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您的检索式：作者名="Benjamin EL.Li"
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| 1 | Differentiation potential of human adipose tissue derived stem cells into photoreceptors through explants culture and enzyme methods显示文摘● AIM: To investigate the retinal photoreceptor differentiation potential of human orbital adipose tissue-derived stem cells(ADSCs) generated by enzyme(EN) and explant(EX) culture methods.● METHODS: We investigated potentials of human orbital ADSCs to differentiate into photoreceptors through EN and EX culture methods. EN and EX orbital ADSCs were obtained from the same donor during rehabilitative orbital decompression, and then were subject to a 3-step induction using Noggin, DKK-1, IGF-1 and b-FGF at different time points for 38 d. Stem cell, eye-field and photoreceptor-related gene and protein markers were measured by reverse transcription-polymerase chain reaction(RT-PCR) and immunofluorescent(IMF) staining. ● RESULTS: Both EX and EN orbital ADSCs expressed CD133, a marker of cell differentiation. Moreover, PAX6 and rhodopsin, markers of the retinal progenitor cells, were detected from EX and EN orbital ADSCs. In EX orbital ADSCs, PAX6 m RNA was detected on the 17^(th) day and then the rhodopsin m RNA was detected on the 24^(th) day. In contrast, the EN orbital ADSCs expressed PAX6 and rhodopsin m RNA on the 31^(st) day. EX orbital ADSCs expressed rhodopsin protein on the 24^(th) day, while EN orbital ADSCs expressed rhodopsin protein on the 31^(st) day.● CONCLUSION: Orbital ADSCs isolated by direct explants culture show earlier and stronger expressions of markers towards eye field and retinal photoreceptor differentiation than those generated by conventional EN method. | Wei-Wei Xu Li Huang Kelvin K.L.Chong Doreen S.Y.Leung Benjamin EL.Li Zheng-Qin Yin Yi-Fei Huang Chi Pui Pang | 2017 | International Journal of Ophthalmology(English edition)2017，10，1： | 3 |
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