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| 1 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 2 | Symmetry of cardiac function assessment显示文摘正确、左的室从主要的心试管的二个邻近的片断被开发。尽管他们关于形状和力量是不同的,他们以行为反射对方。这是在心脏的功能评价的对称的第一水平。心肌收缩和松驰是活跃的。这在心脏的功能评价构造对称的第二水平。二个层次的联合将帮助发现一些隐藏的索引或途径评估心脏的功能。在这篇文章,从 echocardiography 的四个主要指数在这本金,十七个指数或测量途径下面被分析从阴影出来了,它在对心脏的功能的评价是很有用的,特别为恰好心脏的功能和心脏舒张的心脏的功能。 | Xu-Fang BAI Amy X MA | 2016 | Journal of Geriatric Cardiology2016，13，6： | 4 |
| 3 | Isolation and characterization of neural stem cells from human fetal striatum显示文摘 | Li X Xu J Bai Y Wang X Dai X Liu Y Zhang J Zou J Shen L Li L | 2006 | 中国生物学文摘2006，20，5： | 3 |
| 4 | Metabotropic glutamate receptor antagonists and agonists: potential neuroprotectors in diffuse brain injury 显示文摘 | Fei Z Zhang X Bai HM | 2006 | J Clin Neurosci2006，13，10： | 1 |
| 5 | Development of real-time PCRassays for the quantitative detection of Epmein-barr virus and Cytmegaloviru8, comparison of taqman pmpmbes, and molecular显示文摘 | Jebbink J Bai X Rogers BB | 2003 | Mo L Diagn2003，5，1： | 1 |
| 6 | Deflagration to detonation transition in aluminum dust-air mixture under weak ignition condition显示文摘 | LIU Q LI X BAI C | 2009 | Combustion and Flame2009，156，4： | 1 |
| 7 | Mutational analysis of thyroid transcription factor-1 gene (TTF-1) in lung carcino- mas显示文摘 | BAI X Y SHEN H | 2008 | In Vitro Cell Dev Biol-Animal2008，44，： | 1 |
| 8 | Detection of pandemic influenza A/ H1N1/2009 virus by real-time reverse transcription polymerase chain reaction显示文摘 | Wu W Kang X Bai Z | 2010 | J Virol Methods2010，165，2： | 1 |
| 9 | The Electro- chemical reaction mechanism of selenocystine on seleni-um-gold film modified glassy carbon electrode 显示文摘 | BAI Y YAN X L ZHENG W J | 2006 | Colloids and Surfaces B : Biointerfaces2006，49，： | 1 |
| 10 | Haploinsufficiency of p18INK4c sensitizes mice to carcinogen-induced tumorigenesis显示文摘 | BAI F PEI X H VIRGINIA L G | 2003 | Molecular and Cellular2003，23，4： | 1 |
| 11 | Electrochemical and XPS studies on the aqueous corrosion resistance of niobium-implanted zircaloy-4显示文摘 | Chen X W Bai X D Xu J | 2002 | Journal of Materials Science Letters2002，21，22： | 1 |
| 12 | The evidences of human orphan receptor COUP-TFII inhibiting telomerase activity through decreasing hTERT transcription 显示文摘 | Wang Q Bai Z Li X | 2004 | Cancer Lett2004，214，1： | 1 |
| 13 | Development of real-time PCR assays for the quantitative detection of Epstein-barr virus and Cytomegalovirus,comparison of taqman proprobes,and molecular beacons显示文摘 | Jebbink J Bai X Rogers BB | 2003 | J Mol Diagn2003，5，1： | 1 |
| 14 | Narrow-band interfe-rence suppression for SAR based on complex empirical mode decomposition显示文摘 | ZHOU F XING M BAI X | 2009 | IEEE Geoscience and Remote Sensing Letters2009，6，3： | 1 |
| 15 | Key factors in mTOR Regulation显示文摘 | Bai X Jiang Y | 2010 | Ceil Mol Life Sci2010，67，： | 1 |
| 16 | Induction of IL-8 by mycoplasmapneumoniae membrane in BEAS-2B cells显示文摘 | Chmura K Bai X Nakamura M | 2008 | Am J Physiol LungCell Mol Physiol2008，，： | 1 |
| 17 | Preparation and Properties of Cu-10Sn Alloy Infiltrated 316L Stainless Steel Composites 显示文摘 | Yang X Bai Y L Xu M | 2012 | Advanced Materials Research2012，503，504： | 1 |
| 18 | Application of independ- ent component analysis to AC dipole based optics meas- urement and correlation at the relativistic heavy ion colli- der 显示文摘 | Shen X Lee S Y Bai M | 2013 | Physical Review Special Topics : Accelerators and Beams2013，16，11： | 1 |
| 19 | Learning context-sensitive shape similarity by graph transduction 显示文摘 | BAI X YANG X LATECKI L J | 2010 | IEEE Transactions on Pattern Analysis and Machine Intelligence2010，32，5： | 1 |
| 20 | The prediction of well user's behavior based on well usage mining显示文摘 | BAI X L CHEN F | 2010 | ETP/IITA Confer- ence on Telecommunication and Information (TEIN 2010)2010，10，1： | 1 |
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