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您的检索式：作者名="Barry J.Shelp"
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| 1 | Targeted quantitative profiling of metabolites and gene transcripts associated with 4-aminobutyrate (GABA) in apple fruit stored under multiple abiotic stresses显示文摘4-Aminobutyrate accumulates in plants under abiotic stress.Here,targeted quantitative profiling of metabolites and transcripts was conducted to monitor glutamate-and polyamine-derived 4-aminobutyrate production and its subsequent catabolism to succinate or 4-hydroxybutyrate in apple(Malus x domestica Borkh.)fruit stored at 0°C with 2.5 kPa O2 and 0.03 or 5 kPa CO2 for 16 weeks.Low-temperature-induced protein hydrolysis appeared to be responsible for the enhanced availability of amino acids during early storage,and the resulting higher glutamate level stimulated 4-aminobutyrate levels more than polyamines.Elevated CO2 increased the levels of polyamines,as well as succinate and 4-hydroxybutyrate,during early storage,and 4-aminobutyrate and 4-hydroxybutyrate over the longer term.Expression of all of the genes likely involved in 4-aminobutyrate metabolism from glutamate/polyamines to succinate/4-hydroxybutyrate was induced in a co-ordinated manner.CO2-regulated expression of apple GLUTAMATE DECARBOXYLASE 2,AMINE OXIDASE 1,ALDEHYDE DEHYDROGENASE 10A8 and POLYAMINE OXIDASE 2 was evident with longer term storage.Evidence suggested that respiratory activities were restricted by the elevated CO2/O2 environment,and that decreasing NAD+availability and increasing NADPH and NADPH/NADP+,respectively,played key roles in the regulation of succinate and 4-hydroxybutyate accumulation.Together,these findings suggest that both transcriptional and biochemical mechanisms are associated with 4-aminobutyrate and 4-hydroxybutyrate metabolism in apple fruit stored under multiple abiotic stresses. | Carolyne J.Brikis Adel Zarei Greta Z.Chiu Kristen L.Deyman Jingyun Liu Christopher P.Trobacher Gordon J.Hoover Sanjeena Subedi Jennifer R.DeEll Gale G.Bozzo Barry J.Shelp | 2018 | Horticulture Research2018，5，1： | 1 |
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