|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Bone-conditioned medium contributes to initiation and progression of osteogenesis by exhibiting synergistic TGF-β1/BMP-2 activity显示文摘Guided bone regeneration (GBR) often utilizes a combination of autologous bone grafts, deproteinized bovine bone mineral(DBBM), and collagen membranes. DBBM and collagen membranes pre-coated with bone-conditioned medium (BCM) extracted from locally harvested autologous bone chips have shown great regenerative potential in GBR. However, the underlying molecular mechanism remains largely unknown. Here, we investigated the composition of BCM and its activity on the osteogenic potential of mesenchymal stromal cells. We detected a fast and significant (P <0.001) release of transforming growth factor-β1 (TGF-β1) from autologous bone within 10 min versus a delayed bone morphogenetic protein-2 (BMP-2) release from 40 min onwards. BCMs harvested within short time periods (10, 20, or 40 min), corresponding to the time of a typical surgical procedure, significantly increased the proliferative activity and collagen matrix production of BCM-treated cells. Long-term (1, 3, or 6 days)-extracted BCMs promoted the later stages of osteoblast differentiation and maturation. Short-term-extracted BCMs, in which TGF-β1 but no BMP-2was detected, reduced the expression of the late differentiation marker osteocalcin. However, when both growth factors were present simultaneously in the BCM, no inhibitory effects on osteoblast differentiation were observed, suggesting a synergistic TGF-β1/BMP-2 activity. Consequently, in cells that were co-stimulated with recombinant TGF-β1 and BMP-2, we showed a significant stimulatory and dose-dependent effect of TGF-β1 on BMP-2-induced osteoblast differentiation due to prolonged BMP signaling and reduced expression of the BMP-2 antagonist noggin. Altogether, our data provide new insights into the molecular mechanisms underlying the favorable outcome from GBR procedures using BCM, derived from autologous bone grafts. | Maria B.Asparuhova Jordi Caballé-Serrano Daniel Buser Vivianne Chappuis | 2018 | International Journal of Oral Science2018，10，4： | 17 |
| 2 | Myocardial function and energy metabolism in carnitine-deficient rats显示文摘 | C. E. Zaugg M. Spaniol P. Kaufmann M. Bellahcene V. Barbosa M. Tolnay P. T. Buser S. Kr?henbühl | 2003 | Cellular and Molecular Life Sciences2003，，4： | 2 |
| 3 | Late clinical events after elopi- dogrel discontinuation may limit the benefit of drugeluting stents: an observational study of drug-eluting versus bare-metal stents 显示文摘 | PFISTERER M BRUNNER-LA ROCCA H P BUSER P T | 2006 | J Am Coll Cardiol2006，48，： | 1 |
| 4 | Pricing life of load rate caps on default-free adjustable rate mortgages显示文摘 | Buser A S Hendershotter P H | 1985 | AREUEA1985，13，3： | 1 |
| 5 | Soft tissue reactions to nonsubmerged unloaded titanium implants in beagle dogs显示文摘 | Buser D Weber HP Donath K | 1997 | J Periodontal1997，68，： | 1 |
| 6 | A gain-of-function mutation of JAK2 in myeloproliferative disorders显示文摘 | Kalovics R Passamonti F Buser AS | 2005 | N Engl J Med2005，352，17： | 1 |
| 7 | Optimizing esthetics for implant restorations in the anteriormaxilla:Anatomic and surgical consider- ations 显示文摘 | Buser D Martin W Belser UC | 2004 | Int J Oral Maxillofac Implants2004，19，： | 1 |
| 8 | Radiographic evaluation of crestal bone levels adjacent to non-submerged titanium implants显示文摘 | Weber HP Buser D Fiorellini JP | 1992 | Clin Oral Implants Res1992，3，4： | 1 |
| 9 | Formation of polychlofinated dibenzofurans (PCDFs) and polychlorinated dibenzo-pdioxins (PCDDs) from the pyrolysis of polychlorinated diphenyl ethers 显示文摘 | LINDAHL R RAPPE C BUSER H R | 1980 | Chemosphere1980，9，56： | 1 |
| 10 | Human cytomegalovirustegument protein ppUL35 is important for viral replication andparticle formation 显示文摘 | Schierling K Buser C Mertens T | 2005 | J Virol2005，79，5： | 1 |
| 11 | Surgical procedures in partially edentulous patients with ITI implants显示文摘 | Buser D Vonarx T | 2000 | Clin Oral Implants Res2000，11，11： | 1 |
| 12 | Horizontal ridge augmentation using autogenous block grafts and the guided bone regeneration technique with collagen membranes: a clinical study with 42 patients显示文摘 | Von Arx T Buser D | 2006 | Clin Oral Implants Res2006，17，4： | 1 |
| 13 | Federal Deposit Insurance, Regulatory Policy, and Optimal Bank Capital显示文摘 | Buser S A Chen A H Kane E J | 1981 | The Journal of Finance1981，，1： | 1 |
| 14 | The use of reduced healing times on ITI implants with a sandblasted and acid--etehed(SLA) surface: Early results from clinieal trials on ITISLA implants 显示文摘 | Coehran DL Buser D Bruggenkate C | 2002 | Clin Oral impl Res2002，13，： | 1 |
| 15 | Lightand transmission electron microscopy of the intact inter-faces between non-submerged titanium-coated epoxyresin implants in bone or gingiva 显示文摘 | Listgarten MA Buser D Steinemann SG | 1992 | Dent Rse1992，71，2： | 1 |
| 16 | Late clini-cal events after clopidogrel discontinuation may limit the benefitof drug-eluting stents:an observational study of drug-elutingversus bare-metal stents 显示文摘 | Pfisterer M Brunner-La Rocca HP Buser PT | 2006 | J Am Coll Cardiol2006，48，12： | 1 |
| 17 | Dose dependency of canthaxanthin crystals in monkey retina and spatial distribution of its metabolites显示文摘 | Goralczyk R Barker M Buser S | 2000 | Investigative ophthalmology and science2000，41，6： | 1 |
| 18 | Identification of polychlorinated dibenz o-p-dioxin isomers found in fly ash显示文摘 | BUSER H R BOSSHARDT H-P RAPPE C | 1978 | Chemosphere1978，7，2： | 1 |
| 19 | A Gain of Function Mutation of JAK2 in Myeloproliferative Disorders显示文摘 | Kralovics R Passamonti F Buser A S | 2005 | N Engl J Med2005，352，17： | 1 |
| 20 | Effect of low alcohol concentrations on visual attention in street traffic显示文摘 | Buser A Lachenmayr B Priemer F | 1996 | Ophthalmologe1996，93，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员