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| 1 | Helicobacter pylori in dental plaque and stomach of patients from Northern Brazil显示文摘AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA polymorphisms.RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical outcome.CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth. | Mnica Baraúna Assumpo Luisa Caricio Martins Hivana Patricia Melo Barbosa Katarine Antonia dos Santos Barile Sintia Silva de Almeida Paulo Pimentel Assumpo Tereza Cristina de Oliveira Corvelo | 2010 | World Journal of Gastroenterology2010，16，24： | 29 |
| 2 | Interleukin-1 and TNF-α polymorphisms and Helicobacter pylori in a Brazilian Amazon population显示文摘AIM:To study the association between Interleukin-1(IL-1)and tumor necrosis factor(TNF)-αpolymorphisms,infection by Helicobacter pylori(H pylori)and the development of gastrointestinal diseases.METHODS:Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers.The polymorphisms in IL-1βand TNF-αgenes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method(PCRRFLP)and those from IL-1RN with PCR.The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology.The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS:A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL1RN*2 was more frequent among patients with gastric ulcers and adenocarcinoma.Carriers of the allele ILRN*2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma,as well as a higher degree of inflammation and neutrophilic activity in the gastric mucosa.CONCLUSION:Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma. | Hivana Patricia Melo Barbosa Luisa Caricio Martins Sidney Emanuel Batista dos Santos Samia Demachki Mnica Baraúna Assumpo Charliana Damasceno Arago Tereza Cristina de Oliveira Corvelo | 2009 | World Journal of Gastroenterology2009，15，12： | 17 |
| 3 | Impact of laparoscopic surgery training laboratory on surgeon's performance显示文摘Minimally invasive surgery has been replacing the open standard technique in several procedures. Similar or even better postoperative outcomes have been described in laparoscopic or robot-assisted procedures when compared to open surgery. Moreover, minimally invasive surgery has been providing less postoperative pain, shorter hospitalization, and thus a faster return to daily activities. However, the learning curve required to obtain laparoscopic expertise has been a barrier in laparoscopic spreading. Laparoscopic surgery training laboratory has been developed to aid surgeons to overcome the challenging learning curve. It may include tutorials, inanimate model skills training(box models and virtual reality simulators), animal laboratory, and operating room observation. Several different laparoscopic courses are available with specific characteristics and goals. Herein, we aim to describe the activities performed in a dry and animal-model training laboratory and to evaluate the impact of different kinds of laparoscopic surgery training courses on surgeon's performance. Several tasks are performed in dry and animal laboratory to reproduce a real surgery. A short period of training can improve laparoscopic surgical skills, although most of times it is not enough to confer laparoscopic expertise for participants. Nevertheless, this short period of training is able to increase the laparoscopic practice of surgeons in their communities. Full laparoscopic training in medical residence or fellowship programs is the best way of stimulating laparoscopic dissemination. | Fabio C M Torricelli Joao Arthur B A Barbosa Giovanni S Marchini | 2016 | World Journal of Gastrointestinal Surgery2016，8，11： | 6 |
| 4 | Determination of the furaltadone metabolite 5-methylmorpholino-3-amino -9 oxazolidinone (AMOZ) using liquid chromatography coupled to electrospray tandem mass spectrometry during the nitrofuran crisis in Portugal显示文摘 | Barbosa J Ferreira M L Ramos F | 2007 | Accred Qual Assur2007，12，： | 1 |
| 5 | Improving hierarchical cluster analysis: a new method with outlier detection and automatic clustering显示文摘 | ALMEIDA J A S BARBOSA L M S PAIS A A C C | 2007 | Chemometrics and Intelligent Labora- tory Systems2007，87，2： | 1 |
| 6 | Leptin effect on RANKL and OPG expression in MC3T3-E1 osteoblasts 显示文摘 | Lamghari M Tavares L Camboa N Barbosa M A | 2006 | J Cell Biochem2006，98，5： | 1 |
| 7 | Aggregation and clay dispersion of an oxisol treated with swine and poultry manures 显示文摘 | BARBOSA GMC OLIVEIRA J F MIYAZAWA M | 2015 | Soil & Tillage Research2015，146，： | 1 |
| 8 | Hepatic photosensitization in buffaloes intoxicated by Bra- chiaria decumbens in Minas Gerais state, Brazil显示文摘 | De Oliveira C H S Barbosa J D Oiiveira C M C | 2013 | Toxi- con2013，73，： | 1 |
| 9 | Cellulose phosphates as biomaterials mineralization of chemically modi?ed regenerated cellulose hydrogels显示文摘 | Granja P L Barbosa M A | 2001 | Journal of Materials Science2001，36，1： | 1 |
| 10 | Characterization of Ba- cillus pmbiotics available for human use显示文摘 | Due le H Hong H A Barbosa T M | 2004 | Appl Environ Micro- biol2004，70，4： | 1 |
| 11 | Polypropylene/Polystyrene Blends: In Situ Compatibilization by Friedel-Crafts Alkylation Reaction 显示文摘 | Diaz M F Barbosa S E Capiati N | 2004 | Journal of Polymer Science: Part B: Polymer Physics2004，42，： | 1 |
| 12 | Pharmacological and phytochemical studies of callus culture extracts from Alternanthera brasiliana 显示文摘 | Macedo A F Barbosa N C Esquibel M A | 1999 | Pharmazie1999，54，10： | 1 |
| 13 | Computational anal- ysis of thoracic multideteetor row HRCT for segmentation and quantification of small airway air trapping and emphysema in ob- structive pulmonary disease 显示文摘 | Barbosa E M Jr Song G Tustison N | 2011 | Acad Radiol2011，18，10： | 1 |
| 14 | Access to safe abortion:building choices for women living with HIV and AIDS 显示文摘 | Orner PJ De Bruyn M Barbosa RM | 2011 | J Int AIDS Soc2011，14，： | 1 |
| 15 | First report of Tomato chlorosis virus infecting tomato crops in Brazil 显示文摘 | Barbosa J C Teixeira A P M Moreira A G el al | 2008 | Plant Disease2008，92，12： | 1 |
| 16 | Effect of carotenoid source and dietary lipid content on blood astaxanthin concentration in rainbow trout ( Oncorhynehus mykiss) 显示文摘 | BARBOSA M J MORAl S R CHOUBERT | 1999 | Aquaculture1999，176，34： | 1 |
| 17 | Potassium-use efficiency in common bean genotypes 显示文摘 | FAGERIA N K BARBOSA COSTA M P | 2001 | Journal of Plant Nutrition2001，24，12： | 1 |
| 18 | Screening of yeasts for production of xylitol from D-xylose and some factors which affect xylitol yield in Candida guilliermondii显示文摘 | BARBOSA M F S MEDEIROS M B MANCILHA I M | 1988 | Journal of Industrial Microbiology1988，3，： | 1 |
| 19 | Ethnicity affects the distribution of delta-aminolevulinic acid dehydratase (ALAD) genetic variants显示文摘 | MONTENEGRO M F BARBOSA F JR SANDRIM V C | 2006 | Clin Chim Acta2006，367，： | 1 |
| 20 | Differential expression of over 60 chromosomal genes in Escherichia coli by constitutive expres- sion of MarA显示文摘 | Barbosa T M Levy S B | 2000 | J Bacheriol2000，182，12： | 1 |
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