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| 1 | Plant species with extremely small populations(PSESP) in China:A seed and spore biology perspective显示文摘Approximately one fifth of the world's plants are at risk of extinction.Of these,a significant number exist as populations of few individuals,with limited distribution ranges and under enormous pressure due to habitat destruction.In China,these most-at-risk species are described as 'plant species with extremely small populations'(PSESP).Implementing conservation action for such listed species is urgent.Storing seeds is one of the main means of ex situ conservation for flowering plants.Spore storage could provide a simple and economical method for fern ex situ conservation.Seed and spore germination in nature is a critical step in species regeneration and thus in situ conservation.But what is known about the seed and spore biology(storage and germination) of at-risk species? We have used China's PSESP(the first group listing) as a case study to understand the gaps in knowledge on propagule biology of threatened plant species.We found that whilst germination information is available for 28 species(23%of PSESP),storage characteristics are only known for 8%of PSESP(10 species).Moreover,we estimate that 60%of the listed species may require cryopreservation for long-term storage.We conclude that comparative biology studies are urgently needed on the world's most threatened taxa so that conservation action can progress beyond species listing. | Ellie Merrett Wade Jayanthi Nadarajan Xiangyun Yang Daniel Ballesteros Weibang Sun Hugh W.Pritchard | 2016 | Plant Diversity2016，38，5： | 6 |
| 2 | Effect of low level laser therapy on dental pulp during orthodontic movement显示文摘AIM: To validate the protocol described here to be used in future clinical trials related to the effect of laser therapy on dental pulp. METHODS: Histologically treated samples from eight human healthy premolar teeth obtained from the middle root level were distributed in four groups: group 1(G1) absolute control; group 2(G2) only laser irradiation; group 3(G3) exposed only to orthodontics; and group 4(G4) treated with orthodontics and laser. Laser treatment was performed at 830 nm wavelength, 100 mW(energy 80 J/cm2, 2.2 J), for 22 s in the vestibular surface and 22 s in the palatal surface, 1 mm away from the dental root mucosa. Three staining methods were performed: hematoxylin-eosin(HE), Masson's Trichrome method and Gomori's method.RESULTS: The pulp histology parameters were evaluated and the results classified in to 3 parts: an inflammatory response, soft tissue response(dental pulp) and hard tissue response(dentin and predentin). There was no inflammation(chronic or acute) in any of the evaluated groups. The zones of pulp necrosis were found in one premolar of G3 and in one of G4; in groups G2 and G4 there was higher angiogenesis than in the other two groups. G4 group presented the highest level of vascularization. A reduced nerve density was observed in G3. A G2 specimen showed increased nerve density. A higher rate of calcification was observed in G1 compared to G2. Denticles, either real or false, were observed in G1, G2 and G3. Sclerosis of dentin and focal dentin loss was observed among all the groups. Secondary dentin was present in one sample in G1 and G2. A necrosis zone was found in one sample of G3 and G4. No differences between groups were observed in the odontoblast irregularity layer but the layer was wider in the group treated with laser only. A notable difference was detected in reduction of the cell-free layer between the groups G1 and G4. The findings in pulp tissue favor its adaptative response against dental movement induced by orthodontics. No definitive conclusions may be derived as this is a pilot study. CONCLUSION: The protocol described here was shown to be an effective method to evaluate changes in dental pulp submitted to low level laser in teeth under orthodontic movement. | ngela Domínguez Rosa Emilia Ballesteros Jairo Hernán Viáfara Oscar Mario Tamayo | 2013 | World Journal of Methodology2013，3，2： | 2 |
| 3 | Comparison of SHF and SSF processes from steam-exploded wheat straw for ethanol production by xylose-fermenting and robust glucose-fermenting Saccharomyces cerevisiae strains显示文摘 | TomasPejo E Oliva J M Ballesteros M | 2008 | Biotechnology and Bioengineering2008，100，6： | 1 |
| 4 | Phrasal translation and query expansion techniques for cross-language information retrieval 显示文摘 | BALLESTEROS L CROFT W B | 1997 | ACM SIGIR Forum1997，31，1： | 1 |
| 5 | Enhanced enzymatic hydrolysis of olive tree wood by steam explosion and alkaline peroxide delignification显示文摘 | Cristobal Cara Encamacion Ruiz Ignacio Ballesteros | 2006 | Process Biochemistry2006，41，2： | 1 |
| 6 | Lipase catalysed regioselective aeylation of sucrose in two- solvent mixtures 显示文摘 | M Ferrer M A Cruces M Bernabe A Ballesteros F J Plou | 1999 | Biotechnol Bioeng1999，65，： | 1 |
| 7 | Hemimicelles of alkyl carboxylates chemisorbed onto magnetic nanoparticles: study and application to the extraction of carcinogenic polycyclic aromatic hydrocarbons in environmental water samples 显示文摘 | Ballesteros - Gomez A Rubio S | 2009 | Anal Chem2009，81，： | 1 |
| 8 | Sensitive Gas Chromatographic-mass Spectrome-tric Method for the Determination of Phthalate Es-ters, Alkylphenols, Bisphenol A and Their Chlorina-ted Derivatives in Wastewater Samples显示文摘 | BALLESTEROS O ZAFRA A NACALON A etal | 2006 | J Chroma-togra A2006，1121，2： | 1 |
| 9 | Effects of salt stress on H + -ATPase activity of plasma membrane-enriched vesicles isolated from sunflower roots 显示文摘 | BALLESTEROS E KERKEB B PEDRO J | 1998 | Plant Science1998，134，： | 1 |
| 10 | Pretreat- ment technologies for an efficient hioethanol production process based on enzymatic hydrolysis: a review显示文摘 | Alvira P Tomds-Peje E Ballesteros M | 2010 | Bioresource Technology2010，101，13： | 1 |
| 11 | HE4,Ca125 and ROMA algorithm for differential diagnosis between benign gynaecological diseases and ovarian cancer显示文摘 | Ortiz-Muoz B Aznar-Oroval E García García A Covisa Peris A Perez Ballestero P Sanchez Yepes M | 2014 | Tumour Biol2014，35，7： | 1 |
| 12 | Field evaluation and agronomic performance of transgenic wheat显示文摘 | F. Barro P. Barceló P. Lazzeri P. Shewry A. Martín J. Ballesteros | 2002 | Theoretical and Applied Genetics (-)2002，，6： | 1 |
| 13 | Dilute sulfuric acid pretreatment of cardoon for ethanol produc- tion 显示文摘 | Ballesteros I Ballesteros M Manzanares P | 2008 | Biochemical Engineering Journal2008，42，1： | 1 |
| 14 | Combination of Phthalocyanine and Fullerene Moieties for Optical Limiting显示文摘 | Doyle J J Ballesteros B de la Torre G | 2006 | Chem Phys Lett2006，428，4： | 1 |
| 15 | Degradation of organophosphorous nerve agents by enzyme - polymer nanocomposites : efficient biocatalytic material for personal protection and large - scale detoxification显示文摘 | Gill L Ballesteros A | 2000 | Biotechnol Bioeng2000，70，4： | 1 |
| 16 | Structures of T cell immunoglobulin mucin receptors 1 and 2 reveal mechanisms for regulation of immune responses by the TIM receptor family显示文摘 | Ballesteros A Tami C | 2007 | Immunity2007，26，： | 1 |
| 17 | Reliability of the methods applied to assess age minority in living subjects around 18 years old A survey on a Moroccan origin population显示文摘 | Garamendi PM Landa MI Ballesteros J | 2005 | Forensic Science International2005，154，： | 1 |
| 18 | Effects of salt stress on H+ -ATPase and H+ -PPase activities of tonoplast-enriched vesicles isolated from sunflower roots 显示文摘 | BALLESTEROS E DONAIRE J P BELVER A | 1996 | Physiologia Plantarum1996，97，2： | 1 |
| 19 | Laccases and their app lications : A patent review 显示文摘 | Kunamneni A Plou F J Ballesteros A | 2008 | Recent Pat Biotechnol2008，2，1： | 1 |
| 20 | Effect of chip size on steam explosion pretreatment of softwood显示文摘 | I. Ballesteros J. M. Oliva A. A. Navarro A. González J. Carrasco M. Ballesteros | 2000 | Applied Biochemistry and Biotechnology (-)2000，，1： | 1 |
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