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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Hepatitis C virus reinfection after liver transplantation: Is there a role for direct antiviral agents?显示文摘Recurrence of hepatitis C virus(HCV)infection following liver transplantation(LT)is almost universal and can accelerate graft cirrhosis in up to 30%of patients.The development of effective strategies to treat or prevent HCV recurrence after LT remains a major challenge,considering the shortage of donor organs and the accelerated progression of HCV in LT recipients.Standard antiviral therapy with pegylated-interferon plus ribavirin is the current treatment of choice for HCV LT recipients,even though the combination is not as effective as it is in immunocompetent patients.A sustained virological response in the setting of LT improves patient and graft survival,but this is only achieved in 30%-45%of patients and the treatment is poorly tolerated.To improve the efficacy of pre-and post-transplant antiviral therapy,a new class of potent direct-acting antiviral agents (DAAs)has been developed.The aim of this review is to summarize the use of DAAs in LT HCV patients.PubMed,Cochrane Library,MEDLINE,EMBASE,Web of Science and clinical trial databases were searched for this purpose.To date,only three clinical studies on the topic have been published and most of the available data are in abstract form.Although a moderately successful early virological response has been reported,DAA treatment regimens were associated with severe toxicity mitigating their potential usefulness.Moreover,the ongoing nature of data,the lack of randomized studies,the small number of enrolled patients and the heterogeneity of these studies make the results largely anecdotal and questionable.In conclusion,large welldesigned clinical studies on DAAs in HCV LT patients are required before these drugs can be recommended after transplantation. | Marco Dall’Agata Annagiulia Gramenzi Maurizio Biselli Mauro Bernardi | 2014 | World Journal of Gastroenterology2014，20，28： | 4 |
| 3 | Mierostructure and strength of Cu-Fe in situ composites after very high drawing strains 显示文摘 | Biselli C Morris D G | 1996 | Acta Mater1996，4，2： | 1 |
| 4 | 2007 Expression of heat shock protein 70 in a permanent cell line (EPC) exposed to sediment extracts from the North Sea and the Baltic Sea 显示文摘 | Kinder A Sierts-Herrmann A Biselli S | 2007 | Marine Environmental Reseach2007，63，： | 1 |
| 5 | Polymorphism C1420T of Sefine hydroxymethyltransferase gene on maternal risk for Down syndrome显示文摘 | Marucci GH Zampieri BL Biselli JM | 2012 | Mol Biol Rep2012，39，3： | 1 |
| 6 | 显示文摘 | Biselli C Morris D G Randdall N | 1994 | Seripta Metallurgieaet Materials1994，30，10： | 1 |
| 7 | The CGNDLRR type Rdg2a resistance gene confers immunity to the seed-borne barley leaf stripe pathogen in the absence of hypersensitive cell death 显示文摘 | Bulgarelli D Biselli C Collins N C | 2010 | PLoS ONE2010，5，12： | 1 |
| 8 | Microstrueture and strength of Cu-Fe in situ composites after very high drawing strains 显示文摘 | Biselli C Morris D G | 1996 | Acta Mater1996，4，2： | 1 |
| 9 | Mechanical alloying of high-strength copper alloys containing TiB2 and Al2O3 dispersoid particles显示文摘 | Biselli C Morris D G Randall N | 1994 | Scripta metallurgical material1994，30，10： | 1 |
| 10 | Genetic polymorphisms modulate the folate metabolism of Brazilian in- dividuals with Down syndrome 显示文摘 | Biselli JM Zampieri BL Goloni-Bertollo EM | 2012 | Molecular Biology Re- ports2012，39，10： | 1 |
| 11 | Inflammatory Myofibroblastic Tumor (Inflamina-tory Pseudotumor): DNA Flow Cyto metricanalysis of Nine Pediatric Cases显示文摘 | Biselli R Terlini C Fattorossi A | 1996 | Cancer1996，77，6： | 1 |
| 12 | Geneticpolymorphisms involved in folate metabolism and concen-trations of methylmalonic acid and folate on plasma homo-cysteine and risk of coronary artery disease 显示文摘 | Biselli PM Guerzoni AR de Godoy MF | 2010 | J ThrombThrombolysis2010，29，1： | 1 |
| 13 | Posttranscriptional changes of serum albumin: Clinical and prognostic significance in hospitalized patients with cirrhosis显示文摘 | Marco Domenicali Maurizio Baldassarre Ferdinando A Giannone Marina Naldi Marianna Mastroroberto Maurizio Biselli Maristella Laggetta Daniela Patrono Carlo Bertucci Mauro Bernardi Paolo Caraceni | 2014 | Hepatology2014，，6： | 1 |
| 14 | Microstructure and strength of Cu-Fe in situ composites after very high drawing strains显示文摘 | Morris D G | 1996 | Acta Mater1996，44，2： | 1 |
| 15 | Genetic Polymorp-hisms involved in folate metabolism and concentrations of methylmalonic acid and folate on plasma homocysteine and risk of coronary artery disease显示文摘 | Biselli PM Guerzoni AR de Godoy MF et a1 | 2010 | J Thromb Thrombolysis2010，29，1： | 1 |
| 16 | Regulation of normal human polyrnorphonuclear leucocytes by carnitine显示文摘 | FATTOROSSI A BISELLI R CASCIARO A | 1993 | Mediators Inflamm1993，2，： | 1 |
| 17 | Homo cysteineand MTHFR and VEGF gene polymorphisms, Impact on coronary ar- tery disease显示文摘 | Guerzoni AR Biselli PM Godoy MF | 2009 | Arq Bras Cardiol2009，92，4： | 1 |
| 18 | Mechanical alloying of highstrength copper alloys containing TiB2 and Al2O3 dispersoid particles 显示文摘 | Morris D G Randall N | 1994 | Scripta Metallurgica Material1994，30，10： | 1 |
| 19 | Genetic polymorphisms involved in folate metabolism and concen- trations of methylmalonic acid and folate on plasma homo- cysteine and risk of coronary artery disease 显示文摘 | Biselli PM Guerzoni AR de Godoy MF | 2010 | J Throm- bolysis2010，29，1： | 1 |
| 20 | Genetic polymor phisms involved in folate metabolism and concentrations of methylmalonic acid and folate plasma homocysteine and risk of coronary artery disease显示文摘 | Biselli PM Guerzoni AR Godoy MF | 2010 | Thromb Th rombolysis2010，29，： | 1 |
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