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| 1 | Clinical observation of salvianolic acid B in treatment of liver fibrosis in chronic hepatitis B显示文摘AIM: To evaluate the clinical efficacy of salvianolic acidB (SA-B) on liver fibrosis in chronic hepatitis B.METHODS: Sixty patients with definite diagnosis of liverfibrosis with hepatitis B were included in the trial.Interferon-γ (IFN-γ) was used as control drug. Thepatients took orally SA-B tablets or received muscularinjection of IFN-γ in the double blind randomized test,The complete course lasted 6 months. The histologicalchanges of liver biopsy specimen before and after thetreatment were the main evidence in evaluation, incombination with the results of contents of serum HA,LN, Ⅳ-C, P-Ⅲ-P, liver ultrasound imaging, andsymptoms and signs.RESULTS: Reverse rate of fibrotic stage was 36.67 % inSA-B group and 30.0 % in IFN-γgroup. Inflammatoryalleviating rate was 40.0 % in SA-B group and 36.67 %in IFN-γ group. The average content of HA and Ⅳ-Cwas significantly lower than that before treatment. Theabnormal rate also decreased remarkably. Overallanalysis of 4 serological fibrotic markers showedsignificant improvement in SA-B group as comparedwith the IFN-γgroup. Score of liver ultrasound imagingwas lower in SA-B group than in IFN-γgroup (HA 36.7 %vs80 %,Ⅳ-C 3.3 % vs23.2 %). Before the treatment,ALT AST activity and total bilirubin content of patientswho had regression of fibrosis after oral administrationof SA-B, were significantly lower than those of patientswho had aggravation of fibrosis after oraladministration of SA-B. IFN-γ showed certain sideeffects (fever and transient decrease of leukocytes,occurrence rates were 50 % and 3.23 %), but SA-Bshowed no side effects.CONCLUSION: SA-B could effectively reverse liverfibrosis in chronic hepatitis B. SA-B was better than IFN-γ in reduction of serum HA content, overall decrease of4 serum fibrotic markers, and decrease of ultrasoundimaging score. Liver fibrosis in chronic hepatitis B withslight liver injury was more suitable to SA-B in anti-fibrotic treatment. SA-B showed no obvious side effects. | Ping Liu Yi-Yang Hu Cheng Liu Hui-Ming Xue Zhi-Qiang Xu Lie-Ming Xu Cheng-Hai Liu Hong-Tu Gu Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China Da-Yuan Zhu Shanghai Institute of Metaria Medica,Chinese Academy of Sciences,Shanghai 200031,China Zhi-Qing Zhang the 4~(th) Hualyin City Hospital,Hualan City,223000,JiangSu Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 85 |
| 2 | 基本地震属性在沉积环境解释中的应用研究显示文摘地震属性在碳氢检测、储层参数预测和层序地层解释中已有广泛的应用,但将地震属性用于沉积环境解释却不多见。本文的目的旨在推进这方面的研究工作。在种类繁多的地震属性中,瞬时振幅、瞬时频率、瞬时相位和相干数据体、波形聚类可以定义为基本地震属性,其他地震属性均与这5种基本地震属性有直接或间接关系。这5种基本属性均能反映某种沉积现象:瞬时振幅可以反映地层的波阻抗(速度及密度)和孔隙流体的差异;瞬时相位可以刻画岩性变化的边界和一些异常体的边界;相干数据体可以反映地质体的空间变化;波形聚类可以反映地质体的空间相似程度。为了提高采用基本地震属性解释沉积环境的可信度,用宽方位角观测是十分有益的。采用相对保持振幅与高分辨率处理是必要的条件,在解释中如何合理选择参考标准层进行井信息标定也是成功的重要保证。选择参考标准层的条件是:①该层在研究区具有相同的沉积环境;②该层和目的层间必须在一个沉积旋回内,或处在一个地震波周期内。研究表明,采用上述方法提取的地震属性可以获得地震分辨率小于1/4波长的沉积环境信息,就是说,此法可以用于沉积微相的解释和预测,为寻找有利储集相带提供重要信息。 | Ling Yun, BGP, CNPC, Zhuozhou City, Hebei Province, 072751,China | 2003 | 石油地球物理勘探2003，38，6： | 164 |
| 3 | 湖南洪江市高庙新石器时代遗址显示文摘该遗址是一处典型的新石器时代贝匠遗址。下部的'高庙文化'遗存中发现大型祭祀场所及墓葬、房址等遗迹,出土大量精美白陶器及装饰复杂图案的陶器。高庙上层遗存则发现了较多房址及墓葬,出土大量陶器和石、骨质工具等。 | Neolithic Gaomiao Site in Hongjiang City,Hunan | 2006 | 考古2006，，7： | 56 |
| 4 | Expression,deleton and mnutation of ρ16 gene in human gastric cancer显示文摘AIM To investigate the relationship between the expression of p16 gene and the gastric carcinogenesis,depth of invasion and lymph node metastases, and to evaluate the deletion and mutation of exon 2 in p16 gene in gastric carcinoma.METHODS The expression of P16 protein was examined by streptavidin-peroxidase conjugated method (S-P); the deletion and mutation of p16 gene were respectively examined by polymerase chain reaction (PCR) and polymerase chain reaction single-strand conformation polymorphism analysis (PCR-SSCP) in gastric carcinoma.RESULTS Expression of P16 protein was detected in 96.25% (77/80) of the normal gastric mucosa, in 92.00% (45/50) of the dysplastic gastric mucosa and in 47.54% (58/122) of the gastric carcinoma. The positive rate of P16 protein expression in gastric carcinoma was significantly lower than that in normal gastric mucosa and dysplastic gastric mucosa (P<0.05). The positive rate of P16 protein expression in mucoid carcinoma 10.00% (1/ 10) was significantly lower than that in poorly differentiated carcinoma 51.22% ( 21/ 41 ),undifferentiated carcinoma 57.69% (15/26) and signet ring cell carcinoma 62.50% (10/ 16) (P<0.05). The positive rate of p16 protein in 30 cases paired primary and lymph node metastatic gastric carcinoma: There was 46.67% (14/30) in primary gastric carcinoma, 16.67% (5/30) in lymph node metastatic gastric carcinoma. The positive rate of lymph node metastatic carcinoma was significantly lower than that of primary carcinoma (P<0.05). There was of p16 gene mutation in exon 2, but 5 cases displayed deletion of p16 gene in exon 2 in the 25 primary gastric carcinomas.CONCLUSIONS The expression loss of P16 protein related to the gastric carcinogenesis, gastric carcinoma histopathological subtypes and lymph metastasis. The mutation of p16 gene in exon 2 may not be involved in gastric carcinogenesis. But the deletion of p16 gene in exon 2 may be involved in gastric carcinogenesis. | Xiu-Sheng He Qi Su Zhu-Chu Chen Xiu-Tao He Zhi-Feng Long Hui Ling Liang-Run Zhang Oncology Institute,Nanhua University,Hengyang 421001,Hunan Province,ChinaOncology Institute,Center South University,Changsha 410078,Hunan Province,China Department of Gastroenterology,First People’s Hospital of Changde City,Changde 415003,Hunan Province,China | 2001 | World Journal of Gastroenterology2001，7，4： | 40 |
| 5 | 沈阳郑家洼子的两座青铜时代墓葬显示文摘郑家洼子位于沈阳西南,东距沈阳站约5公里,南距浑河3公里,地势低洼,多沼泽,故名'洼子'。1958年春,曾在洼子北边发现一组青铜器,包括短剑等共二十七件。 | The Former Palace Museum of Shenyang and CPAM, City of Shenyang | 1975 | 考古学报1975，，1： | 40 |
| 6 | 西安相家巷遗址秦封泥的发掘显示文摘西安相家巷遗址(简称“CH相”)位于西安市未央区六村堡乡相家巷村南的东西向公路以南500米处,其东邻一条南北向的干渠,南靠一条东西向的土路。该遗址地处汉长安城桂宫遗址东北角外侧(图一;图二)。1996年,相家巷村村民在此地挖积肥坑时,发现了大量封泥。 | Han Chang’an City Archaeological Team,IA,CASS | 2001 | 考古学报2001，，4： | 36 |
| 7 | Helicobacter pylori infection generated gastric cancer through p53-Rb tumor-suppressor system mutation and telomerase reactivation显示文摘AIM: To investigate the relationship between Helicobacterpylori (H. pylori) infection and the expressions of the p53,Rb, c-myc, bcl-2 and hTERT mRNA in a series of diseasesfrom chronic gastritis (CG), intestinal metaplasia type Ⅰ or Ⅱ(IMⅠ-Ⅱ), intestinal metaplasia type Ⅲ (IMⅢ), mild or modestdysplasia (DysⅠ-Ⅱ), severe dysplasia (DysⅢ) to gastric cancer(GC) and to elucidate the mechanism of gastriccarcinogenesis relating to H.pyloriinfection.METHODS: 272 cases between 1998 and 2001 wereavailable for the study including 42 cases of CG, 46 cases ofIMⅠ-Ⅱ, 25 cases of IMⅢ, 48 cases of DysⅠ-Ⅱ, 27 cases ofDysⅢ, 84 cases of GC.-H. pyloriinfection and the expressionsof p53, Rb, c-myc, bcl-2 were detected by means ofstreptavidin-peroxidase (SP) immunohistochemical method.HTERT mRNA was detected byin situ hybridization(ISH).RESULTS: The expressions of p53, Rb, c-myc, hTERT mRNAand bcl-2 were higher in the GC than in CG, IN, Dys. Theexpression of c-myc was higher in IMⅢ with-H.pyloriinfection(10/16) than that without infection (1/9) and the positive ratein DysⅠ-Ⅱ and DysⅢ with-H.pyloriinfection was 18/30 and 13/17, respectively, higher than that without infection (4/18 and3/10, respectively). In our experiment mutated p53 had noassociation with H.pyloriinfection, theexpression of Rb wasassociated with-H. pyloriinfection in GC, but the p53-Rb tumor-suppressor system abnormal in DysⅠ-Ⅱ cases, DysⅢⅡ and GCcases with H. pyloriinfection was 21/30, 15/17 and 48/48respecively, higher than non-infection groups (4/18, 3/10, 28/36). Furthermore the level of hTERT mRNA in GC with H. pyloriinfection (47/48) was higher than that without infection (30/36), however the relationship between bcl-2 and H. pyloriwasonly in IMⅢ. C-myc had a close association with hTERT mRNAin DysⅢ and GC (P=0.0 253,0.0 305 respectively).CONCLUSION: In the gastric carcinogenesis, H. pylorimightcause the severe imbalance of proliferation and apoptosisin the precancerous lesions (IMⅢ and GysⅢ) first, leadingto p53-Rb tumor-suppressor system mutation and telomerasereactivation, and finally causes gastric cancer. | Jing Lan Yong-Yan Xiong Yi-Xian Lin Bi-Cheng Wang Ling-Ling Gong Hui-Sen Xu Guang-Song Guo Department of Pathology,Zhongnan Hospital,Wuhan University,Wuhan city 430071,Hubei Province,China | 2003 | World Journal of Gastroenterology2003，9，1： | 39 |
| 8 | Clinicopathological features of gastric glomus tumor显示文摘AIM:To study the clinicopathological features of gastric glomus tumor and review the related Chinese literature published in 1990-2010.METHODS:A case of gastric glomus tumor was re-ported.Clinicopathological findings in 56 cases of gastric glomus tumor were analyzed.RESULTS:Gastric glomus tumor was far more common in women than in men with a female to male ratio of 1.6:1.The median age of the patients was 45 years(range 28-79 years).The patients often complained of epigastric pain and bloody stool.The tumor was located in antrum of the stomach.The greatest diameter of the tumor was 0.8-11cm.Histologically,the tumor was comprised of nests of glomus cells surrounding the capillaries.Glomus cells were small,uniform and round.Vimentin,smooth muscle actin and actin were expressed in the tumor.Other markers,including S-100 protein,CD34,CD117,desmin,CD56,synaptophysin,chromo-granin A,neuron specific enolase and cytokeratin were all negative.CONCLUSION:Gastric glomus tumor is a rare benign mesenchymal neoplasm.Its diagnosis depends on pathologic examination.Differential diagnosis includes gastrointestinal stromal tumor,paraganglioma and carcinoid tumor. | Hui-Qiong Fang,Jing Yang,Fen-Fen Zhang,An-Jia Han,Department of Pathology,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,Guangdong Province,China Hui-Qiong Fang,Department of Pathology,Foshan Hospital of Traditional Chinese Medicine,Foshan 528000,Guangdong Province,China Jing Yang,Department of Pathology,People’s Hospital of Zhu-hai City,Zhuhai 519000,Guangdong Province,China Yi Cui,Department of Gastroenterology,First Affiliated Hospital of Sun Yat-Sen University,Guangzhou 510080,Guangdong Province,China | 2010 | World Journal of Gastroenterology2010，16，36： | 28 |
| 9 | 城市导视系统设计显示文摘城市导视系统设计是指一个完整的城市指示系统,包括行政交通导视系统、商业导视系统、文化旅游导视系统等。城市导视系统系统是城市形象和城市功能的重要组成部分,其合理设计和运用不仅可以使城市生活更加便利,而且也是使城市具有魅力的形象塑造。本文对国内国际上的优秀案例进行分析,并就城市导视系统设计的功能、审美特征以及实施方法进行了探讨。 | City design Institute,Central Academy of Fine Art,Beijing 100009) | 2004 | 城市发展研究2004，11，1： | 24 |
| 10 | A statistical model for porous structure of rocks显示文摘The geometric features and the distribution properties of pores in rocks were in- vestigated by means of CT scanning tests of sandstones. The centroidal coordi- nates of pores, the statistic characterristics of pore distance, quantity, size and their probability density functions were formulated in this paper. The Monte Carlo method and the random number generating algorithm were employed to generate two series of random numbers with the desired statistic characteristics and prob- ability density functions upon which the random distribution of pore position, dis- tance and quantity were determined. A three-dimensional porous structural model of sandstone was constructed based on the FLAC3D program and the information of the pore position and distribution that the series of random numbers defined. On the basis of modelling, the Brazil split tests of rock discs were carried out to ex- amine the stress distribution, the pattern of element failure and the inosculation of failed elements. The simulation indicated that the proposed model was consistent with the realistic porous structure of rock in terms of their statistic properties of pores and geometric similarity. The built-up model disclosed the influence of pores on the stress distribution, failure mode of material elements and the inosculation of failed elements. | JU Yang1,2, YANG YongMing1, SONG ZhenDuo3 & XU WenJing4 1 State Key Laboratory of Coal Resources and Safe Mining, China University of Mining & Technology, Beijing 100083, China 2 Department of Mechanical and Manufacturing Engineering, The University of Calgary, 2500 University Drive NW Calgary, AB T2N 1N4, Canada 3 China National Coal Mining Equipment Co. Ltd, Beijing 100011, China 4 Department of Materials and Metallurgy, The University of Utah, Salt Lake City, Utah 84112, USA | 2008 | Science China(Technological Sciences)2008，51，11： | 21 |
| 11 | Effects of aminoguanidine on nitric oxide production induced by inflammatory cytokines and endotoxin in cultured rat hepatocytes显示文摘AIM To study the effects of aminoguanidine(AG) and two L-arginine analogues Nω-nitro-L-arginine methyl ester (L-NAME) and Nω-nitro-L-arginine (L-NNA) on nitric oxide (NO) productioninduced by cytokines (TNF-α, IL-11β, and IFN-γ)and bacterial lipopolysaccharide (LPS) mixture(CM) in the cultured rat hepatocytes, andexamine their mechanisms action.METHODS Rat hepatocytes were incubatedwith AG, L-NAME, L-NNA, Actinomycin D (ActD)and dexamethasene in a medium containing CM(LPS plus TNF-α, IL-1β, and IFN-γ) for 24 h. NOproduction in the cultured supernatant wasmeasured with the Griese reaction. IntracellularcGMP level was detected with radioimmunoasey.RESULTS NO production was markedlyblocked by AG and L-NAME in a dose-dependentmanner under inflammatory stimuli conditiontriggered by CM in vitro. The rate of themaximum inhibitory effects of L-NAME (38.9%)was less potent than that obtained with AG(53.7%, P<0.05). There was no significantdifference between the inhibitory effects of AGand two L-arginine analogues on intracellularcGMP accumulation in rat cultured hepatocytes.Non-specific NOS expression inhibitordexamethasone ( DEX ) and iNOS mRNAtranscriptional inhibitor ActD also significantlyinhibited CM-induced NO production. AG(0.1mmol.L-1) and ActD (0.2ng@Lt) wereequipotent in decreasing NO production inducedby inflammatory stimuli in vitro, and botheffects were more potent than that induced bynon-selectivity NOS activity inhibitor L-NAME(0. 1 mmol@ L- 1) under similar stimuli conditions(P | Guo Liang Zhang Ye Hong Wang Hui Ling Teng Zhi Bin Lin Department of Pharmacology,School of Basic Medical Sciences,Beijing University,Beijiog 100083,ChinaDr.Guo Liang Zhang graduated from Xinxiang Medical College in 1982,got Ph.D.at Nagoya City University Medical School,Japan in 1994,finished postdoctoral research at Beijing Medical Univcrsity in 1996,now an associate professor of pharmacology,specialized in hepatic pharmacology,having 15 papers published. | 2001 | World Journal of Gastroenterology2001，7，3： | 20 |
| 12 | 四川荥经南罗坝村战国墓显示文摘1988年1月,荥经县塔子山茶场在荥经县附城乡南罗坝村的基建工程中,发现古文化遗物。我馆闻讯后当即派人前往调查,该地为战国时代墓地,在约二百平方米的范围内,我们发掘和清理墓葬十一座(88YLMl—11)。墓地位于经河与烟竹河交汇的二级台地上,北距经河800米,隔河与县城相望。城北即是荥河冲积台地上的同心村巴蜀墓地,两墓地相距仅1公里。墓地东500米为烟竹河,南是打罗坪山,山顶平坦,多为稻田。墓地附近为褐色土,耕土厚27—30厘米,以下即为砂砾层(图一)。 | Yingjing Museum of Yandao City Site | 1994 | 考古学报1994，，3： | 20 |
| 13 | 扬州宋大城西门发掘报告显示文摘The excavation of the western gate of the Great City, Song period Yangzhou, discovered the stratigraphical superimposition of the western gates respectively belonging to the Five Dynasties period Zhou Dynasty Lesser City, the Northern Song period District City, the Southern Song period Great City and the Ming and Qing Yangzhou City,brought to light the formal evolution of this city-gate, and revealed the historical mystery that the Zhou Dynasty Lesser City was also the Song period Great City. At the end of the Northern Song period, the Song and Jin dynasties were confronting each other, and Yangzhou became an anti-Jin forward position. To meet military needs, a barbican was added outside the western city-gate. The gate itself was built of bricks and must have been vaulted judging from the structure of the two side gateways. The great importance of this discovery lies in the fact that the construction of this city-gate was more than 130 years earlier than that of the vaulted city-gate of Heyimen in the Yuan Dadu. The typological features of the western gate cum barbican of the Song period Great City distinctly reflect the defensive function of the three cities of Song period Yangzhou, and provide valuable material for studying ancient urban architecture, military defence and the character of Song period Yangzhou City. At present the ruined western gate of the Song period Great City has constructed into a museum, constituting a part of the treatured heritage in the historic city of Yangzhou. | (Yangzhou City Archaeological Team of the Institute of Archaeology,CASS, the Nanjing Museum and the Yangzhou Municipal Bureau of Culture) | 1999 | 考古学报1999，，4： | 15 |
| 14 | Characteristics of the microbial communities in the integrated vertical-flow constructed wetlands显示文摘Microorganisms play an important role in removing pollutants from constructed wetlands.We investigated the microbial characteristics in a novel integrated vertical-flow constructed wetland(IVCW) ,which has been in operation in Wuhan,China since 1998.We used phospholipid fatty acid(PLFA) and amoA gene to analyze the structure and diversity of the microbial community within the IVCW.PLFA results suggested that the amount of bacterial PLFA was significantly higher than that of fungal PLFA,but the total microbial biomass represented by PLFA index was low in the system.Microbial spatial distribution showed significantly higher bacterial(both G+and G-) and fungal biomass in the surface than in the subsurface layers.The ratios of monounsaturated to branched PLFA demonstrated that an anaerobic layer sandwiched by two aerobic layers existed in the IVCW,consistent with the redox potential results.Analysis of the amoA revealed the presence of Nitrosomonas-like sequences in the surface substrate of the downflow chamber and apparent diversities of ammonia-oxidizing bacteria in the system.These results suggest that microorganisms,despite their relatively low biomass,have inhabited the IVCW,and the results will offer some valuable information on microbe to system designers and managers. | ZHOU Qiaohong,HE Feng,Zhang Liping,WANG Yanfen,WU Zhenbin State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China Department of Biology and Chemistry,City University of Hong Kong | 2009 | Journal of Environmental Sciences2009，21，9： | 14 |
| 15 | Characterizing and estimating rice brown spot disease severity using stepwise regression,principal component regression and partial least-square regression显示文摘Detecting plant health conditions plays a key role in farm pest management and crop protection. In this study, measurement of hyperspectral leaf reflectance in rice crop (Oryzasativa L.) was conducted on groups of healthy and infected leaves by the fungus Bipolaris oryzae (Helminthosporium oryzae Breda. de Hann) through the wavelength range from 350 to 2 500 nm. The percentage of leaf surface lesions was estimated and defined as the disease severity. Statistical methods like multiple stepwise regression, principal component analysis and partial least-square regression were utilized to calculate and estimate the disease severity of rice brown spot at the leaf level. Our results revealed that multiple stepwise linear regressions could efficiently estimate disease severity with three wavebands in seven steps. The root mean square errors (RMSEs) for training (n=210) and testing (n=53) dataset were 6.5% and 5.8%, respectively. Principal component analysis showed that the first principal component could explain approximately 80% of the variance of the original hyperspectral reflectance. The regression model with the first two principal components predicted a disease severity with RMSEs of 16.3% and 13.9% for the training and testing dataset, respec-tively. Partial least-square regression with seven extracted factors could most effectively predict disease severity compared with other statistical methods with RMSEs of 4.1% and 2.0% for the training and testing dataset, respectively. Our research demon-strates that it is feasible to estimate the disease severity of rice brown spot using hyperspectral reflectance data at the leaf level. | LIU Zhan-yu1, HUANG Jing-feng1, SHI Jing-jing1, TAO Rong-xiang2, ZHOU Wan3, ZHANG Li-li3 (1Institute of Agriculture Remote Sensing and Information System Application, Zhejiang University, Hangzhou 310029, China) (2Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China) (3Plant Inspection Station of Hangzhou City, Hangzhou 310020, China) | 2007 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2007，8，10： | 13 |
| 16 | Information flow and controlling in regularization inversion of quantitative remote sensing显示文摘In order to minimize uncertainty of the inversed parameters to the largest extent by making full use of the limited information in remote sensing data, it is necessary to understand what the information flow in quantitative remote sensing model inversion is, thus control the information flow. Aiming at this, the paper takes the linear kernel-driven model inversion as an example. At first, the information flow in different inversion methods is calculated and analyzed, then the effect of information flow controlled by multi-stage inversion strategy is studied, finally, an information matrix based on USM is defined to control information flow in inversion. It shows that using Shannon entropy decrease of the inversed parameters can express information flow more properly. Changing the weight of a priori knowledge in inversion or fixing parameters and partitioning datasets in multi-stage inversion strategy can control information flow. In regularization inversion of remote sensing, information matrix based on USM may be a better tool for quantitatively controlling information flow. | YANG Hua, XU Wangli, ZHAO Hongrui, CHEN Xue & WANG Jindi Research Center for Remote Sensing and GIS, School of Geography, Beijing Normal University, State Key Laboratory of Remote Sensing Science, Beijing Key Laboratory for Remote Sensing of Environment and Digital Cities, Beijing 100875, China Department of Mathematics, Beijing Normal University, Beijing 100875, China | 2005 | Science China Earth Sciences2005，48，1： | 12 |
| 17 | Changes in serum and histology of patients with chronic hepatitis B after interferon alpha-2b treatment显示文摘AIM: Chronic hepatitis B is a serious health problem.Interferon has long been used to treat Chronic hepatitis B.To evaluate the effects of interferon on chronic hepatitis Bbetter, we designed the study to investigate the changes insera and liver histology of patients with chronic hepatitis Bafter interferon alpha-2b treatment.METHODS: Twenty-four patients with chronic hepatitis Bwere enrolled in this study. They all received interferon alpha-2b treatment as following: 3 million units, i.m.. t.i.w., for 18weeks. Sera of all patients were obtained respectively forevaluation of ALT, HBsAg, HBcAg, HBeAg, HBV DNA andTIMP-1 before and afterinterferon treatment, also a liverbiopsy pre- and post-treatment was performed forcomparison of HAI, HBsAg, HBcAg, HBeAg, TIMP-1 andactivated HSC in the liver tissue.RESULTS: Patients who had normalization of serum ALTand seroconversion of HBeAg and/or HBV DNA (blothybridization) after treatment were defined as responders.The response rate in this study group was 37.5 % (7/24).Compared to pretreatment, the serum HBV DNA and TIMP-1 decreased significantly (P<0.05), so did the HAI, HBo Ag,HBeAg, TIMP-1 and activated HSC (P<0.05).CONCLUSION: The significant decrease in HBV DNA insera, the seroconversion of HBeAg, and the decrease ofviral expression in liver indicated that interferon alpha-2btreatment can inhibit viral replication. The normalization ofALT in sera and the improvement of HAI in liver showedthat interferon alpha-2b can improve the liver histology ofpatients with chronic hepatitis B. At the same time, interferonalpha-2b treatment can reduce the TIMP-1 in serum andliver and decrease the number of activated HSC, which mayallievate or inhibit hepatic fibrosis. Although the responserate was unsatisfactory, interferon play a benefical role onpatients with chronic hepatitis B in other respects. We stillneed further studies to improve the therapy effects. | Hong-Lei Han Zhen-Wei Lang Department of Pathology,Beijing Youan Hospital,Beijing 100054,Beijng City,China | 2003 | World Journal of Gastroenterology2003，9，1： | 12 |
| 18 | Killing effect of TNF-related apoptosis inducing ligand regulated by tetracycline on gastric cancer cell line NCI-N87显示文摘AIM To clone the cDNA fragment of human TRAIL (TNFrelated apoptosis inducing ligand) into a tetracyclineregulated gene expression system, the RevTet-On system, transduce expression vectors into a gastric carcinoma cell line-NCl-N87 and examine the effects of controlled expression of TRAIL in vitro on the gastric carcinoma cells.METHODS The full-length cDNA of TRAIL was inserted into a vector under the control of the tetracyclineresponsive element (TRE) to obtain the plasmid pRevTRETRAIL, which was transfected into a packaging cell line PT67. In addition, vector pRev-Tet- On and pRevTRE were also transfected into PT67 separately. After hygromycin and G418 selection, the viral titer was determined. The medium containing retroviral vectors was collected and used to transduce a gastric carcinoma cell line NCI-N87.The resulting cell line NCI-N87-Tet-On-TRE-TRAIL and a control cell line, NCI-N87-Tet-On-TRE, were established.TRAIL expression in the cell line was induced by incubating cells with doxycycline (Dox), which is a tetracycline analogue. The killing effect on gastric carcinoma cells was analyzed after induction.RESULTS The recombinant plasmid pRev-TRE-TRAIL was constructed. After hygromycin or G418 selection, the producer cell lines PT67-TRE, PT67-TRE-TRAIL and PT67TetOn were obtained, with titers of about 108CFU@ L-1 By transducing NCI-N87 cells with retroviral vectors from these cell lines, stable cell lines NCI-N87-Tet-On-TRETRAIL (NN3T) and control cell line NCI-N87-Tet-On-TRE (NN2T) were established. The growth curves of the selected cell lines were the same with the wild type NCIN87. When Dox was added, cell death was obvious in the test groups (29% -77%), whereas no difference was observed in control and wild type cell lines. With the addition of a medium from the test group, human leukemia cell line Jurkat was activated till death (83%), indicating the secretion of active TRAIL proteins from the test cells to the medium.CONCLUSION With the use of the RevTet-On system, a regulated expression system for TRAIL was constructed.Using this system, the selected killing effect of TRAIL on gastric carcinoma cell line NCI-N87 could be observed. | Xiao-Chao Wei Xin-Juan Wang Kai-Chen Lei Zhang Yu Liang Xin-Li Lin Department of Biochemistry and Molecular Biology,Peking University Health Science Center,Beijing 100083,ChinaProtein Studies,Oklahoma Medical Research Foundation,Oklahoma City,OK 73104,USA | 2001 | World Journal of Gastroenterology2001，7，4： | 11 |
| 19 | ION HEATING PROCESS DURING PLASMA IMMERSION ION IMPLANTATION显示文摘The research on plasma immersion ion implantation has been conducted for a little over ten years. Much is needed to investigate including processing technlogy, plasma sheath dynamics, interaction of plasma and surface, etc. Of the processing methods elavated temperature technique is usually used in PIII to produce a thick modified layer by means of the thermal diffusion. Meanwhile plasma ion heating is more recently developed by Ronghua Wei et al[1]. Therefore the temeperature is a critical parameter in plasma ion processing. In this paper we present the theoretical model and analysize the effect of imlantation voltage, plasma density, ion mass,etc on the temperature rise. | X.B. Tian, X.F. Wang, A.G. Liu, L.P. Wang, S. Y. Wang, B. Y. Tang and P. K. Chu 1)Advanced Welding Production & Technology National Key Laboratory, Harbin Institute of Technology, Harbin 150001, China 2)Department of Physics & Materials Science, City Uni | 2000 | Acta Metallurgica Sinica(English Letters)2000，13，2： | 11 |
| 20 | Study on NDVI-T_s space by combining LAI and evapotranspiration显示文摘This paper focuses on interpreting the different spatial relationships between NDVI and Ts, a triangular or a trapezoid, and on analyzing transformation conditions, the physical and ecological meanings of the vegetation index-surface temperature space as well. Further, we use the Tempera-ture-Vegetation Dryness Index (TVDI) to explain the existent meaning of a triangular space after NDVI reaches its saturated state by employing the relationships between NDVI, LAI and evapotranspiration. The specific relations between NDVI and Ts are useful for describing, validating and updating land surface models. | HAN Lijuan1,2,3, WANG Pengxin4, YANG Hua1,2,3, LIU Shaomin1,2,3 & WANG Jindi1,2,3 1. Research Center for Remote Sensing and GIS, School of Geography, Beijing Normal University, Beijing 100875, China 2. State Key Laboratory of Remote Sensing Science, Beijing 100875, China 3. Beijing Key Laboratory for Remote Sensing of Environment and Digital Cities, Beijing 100875, China 4. Department of Information Management, College of Information and Electronic Engineering, China Agricultural University, Beijing 100083, China | 2006 | Science China Earth Sciences2006，49，7： | 11 |
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