|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Identification of colorectal cancer metastasis markers by an angiogenesis-related cytokine-antibody array显示文摘AIM:To investigate the angiogenesis-related protein expression profile characterizing metastatic colorectal cancer (mCRC) with the aim of identifying prognostic markers.METHODS:The expression of 44 angiogenesissecreted factors was measured by a novel cytokine antibody array methodology.The study evaluated vascular endothelial growth factor (VEGF) and its soluble vascular endothelial growth factor receptor (sVEGFR)-1 protein levels by enzyme immunoassay (EIA) in a panel of 16 CRC cell lines.mRNA VEGF and VEGF-A isoforms were quantified by quantitative reverse-transcription polymerase chain reaction (Q-RT-PCR) and vascular endothelial growth factor receptor (VEGFR)-2 expression was analyzed by flow cytometry.RESULTS:Metastasis-derived CRC cell lines expressed a distinctive molecular profile as compared with those isolated from a primary tumor site.Metastatic CRC cell lines were characterized by higher expression of angiogenin-2 (Ang-2),macrophage chemoattractant proteins-3/4 (MCP-3/4),matrix metalloproteinase-1 (MMP-1),and the chemokines interferon γ inducible T cell α chemoattractant protein (I-TAC),monocyte chemoattractant protein I-309,and interleukins interleukin (IL)-2 and IL-1α,as compared to primary tumor cell lines.In contrast,primary CRC cell lines expressed higher levels of interferon γ (IFN-γ),insulin-like growth factor-1 (IGF-1),IL-6,leptin,epidermal growth factor (EGF),placental growth factor (PlGF),thrombopoietin,transforming growth factor β1 (TGF-β1) and VEGF-D,as compared with the metastatic cell lines.VEGF expression does not significantly differ according to the CRC cellular origin in normoxia.Severe hypoxia induced VEGF expression up-regulation but contrary to expectations,metastatic CRC cell lines did not respond as much as primary cell lines to the hypoxic stimulus.In CRC primary-derived cell lines,we observed a twofold increase in VEGF expression between normoxia and hypoxia as compared to metastatic cell lines.CRC cell lines express a similar pattern of VEGF isoforms (VEGF 121,VEGF 165 and VEGF 189) despite variability in VEGF expression,where the major transcript was VEGF 121.No relevant expression of VEGFR-2 was found in CRC cell lines,as compared to that of human umbilical vein endothelial cells and sVEGFR-1 expression did not depend on the CRC cellular origin.CONCLUSION:A distinct angiogenesis-related expression pattern characterizes metastatic CRC cell lines.Factors other than VEGF appear as prognostic markers and intervention targets in the metastatic CRC setting. | Ana Abajo Nerea Bitarte Ruth Zarate Valentina Boni Ines Lopez Marisol Gonzalez-Huarriz Javier Rodriguez Eva Bandres Jesus Garcia-Foncillas | 2012 | World Journal of Gastroenterology2012，18，7： | 8 |
| 2 | Pharmacogenomics in colorectal cancer: The first step for individualized-therapy显示文摘Interindividual differences in the toxicity and response to anticancer therapies are currently observed in practically all available treatment regimens. A goal of cancer therapy is to predict patient response and toxicity to drugs in order to facilitate the individualization of patient treatment. Identification of subgroups of patients that differ in their prognosis and response to treatment could help to identify the best available drug therapy according the genetic profile. Several mechanisms have been suggested to contribute to chemo-therapeutic drug resistance: amplification or overexpression of membrane transporters, changes in cellular proteins involved in detoxification or in DNA repair, apoptosis and activation of oncogenes or tumor suppressor genes. Colorectal cancer (CRC) is regarded as intrinsically resistant to chemotherapy. Several molecular markers predictive of CRC therapy have been included during the last decade but their results in different studies complicate their application in practical clinical. The simultaneous testing of multiple markers predictive of response could help to identify more accurately the true role of these polymorphisms in CRC therapy. This review analyzes the role of genetic variants in genes involved in the action mechanisms of the drugs used at present in colorectal cancer. | Eva Bandrés Ruth Zárate Natalia Ramirez Ana Abajo Nerea Bitarte Jesus García-Foncillas | 2007 | World Journal of Gastroenterology2007，13，44： | 4 |
| 3 | Moving forward in colorectal cancer research, what proteomics has to tell显示文摘Colorectal cancer is the third most common cancer and is highly fatal. During the last several years, research has been primarily based on the study of expression profiles using microarray technology. But now, investigators are putting into practice proteomic analyses of cancer tissues and cells to identify new diagnostic or therapeutic biomarkers for this cancer. Because the proteome reflects the state of a cell, tissue or organism more accurately, much is expected from proteomics to yield better tumor markers for disease diagnosis and therapy monitoring. This review summarizes the most relevant applications of proteomics the biomarker discovery for colorectal cancer. | Nerea Bitarte Eva Bandrés Ruth Zárate Natalia Ramirez Jesus Garcia-Foncillas | 2007 | World Journal of Gastroenterology2007，13，44： | 3 |
| 4 | microRNA-451 Regulates Macrophage Migration Inhibitory Factor Production and Proliferation of Gastrointestinal显示文摘 | Bandres E Bitarte N Arias F | 2009 | Cancer Cells2009，15，7： | 1 |
| 5 | microRNA - 451 reg- ulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells 显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，7： | 1 |
| 6 | Epigenetic regulation of microRNA expression in colorectal cancer显示文摘 | BANDRES E AGIRRE X BITARTE N | 2009 | Int J Cancer2009，125，11： | 1 |
| 7 | MicroRNA - 45! is involved in the self - renewal, tumorigenicity, and chemoresis- tance of colorectal cancer stem cells显示文摘 | BITARTE N BANDRES E BONI V | 2011 | Stem Cells2011，29，11： | 1 |
| 8 | MicroRNA-451 is involved in the self-renewal, lumorigenieity, and chemoresislanee of coloreetal cancer stem cells 显示文摘 | Bitarte N Bandres E Boni V | 2011 | Stem Cells2011，29，11： | 1 |
| 9 | Epigenetic regulation of microRNA expression in colorectal cancer显示文摘 | BANDRES E AGIRRE X BITARTE N | 2009 | International Journal of Cancer2009，125，11： | 1 |
| 10 | Epigenetic regulation of microRNA expression in colorectal cancer显示文摘 | Bandres E Agirre X Bitarte N | 2009 | Int J Cancer2009，125，11： | 1 |
| 11 | MicroRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，： | 1 |
| 12 | microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells 显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，7： | 1 |
| 13 | Epigenetic events in normal colonic mucosa surrounding colorectal cancer lesions显示文摘 | N. Ramírez E. Bandrés A. Navarro A. Pons S. Jansa I. Moreno F. Martínez-Rodenas R. Zárate N. Bitarte M. Monzó J. García-Foncillas | 2008 | European Journal of Cancer2008，，17： | 1 |
| 14 | micmRNA-d51 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells 显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，7： | 1 |
| 15 | microRNA2451 regulatesmacrophage migration inhibitory factor production and prolifera-tion of gast rointestinal cancer cells显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，： | 1 |
| 16 | gpigenetic regulation of mieroRNA expression in colorectal caneer显示文摘 | Bandres E Agirre X Bitarte N | 2009 | Int J Cancer2009，125，11： | 1 |
| 17 | miR-192/miR-215 influence 5-fluorouracil resistance through cell cycle-mediated mechanisms complementary to its post-transcriptional thymidilate synthase regulation显示文摘 | Boni V Bitarte N Cristobal I | 2010 | Mol Cancer Ther2010，9，： | 1 |
| 18 | microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells 显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，7： | 1 |
| 19 | microRNA-451 regulates macrophage migration inhibitory factor produc- tion and proliferation of gastrointestinal cancer cells 显示文摘 | Bandres E Bitarte N Arias F | 2009 | Clin Cancer Res2009，15，7： | 1 |
| 20 | Epigenetic regulation of microRNA expression in colorectal cancer显示文摘 | Bandres E Agirre X Bitarte N | | 0，，11： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员