|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Mass transfer limitation of biotransformation:quantifying bioavailability显示文摘 | Bosma T N Middledorp P J M Schraa G | 1997 | Environmental Science and Technology1997，31，： | 1 |
| 2 | Steady - state and pre - steady - state kinetie analysis of halopropane conversion by a Rhodoeoceus haloalkane dehalogenase显示文摘 | Bosma T Pikkemaat M G Kingma J | 2003 | Biochemistry2003，42，26： | 1 |
| 3 | Ephrin A2 receptor targeting does not increase adenoviral pancreatic cancer transduction in vivo显示文摘AIM:To generate an adenoviral vector specifically targeting the EphA2 receptor(EphA2R) highly expressed on pancreatic cancer cells in vivo.METHODS:YSA,a small peptide ligand that binds the EphA2R with high affinity,was inserted into the HI loop of the adenovirus serotype 5 fiber knob.To further increase the specificity of this vector,binding sites for native adenoviral receptors,the coxsackie and adenovirus receptor(CAR) and integrin,were ablated from the viral capsid.The ablated retargeted adenoviral vector was produced on 293T cells.Specifi c targeting of this novel adenoviral vector to pancreatic cancer was investigated on established human pancreatic cancer cell lines.Upon demonstrating specifi c in vitro targeting,in vivo targeting to subcutaneous growing human pancreatic cancer was tested by intravenous and intraperitoneal administration of the ablated adenoviral vector.RESULTS:Ablation of native cellular binding sites reduced adenoviral transduction at least 100-fold.Insertion of the YSA peptide in the HI loop restored adenoviral transduction of EphA2R-expressing cells but not of cells lacking this receptor.YSA-mediated transduction was inhibited by addition of synthetic YSA peptide.The transduction specificity of the ablated retargeted vector towards human pancreatic cancer cells was enhanced almost 10-fold in vitro.In a subsequent in vivo study in a nude(nu/nu) mouse model however,no increased adenoviral targeting to subcutaneously growing human pancreas cancer nodules was seen upon injection into the tail vein,nor upon injection into the peritoneum.CONCLUSION:Targeting the EphA2 receptor increases specificity of adenoviral transduction of human pancreatic cancer cells in vitro but fails to enhance pancreatic cancer transduction in vivo. | Michael A van Geer Conny T Bakker Naoya Koizumi Hiroyuki Mizuguchi John G Wesseling Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，22： | 1 |
| 4 | Steady-stateand pre-steady- state kineticanalys is of halopropaneconversion by a Rhodococcus haloalkane dehalogenase 显示文摘 | Bosma T Pikkem aat M G Kingma J | 2003 | Biochemistry2003，42，26： | 1 |
| 5 | Mass transfer limitations of biotransforma-tion : quantifying bioavailability显示文摘 | Bosma T N P Middeldorp P J M Schraa G | 1997 | Environ Sci Technol1997，31，1： | 1 |
| 6 | En bloc removal of the lower lumbarvertebral body for chordoma: Report of two cases 显示文摘 | Bosma J J Pigott T J Pennie B H | 2001 | Neurosurg Spine2001，94，2： | 1 |
| 7 | Molecular analysis of rubella virus epidemiology across three continents, North America,Europe, and Asia 1961 - 1997 显示文摘 | Frey T K Abernathy E S Bosma T J | 1998 | J Infect Dis1998，178，3： | 1 |
| 8 | Novel Surface Display System for Proteins on Non-Genetically Modified Gram- Positive Bacteria显示文摘 | BOSMA T KANNINGA R NEEF J | 2006 | appl environ microbiol2006，72，1： | 1 |
| 9 | The use of mixing laws for modeling the climatic ageing of dielectric materials显示文摘 | BOSMA T J LENEY T CHENERIE I | 1995 | J Appl Phy D1995，28，6： | 1 |
| 10 | Mass transfer limitation of biotransformation:Quantifying bioavailability显示文摘 | Middeldorp P J Schraa G | 1997 | Environ Sci Technol1997，31，： | 1 |
| 11 | Mass transfer limitations of biotransformation:quantifying bioavailability显示文摘 | Bosma T N P P J M Middeldrop G Schraa | 1997 | Environmental Science & Technology1997，31，： | 1 |
| 12 | Dispersion of a continuously injected, nonlinearly adsorbing solute in chemically or physically heterogeneous porous formations显示文摘 | Bosma W J P Van der Zee S E A T M | 1995 | Journal of Contaminant Hydrology1995，18，： | 1 |
| 13 | Mophologic changes of exteahepatic bile dcct during obstruction andsubsequent decompressin byendoprosrhesis 显示文摘 | Karsten T M Bosma A Klopper P J | 1992 | Surgery1992，111，5： | 1 |
| 14 | Treponemal infections in hares in The Netherlands显示文摘 | LUMEIJ J T DE KONING J BOSMA R B | 1994 | J Clin Microbiol1994，32，2： | 1 |
| 15 | A tandem enzyme reaction to produce optical y active halohydrins,epoxides and diols显示文摘 | Spelberg J H L van Hylckama Vlieg J E T Bosma T | | 0，，： | 1 |
| 16 | Ex-vivo evaluation of gene therapy vectors in human pancreatic (cancer) tissue slices显示文摘AIM:To culture human pancreatic tissue obtained from small resection specimens as a pre-clinical model for examining virus-host interactions. METHODS:Human pancreatic tissue samples (malignant and normal)were obtained from surgical specimens and processed immediately to tissue slices. Tissue slices were cultured ex vivo for 1-6 d in an incubator using 95%O2.Slices were subsequently analyzed for viability and morphology.In addition the slices were incubated with different viral vectors expressing the reporter genes GFP or DsRed. Expression of these reporter genes was measured at 72 h after infection.RESULTS:With the Krumdieck tissue slicer,uniform slices could be generated from pancreatic tissue but only upon embedding the tissue in 3%low melting agarose.Immunohistological examination showed the presence of all pancreatic cell types.Pancreatic normal and cancer tissue slices could be cultured for up to 6 d,while retaining viability and a moderate to good morphology.Reporter gene expression indicated that the slices could be infected and transduced efficiently by adenoviral vectors and by adeno associated viral vectors,whereas transduction with lentiviral vectors was limited.For the adenoviral vector,the transduction seemed limited to the peripheral layers of the explants. CONCLUSION:The presented system allows reproducible processing of minimal amounts of pancreatic tissue into slices uniform in size,suitable for pre-clinical evaluation of gene therapy vectors. | Michael A van Geer Koert FD Kuhlmann Conny T Bakker Fibo JW ten Kate Ronald PJ Oude Elferink Piter J Bosma | 2009 | World Journal of Gastroenterology2009，15，11： | 1 |
| 17 | Mechanisms of inherited deficiencies of multiple UDPglucuronosyltransferase isoforms in tow patients with Crigler-Najjar syndrome, type I显示文摘 | BOSMA P J CHOWDHURY J R HUANG T J | 1992 | FASEB J1992，6，： | 1 |
| 18 | Mass transfer limitation of iotransformation:quantifying bioavailability显示文摘 | BOSMA T N P MIDDELDROP P J M SCHRAA G | 1997 | Environ Sci Technol1997，31，： | 1 |
| 19 | Molecular analysis of rubella virus epidemiology across three continents,North America,Europe and Asia,1961-1997显示文摘 | Frey T K Abemathy E S Bosma T J | 1998 | J Infect Dis1998，178，3： | 1 |
| 20 | Mass transfer limitation of biotransformation: quantifying bioavailability显示文摘 | Bosma T N Middledorp P J M Schraa G | 1997 | Environmental Science and Technology1997，31，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员