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| 1 | Visualizing the microtubule-associated protein tau in the nucleus显示文摘Although tau is mainly known as an axonal microtubule-associated protein,many studies indicate that it is not restricted to this subcellular compartment.Assessing tau’s subcellular distribution,however,is not trivial as is evident from transgenic mouse studies.When human tau is over-expressed,it can be immunohistochemically localized to axons and the somatodendritic domain,modeling what is found in neurodegenerative diseases such as Alzheimer’s disease.Yet,in wild-type mice,despite its abundance,tau is difficult to visualize even in the axon.It is even more challenging to detect this protein in the nucleus,where tau has been proposed to protect DNA from damage.To establish a framework for future studies into tau’s nuclear functions,we compared several methods to visualize endogenous nuclear tau in cell lines and mouse brain.While depending on the fixation and permeabilization protocol,we were able to detect nuclear tau in SH-SY5Y human neuroblastoma cells,we failed to do so in N2a murine neuroblastoma cells.As a second method we used subcellular fractionation of mouse tissue and found that in the nucleus tau is mainly present in a hypophosphorylated form.When either full-length or truncated human tau was expressed,both accumulated in the cytoplasm,but were also found in the nuclear fraction.Because subcellular fractionation methods have their limitations,we finally isolated nuclei to probe for nuclear tau and found that the nuclei were free of cytoplasmic contamination.Together our analysis identifies several protocols for detecting tau in the nucleus where it is found in a less phosphorylated form. | LU Jing LI Ting HE RongQiao BARTLETT Perry F GTZ Jürgen | 2014 | Science China(Life Sciences)2014，57，4： | 11 |
| 2 | Transcriptome profile of human neuroblastoma cells in the hypomagnetic field显示文摘Research has shown that the hypomagnetic field(HMF)can affect embryo development,cell proliferation,learning and memory,and in vitro tubulin assembly.In the present study,we aimed to elucidate the molecular mechanism by which the HMF exerts its effect,by comparing the transcriptome profiles of human neuroblastoma cells exposed to either the HMF or the geomagnetic field.A total of 2464 differentially expressed genes(DEGs)were identified,216 of which were up-regulated and2248 of which were down-regulated after exposure to the HMF.These DEGs were found to be significantly clustered into several key processes,namely macromolecule localization,protein transport,RNA processing,and brain function.Seventeen DEGs were verified by real-time quantitative PCR,and the expression levels of nine of these DEGs were measured every 6 h.Most notably,MAPK1 and CRY2,showed significant up-and down-regulation,respectively,during the first 6 h of HMF exposure,which suggests involvement of the MAPK pathway and cryptochrome in the early bio-HMF response.Our results provide insights into the molecular mechanisms underlying the observed biological effects of the HMF. | MO WeiChuan LIU Ying BARTLETT Perry F HE RongQiao | 2014 | Science China(Life Sciences)2014，57，4： | 8 |
| 3 | Introduction to the thematic issue “From brain function to therapy”显示文摘This thematic issue of Science China Life Sciences,'From brain function to therapy',highlights some of the exciting research being undertaken in the Joint Laboratory of Neuroscience and Cognition,an international scientific collaboration between the Queensland Brain Institute(QBI)at | BARTLETT Perry F HE RongQiao | 2014 | Science China(Life Sciences)2014，57，4： | 7 |
| 4 | The role of the N-methyl-D-aspartate receptor in the proliferation of adult hippocampal neural stem and precursor cells显示文摘New neurons are continuously generated from resident pools of neural stem and precursor cells(NSPCs)in the adult brain.There are multiple pathways through which adult neurogenesis is regulated,and here we review the role of the N-methyl-D-aspartate receptor(NMDAR)in regulating the proliferation of NSPCs in the adult hippocampus.Hippocampal-dependent learning tasks,enriched environments,running,and activity-dependent synaptic plasticity,all potently up-regulate hippocampal NSPC proliferation.We first consider the requirement of the NMDAR in activity-dependent synaptic plasticity,and the role the induction of synaptic plasticity has in regulating NSPCs and newborn neurons.We address how specific NMDAR agonists and antagonists modulate proliferation,both in vivo and in vitro,and then review the evidence supporting the hypothesis that NMDARs are present on NSPCs.We believe it is important to understand the mechanisms underlying the activation of adult neurogenesis,given the potential that endogenous stem cell populations have for repopulating the hippocampus with functional new neurons.In conditions such as age-related memory decline,neurodegeneration and psychiatric disease,mature neurons are lost or become defective;as such,stimulating adult neurogenesis may provide a therapeutic strategy to overcome these conditions. | TAYLOR Chanel J HE RongQiao BARTLETT Perry F | 2014 | Science China(Life Sciences)2014，57，4： | 6 |
| 5 | Load Factor Calibration for the Proposed 2005 Edition of the National Building Code for Canada: Companion-action Load Combinations 显示文摘 | Bartlett F M Hong H P Zhou W | 2003 | Canadian Journal of Civil Engineering2003，，30： | 1 |
| 6 | Spinning magnets and Jehle's model of the electron显示文摘 | Bartlett D F Monroy J Reeves J | 1977 | Phys Rev D1977，16，： | 1 |
| 7 | Kinetics of dolomitization显示文摘 | Sibley D F Dedoes R E Bartlett T R | 1987 | Geology1987，15，： | 1 |
| 8 | SWI/SNF chroma-tin -remodeling factors induce changes in DNA methylation topromote transcriptional activation 显示文摘 | Banine F Bartlett C Gunawardena R | 2005 | Cancer Res2005，65，9： | 1 |
| 9 | Medulloblastoma显示文摘 | Bartlett F Kortmann R Saran F | 2013 | Clin Oncol(R Coll Radiol)2013，25，1： | 1 |
| 10 | Cloning and Growth of Multioptential Neural Precursors-Requirements for Proliferation and Differentiation显示文摘 | Kilpatrick T J Bartlett P F | 1993 | Neuron1993，10，2： | 1 |
| 11 | A checklist for critical appraisal of studies of biological variation显示文摘 | BARTLETT WA BRAGA F CAROBENE A | 2015 | Clin Chem Lab Med2015，53，6： | 1 |
| 12 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates显示文摘 | Bartlett K S Mckeith F K Vandehaar M J | 2006 | J Anim Sci2006，84，： | 1 |
| 13 | Solute accumulation in the deep-sea bacterium Photobacterium profundum显示文摘 | Martin D D Bartlett D H Roberts M F | 2002 | Extremophiles2002，6，： | 1 |
| 14 | Growth and body composition of dairy calves fed milk replacers containing different amounts of protein at two feeding rates 显示文摘 | Bartlett K S McKeith F K VandeHaar M J | 2006 | Journal of Animal Science2006，，84： | 1 |
| 15 | Imported fire ant toxic bait studies:eyaluatiOil of toxicants显示文摘 | Stringer C E Lofgren C S Bartlett F J | 1964 | J Econ Entomo1964，57，： | 1 |
| 16 | Modal verification of Force determination for measuring vibration loads显示文摘 | Bartlett F D Flannelly W D | 1979 | Journal of the American Helicopter Society1979，，： | 1 |
| 17 | Intravenous iron alone for the treatment of anemia in patients with chronic heart failure显示文摘 | BOLGER A P BARTLETT F R PENSTON H S | 2006 | J Am Coll Cardiol2006，48，： | 1 |
| 18 | SWI/SNF chromatin-remodeling factors induce changes in DNA methylation to promote transcriptional activation 显示文摘 | Banine F Bartlett C Gunawardena R | 2005 | Cancer Res2005，65，9： | 1 |
| 19 | Communityacquired pneumonia in adults: guidelines for management,The infection Diseases society of America 显示文摘 | Bartlett J G Breiman R F Mandell L A | 1998 | Clin Infect Dis1998，26，4： | 1 |
| 20 | 显示文摘 | Bartlett R J Stanton J F | 1994 | Rev Comp Chem1994，，5： | 1 |
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