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| 1 | Reproductive management through integration of PGD and MPS-based noninvasive prenatal screening/diagnosis for a family with GJB2-associated hearing impairment显示文摘A couple with a proband child of GJB2(encoding the gap junction protein connexin 26)-associated hearing impairment and a previous pregnancy miscarriage sought for a reproductive solution to bear a healthy child. Our study aimed to develop a customized preconception-to-neonate care trajectory to fulfill this clinical demand by integrating preimplantation genetic diagnosis(PGD), noninvasive prenatal testing(NIPT), and noninvasive prenatal diagnosis(NIPD) into the strategy. Auditory and genetic diagnosis of the proband child was carried out to identify the disease causative mutations. The couple then received in-vitro-fertilization treatment, and eight embryos were obtained for day 5 biopsy. PGD was performed by short-tandem-repeat linkage analysis and Sanger sequencing of GJB2 gene. Transfer of a GJB2 c.235del C heterozygous embryo resulted in a singleton pregnancy. At the 13 th week of gestation, genomic DNA(g DNA) from the trio family and cell-free DNA(cf DNA) from maternal plasma were obtained for assessment of fetal chromosomal aneuploidy and GJB2 mutations. NIPT and NIPD showed the absence of chromosomal aneuploidy and GJB2-associated disease in the fetus, which was later confirmed by invasive procedures and postnatal genetic/auditory diagnosis. This strategy successfully prevented the transmission of hearing impairment in the newborn, thus providing a valuable experience in reproductive management of similar cases and potentially other monogenic disorders. | XIONG WenPing WANG DaYong GAO Yuan GAO Ya WANG HongYang GUAN Jing LAN Lan YAN JunHao ZONG Liang YUAN Yuan DONG Wei HUANG SeXin WU KeLiang WANG YaoShen WANG ZhiLi PENG HongMei LU YanPing XIE LinYi ZHAO Cui WANG Li ZHANG QiuJing GAO Yun LI Na YANG Ju YIN ZiFang HAN Bing WANG Wei CHEN Zi-Jiang WANG QiuJu | 2015 | Science China(Life Sciences)2015，58，9： | 12 |
| 2 | Exogenous abscisic acid coordinating leaf senescence and transport of assimilates into wheat grains under drought stress by regulating hormones homeostasis显示文摘Drought at the grain filling stage of wheat will cause premature leaf senescence, thus leading to considerable loss of wheat yield. Therefore, this paper aims to establish a cultivation technology for strong drought resistance, delayed senescence, and yield improvement based on the analysis of hormones homeostasis obtained by applying chemical control substances. Experiments were conducted with two genotypes of wheat. Four water irrigation treatments were applied to impose the water deficit, including well-watered control treatment(WW), mild water deficit(MiWD), moderate water deficit(MoWD), and severe water deficit(SWD). Exogenous abscisic acid(ABA) was sprayed on the plants at the anthesis stage of the wheat. As a result, exogenous ABA reduced initial senescence rate(r0), total duration of chlorophyll(Chltotal), rapid senescence phase(Chlloss), and the accumulated temperature at an inflection point(M) but improved the persistence phase(Chlper) of flag leaves under all of the four treatments. However, exogenous ABA produced inconsistent effects on photoassimilate relocation and grain weight under different treatments. It produced positive regulatory effects on grain weight under WW, MiWD, and MoWD treatments. On the one hand, spraying ABA during the persistence phase of flag leaves reduced the ratios of zeatin to gibberellin(Z/GA3), spermine to spermidine(Spm/Spd), and salicylic acid to ABA(SA/ABA),which prolonged active photosynthesis by stimulating high level of proline(Pro) and increased the activities of antioxidant enzymes, such as superoxide dismutase(SOD), peroxidase(POD), catalase(CAT), and ascorbate peroxidase(APX). Therefore, drought tolerance was enhanced, and more photosynthetic assimilates were accumulated. On the other hand, the rapid senescence phase and the transport rate of assimilates into grains were accelerated, resulting in higher grain weight, yield, and water use efficiency(WUE). However, under SWD treatment, exogenous ABA improved the ratio of SA/ABA, leading to low Pro content and low antioxidant enzyme activity of flag leaves in the rapid loss phase. Meanwhile,drought resistance declined and the transport duration of assimilates into grains was shortened, thus making photosynthetic assimilates redundant. Therefore, exogenous ABA can lead to the reduction in grain weight, yield, and WUE of wheat under SWD treatment. | Yongli Luo Wenqian Li Cui Huang Junhao Yang Min Jin Jin Chen Dangwei Pang Yonglan Chang Yong Li Zhenlin Wang | 2021 | The Crop Journal2021，9，4： | 4 |
| 3 | Early changes to the extracellular space in the hippocampus under simulated microgravity conditions显示文摘The smooth transportation of substances through the brain extracellular space(ECS)is crucial to maintaining brain function;however,the way this occurs under simulated microgravity remains unclear.In this study,tracer-based magnetic resonance imaging(MRI)and DECS-mapping techniques were used to image the drainage of brain interstitial fluid(ISF)from the ECS of the hippocampus in a tail-suspended hindlimb-unloading rat model at day 3(HU-3)and 7(HU-7).The results indicated that drainage of the ISF was accelerated in the HU-3 group but slowed markedly in the HU-7 group.The tortuosity of the ECS decreased in the HU-3 group but increased in the HU-7 group,while the volume fraction of the ECS increased in both groups.The diffusion rate within the ECS increased in the HU-3 group and decreased in the HU-7 group.The alterations to ISF drainage and diffusion in the ECS were recoverable in the HU-3 group,but neither parameter was restored in the HU-7 group.Our findings suggest that early changes to the hippocampal ECS and ISF drainage under simulated microgravity can be detected by tracer-based MRI,providing a new perspective for studying microgravity-induced nano-scale structure abnormities and developing neuroprotective approaches involving the brain ECS. | Yajuan Gao Hongbin Han Jichen Du Qingyuan He Yanxing Jia Junhao Yan Hui Dai Bin Cui Jing Yang Xunbin Wei Liu Yang Rui Wang Ren Long Qiushi Ren Xing Yang Jiabin Lu | 2022 | Science China(Life Sciences)2022，65，3： | 2 |
| 4 | The effect of SARS-CoV-2 infection on human embryo early development:a multicenter prospective cohort study显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCo V-2)infection has swept the globe for 3 years(Zhou et al.,2020).With the nationwide relaxation of controls on the coronavirus disease 2019(COVID-19)epidemic since December 2022 in China,fertility and in vitro fertilization(IVF)centers are receiving increasing numbers of infected patients. | Xiaolei Chen Huangcong Shi Cheng Li Wanxia Zhong Linlin Cui Wenjun Zhang Ling Geng Kuona Hu Mei Fang Daimin Wei Junhao Yan Yun Sun Keliang Wu Han Zhao Zi-Jiang Chen | 2023 | Science China(Life Sciences)2023，66，7： | 1 |
| 5 | Intramolecular hydrogen bonds in a single macromolecule:Strength in high vacuum versus liquid environments显示文摘As a weak non-covalent interaction,hydrogen bond(H-bond)is highly susceptible to the environmental interference.However,the direct quantification of a single H-bond under an interference-free condition is still a challenge.Herein,the intramolecular H-bond in a model system,poly(N-isopropylacrylamide),is studied in high vacuum by single-molecule atomic force microscopy and steered molecular dynamics simulations,which allows the precise quantification of H-bond strength in an interference-free state.Control experiments show that the H-bond is significantly weakened in nonpolar solvent,even if the dielectric constant is very close to vacuum.If a polar solvent is used as the environment,the H-bond will be further weaker or even broken.These results imply that for experiments in any liquid environment,the H-bond strength(△G)will be only -50% or even less of that measured in vacuum.Further analysis shows that in liquid environments,AG decays in a quasi-linear way with the increase of the dielectric constant(ε).For H-bond studies in future,the result measured in vacuum can be set as the standard value,namely,the inherent strength.This approach will provide fundamental insights into the H-bond participated nano-structures and materials in different environments. | Wanhao Cai Duo Xu Fa Zhang Junhao Wei Song Lu Linmao Qian Zhongyuan Lu Shuxun Cui | 2022 | Nano Research2022，15，2： | 0 |
| 6 | In vivo imaging of the neuronal response to spinal cord injury:a narrative review显示文摘Deciphering the neuronal response to injury in the spinal cord is essential for exploring treatment strategies for spinal cord injury(SCI).However,this subject has been neglected in part because appropriate tools are lacking.Emerging in vivo imaging and labeling methods offer great potential for observing dynamic neural processes in the central nervous system in conditions of health and disease.This review first discusses in vivo imaging of the mouse spinal cord with a focus on the latest imaging techniques,and then analyzes the dynamic biological response of spinal cord sensory and motor neurons to SCI.We then summarize and compare the techniques behind these studies and clarify the advantages of in vivo imaging compared with traditional neuroscience examinations.Finally,we identify the challenges and possible solutions for spinal cord neuron imaging. | Junhao Deng Chang Sun Ying Zheng Jianpeng Gao Xiang Cui Yu Wang Licheng Zhang Peifu Tang | 2024 | Neural Regeneration Research2024，19，4： | 0 |
| 7 | VEGFR2 inhibition hampers breast cancer cell proliferation via enhanced mitochondrial biogenesis显示文摘Objective:Vascular endothelial growth factor(VEGF),apart from its predominant roles in angiogenesis,can enhance cancer cell proliferation,but its mechanisms remain elusive.The purpose of the present study was therefore to identify how VEGF regulates cancer cell proliferation.Methods:VEGF effects on cancer cell proliferation were investigated with the VEGF receptor 2 inhibitor,Ki8751,and the breast cancer cell lines,MCF-7 and MDA-MB-231,using flow cytometry,mass spectrometry,immunoblotting,and confocal microscopy.Data were analyzed using one-way analysis of variance followed by Tukey’s multiple comparison test.Results:VEGF blockade by Ki8751 significantly reduced cancer cell proliferation,and enhanced breast cancer cell apoptosis.Mass spectrometric analyses revealed that Ki8751 treatment significantly upregulated the expression of mitochondrial proteins,suggesting the involvement of mitochondrial biogenesis.Confocal microscopy and flow cytometric analyses showed that Ki8751 treatment robustly increased the mitochondrial masses of both cancer cells,induced endomitosis,and arrested cancer cells in the high aneuploid phase.VEGFR2 knockdown by sh RNAs showed similar effects to those of Ki8751,confirming the specificity of Ki8751 treatment.Enhanced mitochondrial biogenesis increased mitochondrial oxidative phosphorylation and stimulated reactive oxygen species(ROS)production,which induced cancer cell apoptosis.Furthermore,Ki8751 treatment downregulated the phosphorylation of Akt and PGC1α,and translocated PGC1αinto the nucleus.The PGC1αalterations increased mitochondrial transcription factor A(TFAM)expression and subsequently increased mitochondrial biogenesis.Conclusions:VEGF enhances cancer cell proliferation by decreasing Akt-PGC1α-TFAM signaling-mediated mitochondrial biogenesis,ROS production,and cell apoptosis.These findings suggested the anticancer potential of Ki8751 via increased mitochondrial biogenesis and ROS production. | Hao Ni Min Guo Xuepei Zhang Lei Jiang Shuai Tan Juan Yuan Huanhuan L Cui Yanan Min Junhao Zhang Susanne Schlisio Chunhong Ma Wangjun Liao Monica Nister Chunlin Chen Shuijie Li Nailin Li | 2021 | Cancer Biology & Medicine2021，18，1： | 0 |
| 8 | Understanding the Conformational Interconversions of a Polymer Chain in a Liquid Environment at the Single-molecule Level显示文摘The conformational interconversions of polymer chains have been of great interest as a basic scientific issue.Single-molecule force spectroscopy(SMFS)is a powerful tool for molecular manipulation,which enables experimental studies on the single-chain behaviors of polymers.The SMFS results show that an individual polymer chain in a liquid environment may have similar properties to an ideal chain,which contradicts the traditional theoretical view.Herein,by taking into account the collisions of solvent molecules,the conformational interconversions of a single polymer chain in a liquid environment have been analyzed.The conformational interconversion frequency of a carbon-carbon bond of an alkane chain can be estimated by establishing the relationship between the internal rotation barriers of small molecules(monomers)and the corresponding macromolecules.Since the time scale of conformational interconversions of the polymer backbone is much shorter than that of SMFS experiments,most polymers with C-C backbones behave as ideal chains in liquid environments. | SHAO Yue WEI Junhao BAO Yu CAI Wanhao QIAN Lu CUI Shuxun | 2023 | Chemical Research in Chinese Universities2023，39，5： | 0 |
| 9 | Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in Pyropia yezoensis (Bangiales,Rhodophyta)显示文摘The genus Pyropia contains several important cultivated species.Genetic research in nori species has mainly focused on the cell nucleus,with few studies on organelles(chloroplast and mitochondria).Due to the high copy numbers of organelles in cells,which influence the development and traits of algae,it is necessary to study their genetic mechanism.In this study,the marine red alga Pyropia yezoensis,an important economic macroalga,was selected as the study object.To investigate organelle(chloroplast and mitochondria)inheritance in P.yezoensis,the wild type RZ(maternal strain)was crossed with the red mutant HT(paternal strain)and 30 color-sectors from 11 F1 gametophytic blades were examined.The complete chloroplast and mitochondrial genomes of the red mutant(HT)were assembled for the first time.One reliable and stable single nucleotide polymorphism(SNP)loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA,respectively,in subsequent experiments.PCR amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent,confirming that both chloroplast and mitochondrial genomes were inherited maternally in P.yezoensis.The inheritance pattern of organelles in P.yezoensis can be used to guide the hybridization and breeding of nori.Additionally,the organelle SNP markers developed in this study can be applied in subsequent genetic research. | Lu WANG Junhao WANG Yunke ZHU Zhengcai CUI Fanna KONG Xianghai TANG Yunxiang MAO | 2021 | Journal of Oceanology and Limnology2021，39，4： | 0 |
| 10 | Ore-controlling Regularitiesof Structureinthe Lehong Lead-zinc Deposit, Northeastern Yunnan显示文摘1 Introduction The Lehonglead-zincdeposit is a large-sized Pb-Zn depositnewly found in recent years in the Sichuan-Yunnan-Guizhou Lead-zinc Poly-metallic Mineralization Area,which occurrenceis | CUI Junhao HAN Runsheng ZHAO Dong ZHANG Xiaopei | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
| 11 | Tectonic Geochemical Characteristics and Ore Prediction in Hongdoushan Copper Deposit, Yunxian, Yunnan显示文摘South Lancangjiang metallogenic condition is superior,the volcanic arc metallogenic belt has been praised by geologists,the north district of Guanfang,Wenyu copper prospecting research has made important progress in recent years,the paper study in the Hongdoushan mountain copper deposit(the newly discovered deposits) | GUO Zehua WANG Lei HAN Runsheng WANG Jiasheng BAI Longan QIU Wenlong LIU Fei CUI Junhao TAN Wei | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
| 12 | Quantitative Analysis of Mineralization-Alteration Index about the Huize Lead-zinc district in Northeast Yunnan, China显示文摘1 Introduction The super-large Huize lead-zinc ore district is one of the most representative lead-zinc deposits in Northeastern Yunnan poly-metallic deposit concentration district.So far,studies(Liu and Lin,1999;Han et al.,2007)on the type of deposits like the Huize lead-zinc deposit,focused on deposit genesis,the sources of metallogenic materials, | ZHANG Xiaopei HAN Runsheng ZHAO Dong QIU Wenlong HE Jiaojiao CUI Junhao | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
| 13 | Metallogenic Processes:Evidencesfrom Zoning Patterns of Mineralization and alteration and fluid inclusion geochemistryin the Lehong Zn-Pb Deposit in Northeastern Yunnan, China显示文摘1 Introduction The large clusters of Zn-Pb deposits in northeastern Yunnan,located in the southwestern margin of the Yangtze Block,are an important part of the Sichuan-YunnanGuizhou Pb-Zn Poly-metallic Metallogenic Triangle | ZHAO dong HAN Runshen REN Tao WANG Jiasheng ZHANG Xiao-pei WU Yongtao CUI Junhao | 2017 | Acta Geologica Sinica(English Edition)2017，91，S1： | 0 |
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