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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Sequential therapies for proliferative lupus nephritis显示文摘 | Contreras G Pardo V Leclercq B | | 0，，： | 2 |
| 3 | Geneticmodification of liver grafts with an adenoviral vector encoding the Bcl-2 geneimproves organ preservation显示文摘 | Bilbao G Contreras JL Gomez Navarro J Eckhoff DE Mikheeva G Krasnykh V Hynes T Thomas FT Thomas JM Curiel DT | | 0，，： | 2 |
| 4 | Sequential therapies for proliferative lupus nephritis显示文摘 | CONTRERAS G PARDO V LECLEREQ B | 2004 | New Engl J Med2004，，350： | 1 |
| 5 | An epidemiologic study of early renal replacement therapy after orthotopic liver transplantation显示文摘 | Contreras G Garces G Quartin AA | 2002 | J Am Soc Nephrol2002，13，1： | 1 |
| 6 | Influence of race/ ethnicity onresponse to lupus nephritis treatment:the ALMS study显示文摘 | Isenberg D Appel GB Contreras G etal | 2010 | Rheumatology2010，49，1： | 1 |
| 7 | Sequential therapies for proliferative lupus nephritis显示文摘 | Contreras G Pardo V Leclercq B | 2004 | N Engl J Med2004，350，10： | 1 |
| 8 | Mycophenolate mofetil versus cyclophosphamide for induction treatment of lupus nephritis 显示文摘 | Appel GB Contreras G Dooley MA | 2009 | J Am Soc Nephrol2009，20，5： | 1 |
| 9 | Factors associated with poor outcomes in patients with lupus nephritis显示文摘 | Pardo V Cely C | 2005 | Lupus2005，14，： | 1 |
| 10 | TNF-αand IL-10 promoter polymorphisms,HPV infection,and cervical cancer risk显示文摘 | Barbisan G Perez LO Contreras A | 2012 | Tumour Biol2012，33，5： | 1 |
| 11 | Microstmcture and properties of hercynite- magnesia-calcium zirconate refractory mixtures 显示文摘 | Contreras J E Castillo G A | 2005 | Materials Characterization2005，54，11： | 1 |
| 12 | Sequential therapies for proliferative lupus nephritis显示文摘 | CONTRERAS G PARDO V LECLERCQ B | 2004 | N Engl J Med2004，350，10： | 1 |
| 13 | Factors associated with poor outcomes in patients with lupus nephritis显示文摘 | Pardo V Cely C | 2005 | Lupus2005，14，11： | 1 |
| 14 | Treatment options for severe lupus nephritis 显示文摘 | LENZ O CONTRERAS G | 2004 | Arch Immunol Ther Exp (Warsz)2004，52，5： | 1 |
| 15 | Microstructure and properties of hercynite-magnesia-calcium zirconate refractory mixtures 显示文摘 | Contreras J E Castillo G A | 2005 | Materials Characterization2005，54，11： | 1 |
| 16 | Sequential therapies for proliferative lupus nephritis显示文摘 | Contreras G Pardo V Leclercq B | 2004 | N Engl J Med2004，350，10： | 1 |
| 17 | Sequential ther- apies for proliferative lupus nephritis显示文摘 | Contreras G Pardo V Leclercq B | 2004 | N Engl J Med2004，350，： | 1 |
| 18 | Sequential therapies for proliferative lupus nephritis 显示文摘 | Contreras G Pardo V Leelercq B | 2004 | N Engl J Med2004，350，10： | 1 |
| 19 | Management of distal anterior cerebral artery aneu- rysms: part I: anatomic variations and clinical presentation显示文摘 | Abdo M Guinto G Contreras R | 2011 | Contemp Neurosurg2011，33，： | 1 |
| 20 | Pigment epithelium de- rived factor overexpression inhibits orthotopic osteosarcoma growth, angiogenesis and metastasis显示文摘 | Ek E T Dass C R Contreras K G | 2007 | Cancer Gene Ther2007，14，7： | 1 |
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