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| 1 | Simulation of shrinkage induced cracking in cement composite overlays 显示文摘 | JOHN E BOLANDER JR STEFANO BERTON | 2004 | Cement & Concrete Composites2004，26，： | 1 |
| 2 | A two-step EMG-and- optimization process to estimate muscle force during dynamic movement 显示文摘 | Amarantini D Rao G Berton E | 2010 | Journal of Biomechanics2010，43，： | 1 |
| 3 | Echocardiographic detection of early myocardial calcification in acute neonatal myocarditis due to Coxsackie virus type B显示文摘 | Benettoni A Berton E | 2011 | Pediatr Cardiol2011，32，7： | 1 |
| 4 | New approaches to antidepressant drug discovery:beyond monoamines显示文摘 | Berton O NestleR E J | 2006 | Nat Rev Neurosci2006，7，2： | 1 |
| 5 | Newapproaehes to antidepressant drug discovery: beyond monoamines 显示文摘 | BERTON O NESTLER E J | 2006 | Nat Rev Neurosci2006，7，2： | 1 |
| 6 | Determination of Clausius-Mossotti factors and surface capacitances for colloidal particles显示文摘 | HONEGGER T BERTON K PICARD E | 2011 | Applied Physics Letters2011，98，18： | 1 |
| 7 | Titanium anchors for the repair of distal Achilles tendon ruptures:preliminary report of a new surgical technique显示文摘 | Maniscalco P Bertone C Bonci E | 1998 | J Foot Ankle Surg1998，37，2： | 1 |
| 8 | Response of induced bone defects in horses to collagen matrix containing the human parathyroid hormone gene显示文摘 | Backstrom K C Bertone A L Wisner E R | 2004 | Am J Vet Res2004，65，9： | 1 |
| 9 | Titanium anchors for the re- pair of distal Achilles tendon ruptures: preliminary report of a new surgical technique显示文摘 | Maniscalco P Bertone C Bonci E | 1998 | The Journal of Foot and Ankle Surgery1998，37，2： | 1 |
| 10 | Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction显示文摘AIM: To investigate adenoviral transduction in mesenchymal stem cells(MSCs) and effects on stemness in vitro and function as a cell therapy in vivo.METHODS: Bone marrow-derived adult and fetal MSC were isolated from an equine source and expanded in monolayer tissue culture. Polyethylenimine(PEI)-mediated transfection of pc DNA3-e GFP or adenoviral transduction of green fluorescent protein(GFP) was evaluated in fetal MSCs. Adenoviral-mediated transduction was chosen for subsequent experiments. All experiments were carried out at least in triplicate unless otherwise noted. Outcome assessment was obtained by flow cytometry or immunohystochemistry and included transduction efficiency, cell viability, stemness(i.e., cell proliferation, osteogenic and chondrogenic cell differentiation), and quantification of GFP expression. Fetal and adult MSCs were then transduced with an adenoviral vector containing the gene for the bone morphogenic protein 2(BMP2). In vitro BMP2 expression was assessed by enzyme linked immunosorbent assay. In addition, MSC-mediated gene delivery of BMP2 was evaluated in vivo in an osteoinduction nude mouse quadriceps model. New bone formation was evaluated by microradiography and histology.RESULTS: PEI provided greater transfection and viability in fetal MSCs than other commercial chemical reagents. Adenoviral transduction efficiency was superior to PEI-mediated transfection of GFP in fetal MSCs(81.3% ± 1.3% vs 35.0% ± 1.6%, P < 0.05) and was similar in adult MSCs(78.1% ± 1.9%). Adenoviral transduction provided significantly greater expression of GFP in fetal than adult MSCs(7.4 ± 0.1 vs 4.4 ± 0.3 millions of mean fluorescence intensity units, P < 0.01) as well as significantly greater in vitro BMP2 expression(0.16 pg/cell-day vs 0.10 pg/cell-day, P < 0.01). Fraction of fetal MSC GFP positive cells decreased significantly faster than adult MSCs(1.15% ± 0.05% vs 11.4% ± 2.1% GFP positive at 2 wk post-transduction, P < 0.05). Cell proliferation and osteogenic differentiation in vitrowere not affected by Ad transduction in both fetal and adult MSCs, but fetal MSCs had reduced chondrogenic differentiation in vitro when compared to adult(P < 0.01). Chondrogenic differentiation was also significantly reduced in Ad-GFP transduced cells(P < 0.05). AdBMP2 transduced adult MSCs induced new bone formation in more thighs than Ad-BMP2 transduced fetal MSCs(83% vs 17% of the six treated thighs per group, P < 0.05) and resulted in increased femur midshaft diameter due to greater extent of periosteal new bone(1.57 ± 0.35 mm vs 1.27 ± 0.08 mm, P < 0.05).CONCLUSION: Fetal MSCs may be genetically manipulated ex vivo with adenoviral vectors. Nonetheless, the abbreviated expression of the exogenous gene may limit their applications in vivo. | Juan E Santiago-Torres Rebecca Lovasz Alicia L Bertone | 2015 | World Journal of Stem Cells2015，7，1： | 1 |
| 11 | Synthesis of TiO2 nanocrystals by nonhydrolytic solutionbased reactions显示文摘 | Trentler T J Denler T E Bertone J F | 1999 | J Am Chem Soc1999，121，7： | 1 |
| 12 | Issues in the analysis of oligonucleotide tiling microarrays for transcript mapping显示文摘 | Royce T E Rozowsky J S Bertone P | 2005 | Trends Genet2005，21，8： | 1 |
| 13 | Global identification of human transcribed sequences with genome Tiling arrays显示文摘 | Bertone P Stoic V Royce T E | 2004 | Science2004，306，24： | 1 |
| 14 | Prospective study of recreational physical activity and ovarian cancer显示文摘 | Bertone E R Willett W C Rosner V B A | 2001 | J Natl Cancer2001，93，： | 1 |
| 15 | Synthesis of TiO2 nano- crystals by nonhydrolytic solution-based reactions 显示文摘 | Trentler T J Denler T E Bertone J F | 1999 | Journal of the American Chemical Society1999，121，7： | 1 |
| 16 | Changes of ascor- bic acid and surface color of green peas sterilized in cans sub- jected to end-over-end agitation 显示文摘 | Garrote R L Silva E R Bertone R A | 2006 | Journal of Food Engi- neering2006，73，： | 1 |
| 17 | Successive use of amphiphilic blockcopolymers as nanoreactors and templates:preparation of porous silica with metal nanoparticles显示文摘 | BRONSTEIN L KRAMER E BERTON B | 1999 | Chem Mater1999，11，： | 1 |
| 18 | Prediction of the Optimum Harvest Time of 'Scarlet' Apples Using DR-UV-Vis and NIR Spectroscopy 显示文摘 | Bertone E Venturello A Leari R | 2012 | Postharvest Biology and Technology2012，69，： | 1 |
| 19 | Judging where a ball will go: The ease of curved free-kicks in football 显示文摘 | Craig C M Berton E Rao G | 2006 | Na- ture Wissenschaften2006，，2： | 1 |
| 20 | Optic expert varia- bles used to judge future ball arrival position in and novice soccer players 显示文摘 | Craig C M Goulon C Berton E | 2009 | Attention Perception and Psychophys- ics2009，71，： | 1 |
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