|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Facile construction of carbon dots via acid catalytic hydrothermal method and their application for target imaging of cancer cells显示文摘为了解决高温度或长反应的问题,在碳点(CD ) 的热水的合成预定,一个新奇方法基于支持的碳化由盐酸催化作用在现在的工作被开发的酸。催化的碳点的酸(ACD ) 在 200 虪箳椰嬑郢 G | Zhenggang Wang Boshi Fu Siwei Zou Bo Duan Chunyu Chang Bai Yang Xiang Zhou Lina Zhang | 2016 | Nano Research2016，9，1： | 8 |
| 2 | Method for the extraction of circulating nucleic acids based on MOF reveals cell-free RNA signatures in liver cancer显示文摘Cell-free RNA(cf RNA) allows assessment of health, status, and phenotype of a variety of human organs and is a potential biomarker to non-invasively diagnose numerous diseases. Nevertheless, there is a lack of highly efficient and bias-free cf RNA isolation technologies due to the low abundance and instability of cf RNA.Here, we developed a reproducible and high-efficiency isolation technology for different types of cell-free nucleic acids(containing cf RNA and viral RNA) in serum/plasma based on the inclusion of nucleic acids by metal-organic framework(MOF) materials, which greatly improved the isolation efficiency and was able to preserve RNA integrity compared with the most widely used research kit method. Importantly, the quality of cf RNA extracted by the MOF method is about 10-fold that of the kit method, and the MOF method isolates more than three times as many different RNA types as the kit method. The whole transcriptome mapping characteristics of cf RNA in serum from patients with liver cancer was described and a cf RNA signature with six cf RNAs was identified to diagnose liver cancer with high diagnostic efficiency(area under curve = 0.905 in the independent validation cohort) using this MOF method. Thus, this new MOF isolation technique will advance the field of liquid biopsy, with the potential to diagnose liver cancer. | Yuqing Sun Haixin Yu Shaoqing Han Ruoxi Ran Ying Yang Yongling Tang Yuhao Wang Wenhao Zhang Heng Tang Boqiao Fu Boshi Fu Xiaocheng Weng Song-Mei Liu Hexiang Deng Shuang Peng Xiang Zhou | 2024 | National Science Review2024，11，1： | 0 |
| 3 | 5-Formylcytosine and 5-Carboxylcytosine Significantly Reduce the Catalytic Activity of Hhal DNA Methyltransferase显示文摘 | Tingting Hong Fan Wu Boshi Fu Yushu Yuan Jinglei Xu Tianlu Wang Xiang Zhou | 2017 | Chinese Journal of Chemistry2017，35，6： | 0 |
| 4 | A pH-Activatable Fluorescent Aptamer Probe for Imaging of Target Cancer Cells显示文摘Early cancer diagnosis in molecular level shows great promise in relevant prevention and clinical treatment. Herein, we developed a novel method to detect and evaluate biomarkers on cancer cell surface. Based on the excellent selectivity of AS1411 aptamer to targeted nucleolin-overexpressed cells, we used a fluorescent probe with a pH-sensitive function to label the AS1411 aptamer modified with azide by click-reaction. The spectral characteristics of fluorophore naphthalimide has a good pH dependence. By this way, nucleolin-overexpressed cell could be discriminated from normal cell. Further, this strategy could also discriminate the breast cancer tissue from the adjacent benign tissue in formalin-fixed and parrffin-embedded(FFPE) tissue specimens. It is considered that our method has the potential to be applied in medical detection. | ZHOU Yan FU Boshi XIAO Heng | 2017 | Wuhan University Journal of Natural Sciences2017，22，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员