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| 1 | Current progress in use of adipose derived stem cells in peripheral nerve regeneration显示文摘Unlike central nervous system neurons; those in the peripheral nervous system have the potential for full regeneration after injury. Following injury, recovery is controlled by schwann cells which replicate and modulate the subsequent immune response. The level of nerve recovery is strongly linked to the severity of the initial injury despite the significant advancements in imaging and surgical techniques. Multiple experimental model shave been used with varying successes to augment the natural regenerative processes which occur following nerve injury. Stem cell therapy in peripheral nerve injury may be an important future intervention to improve the best attainable clinical results. In particular adipose derived stem cells(ADSCs) are multipotent mesenchymal stem cells similar to bone marrow derived stem cells, which are thought to have neurotrophic properties and the ability to differentiate into multiple lineages. They are ubiquitous within adipose tissue; they can form many structures resembling the mature adult peripheral nervous system. Following early in vitro work; multiple small and large animal in vivo models have been used in conjunction with conduits, autografts and allografts to successfully bridge the peripheral nerve gap. Some of the ADSC related neuroprotective and regenerative properties have been elucidated however much work remains before a model can be used successfully in human peripheral nerve injury(PNI). This review aims to provide a detailed overview of progress made in the use of ADSC in PNI, with discussion on the role of a tissue engineered approach for PNI repair. | Shomari DL Zack-Williams Peter E Butler Deepak M Kalaskar | 2015 | World Journal of Stem Cells2015，7，1： | 8 |
| 2 | DOTAP脂质体介导VEGF基因转染人脂肪干细胞及目的基因的表达显示文摘目的:研究脂质体介导的血管内皮生长因子(VEGF)基因转染人脂肪干细胞(hASCs)及VEGF的表达。方法:胶原酶消化吸脂术脂肪,收获细胞沉淀,培养至第4代时利用流式细胞术检测细胞表型,并诱导其成脂成骨分化,鉴定为hASCs。转染组利用DOTAP脂质体将pIRES2-EGFP-VEGF质粒转入hASCs,对照组为脂质体空载。免疫荧光染色检测hASCs内VEGF的表达,ELISA检测上清液中VEGF浓度的变化。结果:1ml吸脂术脂肪可得到(4.38±0.21)×10^5个细胞,第4代时hASCs高表达CD90(81.49%),低表达CD19(6.37%)、CD31(14.91%)、CD34(17.56%)和CD45(15.39%),诱导分化后油红O和茜素红染色均呈阳性。分光光度计显示质粒DNA浓度为595ng/μl。转染组hASCs表达GFP和VEGF,转染效率为(43.69±18.53)%;对照组不表达GFP,但表达较低的VEGF。转染组VEGF的光密度是对照组的2.13倍,其上清液中的VEGF浓度随时间显著上升,与对照组相比差异有统计学意义(P〈0.05)。结论:DOTAP脂质体可成功将pIRES2-EGFP-VEGF质粒转入hASCs,转染后的VEGF表达和分泌水平显著提高。 | 陈犹白 张启旭 Butler C E 吴叶文 张丽萍 董江陵 陈聪慧 韩岩 | 2016 | 临床耳鼻咽喉头颈外科杂志2016，30，12： | 6 |
| 3 | Control of stem cell fate by engineering their micro and nanoenvironment显示文摘Stem cells are capable of long-term self-renewal and differentiation into specialised cell types, making them an ideal candidate for a cell source for regenerative medicine. The control of stem cell fate has become a major area of interest in the field of regenerative medicine and therapeutic intervention. Conventional methods of chemically inducing stem cells into specific lineages is being challenged by the advances in biomaterial technology, with evidence highlighting that material properties are capable of driving stem cell fate. Materials are being designed to mimic the clues stem cells receive in their in vivo stem cell niche including topographical and chemical instructions. Nanotopographical clues that mimic the extracellular matrix(ECM) in vivo have shown to regulate stem cell differentiation. The delivery of ECM components on biomaterials in the form of short peptides sequences has also proved successful in directing stem cell lineage. Growth factors responsible for controlling stem cell fate in vivo have also been delivered via biomaterials to provide clues to determine stem cell differentiation. An alternative approach to guide stem cells fate is to provide genetic clues including delivering DNA plasmids and small interfering RNAs via scaffolds. This review, aims to provide an overview of the topographical, chemical and molecular clues that biomaterials can provide to guide stem cell fate. The promising features and challenges of such approaches will be highlighted, to provide directions for future advancements in this exciting area of stem cell translation for regenerative medicine. | Michelle F Griffin Peter E Butler Alexander M Seifalian Deepak M Kalaskar | 2015 | World Journal of Stem Cells2015，7，1： | 3 |
| 4 | Evidence for a 15; 17translocation in every patient with acute promyelmytic leukemia显示文摘 | LARSON R A KONDO K VARDIMAN J W BUTLER A E GOLOMB H M ROWLEY J D | 1984 | Am J Med1984，76，5： | 1 |
| 5 | Vanadium peroxide complexes显示文摘 | Butler A Clague M J Meister G E | 1994 | Chem Rev1994，94，3： | 1 |
| 6 | A segmentation analysis of resident attitudes to the social impact of tourism显示文摘 | Brougham J E Butler R W | 1981 | Annals of Tourism Research1981，8，4： | 1 |
| 7 | Expressed sequenced tag-based gene expression analysis under aluminum stress in rye显示文摘 | Rodriguez M A R Butler E Huete A R | 2002 | Plant physiology2002，130，： | 1 |
| 8 | 显示文摘 | Butler P E Cao Y L | 1998 | Plast Reconstr Swry1998，10，6： | 1 |
| 9 | Characterizing perflu- orooctanoate in ambient air near the fence line of a manufacturing facility : Comparing modeled and monitored values 显示文摘 | Barton C Butler L E Zarzecki C J | 2006 | Journal of Air Waste Managenvent Association2006，56，1： | 1 |
| 10 | An adjuvant for mucosal and systemic immunity 显示文摘 | BUTLER J E | 1992 | Fluoride1992，25，2： | 1 |
| 11 | Extensive cell en- velope modulation is associated with virulence in Brucella abortus 显示文摘 | Lamontagne J Butler H Chaves-Olarte E | 2007 | J Proteome Res2007，6，4： | 1 |
| 12 | Investigating the nucleic acid interaction andantimicrobial mechanism of buforin H 显示文摘 | Uyterhoeven E T Butler C H Danette K | 2008 | FEBSLetters2008，582，12： | 1 |
| 13 | Cimateral induced musclehypertrophy and altered endocrine status in Iambs显示文摘 | BEATMAUN D J BUTLER W R HOGUE D E | 1997 | J Anita Sci1997，65，10： | 1 |
| 14 | Innovate with complexinformation technologies : A theoretical model and empirical examina-tion 显示文摘 | WANG W BUTLER J E HSIEH J J | 2008 | Journal of Computer Information Systems2008，49，1： | 1 |
| 15 | Treatment of myelopathyin Sjogren syndrome with a combination of prednisone andcyclophosphamide显示文摘 | Williams CS Butler E Roman GC | 2001 | Arch Neurol2001，58，5： | 1 |
| 16 | Network evolution, entre- preneurial success, and regional development 显示文摘 | Butler J E Hansen G S | 1999 | Entrepreneurship & Regional Development1999，，1： | 1 |
| 17 | Ligament restraints to anterior pisterior drawer in the human knee:a biomechanicals tudy 显示文摘 | BUTLER D L NOYER F R GROOD E S | 1980 | Bone joint SurhAm1980，62，： | 1 |
| 18 | Genetic Characterization of a Single Bifunctional Enzyme for Fumarate Reduction and Succinate Oxidation in Geobacter sulfurreducens and Engineering of Fumarate Reduction in Geobacter metallireducens显示文摘 | Butler J E Glaven R H Esteve-Nú(n)ez A | 2006 | Journal of Bacteriology2006，188，2： | 1 |
| 19 | Electron transmission studies of diamond films显示文摘 | Yater J E Shih A Butler P E | 2002 | Appl Surf Sci2002，191，14： | 1 |
| 20 | Precipitation of sulfides from homogeneous solutions by thioacetamide显示文摘 | Swift E Butler E | 1956 | Anal Chem1956，28，2： | 1 |
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