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| 1 | 唐氏综合征动物模型肠神经系发育的动态观察显示文摘目的通过研究唐氏综合征动物模型16三体鼠肠神经系发育,揭示16三体鼠是否伴有先天性巨结肠。方法通过免疫组织化学方法测定16三体胎鼠及同龄胎鼠蛋白基因产物9.5(PGP9.5),并观察了肠神经系的形态学特征。结果16三体胎鼠肠神经系发育异常,肠肌间神经丛发育迟缓,肠粘膜下神经丛缺如,盲肠末端有5mm的无神经节区。但肠道外源性神经支配正常,肠系膜神经系发育良好。结论唐氏综合征动物模型16三体鼠伴有先天性巨结肠,这种动物模型可用于研究先天性巨结肠病。 | 李继承 Busch,LC | 1999 | 中华医学杂志1999，79，6： | 3 |
| 2 | The development of colon innervation in trisomy mice and Hirschsprungs disease显示文摘AIM To study the colon innervation of trisomy16 mouse, an animal model for Downssyndrome, and the expression of protein geneproduct 9.5 ( PGP 9.5) in the stenosed segmentof colon in Hirschsprungs disease (HD).METHODS Trisomy 16 mouse breeding;cytogenetic analysis of trisomy 16 mice; andPGP 9.5 immunohistochemistry of colons oftrisomy 16 mice and HD were carried out.RESULTS Compared with their normalIittermates, the nervous system of colon intrisomy 16 mice was abnormally developed.There existed developmental delay of muscularplexuses of colon, no submucosal plexus wasfound in the colon, and there was 5mmaganglionic bowel aparting from the anus intrisomy 16 mice. The mesentery nerve fiberswere as well developed as shown in their normallittermates. Abundant proliferation of PGP 9.5positive nerve fibers was revealed in thestenosed segment of HD colon.CONCLUSION Trisomy 16 mice could serve asaganglionic bowel in the distal part of colon.Abundant proliferation of PGP 9.5 positive fibersresulted from extrinsic nerve compensation,since no ganglionic cells were observed in thestenosed segment of the colon in HD. HD has agenetic tendency. | LC Busch W Kuhnel | 2001 | World Journal of Gastroenterology2001，7，1： | 3 |
| 3 | Anatomy of the coracohnmeral and coraco-glenoidal ligaments 显示文摘 | Kohs I Busch LC Tomusk H | 2000 | Ann Anat2000，182，6： | 1 |
| 4 | Immunohistochemical study on gastroenteric nervous system in trisomy 16 mice:an animal model of Down syndrome显示文摘AIM To study the development of gastroentericnervous system in trisomy 16 mouse embryos.The gastroenteric nervous system in trisomy 16mice and their normal littermates,serving ascontrols from embryonic days 13 to 18(ED13-18)was identified by using primary antibody againstprotein gene product(PGP)9.5.METHODS Trisomy 16 mouse breeding andtrisomy 16 mouse embryos were identified fromtheir normal littermates by chromosomeexamination;PGP 9.5 immunohistochemicalstainning.CONCLUSION Trisomy 16 mice, as an animal model for Down syndrome, has abnormality not only in several systems and organs but also in gastroenteric innervation. This report describes for the first time that the development of the gastroenteric nervous system was not only delayed but also pathological. | LC Busch W Kuhnel | 2000 | World Journal of Gastroenterology2000，6，6： | 1 |
| 5 | 常染色体16三体胎鼠和正常同窝鼠肠神经系发育显示文摘目的 研究 Down综合征动物模型常染色体 16三体胎鼠及其正常同窝鼠肠神经系 (enteric nervoussystem,ENS)发育状况。方法 (1)将 NMR- 1雌鼠与携带有两个 Robertsonian(Rb)易位 (11,16 ) ,Rb(16 ,17)8L ubt WL ub3雄鼠进行交配 ,繁殖常染色体 16三体胎鼠。 (2 )采用细胞遗传学分析 ,以检出两条 Rb染色体和 41条染色体者定为常染色体 16三体胎鼠。 (3)采用高度特异的神经标记物蛋白基因产物 9.5 (protein gene product 9.5 ,PGP 9.5 )抗体进行免疫组织化学染色。结果 在胚胎期第 13天 (em bryonic days,ED13) ,常染色体 16三体胎鼠和正常同窝鼠的肠壁内均未发现有神经元存在。 ED14时 ,正常同窝鼠肠壁内可见散在分布的神经元 ;至 ED15时 ,散在分布的神经元胞体间以突起相连 ,形成原始肌间神经丛 ;ED16时 ,肌间神经丛呈网络状 ,具有发育良好的神经节。而 ED14~ ED16时的常染色体 16三体胎鼠肠壁仅发现散在分布的神经元胞体。 ED17和 ED18时 ,正常同窝鼠ENS发育良好 ,肌间神经丛形成 ,且分布广泛 ,排列成规则的神经网络 ,粘膜下神经丛形态不规则 ,两丛间有广泛的神经纤维联系。同胎龄期常染色体 16三体胎鼠 ENS发育迟缓 ,粘膜下神经丛缺如 ,后肠末端有 5 mm无神经节的肠管。从 ED18~ ED14常染色体 16? | 李继承 LC Busch W Kühnel | 2000 | 中国医学科学院学报2000，22，2： | 0 |
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