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| 1 | Circulating tumor cells in pancreatic cancer patients:Enrichment and cultivation显示文摘AIM:To investigate the feasibility of separation and cultivation of circulating tumor cells(CTCs)in pancreatic cancer(Pa C)using a filtration device.METHODS:In total,24 Pa C patients who were candidates for surgical treatment were enrolled into the study.Peripheral blood samples were collected before an indicated surgery.For each patient,approximately8 m L of venous blood was drawn from the antecubitalveins.A new size-based separation Meta Cell?technology was used for enrichment and cultivation of CTCs in vitro.(Separated CTCs were cultured on a membrane in FBS enriched RPMI media and observed by inverted microscope.The cultured cells were analyzed by means of histochemistry and immunohistochemistry using the specific antibodies to identify the cell origin.RESULTS:CTCs were detected in 16 patients(66.7%)of the 24 evaluable patients.The CTC positivity did not reflect the disease stage,tumor size,or lymph node involvement.The same percentage of CTC positivity was observed in the metastatic and non-metastatic patients(66.7%vs 66.7%).We report a successful isolation of CTCs in Pa C patients capturing proliferating cells.The cells were captured by a capillary action driven size-based filtration approach that enabled cells cultures from the viable CTCs to be unaffected by any antibodies or lysing solutions.The captured cancer cells displayed plasticity which enabled some cells to invade the separating membrane.Further,the cancer cells in the'bottom fraction',may represent a more invasive CTC-fraction.The CTCs were cultured in vitro for further downstream applications.CONCLUSION:The presented size-based filtration method enables culture of CTCs in vitro for possible downstream applications. | Vladimir Bobek Robert Gurlich Petra Eliasova Katarina Kolostova | 2014 | World Journal of Gastroenterology2014，20，45： | 15 |
| 2 | Essentials of circulating tumor cells for clinical research and practice 显示文摘 | Liberko M Kolostova K Bobek V | 2013 | Crit Rev Oncol Hematol2013，88，： | 1 |
| 3 | Inhibitors of apoptosis confer resistance to tumour suppression by adoptively transplanted cytotoxic T-lymphocytes in vitro and in vivo 显示文摘 | Huber C Bobek N Kuball J | 2005 | Cell Death Differ2005，12，4： | 1 |
| 4 | The effect of dried tomato,grape and apple pomace on the cholesterolmetabolism and antioxidative enzymatic system in rats with hypercholesterolemia Nahrung显示文摘 | Bobek P Ozdin L Hromadova M | 1998 | Nahrung1998，42，5： | 1 |
| 5 | In vitro synergic antifungal effect of MUC7 12-met with histatin-5 12-met or miconazole 显示文摘 | Wei G X Bobek L A | 2004 | J Antimicrob Chemother2004，53，5： | 1 |
| 6 | Machines for continuous renal replacement therapy显示文摘 | Cruz D Bobek I Lentini P etl | 2009 | Semin Dial2009，22，2： | 1 |
| 7 | Antitumor and antimetastatic effect of warfarin and heparins显示文摘 | Bobek V Kovarik J | 2004 | Biomed Pharmacother2004，58，2： | 1 |
| 8 | Exogenous but not endogenous prostanoids regulate cytokine secretion from mu- rine bone marrow dendritic cells: EP2, DP, and IP but not EP1,EP3,and FP prostanoid receptors are involved显示文摘 | Jozefowski S Bobek M Marcinkiewicz J | 2003 | Int Immunopharmacol2003，3，6： | 1 |
| 9 | Service orientation in transitional markets:does it matter?显示文摘 | LYNN M L LYTLE R S BOBEK S | 2000 | European Journal of Marketing2000，34，3： | 1 |
| 10 | In vitro synergic antifungal effeet of MUC7 12 - met with histatin - 5 12 - met or miconazole显示文摘 | Wei GX Bobek LA | 2004 | J Antimicrob Chemother2004，53，5： | 1 |
| 11 | Intensive insulin therapy for critically ill patients 显示文摘 | Lewis KS Kane-Gill SL Bobek MB | 2004 | Ann Pharmacother2004，38，7： | 1 |
| 12 | Antitumor and antimetastatic effect of warfarin and heparins 显示文摘 | BOBEK V KOVA J | 2004 | Biomed Pharmacother2004，58，4： | 1 |
| 13 | Intensive insulin the-rapy for critically ill patients显示文摘 | LEWIS K S KANE-GILL S L BOBEK M B | 2004 | Ann Pharmacother2004，38，78： | 1 |
| 14 | Sur- gical treatment of insular tumours with tractography, func- tional magnetic resonance imaging, transcranial electrical stimulation and direct subcortical stimulation support 显示文摘 | Majchrzak K Bobek - Billewicz B Tymowski M | 2011 | Neurol Neurochir Pol2011，45，4： | 1 |
| 15 | MUC7 20 -Mer:investigation of antimicrobi- al activity, secondary structure, and possible mechanism of antifun- gal action显示文摘 | BOBEK L A SITU H | 2003 | Antimicrob Agents Chemother2003，47，2： | 1 |
| 16 | Leukotrienes modulate cytokine release from dendritic cells 显示文摘 | Jozefowski S Biodron R Bobek M | 2005 | Immunology2005，116，4： | 1 |
| 17 | MUC7 202Mer: investigation of antimicro-bial activity, secondary structure, and possible mechanism of antifungal ac-tion 显示文摘 | Bobek LA Situ H | 2003 | Antimicrobial Agentss Chemother2003，47，2： | 1 |
| 18 | Alterations in the expression of selected MHC antigens in premalignant lesions and squamous carcinomas of the uterine cervix显示文摘 | Chil A Sikorski M Bobek M | 2003 | Acta Obstet Gynecol Scand2003，82，12： | 1 |
| 19 | Integrating cancer genomics and proteomics in the post-genome era显示文摘 | Hanash SM Bobek MP Rickman DS | 2002 | Proteomics2002，2，1： | 1 |
| 20 | Ordered Quantum Dot Forma-tion on GaSb Surfaces during Ion Sputtering显示文摘 | Bobek T Facsko S Dekorsy T | 2001 | Nuclear In-struments and Methods in Physics Research2001，178，： | 1 |
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