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| 1 | Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR显示文摘In order to study E. coli aerosol spreading from chicken houses to their surrounding air, air samples, including indoor and outdoor air (upwind 10 and 50 m as well as downwind 10, 50, 100, 200 and 400 m away) of 5 chicken houses were collected using six-stage Andersen microbial samplers and Reuter-Centrifugal samplers (RCS). E. coli concentrations (CFU/m3 air) collected from different sampling sites were calculated. E. coli strains from chicken feces samples were also isolated. Furthermore, the enterobacterial repetitive intergenic consensus (ERIC)-PCR method was applied to amplify the isolated E. coli strain DNA samples. Through the genetic similarity analyses of the E. coli obtained from different sampling sites, the spreading of bioaerosol from animal houses to the ambient air was characterized. The results showed that the isolated E. coli concentrations in indoor air (9―63 CFU/m3) in 5 chicken houses were higher than those in upwind and downwind air, but there were no significant differences between the indoor and downwind sites 10 m away from all the 5 houses (P>0.05). The phylogenetic tree indicated that a part of the E. coli (34.1%) isolated from indoor air had 100% similarity with those isolated from feces, and that most of E. coli isolated (54.5%) from downwind at 10, 50, 100 or even 200 m had 100% similarity with those isolated from indoor air or feces too. But those isolated from upwind air had a lower similarity (73%―92%) with corresponding strains isolated from indoor air or feces. Our results suggested that some strains isolated from downwind air and indoor air originated in the chicken feces, but most of isolates obtained from upwind air samples did not come from the chicken feces or indoor air. Effective hygienic measures should be taken in animal farms to prevent or minimize downwind spreading of microorganism aerosol. | DUAN HuiYong CHAI TongJie CAI YuMei ZHONG ZhaoBing YAO MeiLing ZHANG XingXiao | 2008 | Science China(Life Sciences)2008，51，2： | 5 |
| 2 | Concentration of airborne endotoxins and airborne bacteria in Chinese rabbit houses显示文摘 | Duan HuiYong Chai TongJie | 2006 | Berliner und Munchener Tierarztliche Wochenschrift2006，119，： | 1 |
| 3 | Vorkommen von Iuftgetragenen Keimen in Tierstaellen und der Stallumgebung显示文摘 | Chai Tongjie | 1999 | Shaker Verlag1999，56，2： | 1 |
| 4 | Formation and transmission of Staphylococcus aureus (including MRSA) aerosols carrying antibiotic-resistant genes in a poultry farming environment 显示文摘 | Liu Dunjiang Chai Tongjie Xia Xianzhu | 2012 | Sci Total Environ2012，426，： | 1 |
| 5 | Development of a realtime RT-PCR method for rapid detection of H9 avian influenza virus in the air显示文摘 | Jing Lv Baozhi Wei Tongjie Chai | | 0，，： | 1 |
| 6 | Simultaneous detection of airborne aflatoxin, ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography显示文摘 | WANG Yaling CHAI Tongjie LU Guozhong | 2008 | Environmental Research2008，107，： | 1 |
| 7 | 查看详情显示文摘 | Chai Tongjie Mueller W Zucker B-A | | 0，，： | 1 |
| 8 | ASYMPTOTICS OF THE RESIDUALS DENSITY ESTIMATION IN NONPARAMETRIC REGRESSION UNDER m(n)-DEPENDENT SAMPLE显示文摘Abatract | QIN GENGSHENG SHI SUNJUAN CHAI GENXIANG Department of Mathematics, Sichuan University Chengdu 610064 Department of Mathematics, Sichuan Educational College, Chengdu 610061 Department of Applied Mathematics, Tongji University Shanghai 200092. | 1996 | Applied Mathematics(A Journal of Chinese Universities)1996，11，1： | 0 |
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