|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 2 | AMP-activated kinase in human spermatozoa: identification, intracellular localization, and key function in the reRulation of sperm motility显示文摘 | Violeta Calle-Guisado Ana Hurtado de Llera David Martin-Hidalgo Jose Mijares Maria C Gil Ignacio S Alvarez Maria J Bragado Luis J Garcia-Marin | 2017 | Asian Journal of Andrology2017，19，6： | 5 |
| 3 | Mitochondrial Sulfide Detoxification Requires a Functional Isoform O-Acetylserine(thiol)lyase C in Arabidopsis thaliana显示文摘在 non-cyanogenic 种类,氰化物的主要来源源于乙烯和 camalexin biosyntheses。在线粒体,氰化物是细胞色素 c oxidase 的一个有势力禁止者并且被 -cyanoalanine synthase CYS-C1 使产生代射变化,催化到氢硫化物和 -cyanoalanine 的半胱氨酸和氰化物的变换。释放的氢硫化物也禁止细胞色素 c oxidase 并且需要被 O-acetylserine (thiol ) lyase mitochondrial isoform 除去, OAS-C,催化硫化物的加入到 O-acetylserine 生产半胱氨酸,因此在线粒体产生一条周期的小径。功能的 OAS-C isoforms 的损失引起 phenotypic 很类似于 CYS-C1 酶的损失的特征,显示出在根头发形成的缺点。有 OAS-C 基因的基因互补救根头发延伸的缺陷,恢复野类型的显型。线粒体妥协他们适当地除去的能力氰化物和产生硫化物因为后者不能在 oas-c 0 异种重新吸收进半胱氨酸。因而,我们观察硫化物和氰化物并且其他的 oxidase 的累积,它是不能的由于两个有毒的分子的累积可能阻止反应的氧种类的生产。我们的结果允许我们建议 OAS-C 的意义在线粒体与它在合适的硫化物和氰化物 detoxification 的角色有关。 | Consolacien Alvarez Irene Garcia Luis C Romero Cecilia Gotor | 2012 | Molecular Plant2012，5，6： | 2 |
| 4 | 3D additive manufactured composite scaffolds with antibiotic-loaded lamellar fillers for bone infection prevention and tissue regeneration显示文摘Bone infections following open bone fracture or implant surgery remain a challenge in the orthopedics field.In order to avoid high doses of systemic drug administration,optimized local antibiotic release from scaffolds is required.3D additive manufactured(AM)scaffolds made with biodegradable polymers are ideal to support bone healing in non-union scenarios and can be given antimicrobial properties by the incorporation of antibiotics.In this study,ciprofloxacin and gentamicin intercalated in the interlamellar spaces of magnesium aluminum layered double hydroxides(MgAl)andα-zirconium phosphates(ZrP),respectively,are dispersed within a thermoplastic polymer by melt compounding and subsequently processed via high temperature melt extrusion AM(~190◦C)into 3D scaffolds.The inorganic fillers enable a sustained antibiotics release through the polymer matrix,controlled by antibiotics counterions exchange or pH conditions.Importantly,both antibiotics retain their functionality after the manufacturing process at high temperatures,as verified by their activity against both Gram+and Gram-bacterial strains.Moreover,scaffolds loaded with filler-antibiotic do not impair human mesenchymal stromal cells osteogenic differentiation,allowing matrix mineralization and the expression of relevant osteogenic markers.Overall,these results suggest the possibility of fabricating dual functionality 3D scaffolds via high temperature melt extrusion for bone regeneration and infection prevention. | María C´amara-Torres Stacy Duarte Ravi Sinha Ainhoa Egizabal Noelia´Alvarez Maria Bastianini Michele Sisani Paolo Scopece Marco Scatto Alessandro Bonetto Antonio Marcomini Alberto Sanchez Alessandro Patelli Carlos Mota Lorenzo Moroni | 2021 | Bioactive Materials2021，6，4： | 2 |
| 5 | 显示文摘 | del Valle J A Picasso A C Alvarez I | 1999 | Scripta Materialia1999，41，3： | 1 |
| 6 | Use of Kraft black liquors from a pulp mill for the production of soil conditioners 显示文摘 | Alvarez R Coca J | 1992 | Water Air and Soil Pollution1992，61，： | 1 |
| 7 | Lipids,lipoproteins and apoproteins in serum during infection显示文摘 | Ramos A | 1986 | Clin Chem1986，32，1： | 1 |
| 8 | Prostaglandin E2 synergistically with interleukin-23 favors human Th17 expansion显示文摘 | Chizzolini C Chicheportiche R Alvarez M | 2008 | Blood2008，112，9： | 1 |
| 9 | Stereotactic biopsy for brainstem tumors in pediatric patients 显示文摘 | PEREZ GOMEZ J L RODRIGUEZ ALVAREZ C A MARHX BRACHO A | 2010 | Childs Nerv Syst2010，26，1： | 1 |
| 10 | X-ray photoelectron spectroscopy of amorphous AlN alloys prepared by reactive rf sputtering显示文摘 | RIBEIRO C T M ZANATTA A R ALVAREZ F | 2002 | Journal of Non-Crystalline Solids2002，，1： | 1 |
| 11 | Use of kraft black liquors from a pulp mill for the production of soil conditioner显示文摘 | GONZ C ALVAREZ R COCA J | 1992 | Waste Management and Research1992，10，2： | 1 |
| 12 | Biodegradation of diesel fuel-contaminated wastewater using a three-phase fluidized bed reactor显示文摘 | Lohi A Alvarez C M Anania G | 2008 | Journal of Hazardous Materials2008，154，13： | 1 |
| 13 | Soil organic matter pools and their associations with carbon mineralization kinetics 显示文摘 | Alvarez R Alvarez C R | 2000 | Soil Science Society of America Journal2000，64，： | 1 |
| 14 | SPARC endogenous level,rather than fibroblast-produced SPAR-C or stroma reorganization induced by SPARC,is responsible for mel-anoma cell growth显示文摘 | Prada F Benedetti LG Bravo AI Alvarez MJ Carbone C PodhajcerOL | 2007 | J Invest Dermatol2007，127，： | 1 |
| 15 | Current databases on biological variation: pros, cons and progress 显示文摘 | Ricos C Alvarez V Cava F | 1999 | Scand J Clin Lab Invest1999，59，： | 1 |
| 16 | Soil organic matter pools and their associations with carbon mineralization kinetics 显示文摘 | Alvarez R Alvarez C R | 2000 | Soil Science Society of America Journal2000，64，1： | 1 |
| 17 | Economic, social and envi- ronmental impact of land consolidation in Galicia 显示文摘 | Crecente R Alvarez C Frau U | 2002 | Land Use Policy2002，，19： | 1 |
| 18 | Alterations ofdopaminergic and noradrenergic innerrations in mortor cortex inParkinson's disease显示文摘 | Gaspar P Puyckaerts C Alvarez C | 1991 | Ann Neurol1991，30，： | 1 |
| 19 | Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal 显示文摘 | Aldea C Alvarez C P Folgueira L | 2002 | J Clin Microbiol2002，40，3： | 1 |
| 20 | Evaluation of durability tests for permeable friction course mixtures 显示文摘 | Alvarez A E Epps-Martin A Estakhri C | 2010 | International Journal of Pavement Engineering2010，11，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员