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6篇 您的检索式:作者名="Barbara VALENT"
    题名 作者 年代 出处 被引量
1水稻抗稻瘟病菌防卫反应的细胞学分析与防卫基因表达显示文摘对水稻类病变坏死突变体lmm1(LmmKaty)与原亲本及双单倍体YT14和YT16接种稻瘟病菌24h内的抗病反应、细胞学和分子作用机理进行了研究。人工接种实验表明,与原亲本相比,该突变体对稻瘟病菌具有更强的抗性。突变体接种20~24h后,自动荧光检测到细胞死亡,而对照则未检测到。表明该突变体通过细胞程序性死亡阻止稻瘟病菌的蔓延,从而提高了抗病性。Northernblotting结果发现,抗病品系YT14中的防卫相关基因、苯丙氨酸解氨酶基因和β-葡聚糖酶基因在人工接种稻瘟病菌6h后开始表达,16~24h后上述基因表达量快速增加;而病程相关蛋白基因PR-1和几丁质酶基因在人工接种稻瘟病菌24h后才开始表达。与YT14相比,感病品系中上述基因的表达明显延迟。表明在人工接种稻瘟病菌24h内水稻可启动抗病防卫反应,从而对稻瘟病菌产生抗性。王忠华 林卉 Barbara VALENT J.Neil RUTGER 贾育林 2007中国水稻科学2007,21,4:5
2Host Active Defense Responses Occur within 24 Hours after Pathogen Inoculation in the Rice Blast System显示文摘Phenotypical, cytological and molecular responses of rice to the fungus Magnaporthe grisea were studied using rice cultivars and lesion mimic plants. The cultivar Katy was susceptible to several virulent M. grisea isolates, and a Sekiguchi like-lesion mimic mutant of Katy (LmmKaty) showed enhanced resistance to these isolates. Lesion mimic phenotype of LmmKaty was rapidly induced by virulent M. grisea isolates or by avirulent ones only at high levels of inoculum. Autofluorescence (a sign of an active defense response) was visible under ultraviolet light 24 h after localized inoculation in the incompatible interaction, whereas, not evident in the compatible interaction. Autofluorescence was also observed in LmmKaty 20 h after pathogen inoculation, indicating that rapid cell death is a mechanism of LmmKaty to restrict pathogen invasion. Rapid accumulations of defense related (DR) gene transcripts, phenylalanine ammonia lyase and R-glucanase, were observed beginning at 6 h and were obvious at 16 h and 24 h after inoculation in an incompatible interaction. Rapid transcript accumulations of PR-1 and chitinase had occurred by 24 h after inoculation in an incompatible interaction. Accumulations of these transcripts were delayed in the compatible interaction. These results indicate that host active defense responses occur 24 h after pathogen inoculation and that LmmKaty exhibits enhanced resistance to M. grisea. It is suggested that the autofluorescence and expression of the DR genes after heavy inoculation are important cytological and molecular markers respectively for early determination of the host response to M. grisea in the rice blast system.WANG Zhong-hua JIA Yu-lin LIN Hui Adair INTERN Barbara VALENT J. Neil RUTGER 2007Rice science2007,14,4:3
3Magnaporthe grisea Pthllp is a novel plasma membrane protein that mediates appressorium differentiation in response to inductive substrate cues显示文摘Todd M DeZwaan Anne M Carroll Barbara Valent 1999Plant Cell1999,11,10:1
4Mutations in Desmoglein-2 Gene Are Associated With Arrhythmogenic Right Ventricular Cardiomyopathy显示文摘Kalliopi Pilichou Andrea Nava Cristina Basso Giorgia Beffagna Barbara Bauce Alessandra Lorenzon Gianfranco Frigo Andrea Vettori Marialuisa Valente Jeffrey Towbin Gaetano Thiene Gian Antonio Danieli Alessandra Rampazzo 2006Circulation2006,,9:1
5Host-pathogen interactions in plants:plants,when exposed to oligosaccharides of fungal origin, defend themselves by accumulating antibiotics 显示文摘Albersheim P Barbara S Valent B S 1978The Journal of Cell Biology1978,78,3:1
6Mutations in Desmoglein-2 Gene Are Associated With Arrhythmogenic Right Ventricular Cardiomyopathy显示文摘Kalliopi Pilichou Andrea Nava Cristina Basso Giorgia Beffagna Barbara Bauce Alessandra Lorenzon Gianfranco Frigo Andrea Vettori Marialuisa Valente Jeffrey Towbin Gaetano Thiene Gian Antonio Danieli Alessandra Rampazzo 2006Circulation2006,,9:1
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