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| 1 | NT157 has antineoplastic effects and inhibits IRS1/2 and STAT3/5 in JAK2^(V617F)-positive myeloproliferative neoplasm cells显示文摘Recent data indicate that IGF1R/IRS signaling is a potential therapeutic target in BCR-ABL1-negative myeloproliferative neoplasms(MPN);in this pathway,IRS2 is involved in the malignant transformation induced by JAK2^(V617F),and upregulation of IGF1R signaling induces the MPN phenotype.NT157,a synthetic compound designed as an IGF1R-IRS1/2 inhibitor,has been shown to induce antineoplastic effects in solid tumors.Herein,we aimed to characterize the molecular and cellular effects of NT157 in JAK2^(V617F)positive MPN cell lines(HEL and SET2)and primary patient hematopoietic cells.In JAK2^(V617F)cell lines,NT157 decreased cell viability,clonogenicity,and cell proliferation,resulting in increases in apoptosis and cell cycle arrest in the G2/M phase(p<0.05).NT157 treatment inhibited IRS1/2,JAK2/STAT,and NFκB signaling,and it activated the AP-1 complex,downregulated four oncogenes(CCND1,MYB,WT1,and NFKB1),and upregulated three apoptotic-related genes(CDKN1A,FOS,and JUN)(p<0.05).NT157 induced genotoxic stress in a JAK2/STAT-independent manner.NT157 inhibited erythropoietin-independent colony formation in cells from polycythemia vera patients(p<0.05).These findings further elucidate the mechanism of NT157 action in a MPN context and suggest that targeting IRS1/2 proteins may represent a promising therapeutic strategy for MPN. | Bruna Alves Fenerich Jaqueline Cristina Fernandes Ana Paula Nunes Rodrigues Alves Juan Luiz Coelho-Silva Renata Scopim-Ribeiro Priscila Santos Scheucher Christopher A.Eide Cristina E.Tognon Brian J.Druker Eduardo Magalhães Rego João Agostinho Machado-Neto Fabiola Traina | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 2 | Induction of Sensitivity to ganciclovir in human hepatocellular Carcinoma cells by adenovirus - mediated gene transfer of herpes simplex virus thymidine Kinase显示文摘 | Bilbao R Bruna O | 1995 | Hepatology1995，22，： | 1 |
| 3 | Induction of sensitivity to ganciclovir in human hepatocellular carcinoma cells by adenovirus-mediated gene transfer of herpes simplex virus thymidine kinase显示文摘 | Qian C Bilbao R Bruna O | 1995 | Hepatology1995，22，1： | 1 |
| 4 | Changes in the components of dry-fermented sausages during ripening显示文摘 | ORD(O)(N) EZ J A HIERRO E M BRUNA J M | 2010 | Food Science and Nutrition2010，39，4： | 1 |
| 5 | Induction of sensitivity to ganciclovir in human hepatocellular carcinoma cells by adenovirus mediated gene transfer ofherps simplex virus thymidine kinase 显示文摘 | Qian c Bilbao R Bruna O | 1995 | Hepatology1995，22，1： | 1 |
| 6 | Induction of sensitivity to ganciclovir in human hepatocellular carcinoma cells by adenovirus-mediated gene transfer of herpes simplex virus thymidine kinase显示文摘 | Qian C Bilbao R Bruna O | 1995 | Hepatology1995，22，1： | 1 |
| 7 | The Colider and Roosevelt olcanic rocks (sw amazonian craton): geochemistry and sm-nd isotope characteristics of a silicic large igneous province显示文摘The volcanic rocks of the Colíder and Roosevelt formations are extensively exposed in the south-central portion of the Amazonian Craton where effusive and pyroclastic rocks have been mapped.Both units,topped by chemical sediments and oceanic facies as rhyolite and andesite lavas,rhyodacite,and porphyritic dacite,with frequent intercalations of pyroclastic and epiclastic deposits.Whole-rock geochemistry for 55 samples of rhyolitic to andesitic composition suggests the involvement of fertile mantle-derived components with E-MORB to OIB compositions.The analyzed rocks display calc-alkaline to shoshonitic affinity consistent with generation related to an active continental margin.The whole-rock Sm-Nd isotope data from selected felsic volcanic rocks of the Colíder and Roosevelt formations yield negative initial eNd values between–3 and–9,indicating the predominantly crustal nature of the parental magmas with early Archean to late Paleoproterozoic(ca.2.5–2.0 Ga)depleted mantle model ages. | Bruna Saar de Almeida Mauro César Geraldes Carlos Augusto Sommer Felipe Corrales Antonio Joāo Paes de Barros | 2021 | Acta Geochimica2021，40，6： | 1 |
| 8 | 查看详情显示文摘 | Fernanda V M Pontes Bruna A de O Mendes Evelyn M F de Souza | | 0，，2： | 1 |
| 9 | 显示文摘 | Bilbao R Bruna O | 1995 | Hepatology1995，22，1： | 1 |
| 10 | Harmine and Imipramine Promote Antioxidant Activities in Prefrontal Cortex and Hippocampus显示文摘 | Gislaine Z. Réus Roberto B. Stringari Bruna de Souza Fabrícia Petronilho Felipe Dal-Pizzol Jaime E. Hallak Ant?nio W. Zuardi José A. Crippa Jo?o Quevedo | 2010 | Oxidative Medicine and Cellular Longevity2010，，5： | 1 |
| 11 | Vaccination With Replication -deficient Recombinant Adenoviruses Encoding the Main Surface Antigens of Toxoplasma gondii Induces Immune Response and Protection Against Infection in Mice 显示文摘 | Caetano B C Bruna - Romero O Fux B | 2006 | Hum Gene Ther2006，17，4： | 1 |
| 12 | Induction of sensitivity to ganciclovir in human hepatocellular carcinoma cells by adenovirus-mediated gene transfer of herpes simplex virus thymidine kinase显示文摘 | Qian C Bilbao R Bruna O | 1995 | Hepatology1995，22，1： | 1 |
| 13 | DLST mutations in pheochromocytoma and paraganglioma cause proteome hyposuccinylation andmetabolic remodeling显示文摘Dear Editor,Of all human tumors,pheochromocytomas and paragangliomas(PPGLs)have the highest heritability rate.Over 15%of PPGLs harbor mutations in genes encoding tricarboxylic acid(TCA)cycle-related enzymes that cause oncometabolite accumulation and drive tumorigenesis via metabolic adaptation to hypoxia and global hypermethylation[1].The dihydrolipoamide S-succinyltransferase(DLST)gene was recently described as a new PPGL susceptibility gene[2]. | Sara Mellid Fernando García Luis Javier Leandro-García Alberto Díaz-Talavera Ángel Mario Martínez-Montes Eduardo Gil Bruna Calsina María Monteagudo Rocío Letón Juan María Roldán-Romero María Santos Javier Lanillos Carlos Valdivia Natalia Martínez-Puente Javier de Nicolás-Hernández Scherezade Jiménez Manuel Pérez-Martínez Emiliano Honrado Javier Coloma Ana Cerezo Clara María Santiveri Manel Esteller Ramón Campos-Olivas Eduardo Caleiras Cristina Montero-Conde Cristina Rodríguez-Antona Javier Muñoz Mercedes Robledo Alberto Cascón | 2023 | Cancer Communications2023，43，7： | 0 |
| 14 | Transcranial direct current electrical stimulation in combination with aerobic exercise:A pilot study in post-COVID-19 systemic autoimmune rheumatic patients显示文摘BACKGROUND Systemic autoimmune rheumatic diseases(SARDs)are a group of diseases with multiorgan involvement and a high prevalence of chronic pain and fatigue.Patients with SARDs and post-coronavirus disease 2019(COVID-19)syndrome experience aggravation of symptoms.In this context,it is essential to establish strategies to reduce chronic pain and fatigue and improve quality of life.AIM To assess the efficacy of transcranial direct current stimulation(tDCS)for the treatment of fatigue and pain-associated post-COVID-19 syndrome in patients with SARDs.METHODS This study included nine patients with different types of SARDs.All patients had reverse transcription-polymerase chain reaction(RT-PCR)test confirmed COVID-19 as well as significant,persistent fatigue and pain that began to worsen after infection.Anodal tDCS was administered in five daily sessions(2mA,20 min).Concomitantly,patients were involved in aerobic exercise program.All participants were evaluated using specific questionnaires and strength assessment by handgrip and physical function by timed-up-and-go test and sit-to-stand test at baseline(within one week before tDCS protocol),and one week after tDCS protocol.During all procedures,the patients’treatments remained unchanged.RESULTS The sample comprised eight women and one man with a mean age of 48.7±9.6 years.After the tDCS protocol,pain and fatigue significantly improved on the visual analog scale(P<0.05).The physical function also improved 9.5±2.7 vs 6.8±0.8(P=0.001)for timed-up-go-test and 10.3±3.7 vs 15.1±4.0(P=0.037)for sit-to-stand test.None of the patients experienced any adverse events.CONCLUSION The present study showed that tDCS in combination with aerobic exercise was effective in improving physical function,and reducing fatigue/pain in SARDs patients with post-COVID-19 syndrome. | Rafael Giovani Missé Alexandre Moura dos Santos Isabela Bruna Pires Borges Marlise Sítima Mendes Simões Lorenza Rosa Silvério Bruna Lindoso Correia Ana Wook Sook Kim Aline Marques Caetano Sandra Gofinet Pasoto Carla Gonçalves Schahin Saad Diogo Souza Domiciano Clarice Tanaka Julia Maria D’Andrea Greve Abrahão Fontes Baptista Samuel Katsuyuki Shinjo | 2023 | World Journal of Rheumatology2023，11，1： | 0 |
| 15 | Perspective on the use of synthetic biology in rudimentary food fermentations显示文摘Rudimentary food fermentation can be defined as a spontaneous process of conversion of food components through enzy-matic action.A great variety of fermented foods are produced using spontaneous approaches;however,cocoa and coffee represent the most important agricultural commodities on international markets.As a manner to increase the efficiency of these processes,starter cultures have been developed and applied under field conditions.The selection process begins with the recovery of microbial strains from spontaneous fermentation through phenotypic and metabolic traits.Next,mutation-based breeding is used to develop and improve well-adapted starter cultures.With advances in synthetic biology,especially in the last decade,the development of robust cellular fabrications with high fermentative capacity has become easier-largely due to the development of genomic approaches,such as next-generation sequencing techniques,CRISPR-Cas system and bioinformatics tools.This review brings prospects on the use of synthetic biology to design new robust strains for use in cocoa and coffee fermentations,but which can be extended to other rudimentary foods.In addition,metabolic traits and target genes(e.g.,UvrA,RecA,GPD1,and GPP2)are proposed as a starting point for the improvement of cocoa and coffee starters.Finally,the regulatory and safety requirements for these food crops are addressed.This review aims to stimulate research on the process of fermentation and the associated synthetic biology tools to produce fermented food efficiently and sustainably. | Alexander da Silva Vale Gilberto Vinícius de Melo Pereira Leonardo Martins Santana Dão Pedro de Carvalho Neto Brigitte Sthepani Orozco Colonia Vanete Thomaz Soccol Bruna Leal Maske Carlos Ricardo Soccol | 2023 | Systems Microbiology and Biomanufacturing2023，3，1： | 0 |
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