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| 1 | MiRNA-206 inhibits hepatocellular carcinoma cell proliferation and migration but promotes apoptosis by modulating cMET expression显示文摘A close relationship between cancer progression and microRNAs(miRNAs)regulation has been demonstrated.Abnormal microRNA-206(miR-206)expression has been shown to be related to the development of malignancies.However,the role of miR-206 in hepatocellular carcinoma(HCC)remains unclear.Here,we evaluated the function of miR-206 in HCC.Results showed that miR-206 expression was decreased in 27 human HCC tissues compared with that of adjacent normal tissues.Conversely,cMET was up-regulated in human HCC cancer tissues,and cMET levels were shown to be negatively correlated with miR-206 expression.Abnormally increased miR-206 expression in three HCC cell lines(SMMC-7721,HepG2,and Huh7)attenuated cell viability,migration,and invasion.Increased apoptosis was also observed in these miR-206 expressing cells.Furthermore,we identified that miR-206 targets the 3′-UTR of the cMET gene for silencing,and restoration of cMET expression reversed the inhibitory effect of miR-206 on HCC.Tumor cells expressing miR-206 also showed delayed growth in the in vivo experiments compared with the controls.Altogether,our findings provide new insights into the molecular mechanisms of HCC oncogenesis. | Yuanxi Wang Qinwen Tai Jinhui Zhang Junsheng Kang Feng Gao Feng Zhong Liquan Cai Fa Fang Yi Gao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，3： | 7 |
| 2 | Role of αVβ3 integrin in embryo implantation in the mouse显示文摘Integrin, a heterodimeric adhesive molecule composed of a and (5 subunits, can regulate cell adhesion and trafficking. Recent data have documented that, at the 'implantation window' stage, aVp3 integrin participates in the maternal-fetal interaction and becomes a potential marker of uterine receptivity. Furthermore, it can affect invasiveness of embryo. This work made a further study about its action mechanism. Results of indirect immunofluorescence and laser scanning confocal microscopy showed that αVβ3 integrin was clearly expressed in the mouse blastocyst. Injection of αVβS integrin antiserum into a uterine horn of a pregnant mouse on day 3 markedly decreased the number of embryos implanted (P < 0.001). In a co-culture model, aVp3 integrin antisera at 1 : 100 and 1 : 200 dilutions significantly depressed the attachment and outgrowth reactions of blastocysts on monolayer of uterine epithelial cells. Analysis of correlation manifested that the inhibitory effect of αVβ3 integrin antiserum was | Liquan Cai Yujing Cao Enkui Duan | 2000 | Chinese Science Bulletin2000，45，22： | 6 |
| 3 | The expression and function of VEGF at embryo implantation 'window' in the mouse显示文摘Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that plays a critical role in angiogenesis. Recent reports indicated that VEGF was closely involved in embryo implantation and embryonic vasculogenesis. However, very little information is available about the detailed expression and function of VEGF at implantation 'window'. In this work, VEGFs were primarily present on uterine epithelial cell monolayer and blasto-cysts including the outgrew trophoblasts at implantation window. VEGF antibodies decreased the number of mice embryos implanted and the percentage of blastocysts with attachment and outgrowth in a co-culture model in a dose-dependant manner. These findings demonstrate that VEGF is one of the essential cytokines for embryo implantation in mouse. VEGF may act as a local mediator to regulate the maternal-fetal interaction, and facilitate blastocyst implantation. | Jing Zhang Li Wang Liquan Cai Yujing Cao Enkui Duan | 2001 | Chinese Science Bulletin2001，46，5： | 2 |
| 4 | Effects of Low Temperature Stress on Seedling Growth of Plateau Japonica Rice显示文摘[Objective] The study aimed to screen rational seedling raising date and seedling raising mode for plateau japonica rice,in order to reduce the effect of low temperature on seedlings. [Method]The seedlings of conventional japonica rice Hexi 22-2 and giant embryo No. 1 japonica rice were treated at day temperature 12℃ / night temperature 8℃ and day temperature 14 ℃ / night temperature 10 ℃,and the indicators including chlorophyll content,soluble sugar content,soluble protein content,survival rate,plant height,number of ≥ 1 cm roots,leaf wilting degree and aboveground dry matter weight were determined after treated for0,3 and 6 d,respectively. [Result]Chlorophyll content of seedlings decreased when treated by low temperature for a certain period; with the prolongation of treatment time,contents of soluble sugar and soluble protein in some low temperature treatments increased,while plant height,number of ≥ 1 cm roots and aboveground dry matter weight increased slowly,but the growth rate was significantly lower than that at room temperature; with the decrease of temperature and the prolongation of treatment time,leaf wilting degree increased. The comprehensive experimental results showed that with the increasing duration of low temperature,when two japonica rice varieties grew under the same low temperature,seedlings grew more and more slowly,probably because the lower the treatment temperature,the greater the damage on plants. Giant Embryo No. 1 had weaker cold tolerance than Hexi 22-2. [Conclusion]The study provides a theoretical basis for formulation of seedling raising technique of plateau japonica rice. | Dai Hongyan Hua Jinsong Zhang Rongping Cai Guangze Chen Liquan | 2015 | Plant Diseases and Pests2015，6，3： | 1 |
| 5 | Dynamic distribution of epidermal growth factor during mouse embryo peri-implantation显示文摘 | Cai Liquan Zhang Jian Duan Enkui | 2003 | Cytokine2003，23，6： | 1 |
| 6 | CRISPR-mediated genome editing and human diseases显示文摘CRISPR(Clustered Regularly Interspaced Short Palindromic Repeats)technology has emerged as a powerful technology for genome editing and is now widely used in basic biomedical research to explore gene function.More recently,this technology has been increasingly applied to the study or treatment of human diseases,including Barth syndrome effects on the heart,Duchenne muscular dystrophy,hemophilia,b-Thalassemia,and cystic fibrosis.CRISPR/Cas9(CRISPR-associated protein 9)genome editing has been used to correct diseasecausing DNA mutations ranging from a single base pair to large deletions in model systems ranging from cells in vitro to animals in vivo.In addition to genetic diseases,CRISPR/Cas9 gene editing has also been applied in immunology-focused applications such as the targeting of C-C chemokine receptor type 5,the programmed death 1 gene,or the creation of chimeric antigen receptors in T cells for purposes such as the treatment of the acquired immune deficiency syndrome(AIDS)or promoting anti-tumor immunotherapy.Furthermore,this technology has been applied to the genetic manipulation of domesticated animals with the goal of producing biologic medical materials,including molecules,cells or organs,on a large scale.Finally,CRISPR/Cas9 has been teamed with induced pluripotent stem(iPS)cells to perform multiple tissue engineering tasks including the creation of disease models or the preparation of donor-specific tissues for transplantation.This review will explore the ways in which the use of CRISPR/Cas9 is opening new doors to the treatment of human diseases. | Liquan Cai Alfred L.Fisher Haochu Huang Zijian Xie Marshal | 2016 | Genes & Diseases2016，3，4： | 1 |
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