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| 1 | Colon cancer-associated B2 Escherichia coli colonize gut mucosa and promote cell proliferation显示文摘AIM:To provide further insight into the characterization of mucosa-associated Escherichia coli(E.coli)isolated from the colonic mucosa of cancer patients.METHODS:Phylogroups and the presence of cyclomodulin-encoding genes of mucosa-associated E.coli from colon cancer and diverticulosis specimens weredetermined by PCR.Adhesion and invasion experiments were performed with I-407 intestinal epithelial cells using gentamicin protection assay.Carcinoembryonic antigen-related cell adhesion molecule 6(CEACAM6)expression in T84 intestinal epithelial cells was measured by enzyme-linked immunosorbent assay and by Western Blot.Gut colonization,inflammation and procarcinogenic potential were assessed in a chronic infection model using CEABAC10 transgenic mice.Cell proliferation was analyzed by real-time mRNA quantification of PCNA and immunohistochemistry staining of Ki67.RESULTS:Analysis of mucosa-associated E.coli from colon cancer and diverticulosis specimens showed that whatever the origin of the E.coli strains,86%of cyclomodulin-positive E.coli belonged to B2 phylogroup and most harbored polyketide synthase(pks)island,which encodes colibactin,and/or cytotoxic necrotizing factor(cnf)genes.In vitro assays using I-407 intestinal epithelial cells revealed that mucosa-associated B2 E.coli strains were poorly adherent and invasive.However,mucosa-associated B2 E.coli similarly to Crohn’s disease-associated E.coli are able to induce CEACAM6expression in T84 intestinal epithelial cells.In addition,in vivo experiments using a chronic infection model of CEACAM6 expressing mice showed that B2 E.coli strain11G5 isolated from colon cancer is able to highly persist in the gut,and to induce colon inflammation,epithelial damages and cell proliferation.CONCLUSION:In conclusion,these data bring new insights into the ability of E.coli isolated from patients with colon cancer to establish persistent colonization,exacerbate inflammation and trigger carcinogenesis. | Jennifer Raisch Emmanuel Buc Mathilde Bonnet Pierre Sauvanet Emilie Vazeille Amélie de Vallée Pierre Déchelotte Claude Darcha Denis Pezet Richard Bonnet Marie-Agnès Bringer Arlette Darfeuille-Michaud | 2014 | World Journal of Gastroenterology2014，20，21： | 12 |
| 2 | Patients operated for tetralogy of fallot and with non-sustained ventricular tachycardia have reduced heart rate variability显示文摘 | Butera G Bonnet D Sidi D | 2004 | Herz2004，29，3： | 1 |
| 3 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietie cell显示文摘 | Bonnet D Dick I E | 1997 | N atureM ed1997，3，7： | 1 |
| 4 | Idebenone and reduced cardiac hypertrophy in Friedreieh's ataxia显示文摘 | Aggoun Y Bonnet D | 2002 | Heart2002，87，： | 1 |
| 5 | Effects of cadmium on the performance and microbiology of laboratory-scale lagoons treating domestic sewage显示文摘 | BONNET J L BOHATIER J PEPIN D | 1999 | Chemosphere1999，38，3： | 1 |
| 6 | Immune thrombocytopenic purpura after recombinant hepatitis B vaccine:retrospective study of seven cases显示文摘 | Neau D Bonnet F Michaud M | 1998 | Scand Infect Dis1998，30，2： | 1 |
| 7 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell显示文摘 | BONNET D DICK JE | 1997 | Nat Med1997，3，： | 1 |
| 8 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell 显示文摘 | Bonnet D DickJE | 1997 | Nat Med1997，3，7： | 1 |
| 9 | Distinctive NK-cell receptor repertoires sustain high-level constitutive NK-eell activation in HIV-exposed uninfected individuals 显示文摘 | Ravet S Scott-Atgara D Bonnet E | 2007 | Blood2007，109，10： | 1 |
| 10 | Biology of human bone marrow stem cells显示文摘 | BONNET D | 2003 | Clin Exp Med2003，3，3： | 1 |
| 11 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell显示文摘 | Bonnet D Dick JE | 1997 | Nature Med1997，3，7： | 1 |
| 12 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell显示文摘 | Bonnet D Dick JE | 1997 | Nat Med1997，3，7： | 1 |
| 13 | In vivo contribution of murine mesenchymal stem cells into multiple cell-types under minimal damage conditions显示文摘 | Anjos-Monso F Bonnet D | 2004 | J Cell Sci2004，117，23： | 1 |
| 14 | Risk of congenital heart defects associated with assisted reproductive technologies: a population-based evaluation 显示文摘 | Tararbit K Houyel L Bonnet D | 2011 | Eur Heart J2011，32，4： | 1 |
| 15 | Normal and leukaemic stem cells显示文摘 | Bonnet D | 2005 | Br J Haematol2005，130，4： | 1 |
| 16 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell 显示文摘 | Bonnet D Dick J E | 1997 | Nat Med1997，3，7： | 1 |
| 17 | Prophylactic lymph node dissection for papillary thyroid cancer less than 2 cm: im- plications for radioiodine treatment 显示文摘 | Bonnet S Hartl D Leboulleux S | 2009 | J Clin Endoerinol Metab2009，94，11： | 1 |
| 18 | Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell 显示文摘 | Bonnet D Dick JE | 1997 | Nat Med1997，3，： | 1 |
| 19 | 显示文摘 | Bonnet D Dick JE | 1997 | Nature Med1997，3，： | 1 |
| 20 | Stem cells of the alveolar epithelium显示文摘 | Griffiths MJ Bonnet D Janes SM | 2005 | Lancet2005，366，9481： | 1 |
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