Department of Physiological Sciences, College of Veterinary Medicine, Oklahoma State University from June 2002 to December 2004. MATERIALS: Paraoxon, parathion, chlorpyrifos, and chlorpyrifos oxon were provided by Chem Service Company, USA, [γ-P32] ATP and [35S]GTPγS by New England Nuclear Life Science Products, and recombinant β2-adrenergic receptor membrane protein by Sigma Company, USA. METHODS: The M2 muscarinic acetylcholine receptor was extracted and purified from pig brain using affinity chromatography. Subsequently, the purified M2 muscarinic acetylcholine receptor, G protein-coupled receptor kinase 2, and [γ-P32] ATP were incubated with different concentrations of paraoxon and chlorpyrifos oxon together. The mixture then underwent polyacrylamide gel electrophoresis, and the gel film was dried and radioactively autographed to detect phosphorylation of the M2 muscarinic acetylcholine receptor. Finally, the radio-labeled phosphorylated M2 receptor protein band was excised for counting with an isotope liquid scintillation counter. MAIN OUTCOME MEASURES: Effects of chlorpyrifos oxon, paraoxon, chlorpyrifos, and parathion in different concentrations on the phosphorylation of the M2 muscarinic acetylcholine receptor; effects of chlorpyrifos oxon on the phosphorylation of theβ-adrenergic receptor. RESULTS: Chlorpyrifos oxon could completely inhibit the phosphorylation of the M2 muscarinic acetylcholine receptor, and its IC50 was 70 μmol/L. Chlorpyrifos could also inhibit the phosphorylation of the M2 muscarinic acetylcholine receptor. However, paraoxon and parathion could not inhibit the phosphorylation of the M2 muscarinic acetylcholine receptor. Chlorpyrifos oxon in different concentrations could also not inhibit the phosphorylation of the β2-adrenergic receptor catalyzed by G protein-coupled receptor kinase 2. CONCLUSION: Different kinds of organophosphorus insecticides have different effects on the phosphorylation of the G protein-coupled receptor kinase 2-mediated M2 muscarinic acetylcholine receptor. Organophosphorus insecticides possibly have different toxic effects. | Shuyin Li Liming Zou Carry Pope | 2008 | Neural Regeneration Research2008，3，4： | 0 |
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