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| 1 | 埃及伊蚊中肠上皮细胞的原代培养和细胞的持续培养(英文)显示文摘目的 探讨蚊虫中肠上皮细胞的培养 ,建立细胞的原代培养和细胞持续培养。 方法 用含抗生素的 10 %蔗糖水喂养新羽化出的埃及伊蚊成蚊 ,3d后 ,在无菌的条件下解剖中肠 ,用含抗生素的平衡盐溶液洗 3次 ,放入Grace’s培养基中 (含有 7%小牛血清 ,0 .5 %庆大霉素 ,1mg/ml抗酵母菌素和 6μl/ml复合维生素 ) ,2 5℃培养箱中培养。 结果 培养 3d后 ,95 %的细胞生长良好 ,7d后 ,大约 2 5 %的细胞死亡。第 3d的细胞死亡率明显低于以后的任何时间 (P <0 .0 5 ) ,从第 7d到 3 5d细胞死亡率差异无显著性 (P >0 .0 5 )。成功地建立起中肠上皮细胞的原代培养并能持续存活 40多天 ,保持细胞的连续培养。 结论 所发展的埃及伊蚊中肠上皮细胞的原代培养和细胞的持续培养的方法是成功的。 | 公茂庆 黄勇 谭文彬 Brian D.For John C.Beier | 2003 | 中国寄生虫病防治杂志2003，16，6： | 3 |
| 2 | Hidden reservoir of resistant parasites:the missing link in the elimination of falciparum malaria显示文摘Background:To successfully eliminate malaria,an integrated system that includes a number of approaches and interventions-aimed at overcoming the threat of antimalarial drug resistance-is required.Significant progress has been made in reducing malaria incidence through large-scale use of artemisinin-based combination therapies and insecticide-treated nets.To consolidate these gains,attention should be paid to the missing links in the elimination of malaria.One of these gaps is the residual reservoir of submicroscopic resistant parasites,which remains after case management or other control measures have been carried out.Therefore,the present opinion piece highlights the importance of exploring the role that submicroscopic resistant parasites could play in hindering malaria elimination by allowing the persistence of transmission,particularly in areas of low transmission or in the pre-elimination and/or elimination phase.Discussion:If malaria elimination interventions are to be effective,the relative role of the hidden reservoir of resistant parasites needs to be assessed,particularly in regions that are low-transmission settings and/or in pre-elimination and/or elimination phases.Various ongoing studies are focusing on the role of submicroscopic malaria infections in malaria transmission but overlook the possible build-up of resistance to antimalarial drugs among submicroscopic parasite populations.This is an important factor as it may eventually limit the effectiveness of malaria elimination strategies.Conclusions:An evidence-based estimation of the“true”reservoir of resistant parasites can help target the existing and emerging foci of resistant parasites before they spread.Emergence and spread of artemisinin-resistant Plasmodium falciparum malaria in Southeast Asia underline the need to contain drug resistance. | Rashad Abdul-Ghani Mohammed A.K.Mahdy John C.Beier Leonardo K.Basco | 2017 | Infectious Diseases of Poverty2017，6，1： | 1 |
| 3 | 北极苔原和森林地区的稳定性及脆弱性与社会和环境变化显示文摘北极苔原带和北方森林(北部地区)因其分布广阔并远离农业土地利用变化及工业发展的影响,而一度被认为是地球上最后的净土。但如今这些地区如同地球上任何别的地区一样,也正经受着环境和社会变化的影响。本文总结了北部地区在全球系统中所起的作用,并提出方案以便对导致该地区对社会和环境变化敏感的那些因子进行评价。 | F.S.Chapin Ⅲ G.Peterson F.Berkes T.V.Callaghan P.Angelstam M.Apps C.Beier Y.Bergeron A.-S.Crépin K.Danell T.Elmqvist C.Folke B.Forbes N.Fresco G.Juday J.Niemel A.Shvidenko G.Whiteman 肖飞 | 2004 | AMBIO-人类环境杂志2004，33，6： | 0 |
| 4 | 抗蚊媒中肠抗体对冈比亚按蚊生殖率和死亡率影响的研究(英文)显示文摘目的 探讨冈比亚按蚊中肠抗原抗媒介免疫的作用 ,进一步研究抗媒介疫苗抗原。 方法 解剖室内饲养的冈比亚按蚊雌蚊中肠 ,立即置于冷的 p H7.4 PBS溶液中 ,用 SDS- PAGE方法获得分子质量为 4 3ku中肠蛋白抗原 ,皮下注射免疫大白兔 3次 ,获得的免疫血浆膜饲冈比亚按蚊成蚊 ,与对照组比较 ,观察其对冈比亚按蚊死亡率和生殖率的影响。 结果 膜饲免疫血浆的冈比亚按蚊的死亡率与对照组相比显著增高 ,死亡率增加 5 6 % ;生殖率明显下降 ,实验组蚊虫平均产卵率为 71.4个 ,对照组为 88.1个 ,下降 18.7%。 | 公茂庆 黄勇 李士根 Brian D.Foy John C.Beier | 2002 | 中国寄生虫病防治杂志2002，15，4： | 0 |
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