2篇
您的检索式：作者名="B.R.Challa"
|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Quantification of sibutramine and its two metabolites in human plasma by LC-ESI-MS/MS and its application in a bioequivalence study显示文摘Obesity can be considered as a chronic illness of epidemic proportion and its incidents have increased exponentially in recent years. The use of anti-obesity drugs such as sibutramine is somewhat helpful. There is a need to quantify such drugs in biological samples, which is generally quite difficult. In this report, we developed and validated a simple, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of sibutramine (SB) and its two metabolites N-des methyl sibutramine (DSB) and N-di desmethyl sibutramine (DDSB) in human plasma. Zorbax SBC18 (4.6 mm 75 mm, 3.5 mm, 80 A) analytical column and 5 mM ammonium formate:acetonitrile (10:90, v/v) mobile phase were used for chromatographic separation of SB, DSB and DDSB. Multiple reaction monitoring (MRM) in the positive mode was used to detect SB, DSB and DDSB at m/z 280.3/124.9, 266.3/ 125.3 and 252.2/124.9, respectively. Liquid-liquid extraction was used for the extraction of analytes and internal standard from human plasma. This method was validated over a linear concentration range of 10.0-10,000.0 pg/mL for SB, DSB and DDSB with correlation coefficients (r) of Z0.9997. The drug and the two metabolites were stable in plasma samples. The validated method was successfully applied in a bioequivalence and pharmacokinetic study with human volunteers under fasting condition. | Venkata Suresh Ponnuru B.R.Challa RamaRao Nadendla | 2012 | Journal of Pharmaceutical Analysis2012，2，4： | 6 |
| 2 | Bio-analytical method development and validation of Rasagiline by high performance liquid chromatography tandem mass spectrometry detection and its application to pharmacokinetic study显示文摘The most suitable bio-analytical method based on liquid-liquid extraction has been developed and validated for quantification of Rasagiline in human plasma.Rasagiline-13C3 mesylate was used as an internal standard for Rasagiline.Zorbax Eclipse Plus C18(2.1 mm×50 mm,3.5 μm) column provided chromatographic separation of analyte followed by detection with mass spectrometry.The method involved simple isocratic chromatographic condition and mass spectrometric detection in the positive ionization mode using an API-4000 system.The total run time was 3.0 min.The proposed method has been validated with the linear range of 5-12000 pg/mL for Rasagiline.The intra-run and inter-run precision values were within 1.3%-2.9% and 1.6%-2.2% respectively for Rasagiline.The overall recovery for Rasagiline and Rasagiline-13 C 3 mesylate analog was 96.9% and 96.7% respectively.This validated method was successfully applied to the bioequivalence and pharmacokinetic study of human volunteers under fasting condition. | Ravi Kumar Konda Babu Rao Chandu B.R.Challa Chandrasekhar B.Kothapalli | 2012 | Journal of Pharmaceutical Analysis2012，2，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员