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| 1 | Inhibiting effect of antisense oligonucleotides phosphorthioate on gene expression of TIMP-1 in rat liver fibrosis显示文摘AIM To observe the inhibition of antisenseoligonucleotides (asON) phosphorthioate to thetissue inhibitors metalloproteinase-1 (TIMP-1)gene and protein expression in the liver tissue ofimmunologically induced hepatic fibrosis rats.The possibility of reversing hepatic fibrosisthrough gene therapy was observed.METHODS Human serum albumin (HSA) wasused to attack rats, as hepatic fibrosis model, inwhich asONs were used to block the gene andprotein expressing TIMP-1. According to theanalysis of modulator, structure protein, codingseries of TIMP-1 genome, we designed fourdifferent asONs. These asONs were injected intothe hepatic fibrosis models through coccygealvein. The results was observed by RT-PCR formeasuring TIMP-1 mRNA expression,immunohistochemistry and in situ hybridizationfor collagen Ⅰ, Ⅲ, special staining of collagenfiber, and electron microscopic examination.RESULTS Hepatic fibrosis could last within 363days in our modified model. The expressinglevel of TIMP-1 was high during hepatic fibrosisprocess. It has been proved by theimmunohistochemical and the electronmicroscopic examination that the asONphosphorthioate of TIMP-1 could exactly expressin vivo. The effect of colchicine wasdemonstrated to inhibit the expressing level ofmRNA and the content of collagen Ⅰ, Ⅲ in theliver of experimental hepatic fibrosis rats.However, the electron microscopy research andthe pathologic grading of hepatic fibrosisshowed that there was no significant differencebetween the treatment group and the modelgroup (P>0.05).CONCLUSION The experimental rat model ofhepatic fibrosis is one of the preferable modelsto estimate the curative effect of anti-hepaticfibrosis drugs. The asON phosphorthioate ofTIMP-1 could block the gene and proteinexpression of TIMP-1 in the liver of experimentalhepatic fibrosis rats at the mRNA level. It ispossible to reverse hepatic fibrosis, and it isexpected to study a new drug of anti-hepaticfibrosis on the genetic level. Colchicine has verylimited therapeutic effect on hepatic fibrosis,furthermore, its toxicity and side effects areobvious. | Qing He Nie Yong Qian Cheng Yu Mei Xie Yong Xing Zhou Yi Zhan Cao The Center of Infectious Disease Diagnosis and Treatment of PLA,Tangdu Hospital,Forth Military Medical University,Xi’an 710038,Shaanxi Province,ChinaDr,Qing He Nie graduated from Qinghai Medical College as a doctor in 1983,got master degree at Beijing 302 Army Hospital in 1993,got doctor degree at the Third Military Medical University in 1998,engaged in postdoctoral research at the Fourth Military Medical University from 1998 to 2000,now an associate professor,specialized in clinical and experimental research of infectious diseases,had more than 90 papers published,coauthor of ten books,first author of one book. | 2001 | World Journal of Gastroenterology2001，7，3： | 73 |
| 2 | Inflammatory pseudotumor of the liver: 13 cases of MRI findings显示文摘INTRODUCTIONInflammatory pseudotumor of the liver ,a rare benign lesion ,is often confused with the malignant tumors .Until 1998,less than 80 cases had been reported in the world [1,2].The accuracy of the pre-surgical diagnosis was low p[3,4] ,and very few materials on the diagnosis using dynamic MRI were reported [5-7] .We collected thirteen cases of inflammatory pseudotumor of the liver were collected and roved by pathology with MRI studies .Our purpose is to describe and analyze the MRI findings of this lesion and find out the valuable signs suggesting the diagnosis so as to avoid unnecessary operations. | Fu Hua Yan Kang Rong Zhou Ya Ping Jiang Wei Bin Shi Department of Radiology,Zhongshan Hospital,Medical Center of Fudan University (Former Shanghai Medical University),180 Fenglin Road,Shanghai 200032,ChinaDr.Fu Hua Yan graduated from Shanghai Medical University as a postgraduate in 1996,now an associated professor,a radiologist in chief specialized in the diagnosis of CT and MRI in hepatic diseases,having 22 papers published.She is the associated editor of the books Spiral CT and Body MRI. | 2001 | World Journal of Gastroenterology2001，7，3： | 8 |
| 3 | New Development of China-Russia-India Triangle显示文摘Since 2001, positive improvement has occurred in the trianglerelationship between China, Russia and India. The foreignministers of the three nations met in Russia recently, symbolizingthe launching of substantive triangular cooperation. | Ma Jiali is Research Professor at CICIR. He specializes in South Asian security, especially in India’s foreign policy. He is the author of three books, such as Pay Close Attention to India: A Rising Power which is very influential. He was one of the delegates for Track Two Dialogue between China and India for all of four rounds and a delegate for Track Two Dialogue between China, Russia and India. He is also a member of Eminent Persons Group Forum between China and India. | 2005 | Contemporary International Relations2005，15，7： | 4 |
| 4 | A proposed stan- dard system of nomenclature of human mitotic chro- mosomes (Denver, Colorado) 显示文摘 | Lejeune J Levan A Book JA | 1960 | The Lancet1960，275，7133： | 1 |
| 5 | Evaluation of posttransplant RhD sensitization in Rh D-negative renal recipients显示文摘 | Book K Agarwal A Filo S | | 0，，08： | 1 |
| 6 | The Acquisition and Use of an Internal Representation of the Spatial Layout of the Environment During Location显示文摘 | GARLING T BOOK A LINGDBERG E | 1979 | Man-Environment System1979，9，： | 1 |
| 7 | On the approximability of two capacitated vehicle routing problems 显示文摘 | Book A SanitL | 2013 | Electronic Notes in Discrete Mathematics2013，1，41： | 1 |
| 8 | Comparison and correlation of surface and sphenoidal electrodes with simultaneous intracranial recording: an interictal study 显示文摘 | Katz A Booke J | 1992 | EEG Clin Neurophysiol1992，82，： | 1 |
| 9 | Excessive insulin receptor serine phosphorylation in cultured fibroblasts and in skeletal muscle显示文摘 | Dunaif A Xia J Book CB | 1995 | J Clin Invest1995，96，2： | 1 |
| 10 | An epi-detected coherent anti-Stokes Raman scattering ( E-CARS) microscope with high spectral resolution andhigh sensitivity 显示文摘 | Cheng J X Volkmer A Book L D | 2001 | The Journal of Physical ChemistryB2001，105，7： | 1 |
| 11 | Outcome in emotionally related living kidney donor transplantation 显示文摘 | BINET I BOOK A H VOGELHACH P | 1997 | Nephrol Transplant1997，12，： | 1 |
| 12 | Multiplexcoherent anti-Stokes Raman scattering microspectroscopyand study of lipid vesicles 显示文摘 | Cheng J X Volkmer A Book L D | 2002 | The Journal of PhysicalChemistry B2002，106，34： | 1 |
| 13 | Time-resolved coherentanti-Stokes Raman scattering microscopy : Imaging basedon Raman free induction decay 显示文摘 | Volkmer A Book L D Xie X S | 2002 | Applied PhysicsLetters2002，80，9： | 1 |
| 14 | Modulation of the cost of pH regulation during metabolic depression: a ^31p NMR study in invertebrate (Sipunculus nudus) isolated muscle显示文摘 | Portner H O Book C Reipschlager A | 2000 | Journal of Experimental Biology2000，203，16： | 1 |
| 15 | Characterisation of porous hydrogen storage materials:carbons,zeolites,MOFs and PIMs显示文摘 | Tedds S Walton A Broomb D P Book D | | 0，，： | 1 |
| 16 | In vitro measurement accuracy of three - dimensional ultrasound 显示文摘 | Jotz ILl Tmbold T Book A | 2006 | Echocardiography2006，18，2： | 1 |
| 17 | Anaesthesia and the Elderly 显示文摘 | Sielenkanrnmper A booke Michael | 2001 | Current Opinion in Ansesthesiology2001，14，： | 1 |
| 18 | Anaesthesia and the elderly 显示文摘 | Sielenkamper A W Booke M | 2001 | Curr Opin Anaesthesiol2001，14，6： | 1 |
| 19 | Spatial orientation and wayfinding in the designed environment, a conceptual analysis and some suggestions for post occupancy evaluation显示文摘 | GarlingT Book A Lindberg E | 1986 | Journal of Architectural and Planning Research1986，3，1： | 1 |
| 20 | Excessive insulin receptor serinephosphorylation in cultured fibroblasts and in skeletal muscle apotential mechanism for insulin resistance in the polycystic ovarysyndrome显示文摘 | Dunaif A Xia J Book CB | 1995 | J Clin Invest1995，96，2： | 1 |
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